LOCUS       sequence-367829-       15853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module for copper inducible expression of AtrD11, HarFAR and EaDAct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 15853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: pEPKK-Omega-1SP0678
FEATURES             Location/Qualifiers
     source          1..15853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..296
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(532..554)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    640..780
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     695..714
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(795..812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(966..1554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1046..1065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1642..2433)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    3219..3243
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3273..3980)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(3988..4782)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4099..4118)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4709..4728
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    4784..4839
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        complement(4875..5054)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5220..5565
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      6604..6856
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7055..7101
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    7117..7172
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     polyA_signal    8189..8363
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        8559..8605
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      10258..10510
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     promoter        10707..10753
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      12211..12411
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    12433..12457
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             13758..14387
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(14816..15853,1..36)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
       61 attgccctag caggggaaaa aggtcgaaaa ggactctttc ctgtggatag cacgtacatt
      121 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
      181 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
      241 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
      301 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
      361 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
      421 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
      481 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
      541 catgcagctc ccggtgacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      601 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
      661 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
      721 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
      781 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      841 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      901 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1381 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     1441 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1501 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1561 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1621 ttggtcatgc attctaggtg attatttgcc gactaccttg gtgatctcgc ctttcacgta
     1681 gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag
     1741 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
     1801 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
     1861 ggacaacgta agcactacat ttcgctcatc accagcccag tcgggcggcg agttccatag
     1921 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
     1981 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
     2041 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca
     2101 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
     2161 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
     2221 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
     2281 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
     2341 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
     2401 agtcgatact tcggcgatca ccgcttccct cataatgttt aactttgttt tagggcgact
     2461 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     2521 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
     2581 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     2641 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     2701 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gtagcagcga
     2761 agtcgaggca tttctgtcct ggctggaaca gaacttatta tttccttcct cttttctaca
     2821 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
     2881 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
     2941 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct
     3001 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
