LOCUS       sequence-367857-        9434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 loop assembly vector containing a transcriptional unit with 
            a Hygromycin resistance cassette under the control of the A. 
            agrestis EF1a promoter (pAaEF1a)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Frangedakis E, Waller M, Nishiyama T, Tsukaya H, Xu X, Yue Y, 
            Tjahjadi M, Gunadi A, Van Eck J, Li FW, Szovenyi P, Sakakibara K
  TITLE     An Agrobacterium-mediated stable transformation technique for the 
            hornwort model Anthoceros agrestis.
  JOURNAL   New Phytol. 2021 Jun 2. doi: 10.1111/nph.17524.
  PUBMED    34076270
REFERENCE   2  (bases 1 to 9434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2021 Jun 2. doi: 10.1111/nph.17524."
COMMENT     Sequence Label: L1-AA027
FEATURES             Location/Qualifiers
     source          1..9434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      353..547
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(783..805)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    891..1031
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     946..965
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1046..1063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1217..1805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1297..1316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1893..2708)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1893..2708)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2616..2635
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    3141..3165
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4725..5750
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     terminator      5771..6023
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    6070..6244
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6265..6289
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7590..8219
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(8648..9434,1..287)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
       61 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
      121 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
      181 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
      241 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
      301 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggactcttt cctgtggata
      361 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
      421 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
      481 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
      541 gtgcataact gtctggccag cgcacagccg aagtgctgca aaaagcgcct acccttcggt
      601 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
      661 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
      721 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
      781 aacctctgac acatgcagct cccggtgacg gtcacagctt gtctgtaagc ggatgccggg
      841 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
      901 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
      961 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     1021 accgcatcag gcgctcatcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1081 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1141 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1201 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1261 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1321 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1381 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1441 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1501 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1561 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1621 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     1681 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1741 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1801 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1861 gttaagggat tttggtcatg cattctaggt gattagaaaa actcatcgag catcaaatga
     1921 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     1981 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     2041 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     2101 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     2161 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     2221 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg
     2281 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     2341 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     2401 cctgggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     2461 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     2521 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     2581 aatcggtaga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     2641 aaatcagcat ccatgttgga atttaatcgc ggccttgagc aagacgtttc ccgttgaata
     2701 tggctcataa cagaacttat tatttccttc ctcttttcta cagtatttaa agatacccca
     2761 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     2821 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     2881 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     2941 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     3001 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     3061 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     3121 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacataac gaattgctct
     3181 tcaatgagga gcaccgtcca aacctggata gccgatatgt gagtgtttgg ttgcctgtca
     3241 tttctaaaaa gcgtatgtct cgtagcgtag atagcaagcg ctgtgagaaa ggtgtgctcc
     3301 tatagcatgg tagcatctgt cccatatctc gtccgcgaga agcctacatt tctcgttgcg
     3361 tattagagca tgaaaaacca acgtgaagta cgtgattcag accttgacca gttccagttc
     3421 tcttcaacaa aaaaacagtt cttcaattca tttgcttaca ttaaacaatt gtcacactct
     3481 tcttcccgga tgacagcacc aagacacttc ccctccacaa aaatctccga gtcatgtgga
     3541 ccggcctgga tgctcttaaa ggggttgcca ttgtagcggg gtgccttaac cacgacctct
     3601 tcgatacctg tctgaagcgg cgtgtgaaat cctccaggag cttgttcaaa tttacaagct
     3661 gcaatggatg taaactcatc caaagacatg acttgcgaga agccagaaac ggtcatcaac
     3721 actacgaaca cccctaaaca tggatggaga ggcaacatcc tgcatgtgtc tatgtcagag
     3781 gatgcaaaag tgaaagacaa aggagaagga ttgctcgtgc aatcactgca aagcaagatg
     3841 cgaatatccc taaaatccac agcaggaaaa gaaaggagcc tcaataagtc tctaaaatgg
     3901 gaagaaaaga agcacgggcc agcgagacgg aaatgaggcg atccgatgcg atggttccga
     3961 gaactgaagc ggtgcgtggg gggctagaaa tgaatgggga acgtggaggg caaacaaagg
     4021 ctgtacttag cggcaaatgc agatggcatg catgccaaga acagcagcag tcggcaccgc
     4081 cagtttagcg gagagcaaca ggcagcaaaa ggaagtggaa caggtttagg gccgacgcag
     4141 tcggcgcctg ccctaaaacg gcaaggccca cggcagcggt cgcgagggcg ttgcctgccg
     4201 gtcgtggaaa gccgtggggc tgtcaacgga acctcacgct cccagcctgc cctaagcggg
     4261 gaaaaagaag atccacattc tagggctttc catctcggct ctcagccgtc cgattaagaa
     4321 tcgcacggcc tagccccgcc agcctagccc tcggccgccc ccttgacagg ttaagagctg
     4381 ggcaagcgac tcagttgttc cttcgtcgct cgcccttctc acgagcagct cgcctgcccc
     4441 gccgccgcct tgcctgcctc gcgccttttg tctccaggtt cccttgccgc gccttctcct
     4501 tggcttgtgc ttctgctttg cttgtgcctc tgctgggctc tacttgtgtg gtgttgtgct
     4561 ttggcgccgc cgcctcgctg ctgctgtgga gcttggtgtg tgctggggtt tctggggttt
     4621 gcatgtgtca gggtttgtag atctgttctt cggggccgtc tttctaacgt ttcgtgtgcc
     4681 gttgtttctg gggggtttgg tgtggagctc gcagcttgca gctaatgaaa aagcctgaac
     4741 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     4801 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     4861 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     4921 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggag tttagcgaga
     4981 gcctgaccta ttgcatctcc cgccgttcac agggtgtcac gttgcaagac ctgcctgaaa
     5041 ccgaactgcc cgctgttcta caaccggtcg cggaggctat ggatgcgatc gctgcggccg
     5101 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     5161 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     5221 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     5281 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     5341 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     5401 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     5461 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccacgactc cgggcgtata
     5521 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     5581 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     5641 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     5701 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaagaaatag gcttgagctc
     5761 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     5821 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     5881 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     5941 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     6001 ggtgtcatct atgttactag atcgatccgt atcgatagcc tctagctaga gtcgatcgac
     6061 aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa ttagggttcc
     6121 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     6181 aaaatacttc tatcaataaa atttctaatt cctaaaatca aaatccagta ctaaaatcca
     6241 gatcgctagc atgaagagca attctgacag gatatattgg cgggtaaacc taagagaaaa
     6301 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     6361 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     6421 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtcat ctgaaaacga
     6481 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     6541 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     6601 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     6661 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     6721 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     6781 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     6841 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     6901 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     6961 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     7021 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     7081 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     7141 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     7201 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     7261 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     7321 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     7381 cccgcgcacc tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     7441 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     7501 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     7561 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     7621 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     7681 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     7741 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     7801 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     7861 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     7921 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     7981 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     8041 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     8101 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     8161 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     8221 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     8281 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     8341 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     8401 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     8461 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     8521 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     8581 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     8641 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     8701 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     8761 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     8821 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     8881 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     8941 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     9001 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     9061 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     9121 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     9181 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     9241 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     9301 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     9361 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     9421 cccggacgtg ctga
//
