LOCUS       sequence-367893-        3593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 loop assembly vector containing the Anthoceros agrestis EF1a
            promoter (pAaEF1a)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Frangedakis E, Waller M, Nishiyama T, Tsukaya H, Xu X, Yue Y, 
            Tjahjadi M, Gunadi A, Van Eck J, Li FW, Szovenyi P, Sakakibara K
  TITLE     An Agrobacterium-mediated stable transformation technique for the 
            hornwort model Anthoceros agrestis.
  JOURNAL   New Phytol. 2021 Jun 2. doi: 10.1111/nph.17524.
  PUBMED    34076270
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2021 Jun 2. doi: 10.1111/nph.17524."
COMMENT     Sequence Label: L0-AA008
FEATURES             Location/Qualifiers
     source          1..3593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      152..740
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     641..660
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     894..911
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(935..992)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      2561..2604
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2608..2626)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2788..3447
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2854..2873)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3468..3562
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
       61 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
      301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
      361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
      421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
      481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
      541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      721 gggggcggag cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      841 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      901 cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc
      961 agggtggtga caccttgccc ttttttgccg gactgcaggg tctctggagc accgtccaaa
     1021 cctggatagc cgatatgtga gtgtttggtt gcctgtcatt tctaaaaagc gtatgtctcg
     1081 tagcgtagat agcaagcgct gtgagaaagg tgtgctccta tagcatggta gcatctgtcc
     1141 catatctcgt ccgcgagaag cctacatttc tcgttgcgta ttagagcatg aaaaaccaac
     1201 gtgaagtacg tgattcagac cttgaccagt tccagttctc ttcaacaaaa aaacagttct
     1261 tcaattcatt tgcttacatt aaacaattgt cacactcttc ttcccggatg acagcaccaa
     1321 gacacttccc ctccacaaaa atctccgagt catgtggacc ggcctggatg ctcttaaagg
     1381 ggttgccatt gtagcggggt gccttaacca cgacctcttc gatacctgtc tgaagcggcg
     1441 tgtgaaatcc tccaggagct tgttcaaatt tacaagctgc aatggatgta aactcatcca
     1501 aagacatgac ttgcgagaag ccagaaacgg tcatcaacac tacgaacacc cctaaacatg
     1561 gatggagagg caacatcctg catgtgtcta tgtcagagga tgcaaaagtg aaagacaaag
     1621 gagaaggatt gctcgtgcaa tcactgcaaa gcaagatgcg aatatcccta aaatccacag
     1681 caggaaaaga aaggagcctc aataagtctc taaaatggga agaaaagaag cacgggccag
     1741 cgagacggaa atgaggcgat ccgatgcgat ggttccgaga actgaagcgg tgcgtggggg
     1801 gctagaaatg aatggggaac gtggagggca aacaaaggct gtacttagcg gcaaatgcag
     1861 atggcatgca tgccaagaac agcagcagtc ggcaccgcca gtttagcgga gagcaacagg
     1921 cagcaaaagg aagtggaaca ggtttagggc cgacgcagtc ggcgcctgcc ctaaaacggc
     1981 aaggcccacg gcagcggtcg cgagggcgtt gcctgccggt cgtggaaagc cgtggggctg
     2041 tcaacggaac ctcacgctcc cagcctgccc taagcgggga aaaagaagat ccacattcta
     2101 gggctttcca tctcggctct cagccgtccg attaagaatc gcacggccta gccccgccag
     2161 cctagccctc ggccgccccc ttgacaggtt aagagctggg caagcgactc agttgttcct
     2221 tcgtcgctcg cccttctcac gagcagctcg cctgccccgc cgccgccttg cctgcctcgc
     2281 gccttttgtc tccaggttcc cttgccgcgc cttctccttg gcttgtgctt ctgctttgct
     2341 tgtgcctctg ctgggctcta cttgtgtggt gttgtgcttt ggcgccgccg cctcgctgct
     2401 gctgtggagc ttggtgtgtg ctggggtttc tggggtttgc atgtgtcagg gtttgtagat
     2461 ctgttcttcg gggccgtctt tctaacgttt cgtgtgccgt tgtttctggg gggtttggtg
     2521 tggagctcgc agcttgcagc taatgtgaga ccgaattcca gaaatcatcc ttagcgaaag
     2581 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
     2641 tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga
     2701 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     2761 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     2821 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     2881 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     2941 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     3001 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     3061 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     3121 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     3181 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     3241 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     3301 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     3361 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     3421 tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac tcctgttgat
     3481 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
     3541 ggcgtttttt attggtgaga atccaagcct cgagctgtca gaccaagttt act
//
