LOCUS       sequence-367736-       11407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human CD79B as YFP fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Sevdali E, Block V, Lataretu M, Li H, Smulski CR, Briem JS, Heitz Y,
            Fischer B, Ramirez NJ, Grimbacher B, Jack HM, Voll RE, Holzer M, 
            Schneider P, Eibel H
  TITLE     BAFFR activates PI3K/AKT signaling in human naive but not in 
            switched memory B cells through direct interactions with B cell 
            antigen receptors.
  JOURNAL   Cell Rep. 2022 Jun 28;39(13):111019. doi: 
            10.1016/j.celrep.2022.111019.
  PUBMED    35767961
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-06-28"; volume: "39(13):111019. doi"; pages: " 
            10.1016/j.celrep.2022.111019"
COMMENT     Sequence Label: pNL-CEF-CD79B-YFP
FEATURES             Location/Qualifiers
     source          1..11407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      1261..1396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2406..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2719..3901
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2950..3892
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3849..3869
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3923..3942
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3923..3941
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3967..3976
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4672..4681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4678..4680,4681..4683,4684..5397)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4723..4744)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4984..5003)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5331..5352
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5425..5443)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5426..5443)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5463..5480)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             5657..6290
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7931..7948)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8102..8690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8182..8201)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8861..9652,9653..9721))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9484..9503
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9722..9826)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9894..9912
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9950..9972)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10072..10091
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatat gactccgccc
     1261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1381 ggcttttttg gaggcctagg cttttgcaaa tgcataaaag aaaagggggg attggggggt
     1441 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1501 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctgt agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatctagtta actagatcta
     2401 gagtccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2701 attacccggc atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2761 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2821 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2881 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2941 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3001 ccttgcgtgc cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga
     3061 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     3121 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     3181 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     3241 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     3301 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     3361 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     3421 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     3481 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     3541 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     3601 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     3661 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     3721 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     3781 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     3841 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     3901 aggcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
     3961 gagctcgcca ccatggccag gctggcgttg tctcctgtgc ccagccactg gatggtggcg
     4021 ttgctgctgc tgctctcagc agctgagcca gtaccagcag ccagatcgga ggaccggtac
     4081 cggaatccca aaggtagtgc ttgttcgcgg atctggcaga gcccacgttt catagccagg
     4141 aaacggggct tcacggtgaa aatgcactgc tacatgaaca gcgcctccgg caatgtgagc
     4201 tggctctgga agcaggagat ggacgagaat ccccagcagc tgaagctgga aaagggccgc
     4261 atggaagagt cccagaacga atctctcgcc accctcacca tccaaggcat ccggtttgag
     4321 gacaatggca tctacttctg tcagcagaag tgcaacaaca cctcggaggt ctaccagggc
     4381 tgcggcacag agctgcgagt catgggattc agcaccttgg cacagctgaa gcagaggaac
     4441 acgctgaagg atggtatcat catgatccag acgctgctga tcatcctctt catcatcgtg
     4501 cctatcttcc tgctgctgga caaggatgac agcaaggctg gcatggagga agatcacacc
     4561 tacgagggcc tggacattga ccagacagcc acctatgagg acatagtgac gctgcggaca
     4621 ggggaagtga agtggtctgt aggtgagcac ccaggccagg aggacccggt cgccaccatg
     4681 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4741 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4801 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4861 gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     4921 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4981 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5041 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5101 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     5161 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5221 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5281 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5341 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     5401 ggccgcgact ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     5461 agcctcgact gtgccttcta gttgccagga gcaatcacaa gtagcaatac agcagctaac
     5521 aatgctgctt gtgcctggct agaagcacaa gaggaggaag aggtgggttt tccagtcaca
