LOCUS       sequence-367936-       16330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-mCherry-Ki67.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16330)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 16330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: EGFP-mCherry-Ki67
FEATURES             Location/Qualifiers
     source          1..16330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     461..481
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(588..590,591..1298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITLGMDELY"
     primer_bind     complement(630..651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(891..910)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1311..2015
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1453..1471)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1721..1740
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5611..5622
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5977..5988
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6703..6714
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7435..7446
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8887..8898
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     intron          11881..12110
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     12093..12112
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    12168..12741
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12335..12352)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12562..12581
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12774..13373
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12774..12793)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13270..13290
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(13509..13526)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13515..13739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13786..13805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13865..13881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13865..13881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13878..13900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13889..13905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13913..13919,13920..13937,13938..13943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13958..13979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14096..14113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14267..14855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14347..14366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15026..15817,15818..15886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15649..15668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15887..15991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16066..16085)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(16257..16330,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaccgccat ggctagcgtg agcaagggcg
      601 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      661 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      721 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      781 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      841 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      901 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      961 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1021 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1081 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1141 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1201 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtccttaag gagttcgtga
     1261 ccgccgccgg gatcactctc ggcatggacg agctgtacgg caagggaagc gtgagcaagg
     1321 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     1381 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1441 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     1501 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     1561 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1621 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     1681 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     1741 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     1801 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     1861 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     1921 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     1981 gccactccac cggcggcatg gacgagctgt acaagagcag cggccccagc ggcagcagca
