LOCUS       sequence-36775-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Egan DF, Shackelford DB, Mihaylova MM, Gelino S, Kohnz RA, Mair W, 
            Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, 
            Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ
  TITLE     Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase 
            connects energy sensing to mitophagy.
  JOURNAL   Science. 2011 Jan 28;331(6016):456-61. doi: 10.1126/science.1196371.
            Epub 2010 Dec 23.
  PUBMED    21205641
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1196371"; journalName: "Science"; date: 
            "2011-01-28- 28"; volume: "331"; issue: "6016"; pages: "456-61"
COMMENT     Sequence Label: pDONR221 - myc ULK1 4SA
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(443..472)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(481..580)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(596..613)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(596..612)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(605..627)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      676..762
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcaggatgtt ttggggcttc aggtcccggt ggatgatgcc cttgctgtgc agcagccgca
       61 tggcgccagc gatctgctgt aggaaaagcc tgacagtgtc ttcactcagt gtgcgcatag
      121 tgtgcaggta gtcagccagg tctccaccat tacaatactc catgaccagg tagacagaat
      181 tagccatttc ctggaagtca tacagcgcca cgatgttttc gtgctttagt tccttcagga
      241 ttttgatttc ctttcccagc agtgtttggg acttggcaag gttcttcttg ttaatgcatt
      301 tgacggccac ctccaggtcg tgcttctcgc ggtgtcgacc cttgaagacc accgcgaagg
      361 cgccgtgtcc aatcaggtcc ttgcgagaga actcgaactt gcccacggtc tcgacgccgc
      421 ctcggcccgg ctctattcct cccaagtctt cctcggagat tagcttttgt tccatggtga
      481 agcctgcttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca atttgttgca
      541 acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgagc ttaagactgg
      601 ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag
      661 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt
      721 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      781 aacgttgtga agc
//
