LOCUS       sequence-367932-       13219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector for production of lentivirus. Expresses 
            EGFP-P2A-HAUS6 (siRNA resistant version).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   David AF, Roudot P, Legant WR, Betzig E, Danuser G, Gerlich DW
  TITLE     Augmin accumulation on long-lived microtubules drives amplification 
            and kinetochore-directed growth.
  JOURNAL   J Cell Biol. 2019 Jul 1;218(7):2150-2168. doi: 
            10.1083/jcb.201805044. Epub 2019 May 21.
  PUBMED    31113824
REFERENCE   2  (bases 1 to 13219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201805044"; journalName: "J Cell Biol"; date: 
            "2019-07-1- 1"; volume: "218"; issue: "7"; pages: "2150-2168"
COMMENT     Sequence Label: Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast
FEATURES             Location/Qualifiers
     source          1..13219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2649..2860
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2875..2884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2881..2883,2884..2886,2887..3597)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2881..2883,2884..2886,2887..3597)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2926..2947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3187..3206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3534..3555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3607..3663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     intron          6601..6830
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6813..6832
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6888..7459
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7053..7070)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7280..7299
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7492..7890
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7910..8498
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7963..7983)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8381..8392)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8501..8517)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8502..8518)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9023..9203
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9229..9246)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9235..9459
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9505..9933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9592..9611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9802..9823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9942..9962)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9947..10276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10127..10262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10189..10208
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10324..10371
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10390..10764
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10894..11015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10931..10950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10985..11004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11064..11080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11064..11080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11077..11099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11088..11104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11112..11118,11119..11136,11137..11142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11157..11178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11295..11312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11466..12054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11546..11565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12225..13016,13017..13085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12848..12867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13086..13190)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattag ctagctaggt cttgaaagga gtgggaattg gctccggtgc
     2641 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2701 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2761 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2821 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gagaattctc tagagccacc
     2881 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2941 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctat
     3001 ggtaagctta ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3061 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3121 cagcacgact ttttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3181 tttaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3241 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3301 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     3361 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3421 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3481 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3541 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
     3601 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     3661 ccgaccggta tgagctcggc ctcggtcacc gctttcgaga aggagcatct ctggatgtat
     3721 ctgcaggcgc tcggcttcga gccaggcccg gcaaccattg cctgcggaaa gatcgtgtcg
     3781 cacacgcacc tcggagtgaa catgtttgac aagctgaacc gtgatgcctt tcatataatt
     3841 tcttattttt tgtttcaagt tctggaccag tctctcacca aagaagtttt caaattttgt
     3901 tggcccccat ttgaccaaaa aagtgacact gaattccgaa aacattgctg tgaatggata
     3961 aaaaggattt ctggtgaatg tggaagcagc tttcctcaag ttgttggttc actatttctt
     4021 tctcctggtg gtcctaagtt tattcatctg atgtatcatt ttgcaagatt tgttgcaatg
     4081 aaatatatta aatccaattc taaaaattct tctcatcatt ttgtagagac atttaacata
     4141 aaaccacagg acttgcacaa atgcattgcc agatgccatt tcgcacgtag cagattttta
     4201 caaattttgc aaagacaaga ttgtgttacc caaaaatatc aggaaaatgc acaattatct
     4261 gtcaagcaag ttcgtaactt gagatctgaa tgtataggat tggaaaacca aataaagaaa
     4321 atggaaccct atgatgacca cagtaatatg gaagaaaaaa ttcaaaaggt tcggtctttg
     4381 tgggcttcag tgaatgaaac gctcatgttt ttggaaaaag agagagaagt tgttagttcg
     4441 gtccttagtc ttgttaacca atatgcttta gatggaacta atgttgctat taatattcca
     4501 aggctcttac ttgacaaaat tgagaaacaa atgtttcagt tgcacatagg aaatgtttat
     4561 gaggctggaa aactgaacct cttaacagtt attcagttat taaatgaagt cttgaaggtg
     4621 atgaaatatg aacgttgtca ggctgatcaa gcaagattga cggtagacct tcactacctt
     4681 gaaaaagaga ccaaatttca gaaggaaaga ttatcagatc tgaaacatat gaggtataga
     4741 ataaaagatg atctcacaac tataagacat tctgttgttg aaaagcaagg agaatggcat
     4801 aaaaagtgga aagaatttct tggtttgtct cctttcagtc taattaaagg ttggactcca
     4861 tctgtagatc ttttaccacc aatgtctccc ctttcgtttg atcctgcctc agaagaagtg
     4921 tatgcaaaga gtattctttg tcagtatcct gcttcacttc cagatgcaca taagcaacat
     4981 aaccaagaaa atggttgcag aggagacagt gataccttgg gagcgctaca tgatctagcc
     5041 aacagccctg cctctttctt gtcgcagtca gtttcatcat cagatagaaa cagtgttaca
     5101 gtacttgaaa aggacacaaa gatgggaact cccaaagaaa aaaatgaagc aatttctaag
     5161 aaaataccag aatttgaagt ggaaaattct ccattatcag atgttgcaaa gaatacagag
     5221 agtagtgcat ttggagggtc tttgccagct aaaaaaagtg atccatttca aaaagagcaa
     5281 gatcatctgg tagaagaggt tgccagagca gttttatctg attcaccaca gctctctgaa
     5341 ggaaaagaaa taaaattaga ggaactaatt gactctctgg gttctaaccc cttcttaaca
     5401 aggaatcaga ttccccgtac tccagaaaac ttgataactg aaattaggag ctcatggaga
     5461 aaagctattg aaatggaaga aaacagaact aaagaaccaa ttcaaatgga tgttgaacat
     5521 agagaagtat tgccagaatc attacctgtg ttgcacaatc aaagagaatt tagcatggct
     5581 gattttctct tagaaaccac tgtatcagat tttggccagt ctcatttgac tgaagagaaa
     5641 gttatttcag attgcgagtg tgtgcctcag aaacatgtgc tgaccagtca catagatgaa
     5701 ccaccaacac aaaatcagtc agatttgtta aataagaaag taatttgcaa gcaagatttg