     2041 gctggcccac gagacgcctg gttactatca aaaggagcgg ggtcgacggt ccccactttc
     2101 ccctgagcct cagcacctgc ttgtttggaa ggggtattga atgtgacatc cgtatccagc
     2161 ttcctgttgt gtcaaaacaa cattgcaaaa ttgaaatcca tgagcaggag gcaatattac
     2221 ataatttcag ttccacaaat ccaacacaag taaatgggtc tgttattgat gagcctgtac
     2281 ggctaaaaca tggagatgta ataactatta ttgatcgttc cttcaggtat gaaaatgaaa
     2341 gtcttcagaa tggaaggaag tcaactgaat ttccaagaaa aatacgtgaa caggagccag
     2401 cacgtcgtgt ctcaagatct agcttctctt ctgaccctga tgagaaagct caagattcca
     2461 aggcctattc aaaaatcact gaaggaaaag tttcaggaaa tcctcaggta catatcaaga
     2521 atgtcaaaga agacagtacc gcagatgact caaaagacag tgttgctcag ggaacaacta
     2581 atgttcattc ctcagaacat gctggacgta atggcagaaa tgcagctgat cccatttctg
     2641 gggattttaa agaaatttcc agcgttaaat tagtgagccg ttatggagaa ttgaagtctg
     2701 ttcccactac acaatgtctt gacaatagca aaaaaaatga atctcccttt tggaagcttt
     2761 atgagtcagt gaagaaagag ttggatgtaa aatcacaaaa agaaaatgtc ctacagtatt
     2821 gtagaaaatc tggattacaa actgattacg caacagagaa agaaagtgct gatggtttac
     2881 agggggagac ccaactgttg gtctcgcgta agtcaagacc aaaatctggt gggagcggcc
     2941 acgctgtggc agagcctgct tcacctgaac aagagcttga ccagaacaag gggaagggaa
     3001 gagacgtgga gtctgttcag actcccagca aggctgtggg cgccagcttt cctctctatg
     3061 agccggctaa aatgaagacc cccgtacaat attcacagca acaaaattct ccacaaaaac
     3121 ataagaacaa agacctgtat actactggta gaagagaatc tgtgaatctg ggtaaaagtg
     3181 aaggcttcaa ggctggtgat aaaactctta ctcccaggaa gctttcaact agaaatcgaa
     3241 caccagctaa agttgaagat gcagctgact ctgccactaa gccagaaaat ctctcttcca
     3301 aaaccagagg aagtattcct acagatgtgg aagttctgcc tacggaaact gaaattcaca
     3361 atgagccatt tttaactctg tggctcactc aagttgagag gaagatccaa aaggattccc
     3421 tcagcaagcc tgagaaattg ggcactacag ctggacagat gtgctctggg ttacctggtc
     3481 ttagttcagt tgatatcaac aactttggtg attccattaa tgagagtgag ggaatacctt
     3541 tgaaaagaag gcgtgtgtcc tttggtgggc acctaagacc tgaactattt gatgaaaact
     3601 tgcctcctaa tacgcctctc aaaaggggag aagccccaac caaaagaaag tctctggtaa
     3661 tgcacactcc acctgtcctg aagaaaatca tcaaggaaca gcctcaacca tcaggaaaac
     3721 aagagtcagg ttcagaaatc catgtggaag tgaaggcaca aagcttggtt ataagccctc
     3781 cagctcctag tcctaggaaa actccagttg ccagtgatca acgccgtagg tcctgcaaaa
     3841 cagcccctgc ttccagcagc aaatctcaga cagaggttcc taagagagga gggagaaaga
     3901 gtggcaacct gccttcaaag agagtgtcta tcagccgaag tcaacatgat attttacaga
     3961 tgatatgttc caaaagaaga agtggtgctt cggaagcaaa tctgattgtt gcaaaatcat
     4021 gggcagatgt agtaaaactt ggtgcaaaac aaacacaaac taaagtcata aaacatggtc
     4081 ctcaaaggtc aatgaacaaa aggcaaagaa gacctgctac tccaaagaag cctgtgggcg
     4141 aagttcacag tcaatttagt acaggccacg caaactctcc ttgtaccata ataataggga
     4201 aagctcatac tgaaaaagta catgtgcctg ctcgacccta cagagtgctc aacaacttca
     4261 tttccaacca aaaaatggac tttaaggaag atctttcagg aatagctgaa atgttcaaga
     4321 ccccagtgaa ggagcaaccg cagttgacaa gcacatgtca catcgctatt tcaaattcag
     4381 agaatttgct tggaaaacag tttcaaggaa ctgattcagg agaagaacct ctgctcccca
     4441 cctcagagag ttttggagga aatgtgttct tcagtgcaca gaatgcagca aaacagccat
     4501 ctgataaatg ctctgcaagc cctcccttaa gacggcagtg tattagagaa aatggaaacg
     4561 tagcaaaaac gcccaggaac acctacaaaa tgacttctct ggagacaaaa acttcagata
     4621 ctgagacaga gccttcaaaa acagtatcca ctgcaaacag gtcaggaagg tctacagagt
     4681 tcaggaatat acagaagcta cctgtggaaa gtaagagtga agaaacaaat acagaaattg
     4741 ttgagtgcat cctaaaaaga ggtcagaagg caacactact acaacaaagg agagaaggag
     4801 agatgaagga aatagaaaga ccttttgaga catataagga aaatattgaa ttaaaagaaa
     4861 acgatgaaaa gatgaaagca atgaagagat caagaacttg ggggcagaaa tgtgcaccaa
     4921 tgtctgacct gacagacctc aagagcttgc ctgatacaga actcatgaaa gacacggcac
     4981 gtggccagaa tctcctccaa acccaagatc atgccaaggc accaaagagt gagaaaggca
     5041 aaatcactaa aatgccctgc cagtcattac aaccagaacc aataaacacc ccaacacaca
     5101 caaaacaaca gttgaaggca tccctgggga aagtaggtgt gaaagaagag ctcctagcag
     5161 tcggcaagtt cacacggacg tcaggggaga ccacgcacac gcacagagag ccagcaggag
     5221 atggcaagag catcagaacg tttaaggagt ctccaaagca gatcctggac ccagcagccc
     5281 gtgtaactgg aatgaagaag tggccaagaa cgcctaagga agaggcccag tcactagaag
