LOCUS       sequence-367863-        3430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 loop assembly vector containing the Anthoceros agrestis 
            Tip1;1 promoter (pAaTip1;1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Frangedakis E, Waller M, Nishiyama T, Tsukaya H, Xu X, Yue Y, 
            Tjahjadi M, Gunadi A, Van Eck J, Li FW, Szovenyi P, Sakakibara K
  TITLE     An Agrobacterium-mediated stable transformation technique for the 
            hornwort model Anthoceros agrestis.
  JOURNAL   New Phytol. 2021 Jun 2. doi: 10.1111/nph.17524.
  PUBMED    34076270
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2021 Jun 2. doi: 10.1111/nph.17524."
COMMENT     Sequence Label: L0-AA018
FEATURES             Location/Qualifiers
     source          1..3430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..66
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(90..107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(261..849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(341..360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1032..1126)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1147..1806)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1721..1740
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1968..1986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1990..2033)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 ccctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
       61 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
      121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      241 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
      301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1021 tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1081 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1141 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1201 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1261 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1321 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1381 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1441 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1501 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1561 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     1621 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     1681 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     1741 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     1801 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     1861 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc
     1921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1981 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     2041 cggtctctgg agcacctgga agcatttcca cacagcatct acaccactac agggctacat
     2101 gctccatagt gtagagcaaa ccctagtgca gctcagatat tagataatgc tgtccttccc
     2161 agaaagcaaa aggaatcgac aactctggaa ggcagacagc accagttctg tgcagcttca
     2221 tcccgagctg atgctgtcct tccgagaaag caaaggaatc gacactccat ggccgaccgc
     2281 acaaacacac catagatgct gacctatctg gatatgcaac aaccacaaca cttgcgtctc
     2341 caccgctcca agtccagcct ccaggagcca ggactcacgg ccgggtcgag tgggcccagc
     2401 ctggtcccgt ggcgccgggg cccgggcccg gctcccccca ctccccggcg cgattcttac
     2461 gcggcggacg gataggccca cgaccgtcct acgtggcggg ccccacgtaa ggacagcggt
     2521 gcctaggctg caccaaatgc cgcgccaccg tccatgtcgt cagaatcggt cagatattag
     2581 cccctatccc ttcacacgcc agcgccacgc cacgccacgc tgctgacgcc acccccctct
     2641 caccatcatc atagcctttc cagatatctc cacgctcctg cattacctgt acatcatttt
     2701 aatatgtgca tacatatgta taatcaatca gtaaaggcaa ggcaggctgg atccccagta
     2761 gagcccatca atgccttgaa tctcgtgttt cccgtcaggt cctttcattc attccaagtc
     2821 tccttctcat acatacatcc ctccgtgatt taaactttat taaataccta catagcaatg
     2881 gaataaatct ataatgtgga agagactcca aggtgtgact gaggttccac atgtctgaaa
     2941 gaaggagcat caaagtatct accttccctt ccatttggtt gtattccctt catcccctaa
     3001 acccaatgca agtagcaaaa gagggacacg agaaggtagt agtgaaggta ttgcattatt
     3061 gatttgtgaa agcgtttgat gaaacacaaa gggcacacaa gaagcgaatg aatggatcca
     3121 cacatggagg ggcaggcagc tcgaaaaaca gcagaggatg cacatggccc agagcggata
     3181 ggaaagcagg ccagcctcac tggacagtgg acagccaggt gcagcgcacc ctggcatatt
     3241 cgaaccggaa gggctgacgt gggctccatt atttggggtg ctgagttggc aaacacgcgc
     3301 cgggaagctc tcaaaaccgg catctccatc tcattccaac gccgcagtcc cattgctcac
     3361 caaccccggc tcctttcccc gctctgctcc tcggtttcac gctccgcgcg agctctgctg
     3421 caatgtgaga
//
