LOCUS       sequence-367733-       11122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human BAFFR-CFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Sevdali E, Block V, Lataretu M, Li H, Smulski CR, Briem JS, Heitz Y,
            Fischer B, Ramirez NJ, Grimbacher B, Jack HM, Voll RE, Holzer M, 
            Schneider P, Eibel H
  TITLE     BAFFR activates PI3K/AKT signaling in human naive but not in 
            switched memory B cells through direct interactions with B cell 
            antigen receptors.
  JOURNAL   Cell Rep. 2022 Jun 28;39(13):111019. doi: 
            10.1016/j.celrep.2022.111019.
  PUBMED    35767961
REFERENCE   2  (bases 1 to 11122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-06-28"; volume: "39(13):111019. doi"; pages: " 
            10.1016/j.celrep.2022.111019"
COMMENT     Sequence Label: pNL-CEF-BAFFR-CFP
FEATURES             Location/Qualifiers
     source          1..11122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2719..3901
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2950..3892
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3849..3869
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(4867..4886)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     LTR             5373..6006
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7646..7663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7817..8405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7897..7916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8576..9367,9368..9436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9199..9218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9437..9541)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9609..9627
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9665..9687)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9787..9806
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagtta actagatcta
     2401 gagtccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attacccggc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2761 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2941 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3001 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
     3061 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3121 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3181 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3241 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3301 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3361 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3421 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3481 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3541 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3601 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     3661 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     3721 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3781 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3841 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     3901 aggaattgtt aattaaccgg ctagaattcc cgggatcgca gcttgtgcgg cggcgtcggc
     3961 accatgaggc gagggccccg gagcctgcgg ggcagggacg cgccagcccc cacgccctgc
     4021 gtcccggccg agtgcttcga cctgctggtc cgccactgcg tggcctgcgg gctcctgcgc
     4081 acgccgcggc cgaaaccggc cggggccagc agccctgcgc ccaggacggc gctgcagccg
     4141 caggagtcgg tgggcgcggg ggccggcgag gcggcgctgc ccctgcccgg gctgctcttt
     4201 ggcgcccccg cgctgctggg cctggcactg gtcctggcgc tggtcctggt gggtctggtg
     4261 agctggaggc ggcgacagcg gcggcttcgc ggcgcgtcct ccgcagaggc ccccgacgga
     4321 gacaaggacg ccccagagcc cctggacaag gtcatcattc tgtctccggg aatctctgat
     4381 gccacagctc ctgcctggcc tcctcctggg gaagacccag gaaccacccc acctggccac
     4441 agtgtccctg tgccagccac agagctgggc tccactgaac tggtgaccac caagaccgcc
     4501 ggccctgagc aacaaggctc cggatctggc agcggctctg gctccggcag cggaagcggc
     4561 agcgtgagca agggcgagga gctgttcacc ggcgtggtgc ccatcctggt ggagctggac
     4621 ggcgacgtga acggccacaa gttcagcgtg agcggcgagg gcgagggcga cgccacctac
     4681 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4741 ctggtgacta ccctgacctg gggcgtgcag tgcttcgcca ggtatcccga ccacatgaag
     4801 cagcacgact tcttcaagag cgccatgccc gagggctacg tgcaggagag gaccatcttc
     4861 ttcaaggacg acggcaacta caagaccagg gccgaggtga agttcgaggg cgacaccctg
     4921 gtgaacagga tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctgggccac
     4981 aagctggagt acaacgccat cagcgacaac gtgtacatca ccgccgacaa gcagaagaac
     5041 ggcatcaagg ccaacttcaa gatcaggcac aacatcgagg acggcagcgt gcagctggcc
     5101 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5161 tacctgagca cccagagcaa gctgagcaag gaccccaacg agaagaggga ccacatggtg
     5221 ctgctggagt tcgtgaccgc cgccggcatc accctgggca tggacgagct gtacaagtag
