LOCUS       sequence-367811-        9757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of Gal4:Phi-C31 
            driven by the 35s promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB UA_114 A
FEATURES             Location/Qualifiers
     source          1..9757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        658..1002
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     946..966
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1359..3173
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     CDS             3174..3194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3448..3472
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4773..5402
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5831..6904
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6970..7164
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7400..7422)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7508..7648
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7563..7582
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7663..7680)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7834..8422)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7914..7933)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8510..9325)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8510..9325)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9233..9252
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tggcaggata tattgtggtg taaacataac gaattcgtct caggagacta gagccaagct
       61 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
      121 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
      181 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct
      241 gatcaagacg atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt
      301 taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt
      361 tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag
      421 gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga
      481 gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat
      541 acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
      601 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
      661 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
      721 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
      781 taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc
      841 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      901 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
      961 cccttcctct atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca
     1021 attaccacaa caaaacaaac aacaaacaac attacaattt actattctag tcgaccatga
     1081 caaattctca agcgctttca caaccaattg cctcctctaa cgttcatgat aacttcatga
     1141 ataatgaaat cacggctagt aaaattgatg atggtaataa ttcaaaacca ctgtcacctg
     1201 gttggacgga ccaaactgcg tataacgcgt ttggaatcac tacagggatg tttaatacca
     1261 ctacaatgga tgatgtatat aactatctat tcgatgatga agatacccca ccaaaaccaa
     1321 aaaaagaggg tggcagtgga gggggcggaa gcgggggaat ggacacgtat gccggcgctt
     1381 atgatagaca atcaagagaa agagagaact cttctgccgc ctctccagct acacagagat
     1441 ctgccaacga ggataaagct gctgatttac aaagagaggt agagagagat gggggtaggt
     1501 ttagatttgt gggtcatttc tctgaggctc ctggtacatc agcctttggt actgctgaac
     1561 ggcctgaatt tgaaagaata ttaaatgagt gtcgggccgg tcgactaaat atgataatag
     1621 tttatgatgt atctaggttc agtagactca aagttatgga tgcaattccg atcgtgagcg
     1681 agctacttgc actcggtgtt actatcgtaa gtacacaaga aggtgtattc aggcagggta
     1741 atgtgatgga ccttatacac cttattatgc gactcgacgc tagccataaa gagtcatcac
     1801 tgaagagtgc taaaatcttg gacaccaaga acctgcaaag ggagcttgga ggctatgtag
     1861 gaggaaaagc tccgtatggt ttcgaactcg tgagtgagac aaaagagatt accagaaatg
     1921 gtaggatggt gaacgtggtt attaataagc tagctcatag cacaacccct ctcacagggc
     1981 cttttgagtt cgaacctgac gttattagat ggtggtggag agagatcaaa actcacaaac
     2041 atcttccatt taagccaggt agccaggctg ctatccaccc tggatctatc actgggcttt
     2101 gtaaaaggat ggatgctgac gcagtgccaa ctcgagggga aacaatcggt aagaagactg
     2161 catcttccgc ttgggatccc gctacagtta tgagaatcct tcgggatccg cgtattgctg
     2221 gattcgccgc agaagtcatt tataagaaaa aacctgatgg tactcctact acaaaaatcg
     2281 aaggttatag gatccaaagg gatcctatta ctttgcgtcc agttgaatta gactgtggac
     2341 cgatcatcga acctgccgag tggtacgagt tgcaggcctg gcttgatggt agagggcgtg
     2401 gaaagggttt atctcgggga caggcaattt tgtcagcaat ggataagctt tattgtgagt
     2461 gtggggctgt tatgacatca aaacgtggag aagagtcaat caaggattct tacaggtgca
     2521 gacgtcgtaa agtggtggac ccttctgctc caggacagca tgagggtaca tgcaacgttt
     2581 ctatggctgc tcttgataaa tttgtggccg aacgtatttt caacaagatt agacacgctg
     2641 aaggggatga ggaaacttta gctcttcttt gggaggctgc ccggcgtttt ggtaagctaa
     2701 cagaggcacc agaaaaaagt ggcgaaagag ctaatttggt agccgaaaga gcagacgcct
     2761 tgaatgcatt ggaagaactt tacgaagata gggctgccgg agcatatgat ggaccagttg
     2821 gtcggaagca tttcaggaag caacaagctg cactaacttt gagacagcaa ggcgcagagg
     2881 agcgactcgc agagcttgag gccgccgagg ccccaaagct tccactcgat caatggtttc
     2941 ctgaggatgc cgacgctgat cctacaggtc caaagagctg gtggggaaga gctagcgtag
     3001 acgataagag agtgtttgtg ggtctatttg ttgacaagat tgtagttact aagtccacta
     3061 cgggtcgagg acagggcact cccatcgaaa agagagccag tattacctgg gcaaagcctc
     3121 caaccgatga tgatgaagac gatgcacaag atggtaccga ggatgtggca gcaccaaaaa
     3181 aaaagaggaa ggtgtaagct tcggccatgc tagagtccgc aaaaatcacc agtctctctc
     3241 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     3301 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3361 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3421 gtgacctcgc tgtcatgaga cgaattctga caggatatat tggcgggtaa acctaagaga
     3481 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     3541 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     3601 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa
     3661 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     3721 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     3781 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     3841 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     3901 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     3961 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     4021 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     4081 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     4141 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     4201 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     4261 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     4321 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     4381 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     4441 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     4501 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     4561 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     4621 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     4681 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     4741 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     4801 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     4861 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     4921 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     4981 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     5041 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     5101 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     5161 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     5221 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     5281 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     5341 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     5401 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     5461 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     5521 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     5581 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     5641 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     5701 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     5761 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
     5821 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     5881 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     5941 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     6001 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     6061 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     6121 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     6181 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     6241 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     6301 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     6361 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     6421 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     6481 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     6541 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     6601 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     6661 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     6721 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     6781 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     6841 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     6901 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     6961 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     7021 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     7081 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     7141 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
     7201 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     7261 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     7321 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     7381 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc
     7441 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7501 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     7561 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     7621 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     7681 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7741 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7801 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7861 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7921 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7981 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8041 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8101 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8161 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8221 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8281 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8341 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8401 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8461 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact
     8521 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     8581 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     8641 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     8701 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     8761 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     8821 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     8881 gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     8941 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     9001 cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     9061 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     9121 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     9181 catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag
     9241 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag
     9301 acgtttcccg ttgaatatgg ctcataacag aacttattat ttccttcctc ttttctacag
     9361 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
     9421 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
     9481 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
     9541 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     9601 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     9661 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     9721 tgccgagctg ccggtcgggg agctgttggc tggctgg
//
