LOCUS       sequence-367905-       13219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector for production of lentivirus. Expresses 
            EGFP-P2A-HAUS6 (WT full length).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   David AF, Roudot P, Legant WR, Betzig E, Danuser G, Gerlich DW
  TITLE     Augmin accumulation on long-lived microtubules drives amplification 
            and kinetochore-directed growth.
  JOURNAL   J Cell Biol. 2019 Jul 1;218(7):2150-2168. doi: 
            10.1083/jcb.201805044. Epub 2019 May 21.
  PUBMED    31113824
REFERENCE   2  (bases 1 to 13219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201805044"; journalName: "J Cell Biol"; date: 
            "2019-07-1- 1"; volume: "218"; issue: "7"; pages: "2150-2168"
COMMENT     Sequence Label: Lenti_EGFP-P2A-HAUS6_IRES_Blast
FEATURES             Location/Qualifiers
     source          1..13219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2876..2885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2882..2884,2885..2887,2888..3598)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2882..2884,2885..2887,2888..3598)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2927..2948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3188..3207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3535..3556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3608..3664
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     intron          6602..6831
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6814..6833
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6889..7460
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7054..7071)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7281..7300
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7493..7891
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7911..8499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7964..7984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8382..8393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8502..8518)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8503..8519)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9024..9204
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9230..9247)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9236..9460
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9506..9934
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9593..9612)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9803..9824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9943..9963)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9948..10277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10128..10263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10190..10209
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10325..10372
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10391..10765
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10895..11016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10932..10951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10986..11005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11065..11081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11065..11081)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11078..11100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11089..11105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11113..11119,11120..11137,11138..11143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11158..11179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11296..11313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11467..12055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11547..11566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12226..13017,13018..13086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12849..12868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13087..13191)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggagaattct ctagagccac
     2881 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2941 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3001 tggtaagctt accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3061 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3121 gcagcacgac tttttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3181 ctttaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3241 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3301 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3361 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3421 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3481 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3541 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     3601 ttccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     3661 accgaccggt atgagctcgg cctcggtcac cgctttcgag aaggagcatc tctggatgta
     3721 tctgcaggcg ctcggcttcg agccaggccc ggcaaccatt gcctgcggaa agatcgtgtc
     3781 gcacacgcac ctcggagtga acatgtttga caagctgaac cgtgatgcct ttcatataat
     3841 ttcttatttt ttgtttcaag ttctggacca gtctctcacc aaagaagttt tcaaattttg
     3901 ttggccccca tttgaccaaa aaagtgacac tgaattccga aaacattgct gtgaatggat
     3961 aaaaaggatt tctggtgaat gtggaagcag ctttcctcaa gttgttggtt cactatttct
     4021 ttctcctggt ggtcctaagt ttattcatct gatgtatcat tttgcaagat ttgttgcaat
     4081 gaaatatatt aaatccaatt ctaaaaattc ttctcatcat tttgtagaga catttaacat
     4141 aaaaccacag gacttgcaca aatgcattgc cagatgccat ttcgcacgta gcagattttt
     4201 acaaattttg caaagacaag attgtgttac ccaaaaatat caggaaaatg cacaattatc
     4261 agttaagcag gtacgaaact tgagatctga atgtatagga ttggaaaacc aaataaagaa
     4321 aatggaaccc tatgatgacc acagtaatat ggaagaaaaa attcaaaagg ttcggtcttt
     4381 gtgggcttca gtgaatgaaa cgctcatgtt tttggaaaaa gagagagaag ttgttagttc
     4441 ggtccttagt cttgttaacc aatatgcttt agatggaact aatgttgcta ttaatattcc
     4501 aaggctctta cttgacaaaa ttgagaaaca aatgtttcag ttgcacatag gaaatgttta
     4561 tgaggctgga aaactgaacc tcttaacagt tattcagtta ttaaatgaag tcttgaaggt
     4621 gatgaaatat gaacgttgtc aggctgatca agcaagattg acggtagacc ttcactacct
     4681 tgaaaaagag accaaatttc agaaggaaag attatcagat ctgaaacata tgaggtatag
     4741 aataaaagat gatctcacaa ctataagaca ttctgttgtt gaaaagcaag gagaatggca
     4801 taaaaagtgg aaagaatttc ttggtttgtc tcctttcagt ctaattaaag gttggactcc
     4861 atctgtagat cttttaccac caatgtctcc cctttcgttt gatcctgcct cagaagaagt
     4921 gtatgcaaag agtattcttt gtcagtatcc tgcttcactt ccagatgcac ataagcaaca
     4981 taaccaagaa aatggttgca gaggagacag tgataccttg ggagcgctac atgatctagc
     5041 caacagccct gcctctttct tgtcgcagtc agtttcatca tcagatagaa acagtgttac
     5101 agtacttgaa aaggacacaa agatgggaac tcccaaagaa aaaaatgaag caatttctaa
     5161 gaaaatacca gaatttgaag tggaaaattc tccattatca gatgttgcaa agaatacaga
     5221 gagtagtgca tttggagggt ctttgccagc taaaaaaagt gatccatttc aaaaagagca
     5281 agatcatctg gtagaagagg ttgccagagc agttttatct gattcaccac agctctctga
     5341 aggaaaagaa ataaaattag aggaactaat tgactctctg ggttctaacc ccttcttaac
     5401 aaggaatcag attccccgta ctccagaaaa cttgataact gaaattagga gctcatggag
     5461 aaaagctatt gaaatggaag aaaacagaac taaagaacca attcaaatgg atgttgaaca
     5521 tagagaagta ttgccagaat cattacctgt gttgcacaat caaagagaat ttagcatggc
     5581 tgattttctc ttagaaacca ctgtatcaga ttttggccag tctcatttga ctgaagagaa
