LOCUS       sequence-36773-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: Erk1
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..109
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaat aattttgttt aactttaaga aggagatata catatgcacc atcatcatca
       61 tcattcttct ggtgtagatc tgggtaccga gaacctgtac ttccaatcca atgcggcggc
      121 ggcggctcag gggggcgggg gcggggagcc ccgtagaacc gagggggtcg gcccgggggt
      181 cccgggggag gtggagatgg tgaaggggca gccgttcgac gtgggcccgc gctacacgca
      241 gttgcagtac atcggcgagg gcgcgtacgg catggtcagc tcggcctatg accacgtgcg
      301 caagactcgc gtggccatca agaagatcag ccccttcgaa catcagacct actgccagcg
      361 cacgctccgg gagatccaga tcctgctgcg cttccgccat gagaatgtca tcggcatccg
      421 agacattctg cgggcgtcca ccctggaagc catgagagat gtctacattg tgcaggacct
      481 gatggagact gacctgtaca agttgctgaa aagccagcag ctgagcaatg accatatctg
      541 ctacttcctc taccagatcc tgcggggcct caagtacatc cactccgcca acgtgctcca
      601 ccgagatcta aagccctcca acctgctcat caacaccacc tgcgacctta agatttgtga
      661 tttcggcctg gcccggattg ccgatcctga gcatgaccac accggcttcc tgacggagta
      721 tgtggctacg cgctggtacc gggccccaga gatcatgctg aactccaagg gctataccaa
      781 gtccatcgac atctggtctg tggg
//