     5281 taaactagtg gatccatcga tggtaccttt aagaccaatg acttacaagg cagctgtaga
     5341 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag
     5401 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     5461 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     5521 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     5581 ccctgtgagc ctgcatggaa tggatgaccc tgagagagaa gtgttagagt ggaggtttga
     5641 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     5701 ctgacatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     5761 gggcgggact ggggagtggc gagccctcag atgctgcata taagcagctg ctttttgcct
     5821 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5881 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5941 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6001 ctagcaccca ggaggtagag gttgcagtga gccaagatcg cgccactgca ttccagcctg
     6061 ggcaagaaaa caagactgtc taaaataata ataataagtt aagggtatta aatatattta
     6121 tacatggagg tcataaaaat atatatattt gggctgggcg cagtggctca cacctgcgcc
     6181 cggccctttg ggaggccgag gcaggtggat cacctgagtt tgggagttcc agaccagcct
     6241 gaccaacatg gagaaacccc ttctctgtgt atttttagta gattttattt tatgtgtatt
     6301 ttattcacag gtatttctgg aaaactgaaa ctgtttttcc tctactctga taccacaaga
     6361 atcatcagca cagaggaaga cttctgtgat caaatgtggt gggagaggga ggttttcacc
     6421 agcacatgag cagtcagttc tgccgcagac tcggcgggtg tccttcggtt cagttccaac
     6481 accgcctgcc tggagagagg tcagaccaca gggtgagggc tcagtcccca agacataaac
     6541 acccaagaca taaacaccca acaggtccac cccgcctgct gcccaggcag agccgattca
     6601 ccaagacggg aattaggata gagaaagagt aagtcacaca gagccggctg tgcgggagaa
     6661 cggagttcta ttatgactca aatcagtctc cccaagcatt cggggatcag agtttttaag
     6721 gataacttag tgtgtagggg gccagtgagt tggagatgaa agcgtaggga gtcgaaggtg
     6781 tccttttgcg ccgagtcagt tcctgggtgg gggccacaag atcggatgag ccagtttatc
     6841 aatccggggg tgccagctga tccatggagt gcagggtctg caaaatatct caagcactga
     6901 ttgatcttag gttttacaat agtgatgtta ccccaggaac aatttgggga aggtcagaat
     6961 cttgtagcct gtagctgcat gactcctaaa ccataatttc ttttttgttt tttttttttt
     7021 atttttgaga cagggtctca ctctgtcacc taggctggag tgcagtggtg caatcacagc
     7081 tcactgcagc ctcaacgtcg taagctcaag cgatcctccc acctcagcct gcctggtagc
     7141 tgagactaca agcgacgccc cagttaattt ttgtattttt ggtagaggca gcgttttgcc
     7201 gtgtggccct ggctggtctc gaactcctgg gctcaagtga tccagcctca gcctcccaaa
     7261 gtgctgggac aaccggggcc agtcactgca cctggcccta aaccataatt tctaatcttt
     7321 tggctaattt gttagtccta caaaggcagt ctagtcccca ggcaaaaagg gggtttgttt
     7381 cgggaaaggg ctgttactgt ctttgtttca aactataaac taagttcctc ctaaacttag
     7441 ttcggcctac acccaggaat gaacaaggag agcttggagg ttagaagcac gatggaattg
     7501 gttaggtcag atctctttca ctgtctgagt tataattttg caatggtggt tcaaagactg
     7561 cccgcttctg acaccagtcg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9541 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9601 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     9661 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     9721 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     9781 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9841 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     9901 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccaggggagg cagagattgc
     9961 agtaagctga gatcgcagca ctgcactcca gcctgggcga cagagtaaga ctctgtctca
    10021 aaaataaaat aaataaatca atcagatatt ccaatctttt cctttattta tttatttatt
    10081 ttctattttg gaaacacagt ccttccttat tccagaatta cacatatatt ctatttttct
    10141 ttatatgctc cagttttttt tagaccttca cctgaaatgt gtgtatacaa aatctaggcc
    10201 agtccagcag agcctaaagg taaaaaataa aataataaaa aataaataaa atctagctca
    10261 ctccttcaca tcaaaatgga gatacagctg ttagcattaa ataccaaata acccatcttg
    10321 tcctcaataa ttttaagcgc ctctctccac cacatctaac tcctgtcaaa ggcatgtgcc
    10381 ccttccgggc gctctgctgt gctgccaacc aactggcatg tggactctgc agggtcccta
    10441 actgccaagc cccacagtgt gccctgaggc tgccccttcc ttctagcggc tgcccccact
    10501 cggctttgct ttccctagtt tcagttactt gcgttcagcc aaggtctgaa actaggtgcg
    10561 cacagagcgg taagactgcg agagaaagag accagcttta cagggggttt atcacagtgc
    10621 accctgacag tcgtcagcct cacagggggt ttatcacatt gcaccctgac agtcgtcagc
    10681 ctcacagggg gtttatcaca gtgcaccctt acaatcattc catttgattc acaatttttt
    10741 tagtctctac tgtgcctaac ttgtaagtta aatttgatca gaggtgtgtt cccagagggg
    10801 aaaacagtat atacagggtt cagtactatc gcatttcagg cctccacctg ggtcttggaa
    10861 tgtgtccccc gaggggtgat gactacctca gttggatctc cacaggtcac agtgacacaa
    10921 gataaccaag acacctccca aggctaccac aatgggccgc cctccacgtg cacatggccg
    10981 gaggaactgc catgtcggag gtgcaagcac acctgcgcat cagagtcctt ggtgtggagg
    11041 gagggaccag cgcagcttcc agccatccac ctgatgaaca gaacctaggg aaagccccag
    11101 ttctacttac accaggaaag gc
//