     5641 agttatttca gattgcgagt gtgtgcctca gaaacatgtg ctgaccagtc acatagatga
     5701 accaccaaca caaaatcagt cagatttgtt aaataagaaa gtaatttgca agcaagattt
     5761 ggaatgttta gccttcacca agctttcaga aactagccga atggagacat tctcccctgc
     5821 tgtcggcaat aggatagatg tgatgggtgg cagtgaagag gagtttatga aaatattgga
     5881 ccacttagaa gtttcttgta acaaaccttc cacaaataaa actatgttgt ggaattcttt
     5941 tcagatatca attggaatta gttctaagag ttttaaagat aatgattttg gcatattaca
     6001 cgaaactctc ccggaagaag ttggtcatct aagttttaat agttccagta gttcagaggc
     6061 caattttaaa ctggagccaa atagtcctat gcatggtggc actcttctag aagatgttgt
     6121 gggagggaga cagactactc cagaatcaga ctttaattta caggctcttc gcagtagata
     6181 cgaggctctg aagaaatctc tttccaagaa aagggaagaa tcttacctct cgaattccca
     6241 aacacccgaa agacacaaac cagaattgag ccctactccc caaaatgtac aaacagatga
     6301 tacgcttaac tttttggaca cctgtgattt gcatactgag catataaagc catctttacg
     6361 cacgtccatc ggtgaaagaa aacggtctct ttcaccacta attaagtttt ctccagtgga
     6421 acaaagattg agaaccacaa tagcatgtag tcttggagaa ctacctaatt taaaggaaga
     6481 agacattttg aataagagcc ttgatgcaaa agaaccaccg tctgacttga caagatgagg
     6541 atccgaattc cgatccagtg tgctggaatt aattcgctgt ctgcgagggc cagctgttgg
     6601 ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa
     6661 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc
     6721 atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc
     6781 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact
     6841 cccaggtcca actgcaggtc gagcatgcat ctagggcggc caattccgcc cctctccccc
     6901 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     6961 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     7021 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     7081 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     7141 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     7201 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     7261 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     7321 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     7381 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     7441 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatggccaa
     7501 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     7561 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     7621 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     7681 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     7741 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     7801 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     7861 gctgccctct ggttatgtgt gggagggcta acgatatcaa gcttatcggt aatcaacctc
     7921 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7981 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     8041 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     8101 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     8161 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     8221 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     8281 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     8341 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     8401 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     8461 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     8521 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     8581 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     8641 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8701 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     8761 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     8821 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     8881 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     8941 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     9001 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9061 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9121 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9181 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     9241 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     9301 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     9361 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     9421 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     9481 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9541 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9601 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9661 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9721 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9781 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9841 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9901 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     9961 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10021 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10081 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10141 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10201 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10261 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    10321 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
    10381 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    10441 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    10501 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    10561 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    10621 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    10681 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    10741 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    10801 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    10861 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    10921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    11041 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    11101 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    11161 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    11221 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    11281 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    11341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    11401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11881 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    12001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    12061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    12121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    12181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    12241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    12301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    12361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    12421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    12481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    12541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    12601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    12841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    12961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    13021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    13081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    13141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    13201 ccgaaaagtg ccacctgac
//
