LOCUS       sequence-367735-       11293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human CD79B as RFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Sevdali E, Block V, Lataretu M, Li H, Smulski CR, Briem JS, Heitz Y,
            Fischer B, Ramirez NJ, Grimbacher B, Jack HM, Voll RE, Holzer M, 
            Schneider P, Eibel H
  TITLE     BAFFR activates PI3K/AKT signaling in human naive but not in 
            switched memory B cells through direct interactions with B cell 
            antigen receptors.
  JOURNAL   Cell Rep. 2022 Jun 28;39(13):111019. doi: 
            10.1016/j.celrep.2022.111019.
  PUBMED    35767961
REFERENCE   2  (bases 1 to 11293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-06-28"; volume: "39(13):111019. doi"; pages: " 
            10.1016/j.celrep.2022.111019"
COMMENT     Sequence Label: pNL-CEF-CD79B-RFP
FEATURES             Location/Qualifiers
     source          1..11293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2719..3901
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2950..3892
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3849..3869
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4635..4644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4641..5354
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     LTR             5544..6177
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7817..7834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7988..8576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8068..8087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8747..9538,9539..9607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9370..9389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9608..9712)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9780..9798
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9836..9858)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9958..9977
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagtta actagatcta
     2401 gagtccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attacccggc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2761 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2941 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3001 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
     3061 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3121 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3181 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3241 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3301 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3361 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3421 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3481 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3541 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3601 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     3661 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     3721 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3781 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3841 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     3901 aggaattgtt aatcaccatg gccaggctgg cgttgtctcc tgtgcccagc cactggatgg
     3961 tggcgttgct gctgctgctc tcagcagctg agccagtacc agcagccaga tcggaggacc
     4021 ggtaccggaa tcccaaaggt agtgcttgtt cgcggatctg gcagagccca cgtttcatag
     4081 ccaggaaacg gggcttcacg gtgaaaatgc actgctacat gaacagcgcc tccggcaatg
     4141 tgagctggct ctggaagcag gagatggacg agaatcccca gcagctgaag ctggaaaagg
     4201 gccgcatgga agagtcccag aacgaatctc tcgccaccct caccatccaa ggcatccggt
     4261 ttgaggacaa tggcatctac ttctgtcagc agaagtgcaa caacacctcg gaggtctacc
     4321 agggctgcgg cacagagctg cgagtcatgg gattcagcac cttggcacag ctgaagcaga
     4381 ggaacacgct gaaggatggt atcatcatga tccagacgct gctgatcatc ctcttcatca
     4441 tcgtgcctat cttcctgctg ctggacaagg atgacagcaa ggctggcatg gaggaagatc
     4501 acacctacga gggcctggac attgaccaga cagccaccta tgaggacata gtgacgctgc
     4561 ggacagggga agtgaagtgg tctgtaggtg agcacccagg ccaggaggac gggagtggga
     4621 gcgatccacc ggtcgccacc atggtgtcta agggcgaaga gctgattaag gagaacatgc
     4681 acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg
     4741 gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc
     4801 ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc agaaccttca
     4861 tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag ggcttcacat
     4921 gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc
     4981 tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg
     5041 gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg ctgtaccccg
     5101 ctgacggcgg cctggaaggc agaagcgaca tggccctgaa gctcgtgggc gggggccacc
     5161 tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
     5221 ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac aaagagacct
     5281 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc
     5341 acaaacttaa ttgagcggcc gcgactctag atcataatca gccataccac atttgtagag
     5401 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     5461 gcaattgttg ttggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5521 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa gacaagatat
     5581 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     5641 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     5701 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     5761 cctgcatgga atggatgacc ctgagagaga agtgttagag tggaggtttg acagccgcct
     5821 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgacatcg
     5881 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     5941 tggggagtgg cgagccctca gatgctgcat ataagcagct gctttttgcc tgtactgggt
