LOCUS       sequence-367930-       12650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector for production of lentivirus. Control plasmid for 
            rescue experiment of HAUS6 depletion phenotype, containing a 
            nonsense mutation and a truncation in HAUS6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12650)
  AUTHORS   David AF, Roudot P, Legant WR, Betzig E, Danuser G, Gerlich DW
  TITLE     Augmin accumulation on long-lived microtubules drives amplification 
            and kinetochore-directed growth.
  JOURNAL   J Cell Biol. 2019 Jul 1;218(7):2150-2168. doi: 
            10.1083/jcb.201805044. Epub 2019 May 21.
  PUBMED    31113824
REFERENCE   2  (bases 1 to 12650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201805044"; journalName: "J Cell Biol"; date: 
            "2019-07-1- 1"; volume: "218"; issue: "7"; pages: "2150-2168"
COMMENT     Sequence Label: Lenti_EGFP-P2A_truncHaus6_IRES_Blast
FEATURES             Location/Qualifiers
     source          1..12650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2876..2885
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2882..2884,2885..2887,2888..3598)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2882..2884,2885..2887,2888..3598)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2927..2948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3188..3207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3535..3556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3608..3664
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     intron          6033..6262
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6245..6264
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6320..6891
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6485..6502)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6712..6731
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6924..7322
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7342..7930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7395..7415)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7813..7824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7933..7949)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7934..7950)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8455..8635
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8661..8678)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8667..8891
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8937..9365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9024..9043)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9234..9255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9374..9394)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9379..9708
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9559..9694
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9621..9640
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9756..9803
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9822..10196
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10326..10447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10363..10382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10417..10436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10496..10512)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10496..10512)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10509..10531)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10520..10536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10544..10550,10551..10568,10569..10574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10589..10610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10727..10744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10898..11486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10978..10997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11657..12448,12449..12517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12280..12299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12518..12622)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggagaattct ctagagccac
     2881 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2941 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3001 tggtaagctt accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3061 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3121 gcagcacgac tttttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3181 ctttaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3241 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3301 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3361 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3421 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3481 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3541 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     3601 ttccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
     3661 accgaccggt atgagctagg cctcggtcac cgctttcgag aaggagcatc tctggatgta
     3721 tctgcaggcg ctcggcttcg agccaggccc ggcaaccatt gcctgcggaa agatcgtgtc
     3781 gcacacgcac ctcggagtga acatgtttga caagctgaac cgtgatgcct ttcatataat
     3841 ttcttatttt ttgtttcaag ttctggacca gtctctcacc aaagaagttt tcaaattttg
     3901 ttggccccca tttgaccaaa aaagtgacac tgaattccga aaacattgct gtgaatggat
     3961 aaaaaggatt tctggtgaat gtggaagcag ctttcctcaa gttgttggtt cactatttct
     4021 ttctcctggt ggtcctaagt ttattcatct gatgtatcat tttgcaagat ttgttgcaat
     4081 gaaatatatt aaatccaatt ctaaaaattc ttctcatcat tttgtagaga catttaacat
     4141 aaaaccacag gacttgcaca aatgcattgc cagatgccat ttcgcacgta gcagattttt
     4201 acaaattttg caaagacaag attgtgttac ccaaaaatat caggaaaatg cacaattatc
     4261 agttaagcag gtacgaaact tgagatctga atgtatagga ttggaaaacc aaataaagaa
     4321 aatggaaccc tatgatgacc acagtaatat ggaagaaaaa attcaaaagg ttcggtcttt
     4381 gtgggcttca gtgaatgaaa cgctcatgtt tttggaaaaa gagagagaag ttgttagttc
     4441 ggtccttagt cttgttgcta catgatctag ccaacagccc tgcctctttc ttgtcgcagt
     4501 cagtttcatc atcagataga aacagtgtta cagtacttga aaaggacaca aagatgggaa
     4561 ctcccaaaga aaaaaatgaa gcaatttcta agaaaatacc agaatttgaa gtggaaaatt
     4621 ctccattatc agatgttgca aagaatacag agagtagtgc atttggaggg tctttgccag
     4681 ctaaaaaaag tgatccattt caaaaagagc aagatcatct ggtagaagag gttgccagag
     4741 cagttttatc tgattcacca cagctctctg aaggaaaaga aataaaatta gaggaactaa
     4801 ttgactctct gggttctaac cccttcttaa caaggaatca gattccccgt actccagaaa
     4861 acttgataac tgaaattagg agctcatgga gaaaagctat tgaaatggaa gaaaacagaa
     4921 ctaaagaacc aattcaaatg gatgttgaac atagagaagt attgccagaa tcattacctg
     4981 tgttgcacaa tcaaagagaa tttagcatgg ctgattttct cttagaaacc actgtatcag
     5041 attttggcca gtctcatttg actgaagaga aagttatttc agattgcgag tgtgtgcctc
     5101 agaaacatgt gctgaccagt cacatagatg aaccaccaac acaaaatcag tcagatttgt
     5161 taaataagaa agtaatttgc aagcaagatt tggaatgttt agccttcacc aagctttcag
     5221 aaactagccg aatggagaca ttctcccctg ctgtcggcaa taggatagat gtgatgggtg
     5281 gcagtgaaga ggagtttatg aaaatattgg accacttaga agtttcttgt aacaaacctt
     5341 ccacaaataa aactatgttg tggaattctt ttcagatatc aattggaatt agttctaaga
