LOCUS       sequence-367858-       11231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce HygR and eGFP under control of p35S into the 
            genome of the hornwort Anthoceros agrestis via Agrobacterium 
            mediated transformation. eGFP contains a cell membrane localization 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Frangedakis E, Waller M, Nishiyama T, Tsukaya H, Xu X, Yue Y, 
            Tjahjadi M, Gunadi A, Van Eck J, Li FW, Szovenyi P, Sakakibara K
  TITLE     An Agrobacterium-mediated stable transformation technique for the 
            hornwort model Anthoceros agrestis.
  JOURNAL   New Phytol. 2021 Jun 2. doi: 10.1111/nph.17524.
  PUBMED    34076270
REFERENCE   2  (bases 1 to 11231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2021 Jun 2. doi: 10.1111/nph.17524."
COMMENT     Sequence Label: L2-AA007
FEATURES             Location/Qualifiers
     source          1..11231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      195..389
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(625..647)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    733..873
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     788..807
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(888..905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1059..1647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1139..1158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1735..2526)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    3312..3336
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3855..4200
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4231..5256
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5291..5465
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        5874..6219
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(6230..6232,6233..6235,6236..6946)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6230..6232,6233..6235,6236..6946)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6275..6296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6536..6555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6883..6904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      7192..7444
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7491..7665
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7904..7928
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9229..9858
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(10287..11231,1..129)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
       61 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
      121 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
      181 aaaggactct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
      241 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
      301 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
      361 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagtgctg
      421 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
      481 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
      541 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
      601 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggtga cggtcacagc
      661 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      721 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
      781 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
      841 cacagatgcg taaggagaaa ataccgcatc aggcgctcat ccgcttcctc gctcactgac
      901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtgattattt
     1741 gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc
     1801 gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac
     1861 gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc
     1921 gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc
     1981 atcaccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa
     2041 tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac
     2101 gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc
     2161 gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc
     2221 tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag
     2281 aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg
     2341 cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt
     2401 caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg
     2461 gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc
     2521 cctcataatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
     2581 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     2641 agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     2701 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
     2761 cagcttacga accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg
     2821 tgtgcgtcac ccggcaacct tgggtagcag cgaagtcgag gcatttctgt cctggctgga
     2881 acagaactta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
     2941 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
     3001 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
     3061 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     3121 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     3181 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     3241 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     3301 tggctggctg gtggcaggat atattgtggt gtaaacataa cggatccggt ctcaggagaa
     3361 tgaggagaga ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag
     3421 gcttacgcag caagtctcat caagacgatc tacccgagca ataatctaca ggaaatcaaa
     3481 taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac
     3541 acagagaaag atatatttct caagatcaga agtactattc cagtatggac gattcaaggc
     3601 ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact
     3661 gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag
     3721 actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc
     3781 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagta
     3841 gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     3901 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     3961 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     4021 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaaaacgt tccaaccacg
     4081 tctacaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     4141 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca
     4201 cgggggactc tagaggatcc aacatcacca atgaaaaagc ctgaactcac cgcgacgtct
     4261 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     4321 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     4381 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     4441 gcgctcccga ttccggaagt gcttgacatt ggggagttta gcgagagcct gacctattgc
     4501 atctcccgcc gttcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     4561 gttctacaac cggtcgcgga ggctatggat gcgatcgctg cggccgatct tagccagacg
     4621 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     4681 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     4741 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     4801 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     4861 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     4921 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     4981 cggaggcatc cggagcttgc aggatcgcca cgactccggg cgtatatgct ccgcattggt
     5041 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     5101 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     5161 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     5221 cgacgcccca gcactcgtcc gagggcaaag aaataggctt ctctagctag agtcgatcga
     5281 caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga attagggttc
     5341 ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     5401 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc
     5461 agatcgctag caaggagtct gagcaggaca actcgcgtag tgagagttac atgttcgttg
     5521 ggttcttccg acacggacct gagttggcca acgtcccacc tgaggtctgt gccccggtga
     5581 tgagaagtgt gcatctcgtt cttgcagctc gtcagtactt tcagaatcat ggcgtgcatg
     5641 gtagaatgac ccttataacg gacttcgaca tggcaatcgc tatacaggag catggagtca
     5701 aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag
     5761 agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca
     5821 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact
     5881 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac
     5941 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
     6001 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     6061 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     6121 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     6181 tcctctatat aaggaagttc atttcatttg gagagaacac gggggactaa tggtgagcaa
     6241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     6301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     6361 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     6421 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     6481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     6541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     6601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     6661 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     6721 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     6781 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     6841 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     6901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctt cgggagctgc
     6961 ggcagctgcc gctgcggcag cggccgaatt caagcgcttg aaaatgagta cagccacttt
     7021 cgtagagatt attcttgcta tcatcttgcc tcctctcggc gtctttctca aatttggttg
     7081 caaggttgag ttttggatat gtttgatttt gacgctgttt ggttatcttc ccggaatcct
     7141 ttacgctctt tatatcatca ccttttgatg agcttgagct cgaatttccc cgatcgttca
     7201 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     7261 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     7321 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     7381 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     7441 gatcgatccg tatcgatagc ctctagctag agtcgatcga caagctcgag tttctccata
     7501 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     7561 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     7621 aatttctaat tcctaaaatc aaaatccagt actaaaatcc agatcgctac agaggagtct
     7681 gagcaggaca actcgcgtag tgagagttac atgttcgttg ggttcttccg acacggacct
     7741 gagttggcca acgtcccacc tgaggtctgt gccccggtga tgagaagtgt gcatctcgtt
     7801 cttgcagctc gtcagtactt tcagaatcat ggcgtgcatg gtagaatgac ccttataacg
     7861 gacttcgaca tggcaatcgc taggtatact tgagaccgga tcctgacagg atatattggc
     7921 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     7981 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     8041 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     8101 agccgtcatc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     8161 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     8221 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     8281 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     8341 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     8401 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     8461 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     8521 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     8581 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     8641 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     8701 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     8761 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     8821 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     8881 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     8941 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     9001 cgggtacgtg ttcgagccgc ccgcgcacct ctcaaccgtg cggctgcatg aaatcctggc
     9061 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     9121 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     9181 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     9241 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     9301 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     9361 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     9421 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     9481 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     9541 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     9601 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     9661 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     9721 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     9781 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     9841 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     9901 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     9961 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
    10021 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
    10081 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
    10141 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
    10201 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
    10261 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
    10321 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
    10381 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
    10441 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
    10501 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
    10561 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
    10621 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
    10681 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
    10741 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
    10801 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
    10861 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
    10921 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
    10981 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
    11041 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
    11101 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
    11161 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
    11221 tctgtttcac c
//