     3061 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
     3121 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
     3181 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
     3241 aacataacgg atccggtctc aggagagcgg tcctgctgag cctcgacatg ttgtcgcaaa
     3301 attcgccctg gacccgccca acgatttgtc gtcactgtca aggtttgacc tgcacttcat
     3361 ttggggccca catacaccaa aaaaatgctg cataattctc ggggcagcaa gtcggttacc
     3421 cggccgccgt gctggaccgg gttgaatggt gcccgtaact ttcggtagag cggacggcca
     3481 atactcaact tcaaggaatc tcacccatgc gcgccggcgg ggaaccggag ttcccttcag
     3541 tgagcgttat tagttcgccg ctcggtgtgt cgtagatact agcccctggg gcacttttga
     3601 aatttgaata agatttatgt aatcagtctt ttaggtttga ccggttctgc cgcttttttt
     3661 aaaattggat ttgtaataat aaaacgcaat tgtttgttat tgtggcgctc tatcatagat
     3721 gtcgctataa acctattcag cacaatatat tgttttcatt ttaatattgt acatataagt
     3781 agtagggtac aatcagtaaa ttgaacggag aatattattc ataaaaatac gatagtaacg
     3841 ggtgatatat tcattagaat gaaccgaaac cggcggtaag gatctgagct acacatgctc
     3901 aggtttttta caacgtgcac aacagaattg aaagcaaata tcatgcgatc ataggcttct
     3961 cgcatatctc attaaagcag gacaagctca gaagaactcg tcaagaaggc gatagaaggc
     4021 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     4081 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     4141 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     4201 cggcaagcag gcatcgccgt gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt
     4261 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     4321 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     4381 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     4441 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     4501 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     4561 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tggagttcat tcagggcacc
     4621 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     4681 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     4741 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc attgtaattg taaatagtaa
     4801 ttgtaatgtt gtttgttgtt tgttgttgtt ggtaattgtt gtaaaaatac agtatgagag
     4861 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
     4921 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
     4981 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt
     5041 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
     5101 gtcataacgt gactccctta attctccgct ctccgtcagg aggtcaacat ggtggagcac
     5161 gacactctgg tctactccaa aaatgtcaaa gatacagtct cagaagatca aagggctatt
     5221 gagacttttc aacaaaggat aatttcggga aacctcctcg gattccattg cccagctatc
     5281 tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg ccatcattgc
     5341 gataaaggaa aggctatcat tcaagatctc tctgccgaca gtggtcccaa agatggaccc
     5401 ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa ccacgtctac aaagcaagtg
     5461 gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     5521 gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc gagtataaga
     5581 gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac aattacattt
     5641 acaattatcg atacaatgat ggtcgtaatt aacggggtca aatatgcctg tgaaacgtgt
     5701 atcaggggtc acagggcggc gcagtgtact cacactgatg gtccgctaca gatgatcaga
     5761 cgcaagggaa gaccatcgac cacatgtggc cattgtaaag agctgagaag aaccaagaac
     5821 ttcaacccat ccggtgggtg catgtgtgcc tctgcacgac ggccagctgt tggcagcaag
     5881 gaagatgaaa cacgatgtcg ttgtgatgag ggtgaacctt gtaaatgtca taccaagagg
     5941 aaaagcagcc ggaaatcaaa gggagggtca tgccacagaa gggcaaatga tgaagcagcg
     6001 catgtcaatg gtcttggtat tgcagatctg gacgttcttt tgggcctaaa tggtcgctcg
     6061 tcggatgtag acatgacaac cacattgccg agtttgaagc cacctctgca aaacggagaa
     6121 attaaggccg acagcattga caatcttgat ttggcttccc tcgatccgct tgagcaaagc
     6181 cctagtatat ctatggaacc tgttagtatc aatgaaacag gaagcgcata tacaactacg
     6241 aacacagcac taaacgatat tgacattcca ttctccatca atgagttgaa cgagctatac
     6301 aaacaagtat cttcgcataa ctcacattca cagccaatga caaattctca agcgctttca
     6361 caaccaattg cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt
     6421 aaaattgatg atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg
     6481 tataacgcgt ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat
     6541 aactatctat tcgatgatga agatacccca ccaaacccaa aaaaagagta ggcttgtcaa
     6601 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     6661 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     6721 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     6781 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     6841 tctatgttac tagatcgacg ctgtcaggag agcgatgcgt cttttccgat gaaccgttcc
     6901 agcaaaaaag actaagcgat gcgtcttttc cgctgaaccg ttccagcaaa aaagactaag
     6961 cgatgcgtct tttccgctga accgttccag caaaaaagac taggagagcg atgcgtcttt
     7021 tccgctgaac cgttccagca aaaaagacta cttcgcaaga cccttcctct atataaggaa
     7081 gttcatttca tttggagagg atactgtatt tttacaacaa ttaccaacaa caacaaacaa
     7141 caaacaacat tacaattact atttacaatt acaatggttc ctaataaggg ttcttctgat
     7201 gtcttgtccg aacattctga gccacagttc actaagctga tcgcccctca agccggacca