     5341 acctggctgg cttcaaagag ctcttccaga caccaggtcc ctctgaggaa tcaatgactg
     5401 atgagaaaac taccaaaata gcctgcaaat ctccaccacc agaatcagtg gacactccaa
     5461 caagcacaaa gcaatggcct aagagaagtc tcaggaaagc agatgtagag gaagaattct
     5521 tagcactcag gaaactaaca ccatcagcag ggaaagccat gcttacgccc aaaccagcag
     5581 gaggtgatga gaaagacatt aaagcattta tgggaactcc agtgcagaaa ctggacctgg
     5641 caggaacttt acctggcagc aaaagacagc tacagactcc taaggaaaag gcccaggctc
     5701 tagaagacct ggctggcttt aaagagctct tccagactcc tggtcacacc gaggaattag
     5761 tggctgctgg taaaaccact aaaataccct gcgactctcc acagtcagac ccagtggaca
     5821 ccccaacaag cacaaagcaa cgacccaaga gaagtatcag gaaagcagat gtagagggag
     5881 aactcttagc gtgcaggaat ctaatgccat cagcaggcaa agccatgcac acgcctaaac
     5941 catcagtagg tgaagagaaa gacatcatca tatttgtggg aactccagtg cagaaactgg
     6001 acctgacaga gaacttaacc ggcagcaaga gacggccaca aactcctaag gaagaggccc
     6061 aggctctgga agacctgact ggctttaaag agctcttcca gacccctggt catactgaag
     6121 aagcagtggc tgctggcaaa actactaaaa tgccctgcga atcttctcca ccagaatcag
     6181 cagacacccc aacaagcaca agaaggcagc ccaagacacc tttggagaaa agggacgtac
     6241 agaaggagct ctcagccctg aagaagctca cacagacatc aggggaaacc acacacacag
     6301 ataaagtacc aggaggtgag gataaaagca tcaacgcgtt tagggaaact gcaaaacaga
     6361 aactggaccc agcagcaagt gtaactggta gcaagaggca cccaaaaact aaggaaaagg
     6421 cccaacccct agaagacctg gctggcttga aagagctctt ccagacacca gtatgcactg
     6481 acaagcccac gactcacgag aaaactacca aaatagcctg cagatcacaa ccagacccag
     6541 tggacacacc aacaagctcc aagccacagt ccaagagaag tctcaggaaa gtggacgtag
     6601 aagaagaatt cttcgcactc aggaaacgaa caccatcagc aggcaaagcc atgcacacac
     6661 ccaaaccagc agtaagtggt gagaaaaaca tctacgcatt tatgggaact ccagtgcaga
     6721 aactggacct gacagagaac ttaactggca gcaagagacg gctacaaact cctaaggaaa
     6781 aggcccaggc tctagaagac ctggctggct ttaaagagct cttccagaca cgaggtcaca
     6841 ctgaggaatc aatgactaac gataaaactg ccaaagtagc ctgcaaatct tcacaaccag
     6901 acccagacaa aaacccagca agctccaagc gacggctcaa gacatccctg gggaaagtgg
     6961 gcgtgaaaga agagctccta gcagttggca agctcacaca gacatcagga gagactacac
     7021 acacacacac agagccaaca ggagatggta agagcatgaa agcatttatg gagtctccaa
     7081 agcagatctt agactcagca gcaagtctaa ctggcagcaa gaggcagctg agaactccta
     7141 agggaaagtc tgaagtccct gaagacctgg ccggcttcat cgagctcttc cagacaccaa
     7201 gtcacactaa ggaatcaatg actaatgaaa aaactaccaa agtatcctac agagcttcac
     7261 agccagacct agtggacacc ccaacaagct ccaagccaca gcccaagaga agtctcagga
     7321 aagcagacac tgaagaagaa tttttagcat ttaggaaaca aacgccatca gcaggcaaag
     7381 ccatgcacac acccaaacca gcagtaggtg aagagaaaga catcaacacg tttttgggaa
     7441 ctccagtgca gaaactggac cagccaggaa atttacctgg cagcaataga cggctacaaa
     7501 ctcgtaagga aaaggcccag gctctagaag aactgactgg cttcagagag cttttccaga
     7561 caccatgcac tgataacccc acgactgatg agaaaactac caaaaaaata ctctgcaaat
     7621 ctccgcaatc agacccagcg gacaccccaa caaacacaaa gcaacggccc aagagaagcc
     7681 tcaagaaagc agacgtagag gaagaatttt tagcattcag gaaactaaca ccatcagcag
     7741 gcaaagccat gcacacgcct aaagcagcag taggtgaaga gaaagacatc aacacatttg
     7801 tggggactcc agtggagaaa ctggacctgc taggaaattt acctggcagc aagagacggc
     7861 cacaaactcc taaagaaaag gccaaggctc tagaagatct ggctggcttc aaagagctct
     7921 tccagacacc aggtcacact gaggaatcaa tgaccgatga caaaatcaca gaagtatcct
     7981 gcaaatctcc acaaccagac ccagtcaaaa ccccaacaag ctccaagcaa cgactcaaga
     8041 tatccttggg gaaagtaggt gtgaaagaag aggtcctacc agtcggcaag ctcacacaga
     8101 cgtcagggaa gaccacacag acacacagag agacagcagg agatggaaag agcatcaaag
     8161 cgtttaagga atctgcaaag cagatgctgg acccagcaaa ctatggaact gggatggaga
     8221 ggtggccaag aacacctaag gaagaggccc aatcactaga agacctggcc ggcttcaaag
     8281 agctcttcca gacaccagac cacactgagg aatcaacaac tgatgacaaa actaccaaaa
     8341 tagcctgcaa atctccacca ccagaatcaa tggacactcc aacaagcaca aggaggcggc
     8401 ccaaaacacc tttggggaaa agggatatag tggaagagct ctcagccctg aagcagctca
     8461 cacagaccac acacacagac aaagtaccag gagatgagga taaaggcatc aacgtgttca
     8521 gggaaactgc aaaacagaaa ctggacccag cagcaagtgt aactggtagc aagaggcagc
     8581 caagaactcc taagggaaaa gcccaacccc tagaagactt ggctggcttg aaagagctct
     8641 tccagacacc aatatgcact gacaagccca cgactcatga gaaaactacc aaaatagcct
     8701 gcagatctcc acaaccagac ccagtgggta ccccaacaat cttcaagcca cagtccaaga