     5581 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5641 aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     5701 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     5761 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     5821 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     5881 ggaatggatg accctgagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     5941 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgaca tcgagcttgc
     6001 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     6061 tggcgagccc tcagatgctg catataagca gctgcttttt gcctgtactg ggtctctctg
     6121 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6181 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6241 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca cccaggaggt
     6301 agaggttgca gtgagccaag atcgcgccac tgcattccag cctgggcaag aaaacaagac
     6361 tgtctaaaat aataataata agttaagggt attaaatata tttatacatg gaggtcataa
     6421 aaatatatat atttgggctg ggcgcagtgg ctcacacctg cgcccggccc tttgggaggc
     6481 cgaggcaggt ggatcacctg agtttgggag ttccagacca gcctgaccaa catggagaaa
     6541 ccccttctct gtgtattttt agtagatttt attttatgtg tattttattc acaggtattt
     6601 ctggaaaact gaaactgttt ttcctctact ctgataccac aagaatcatc agcacagagg
     6661 aagacttctg tgatcaaatg tggtgggaga gggaggtttt caccagcaca tgagcagtca
     6721 gttctgccgc agactcggcg ggtgtccttc ggttcagttc caacaccgcc tgcctggaga
     6781 gaggtcagac cacagggtga gggctcagtc cccaagacat aaacacccaa gacataaaca
     6841 cccaacaggt ccaccccgcc tgctgcccag gcagagccga ttcaccaaga cgggaattag
     6901 gatagagaaa gagtaagtca cacagagccg gctgtgcggg agaacggagt tctattatga
     6961 ctcaaatcag tctccccaag cattcgggga tcagagtttt taaggataac ttagtgtgta
     7021 gggggccagt gagttggaga tgaaagcgta gggagtcgaa ggtgtccttt tgcgccgagt
     7081 cagttcctgg gtgggggcca caagatcgga tgagccagtt tatcaatccg ggggtgccag
     7141 ctgatccatg gagtgcaggg tctgcaaaat atctcaagca ctgattgatc ttaggtttta
     7201 caatagtgat gttaccccag gaacaatttg gggaaggtca gaatcttgta gcctgtagct
     7261 gcatgactcc taaaccataa tttctttttt gttttttttt tttttatttt tgagacaggg
     7321 tctcactctg tcacctaggc tggagtgcag tggtgcaatc acagctcact gcagcctcaa
     7381 cgtcgtaagc tcaagcgatc ctcccacctc agcctgcctg gtagctgaga ctacaagcga
     7441 cgccccagtt aatttttgta tttttggtag aggcagcgtt ttgccgtgtg gccctggctg
     7501 gtctcgaact cctgggctca agtgatccag cctcagcctc ccaaagtgct gggacaaccg
     7561 gggccagtca ctgcacctgg ccctaaacca taatttctaa tcttttggct aatttgttag
     7621 tcctacaaag gcagtctagt ccccaggcaa aaagggggtt tgtttcggga aagggctgtt
     7681 actgtctttg tttcaaacta taaactaagt tcctcctaaa cttagttcgg cctacaccca
     7741 ggaatgaaca aggagagctt ggaggttaga agcacgatgg aattggttag gtcagatctc
     7801 tttcactgtc tgagttataa ttttgcaatg gtggttcaaa gactgcccgc ttctgacacc
     7861 agtcgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7921 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7981 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8041 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8281 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8341 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8401 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8461 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8521 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8581 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8641 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8701 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8761 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8821 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8881 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8941 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9001 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9061 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9121 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9181 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9241 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9301 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9361 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9421 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9481 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9541 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9601 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9661 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9721 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9781 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9841 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     9901 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     9961 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
    10021 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    10081 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    10141 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    10201 cggtgcgggc ctcttcgcta ttacgccagg ggaggcagag attgcagtaa gctgagatcg
    10261 cagcactgca ctccagcctg ggcgacagag taagactctg tctcaaaaat aaaataaata
    10321 aatcaatcag atattccaat cttttccttt atttatttat ttattttcta ttttggaaac
    10381 acagtccttc cttattccag aattacacat atattctatt tttctttata tgctccagtt
    10441 ttttttagac cttcacctga aatgtgtgta tacaaaatct aggccagtcc agcagagcct
    10501 aaaggtaaaa aataaaataa taaaaaataa ataaaatcta gctcactcct tcacatcaaa
    10561 atggagatac agctgttagc attaaatacc aaataaccca tcttgtcctc aataatttta
    10621 agcgcctctc tccaccacat ctaactcctg tcaaaggcat gtgccccttc cgggcgctct
    10681 gctgtgctgc caaccaactg gcatgtggac tctgcagggt ccctaactgc caagccccac
    10741 agtgtgccct gaggctgccc cttccttcta gcggctgccc ccactcggct ttgctttccc
    10801 tagtttcagt tacttgcgtt cagccaaggt ctgaaactag gtgcgcacag agcggtaaga
    10861 ctgcgagaga aagagaccag ctttacaggg ggtttatcac agtgcaccct gacagtcgtc
    10921 agcctcacag ggggtttatc acattgcacc ctgacagtcg tcagcctcac agggggttta
    10981 tcacagtgca cccttacaat cattccattt gattcacaat ttttttagtc tctactgtgc
    11041 ctaacttgta agttaaattt gatcagaggt gtgttcccag aggggaaaac agtatataca
    11101 gggttcagta ctatcgcatt tcaggcctcc acctgggtct tggaatgtgt cccccgaggg
    11161 gtgatgacta cctcagttgg atctccacag gtcacagtga cacaagataa ccaagacacc
    11221 tcccaaggct accacaatgg gccgccctcc acgtgcacat ggccggagga actgccatgt
    11281 cggaggtgca agcacacctg cgcatcagag tccttggtgt ggagggaggg accagcgcag
    11341 cttccagcca tccacctgat gaacagaacc tagggaaagc cccagttcta cttacaccag
    11401 gaaaggc
//