     7261 aggaagtata agatcgtgta ccgaaactta ctgactttcg gatattggca cctttctgct
     7321 gtttatggct tgtacctttg tttcacttgt gctaaatggg caacgatttt gtttgccttt
     7381 tttctttacg tgatagctga aattgggatt actggtggag ctcacagact ttgggcacat
     7441 agaacttaca aggctaaact gcccctagaa attcttcttc tcattatgaa ctccatagct
     7501 tttcaagata ctgcatttac ttgggccagg gaccatagac ttcaccacaa atactcagat
     7561 actgatgcag atcctcacaa cgcaacccgg ggtttttttt attcccacgt tgggtggttg
     7621 ttggtcaaaa agcatccaga ggttaaggct agaggaaagt acttgtctct cgatgactta
     7681 aagaataacc cgctattgaa gttccaaaaa aaatacgcca tactcgtaat tggaacctta
     7741 tgttttttga tgcctacgtt cgtcccggta tacttttggg gtgaaggcat ctctactgca
     7801 tggaacatca atcttttacg atatgtcatg aacttaaata tgactttcct agtcaactct
     7861 gctgcccata tatttgggaa caagccgtat gataaatcaa tagcttctgt gcaaaatata
     7921 tcagtttccc tggctacatt tggggagggt ttccataatt atcatcacac atatccctgg
     7981 gattatcgtg cagcagaact tggaaacaat agattgaata tgacaaccgc ctttattgat
     8041 ttcttcgcct ggataggttg ggcatatgat ctaaagtctg tgccacaaga ggctattgca
     8101 aaacggtgcg caaaaactgg agatggtact gatatgtggg gtaggaaacg ttgagcttct
     8161 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     8221 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     8281 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     8341 aatccagtac taaaatccag atcgctgtca ggagagcgat gcgtcttttc cgatgaaccg
     8401 ttccagcaaa aaagactaag cgatgcgtct tttccgctga accgttccag caaaaaagac
     8461 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaggag agcgatgcgt
     8521 cttttccgct gaaccgttcc agcaaaaaag actacttcgc aagacccttc ctctatataa
     8581 ggaagttcat ttcatttgga gaggatactg ttttagtttt attgcaacaa caacaacaaa
     8641 ttacaataac aacaaacaaa atacaaacaa caacaacaat gaatgaaatc gatgaaaaaa
     8701 atcaagctcc cgtacaacag gagtgtctca aggaaatgat acagaacggc cacgcaagga
     8761 ggatgggcag tgtagaggac ctctacgtag ccttaaacag acaaaacctg tatagaaact
     8821 tttgtacgta tggggaactt tccgactact gcacccgaga tcaactgaca ttagccctga
     8881 gggagatttg tctcaaaaat ccaacccttc tgcatatagt cttgccaacc cgttggccaa
     8941 accatgagaa ctattatcgt tcttccgaat attattcaag accccatcct gtccatgatt
     9001 atataagcgt actccaagag ttgaagcttt ctggggtagt gcttaatgaa caaccggaat
     9061 actctgcagt gatgaagcaa atccttgaag aatttaagaa ttcaaaaggt agctatacgg
     9121 caaaaatctt caagttgaca actactttga cgatacccta cttcgggcct accggtcctt
     9181 catggagatt gatctgtctc cccgaggaac atacagagaa gtggaggaag ttcatttttg
     9241 ttagcaacca ctgtatgtca gatggcaggt ccagtataca ttttttccat gatcttagag
     9301 atgagcttaa caatataaag acccctccaa agaagttaga ctatatcttt aagtatgaag
     9361 aggattacca gttattaagg aaactccctg agcctataga gaaagttata gactttaggc
     9421 caccgtattt attcatcccg aagagccttc ttagcggatt catctataac cacttacgat
     9481 ttagttccaa aggagtgtgt atgcgaatgg atgacgttga gaaaacggat gacgtcgtaa
     9541 cagaaataat aaatattagc cctacagaat ttcaagctat caaggccaat attaagagta
     9601 acatccaagg gaaatgtacg atcacgccct ttttgcatgt gtgttggttt gtatcccttc
     9661 acaagtgggg aaaatttttc aagcccctta acttcgaatg gctgactgac atttttatac
     9721 cagctgattg caggtcccaa ctcccagacg atgatgaaat gcgacagatg tatagatatg
     9781 gcgctaatgt agggtttata gacttcactc cctggataag cgagtttgat atgaacgaca
     9841 acaaagaaaa cttctggccc ttaattgaac actaccacga ggttatttct gaagccttga
     9901 ggaataagaa gcatcttcat ggccttggct ttaacattca gggattcgtt caaaagtatg
     9961 tcaatataga taaggttatg tgcgaccgtg ctattggaaa aagacgtggc ggtacgctct
    10021 tgagcaatgt gggtttgttt aatcaattgg aagagccgga cgccaaatat tctatatgcg
    10081 acttagcctt tggtcaattt cagggaagct ggcaccaagc cttcagtctt ggagtatgta
    10141 gcactaacgt caaaggaatg aacatagtgg ttgcatcaac taaaaacgtg gtaggatctc
    10201 aggagagctt agaggaatta tgttccattt acaaagctct gctgttaggg ccttaggctt
    10261 ggactcccat gttggcaaag gcaaccaaac aaacaatgaa tgatccgctc ctgcatatgg
    10321 ggcggtttga gtatttcaac tgccatttgg gctgaattgt agacatgctc ctgtcagaaa
    10381 ttccgtgatc ttactcaata ttcagtaatc tcggccaata tcctaaatgt gcgtggcttt
    10441 atctgtcttt gtattgtttc atcaattcat gtaacgtttg cttttcttat gaattttcaa
    10501 ataaattatc cgctgtcagg agagcgatgc gtcttttccg atgaaccgtt ccagcaaaaa
    10561 agactaagcg atgcgtcttt tccgctgaac cgttccagca aaaaagacta agcgatgcgt
    10621 cttttccgct gaaccgttcc agcaaaaaag actaggagag cgatgcgtct tttccgctga
    10681 accgttccag caaaaaagac tacttcgcaa gacccttcct ctatataagg aagttcattt
    10741 catttggaga ggatactgtt ttagttttat tgcaacaaca acaacaaatt acaataacaa
    10801 caaacaaaat acaaacaaca acaacaatgg ttgtgttgac tagcaaggag acaaagccta
    10861 gcgtcgctga attttacgct ggaaaaagtg tttttattac tgggggtact gggtttctcg
    10921 gtaaggtttt cattgagaaa ctcttgtatt cttgtccaga tatagaaaac atttacatgt
    10981 tgatcaggga aaaaaaaggt ttatcagtta gtgagaggat caagcagttt cttgacgacc
    11041 ctctgtttac caggttgaag gataagaggc ctgctgatct tgagaaaatc gtccttattc
    11101 ctggcgacat tactgctccc gacttgggta ttaacagtga gaacgaaaaa atgcttatag
    11161 aaaaggtgag tgttatcata cactcagcag ctaccgtcaa gttcaatgag ccattgccta
    11221 ccgcttggaa gatcaatgtc gagggtacac ggatgatgtt agcacttagc agacggatga
    11281 agagaataga ggttttcatc catatctcta ccgcttacac aaatacaaac agggaggtgg
