LOCUS       sequence-367862-       12225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce eGFP under control of pAaEF1a into the genome of
            the hornwort Anthoceros agrestis via Agrobacterium mediated 
            transformation. eGFP contains a cell membrane localization tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Frangedakis E, Waller M, Nishiyama T, Tsukaya H, Xu X, Yue Y, 
            Tjahjadi M, Gunadi A, Van Eck J, Li FW, Szovenyi P, Sakakibara K
  TITLE     An Agrobacterium-mediated stable transformation technique for the 
            hornwort model Anthoceros agrestis.
  JOURNAL   New Phytol. 2021 Jun 2. doi: 10.1111/nph.17524.
  PUBMED    34076270
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2021 Jun 2. doi: 10.1111/nph.17524."
COMMENT     Sequence Label: L2-AA012
FEATURES             Location/Qualifiers
     source          1..12225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      196..390
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(626..648)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    734..874
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     789..808
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(889..906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1060..1648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1140..1159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1736..2527)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    3313..3337
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3856..4201
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4144..4164
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4232..5257
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5292..5466
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             join(7225..7227,7228..7230,7231..7941)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7225..7227,7228..7230,7231..7941)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7270..7291)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7531..7550)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7878..7899
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      8187..8439
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    8486..8660
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8899..8923
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10224..10853
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(11282..12225,1..130)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
       61 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
      121 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
      181 aaaaggactc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
      241 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
      301 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
      361 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagtgct
      421 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
      481 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
      541 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
      601 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag
      661 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      721 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
      781 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
      841 gcacagatgc gtaaggagaa aataccgcat caggcgctca tccgcttcct cgctcactga
      901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1501 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta ggtgattatt
     1741 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg
     1801 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga
     1861 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
     1921 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
     1981 catcaccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
     2041 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
     2101 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
     2161 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
     2221 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
     2281 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     2341 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct
     2401 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
     2461 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
     2521 ccctcataat gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
     2581 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
     2641 tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
     2701 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
     2761 acagcttacg aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg
     2821 gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg
     2881 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
     2941 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
     3001 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
     3061 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
     3121 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
     3181 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
     3241 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
     3301 ttggctggct ggtggcagga tatattgtgg tgtaaacata acggatccgg tctcaggaga
     3361 atgaggagag attagccttt tcaatttcag aaagaatgct aacccacaga tggttagaga
     3421 ggcttacgca gcaagtctca tcaagacgat ctacccgagc aataatctac aggaaatcaa
     3481 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
     3541 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
     3601 cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac
     3661 tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa
     3721 gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt
     3781 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcagt
     3841 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
     3901 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
     3961 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
     4021 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaaaacg ttccaaccac
     4081 gtctacaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     4141 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac
     4201 acgggggact ctagaggatc caacatcacc aatgaaaaag cctgaactca ccgcgacgtc
     4261 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     4321 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     4381 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     4441 cgcgctcccg attccggaag tgcttgacat tggggagttt agcgagagcc tgacctattg
     4501 catctcccgc cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     4561 tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc ttagccagac
     4621 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     4681 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     4741 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     4801 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     4861 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     4921 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     4981 gcggaggcat ccggagcttg caggatcgcc acgactccgg gcgtatatgc tccgcattgg
     5041 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     5101 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     5161 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     5221 ccgacgcccc agcactcgtc cgagggcaaa gaaataggct tctctagcta gagtcgatcg
     5281 acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt
     5341 cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     5401 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
