LOCUS       sequence-367728-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human TTR in E coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Sun X, Ferguson JA, Dyson HJ, Wright PE
  TITLE     A transthyretin monomer intermediate undergoes local unfolding and 
            transient interaction with oligomers in a kinetically concerted 
            aggregation pathway.
  JOURNAL   J Biol Chem. 2022 Jun 18;298(8):102162. doi: 
            10.1016/j.jbc.2022.102162.
  PUBMED    35724960
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-06-18"; volume: "298(8):102162. doi"; pages: " 
            10.1016/j.jbc.2022.102162"
COMMENT     Sequence Label: TTR C10S E63C
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(470..488)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      484..531
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgggt cctacgggca ccggtgaatc
       61 caagagtcct ctgatggtca aagttctaga tgctgtccga ggcagtcctg ccatcaatgt
      121 ggccgtgcat gtgttcagaa aggctgctga tgacacctgg gagccatttg cctctgggaa
      181 aaccagtgag tctggagagc tgcatgggct cacaactgag gagtgctttg tagaagggat
      241 atacaaagtg gaaatagaca ccaaatctta ctggaaggca cttggcatct ccccattcca
      301 tgagcatgca gaggtggtat tcacagccaa cgactccggc ccccgccgct acaccattgc
      361 cgccctgctg agcccctact cctattccac cacggctgtc gtcaccaatc ccaaggaatg
      421 agatccggct gctacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      481 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      541 ggaactatat ccggattggc gaatgggacg
//