    11341 ttgatgaaat tttgtaccct gcacctgctg acattgacca agttcaccag tacgtgaaag
    11401 atggtatctc tgaagaagat actgagaaga tcctcaatgg aaggcccaat acatatactt
    11461 tcaccaaggc tctgaccgag catttagttg ctgagaacca agcttatgtg ccaaccatta
    11521 tcgttaggcc gagtgttgtt gctgctataa aggacgagcc acttaaagga tggctgggca
    11581 attggtttgg cgctaccgga ttgacagttt ttaccgcaaa gggactaaac agggtcattt
    11641 atggtcattc ctcatacata gttgatctta ttcctgtaga ttacgttgct aatttagtta
    11701 tcgccgcagg agccaagtct tcaaaatcta cagaattgaa agtgtataat tgctgctctt
    11761 caagctgcaa cccagtgacc attgggacgt taatgagtat gttcgctgac gacgctataa
    11821 agcagaaaag ttatgcaatg ccgttaccgg gttggtacat atttaccaag tataagtggc
    11881 ttgtgctact gttgactttc ttatttcagg tcattcctgc ttatgttaca gatctctcta
    11941 ggcatctgat tggtaaatcc ccacgttata tcaagctcca atccctggtt aatcagacta
    12001 gatcatctat agatttcttt accaaccaca gctgggttat gaaggccgac cgggtgaggg
    12061 agttgtacgc atcactgtct cccgccgata aatacctatt cccgtgtgat cctaccgata
    12121 taaattggac tcattatata caggattatt gttggggtgt gaggcacttc ttggaaaaga
    12181 aatatcggtg agcttgatcc cccgtcgaca gctagctata tcatcaattt atgtattaca
    12241 cataatatcg cactcagtct ttcatctacg gcaatgtacc agctgatata atcagttatt
    12301 gaaatatttc tgaatttaaa cttgcatcaa taaatttatg tttttgcttg gactataata
    12361 cctgacttgt tattttatca ataaatattt aaactatatt tctttcaaga tcgctgtcat
    12421 gagaccggat cctgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
    12481 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
    12541 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
    12601 tagtgcagtc ggcttctgac gttcagtgca gccgtcatct gaaaacgaca tgtcgcacaa
    12661 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
    12721 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
    12781 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
    12841 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
    12901 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
    12961 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
    13021 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
    13081 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
    13141 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
    13201 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
    13261 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
    13321 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
    13381 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
    13441 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
    13501 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacctc
    13561 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
    13621 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
    13681 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
    13741 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
    13801 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
    13861 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
    13921 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
    13981 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
    14041 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
    14101 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
    14161 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
    14221 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
    14281 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
    14341 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
    14401 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
    14461 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
    14521 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
    14581 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
    14641 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
    14701 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
    14761 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
    14821 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
    14881 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
    14941 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
    15001 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
    15061 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
    15121 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
    15181 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
    15241 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
    15301 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
    15361 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
    15421 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
    15481 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
    15541 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
    15601 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
    15661 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
    15721 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
    15781 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
    15841 catgcgctac cgc
//