     6001 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6061 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6121 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaccc
     6181 aggaggtaga ggttgcagtg agccaagatc gcgccactgc attccagcct gggcaagaaa
     6241 acaagactgt ctaaaataat aataataagt taagggtatt aaatatattt atacatggag
     6301 gtcataaaaa tatatatatt tgggctgggc gcagtggctc acacctgcgc ccggcccttt
     6361 gggaggccga ggcaggtgga tcacctgagt ttgggagttc cagaccagcc tgaccaacat
     6421 ggagaaaccc cttctctgtg tatttttagt agattttatt ttatgtgtat tttattcaca
     6481 ggtatttctg gaaaactgaa actgtttttc ctctactctg ataccacaag aatcatcagc
     6541 acagaggaag acttctgtga tcaaatgtgg tgggagaggg aggttttcac cagcacatga
     6601 gcagtcagtt ctgccgcaga ctcggcgggt gtccttcggt tcagttccaa caccgcctgc
     6661 ctggagagag gtcagaccac agggtgaggg ctcagtcccc aagacataaa cacccaagac
     6721 ataaacaccc aacaggtcca ccccgcctgc tgcccaggca gagccgattc accaagacgg
     6781 gaattaggat agagaaagag taagtcacac agagccggct gtgcgggaga acggagttct
     6841 attatgactc aaatcagtct ccccaagcat tcggggatca gagtttttaa ggataactta
     6901 gtgtgtaggg ggccagtgag ttggagatga aagcgtaggg agtcgaaggt gtccttttgc
     6961 gccgagtcag ttcctgggtg ggggccacaa gatcggatga gccagtttat caatccgggg
     7021 gtgccagctg atccatggag tgcagggtct gcaaaatatc tcaagcactg attgatctta
     7081 ggttttacaa tagtgatgtt accccaggaa caatttgggg aaggtcagaa tcttgtagcc
     7141 tgtagctgca tgactcctaa accataattt cttttttgtt tttttttttt tatttttgag
     7201 acagggtctc actctgtcac ctaggctgga gtgcagtggt gcaatcacag ctcactgcag
     7261 cctcaacgtc gtaagctcaa gcgatcctcc cacctcagcc tgcctggtag ctgagactac
     7321 aagcgacgcc ccagttaatt tttgtatttt tggtagaggc agcgttttgc cgtgtggccc
     7381 tggctggtct cgaactcctg ggctcaagtg atccagcctc agcctcccaa agtgctggga
     7441 caaccggggc cagtcactgc acctggccct aaaccataat ttctaatctt ttggctaatt
     7501 tgttagtcct acaaaggcag tctagtcccc aggcaaaaag ggggtttgtt tcgggaaagg
     7561 gctgttactg tctttgtttc aaactataaa ctaagttcct cctaaactta gttcggccta
     7621 cacccaggaa tgaacaagga gagcttggag gttagaagca cgatggaatt ggttaggtca
     7681 gatctctttc actgtctgag ttataatttt gcaatggtgg ttcaaagact gcccgcttct
     7741 gacaccagtc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7921 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7981 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8041 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8101 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8161 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8221 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8281 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8341 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8401 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8461 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8521 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8581 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8641 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8701 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8761 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8821 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8881 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8941 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9001 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9061 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9121 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9181 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9241 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9301 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9361 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9421 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9481 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9541 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9601 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9661 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9721 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9781 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9841 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9901 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9961 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    10021 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10081 ggcgatcggt gcgggcctct tcgctattac gccaggggag gcagagattg cagtaagctg
    10141 agatcgcagc actgcactcc agcctgggcg acagagtaag actctgtctc aaaaataaaa
    10201 taaataaatc aatcagatat tccaatcttt tcctttattt atttatttat tttctatttt
    10261 ggaaacacag tccttcctta ttccagaatt acacatatat tctatttttc tttatatgct
    10321 ccagtttttt ttagaccttc acctgaaatg tgtgtataca aaatctaggc cagtccagca
    10381 gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac
    10441 atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata
    10501 attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg
    10561 cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag
    10621 ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc
    10681 tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg
    10741 gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca
    10801 gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg
    10861 ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta
    10921 ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta
    10981 tatacagggt tcagtactat cgcatttcag gcctccacct gggtcttgga atgtgtcccc
    11041 cgaggggtga tgactacctc agttggatct ccacaggtca cagtgacaca agataaccaa
    11101 gacacctccc aaggctacca caatgggccg ccctccacgt gcacatggcc ggaggaactg
    11161 ccatgtcgga ggtgcaagca cacctgcgca tcagagtcct tggtgtggag ggagggacca
    11221 gcgcagcttc cagccatcca cctgatgaac agaacctagg gaaagcccca gttctactta
    11281 caccaggaaa ggc
//