     5401 gttttaaaga taatgatttt ggcatattac acgaaactct cccggaagaa gttggtcatc
     5461 taagttttaa tagttccagt agttcagagg ccaattttaa actggagcca aatagtccta
     5521 tgcatggtgg cactcttcta gaagatgttg tgggagggag acagactact ccagaatcag
     5581 actttaattt acaggctctt cgcagtagat acgaggctct gaagaaatct ctttccaaga
     5641 aaagggaaga atcttacctc tcgaattccc aaacacccga aagacacaaa ccagaattga
     5701 gccctactcc ccaaaatgta caaacagatg atacgcttaa ctttttggac acctgtgatt
     5761 tgcatactga gcatataaag ccatctttac gcacgtccat cggtgaaaga aaacggtctc
     5821 tttcaccact aattaagttt tctccagtgg aacaaagatt gagaaccaca atagcatgta
     5881 gtcttggaga actacctaat ttaaaggaag aagacatttt gaataagagc cttgatgcaa
     5941 aagaaccacc gtctgacttg acaagatgag gatccgaatt ccgatccagt gtgctggaat
     6001 taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     6061 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     6121 cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt
     6181 gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc
     6241 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca
     6301 tctagggcgg ccaattccgc ccctctcccc ccccccccta acgttactgg ccgaagccgc
     6361 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     6421 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     6481 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     6541 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     6601 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     6661 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     6721 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     6781 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     6841 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac
     6901 aacccacaag gagacgacct tccatggcca agcctttgtc tcaagaagaa tccaccctca
     6961 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     7021 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     7081 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     7141 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     7201 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     7261 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     7321 aacgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga aagattgact
     7381 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7441 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7501 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7561 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7621 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7681 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7741 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7801 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7861 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7921 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     7981 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     8041 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     8101 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     8161 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     8221 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     8281 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     8341 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8401 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     8461 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8521 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8581 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     8641 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8701 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8761 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8821 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8881 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     8941 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     9001 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     9061 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     9121 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9181 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9241 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9301 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9361 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9421 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9481 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9541 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9601 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     9661 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     9721 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     9781 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     9841 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     9901 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     9961 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
    10021 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
    10081 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
    10141 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
    10201 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    10261 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    10321 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10441 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    10501 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10561 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10621 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10681 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    11041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    11101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    11161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    11221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    11341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11461 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11521 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11581 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11641 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11701 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11761 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11821 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11881 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11941 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    12001 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    12061 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    12121 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    12181 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    12241 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    12301 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    12361 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    12421 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12481 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12541 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12601 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