     5761 gaatgtttag ccttcaccaa gctttcagaa actagccgaa tggagacatt ctcccctgct
     5821 gtcggcaata ggatagatgt gatgggtggc agtgaagagg agtttatgaa aatattggac
     5881 cacttagaag tttcttgtaa caaaccttcc acaaataaaa ctatgttgtg gaattctttt
     5941 cagatatcaa ttggaattag ttctaagagt tttaaagata atgattttgg catattacac
     6001 gaaactctcc cggaagaagt tggtcatcta agttttaata gttccagtag ttcagaggcc
     6061 aattttaaac tggagccaaa tagtcctatg catggtggca ctcttctaga agatgttgtg
     6121 ggagggagac agactactcc agaatcagac tttaatttac aggctcttcg cagtagatac
     6181 gaggctctga agaaatctct ttccaagaaa agggaagaat cttacctctc gaattcccaa
     6241 acacccgaaa gacacaaacc agaattgagc cctactcccc aaaatgtaca aacagatgat
     6301 acgcttaact ttttggacac ctgtgatttg catactgagc atataaagcc atctttacgc
     6361 acgtccatcg gtgaaagaaa acggtctctt tcaccactaa ttaagttttc tccagtggaa
     6421 caaagattga gaaccacaat agcatgtagt cttggagaac tacctaattt aaaggaagaa
     6481 gacattttga ataagagcct tgatgcaaaa gaaccaccgt ctgacttgac aagatgagga
     6541 tccgaattcc gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
     6601 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
     6661 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
     6721 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
     6781 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
     6841 ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc
     6901 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     6961 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     7021 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     7081 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     7141 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     7201 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     7261 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     7321 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     7381 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     7441 ttgaaaaaca cgatgataag cttgccacaa cccacaagga gacgaccttc catggccaag
     7501 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     7561 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     7621 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     7681 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     7741 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     7801 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     7861 ctgccctctg gttatgtgtg ggagggctaa cgatatcaag cttatcggta atcaacctct
     7921 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7981 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     8041 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     8101 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     8161 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     8221 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     8281 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     8341 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8401 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8461 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
     8521 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     8581 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     8641 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     8701 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     8761 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     8821 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     8881 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     8941 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     9001 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9061 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9181 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     9241 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     9301 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9361 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9421 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     9481 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9541 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9601 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9661 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9721 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9781 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9841 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9901 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     9961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    10021 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10081 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    10141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10201 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10261 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
    10321 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
    10381 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
    10441 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
    10501 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
    10561 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
    10621 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
    10681 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
    10741 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
    10801 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    10861 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    10921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    11041 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    11101 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    11161 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    11221 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    11281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    11341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    11401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    11461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    11521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    11581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    11641 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    11701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    11761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    11821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11881 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    11941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    12001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    12061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    12121 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    12181 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    12241 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    12301 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    12361 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    12421 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    12481 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    12541 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    12601 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    12661 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    12721 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    12781 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    12841 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    12901 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12961 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    13021 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    13081 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    13141 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    13201 cgaaaagtgc cacctgacg
//
