LOCUS       sequence-367785-        8627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for copper-inducible expression of firefly 
            luciferase driven by a minimal synthetic promoter with binding sites
            for CUP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: pEPCT-alpha-KN0009
FEATURES             Location/Qualifiers
     source          1..8627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             780..1853
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1919..2113
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(2349..2371)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2457..2597
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2512..2531
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2612..2629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2783..3371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2863..2882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3459..4274)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3459..4274)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4182..4201
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    4707..4731
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4937..4983
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    4999..5054
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5056..6705
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5122..5140)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             6769..6792
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6830..7004
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7024..7048
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             join(8349..8627,1..351)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg
       61 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta
      121 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc
      181 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc
      241 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct
      301 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag
      361 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca
      421 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact
      481 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga
      541 ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa
      601 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca
      661 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc
      721 ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg
      781 tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg
      841 tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc
      901 ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg
      961 cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg
     1021 ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt
     1081 tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg
     1141 ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg
     1201 tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag
     1261 acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag
     1321 ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc
     1381 acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg
     1441 gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca
     1501 tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg
     1561 tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc
     1621 gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg
     1681 aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg
     1741 ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc
     1801 tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg
     1861 agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaagga ctctttcctg
     1921 tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg
     1981 ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga
     2041 aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc
     2101 tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc
     2161 ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc
     2221 gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt
     2281 cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac
     2341 ggtgaaaacc tctgacacat gcagctcccg gtgacggtca cagcttgtct gtaagcggat
     2401 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     2461 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     2521 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     2581 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3181 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3421 actcacgtta agggattttg gtcatgcatt ctaggtgatt agaaaaactc atcgagcatc
     3481 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     3541 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     3601 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     3661 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     3721 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     3781 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag tcgaaatacg
     3841 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     3901 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     3961 gttttccctg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     4021 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     4081 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     4141 ccatacaatc ggtagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     4201 ccatataaat cagcatccat gttggaattt aatcgcggcc ttgagcaaga cgtttcccgt
     4261 tgaatatggc tcataacaga acttattatt tccttcctct tttctacagt atttaaagat
     4321 accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa
     4381 ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt
     4441 atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta
     4501 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     4561 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     4621 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     4681 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa cataacgaat
     4741 tcgtctcagg agagcgatgc gtcttttccg atgaaccgtt ccagcaaaaa agactaagcg
     4801 atgcgtcttt tccgctgaac cgttccagca aaaaagacta agcgatgcgt cttttccgct
     4861 gaaccgttcc agcaaaaaag actaggagag cgatgcgtct tttccgctga accgttccag
     4921 caaaaaagac tacttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     4981 ggatactgta tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta
     5041 ctatttacaa ttacaatgga ggacgccaaa aacataaaga aaggcccggc gccattctat
     5101 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     5161 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     5221 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     5281 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     5341 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     5401 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     5461 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     5521 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     5581 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     5641 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     5701 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     5761 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     5821 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagaact gtttctgagg
     5881 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     5941 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     6001 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     6061 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     6121 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     6181 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     6241 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     6301 ggatggctac attctggaga catagcttac tgggacgagg acgaacactt cttcatcgtt
     6361 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     6421 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct gcccgacgat
     6481 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     6541 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     6601 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     6661 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgggttc ggattataag
     6721 gaccatgacg gagactataa ggaccatgac ctcgacgctg cagcagcgga ttataaggac
     6781 gatgacgata agtgagcttc tctagctaga gtcgatcgac aagctcgagt ttctccataa
     6841 taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg
     6901 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     6961 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcgctgtc atgagacgaa
     7021 ttctgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     7081 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     7141 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     7201 cggcttctga cgttcagtgc agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt
     7261 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     7321 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     7381 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     7441 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     7501 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     7561 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     7621 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     7681 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     7741 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     7801 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     7861 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     7921 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     7981 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     8041 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     8101 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg
     8161 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     8221 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     8281 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     8341 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     8401 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     8461 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     8521 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     8581 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtcc
//