     8761 gaagtctcag gaaagcagac gtagaggaag aatccttagc actcaggaaa cgaacaccat
     8821 cagtagggaa agctatggac acacccaaac cagcaggagg tgatgagaaa gacatgaaag
     8881 catttatggg aactccagtg cagaaattgg acctgccagg aaatttacct ggcagcaaaa
     8941 gatggccaca aactcctaag gaaaaggccc aggctctaga agacctggct ggcttcaaag
     9001 agctcttcca gacaccaggc actgacaagc ccacgactga tgagaaaact accaaaatag
     9061 cctgcaaatc tccacaacca gacccagtgg acaccccagc aagcacaaag caacggccca
     9121 agagaaacct caggaaagca gacgtagagg aagaattttt agcactcagg aaacgaacac
     9181 catcagcagg caaagccatg gacacaccaa aaccagcagt aagtgatgag aaaaatatca
     9241 acacatttgt ggaaactcca gtgcagaaac tggacctgct aggaaattta cctggcagca
     9301 agagacagcc acagactcct aaggaaaagg ctgaggctct agaggacctg gttggcttca
     9361 aagaactctt ccagacacca ggtcacactg aggaatcaat gactgatgac aaaatcacag
     9421 aagtatcctg taaatctcca cagccagagt cattcaaaac ctcaagaagc tccaagcaaa
     9481 ggctcaagat acccctggtg aaagtggaca tgaaagaaga gcccctagca gtcagcaagc
     9541 tcacacggac atcaggggag actacgcaaa cacacacaga gccaacagga gatagtaaga
     9601 gcatcaaagc gtttaaggag tctccaaagc agatcctgga cccagcagca agtgtaactg
     9661 gtagcaggag gcagctgaga actcgtaagg aaaaggcccg tgctctagaa gacctggttg
     9721 acttcaaaga gctcttctca gcaccaggtc acactgaaga gtcaatgact attgacaaaa
     9781 acacaaaaat tccctgcaaa tctcccccac cagaactaac agacactgcc acgagcacaa
     9841 agagatgccc caagacacgt cccaggaaag aagtaaaaga ggagctctca gcagttgaga
     9901 ggctcacgca aacatcaggg caaagcacac acacacacaa agaaccagca agcggtgatg
     9961 agggcatcaa agtattgaag caacgtgcaa agaagaaacc aaacccagta gaagaggaac
    10021 ccagcaggag aaggccaaga gcacctaagg aaaaggccca acccctggaa gacctggccg
    10081 gcttcacaga gctctctgaa acatcaggtc acactcagga atcactgact gctggcaaag
    10141 ccactaaaat accctgcgaa tctcccccac tagaagtggt agacaccaca gcaagcacaa
    10201 agaggcatct caggacacgt gtgcagaagg tacaagtaaa agaagagcct tcagcagtca
    10261 agttcacaca aacatcaggg gaaaccacgg atgcagacaa agaaccagca ggtgaagata
    10321 aaggcatcaa agcattgaag gaatctgcaa aacagacacc ggctccagca gcaagtgtaa
    10381 ctggcagcag gagacggcca agagcaccca gggaaagtgc ccaagccata gaagacctag
    10441 ctggcttcaa agacccagca gcaggtcaca ctgaagaatc aatgactgat gacaaaacca
    10501 ctaaaatacc ctgcaaatca tcaccagaac tagaagacac cgcaacaagc tcaaagagac
    10561 ggcccaggac acgtgcccag aaagtagaag tgaaggagga gctgttagca gttggcaagc
    10621 tcacacaaac ctcaggggag accacgcaca ccgacaaaga gccggtaggt gagggcaaag
    10681 gcacgaaagc atttaagcaa cctgcaaagc ggaagctgga cgcagaagat gtaattggca
    10741 gcaggagaca gccaagagca cctaaggaaa aggcccaacc cctggaagat ctggccagct
    10801 tccaagagct ctctcaaaca ccaggccaca ctgaggaact ggcaaatggt gctgctgata
    10861 gctttacaag cgctccaaag caaacacctg acagtggaaa acctctaaaa atatccagaa
    10921 gagttcttcg ggcccctaaa gtagaacccg tgggagacgt ggtaagcacc agagaccctg
    10981 taaaatcaca aagcaaaagc aacacttccc tgcccccact gcccttcaag aggggaggtg
    11041 gcaaagatgg aagcgtcacg ggaaccaaga ggttgcgctg catgccagca ccagaggaaa
    11101 ttgtggagga gctgccagcc agcaagaagc agagggttgc tcccagggca agaggcaaat
    11161 catccgaacc cgtggtcatc atgaagagaa gtttgaggac ttctgcaaaa agaattgaac
    11221 ctgcggaaga gctgaacagc aacgacatga aaaccaacaa agaggaacac aaattacaag
    11281 actcggtccc tgaaaataag ggaatatccc tgcgctccag acgccaaaat aagactgagg
    11341 cagaacagca aataactgag gtctttgtat tagcagaaag aatagaaata aacagaaatg
    11401 aaaagaagcc catgaagacc tccccagaga tggacattca gaatccagat gatggagccc
    11461 ggaaacccat acctagagac aaagtcactg agaacaaaag gtgcttgagg tctgctagac
    11521 agaatgagag ctcccagcct aaggtggcag aggagagcgg agggcagaag agtgcgaagg
    11581 ttctcatgca gaatcagaaa gggaaaggag aagcaggaaa ttcagactcc atgtgcctga
    11641 gatcaagaaa gacaaaaagc cagcctgcag caagcacttt ggagagcaaa tctgtgcaga
    11701 gagtaacgcg gagtgtcaag aggtgtgcag aaaatccaaa gaaggctgag gacaatgtgt
    11761 gtgtcaagaa aataagaacc agaagtcata gggacagtga agatatttgg gatcggccgc
    11821 atagataact gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
    11881 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
    11941 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
    12001 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
    12061 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
    12121 ccaggtccaa ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc
    12181 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
    12241 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
    12301 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
    12361 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
    12421 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
    12481 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
    12541 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
    12601 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
    12661 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
    12721 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg
    12781 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc
    12841 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca
    12901 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
    12961 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
    13021 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
    13081 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
    13141 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
    13201 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
    13261 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
    13321 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
    13381 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc
    13441 acccggggcg gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagagctcg
    13501 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
    13561 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
    13621 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
    13681 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
    13741 ttctgaggcg gaaagaacca gctggggctc gagtgcattc tagttgtggt ttgtccaaac
    13801 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    13861 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    13921 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    13981 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    14041 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    14101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    14161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    14221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    14281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    14341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    14401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    14461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    14521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    14581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    14641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    14701 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    14761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    14821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    14881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    14941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    15001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    15061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    15121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    15181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    15241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    15301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    15361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    15421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    15481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    15541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    15601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    15661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    15721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    15781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    15841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    15901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    15961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    16021 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
    16081 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
    16141 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
    16201 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
    16261 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    16321 tatggagttc
//