     5461 cagatcgcta gcaaggagtc tgagcaggac aactcgcgta gtgagagtta catgttcgtt
     5521 gggttcttcc gacacggacc tgagttggcc aacgtcccac ctgaggtctg tgccccggtg
     5581 atgagaagtg tgcatctcgt tcttgcagct cgtcagtact ttcagaatca tggcgtgcat
     5641 ggtagaatga cccttataac ggacttcgac atggcaatcg ctatacagga gcaccgtcca
     5701 aacctggata gccgatatgt gagtgtttgg ttgcctgtca tttctaaaaa gcgtatgtct
     5761 cgtagcgtag atagcaagcg ctgtgagaaa ggtgtgctcc tatagcatgg tagcatctgt
     5821 cccatatctc gtccgcgaga agcctacatt tctcgttgcg tattagagca tgaaaaacca
     5881 acgtgaagta cgtgattcag accttgacca gttccagttc tcttcaacaa aaaaacagtt
     5941 cttcaattca tttgcttaca ttaaacaatt gtcacactct tcttcccgga tgacagcacc
     6001 aagacacttc ccctccacaa aaatctccga gtcatgtgga ccggcctgga tgctcttaaa
     6061 ggggttgcca ttgtagcggg gtgccttaac cacgacctct tcgatacctg tctgaagcgg
     6121 cgtgtgaaat cctccaggag cttgttcaaa tttacaagct gcaatggatg taaactcatc
     6181 caaagacatg acttgcgaga agccagaaac ggtcatcaac actacgaaca cccctaaaca
     6241 tggatggaga ggcaacatcc tgcatgtgtc tatgtcagag gatgcaaaag tgaaagacaa
     6301 aggagaagga ttgctcgtgc aatcactgca aagcaagatg cgaatatccc taaaatccac
     6361 agcaggaaaa gaaaggagcc tcaataagtc tctaaaatgg gaagaaaaga agcacgggcc
     6421 agcgagacgg aaatgaggcg atccgatgcg atggttccga gaactgaagc ggtgcgtggg
     6481 gggctagaaa tgaatgggga acgtggaggg caaacaaagg ctgtacttag cggcaaatgc
     6541 agatggcatg catgccaaga acagcagcag tcggcaccgc cagtttagcg gagagcaaca
     6601 ggcagcaaaa ggaagtggaa caggtttagg gccgacgcag tcggcgcctg ccctaaaacg
     6661 gcaaggccca cggcagcggt cgcgagggcg ttgcctgccg gtcgtggaaa gccgtggggc
     6721 tgtcaacgga acctcacgct cccagcctgc cctaagcggg gaaaaagaag atccacattc
     6781 tagggctttc catctcggct ctcagccgtc cgattaagaa tcgcacggcc tagccccgcc
     6841 agcctagccc tcggccgccc ccttgacagg ttaagagctg ggcaagcgac tcagttgttc
     6901 cttcgtcgct cgcccttctc acgagcagct cgcctgcccc gccgccgcct tgcctgcctc
     6961 gcgccttttg tctccaggtt cccttgccgc gccttctcct tggcttgtgc ttctgctttg
     7021 cttgtgcctc tgctgggctc tacttgtgtg gtgttgtgct ttggcgccgc cgcctcgctg
     7081 ctgctgtgga gcttggtgtg tgctggggtt tctggggttt gcatgtgtca gggtttgtag
     7141 atctgttctt cggggccgtc tttctaacgt ttcgtgtgcc gttgtttctg gggggtttgg
     7201 tgtggagctc gcagcttgca gctaatggtg agcaagggcg aggagctgtt caccggggtg
     7261 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     7321 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     7381 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     7441 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     7501 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     7561 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     7621 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     7681 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     7741 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     7801 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     7861 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     7921 ggcatggacg agctgtacaa ggcttcggga gctgcggcag ctgccgctgc ggcagcggcc
     7981 gaattcaagc gcttgaaaat gagtacagcc actttcgtag agattattct tgctatcatc
     8041 ttgcctcctc tcggcgtctt tctcaaattt ggttgcaagg ttgagttttg gatatgtttg
     8101 attttgacgc tgtttggtta tcttcccgga atcctttacg ctctttatat catcaccttt
     8161 tgatgagctt gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     8221 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     8281 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     8341 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     8401 ataaattatc gcgcgcggtg tcatctatgt tactagatcg atccgtatcg atagcctcta
     8461 gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
     8521 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
     8581 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaatcaaaat
     8641 ccagtactaa aatccagatc gctacagagg agtctgagca ggacaactcg cgtagtgaga
     8701 gttacatgtt cgttgggttc ttccgacacg gacctgagtt ggccaacgtc ccacctgagg
     8761 tctgtgcccc ggtgatgaga agtgtgcatc tcgttcttgc agctcgtcag tactttcaga
     8821 atcatggcgt gcatggtaga atgaccctta taacggactt cgacatggca atcgctaggt
     8881 atacttgaga ccagatcctg acaggatata ttggcgggta aacctaagag aaaagagcgt
     8941 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     9001 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     9061 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcatctgaaa acgacatgtc
     9121 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     9181 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     9241 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     9301 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     9361 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     9421 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     9481 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     9541 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     9601 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     9661 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     9721 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     9781 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     9841 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     9901 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     9961 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
    10021 cacctctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
    10081 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
    10141 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
    10201 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
    10261 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
    10321 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
    10381 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
    10441 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
    10501 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
    10561 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
    10621 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
    10681 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
    10741 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
    10801 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
    10861 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
    10921 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
    10981 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
    11041 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
    11101 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
    11161 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
    11221 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
    11281 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
    11341 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
    11401 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
    11461 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
    11521 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
    11581 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
    11641 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
    11701 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
    11761 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
    11821 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
    11881 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
    11941 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
    12001 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
    12061 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
    12121 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
    12181 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcac
//
