LOCUS       sequence-367957-       15194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ki67∆FHA-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15194)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 15194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: Ki67∆FHA-mNeonGreen-IRESpuro2
FEATURES             Location/Qualifiers
     source          1..15194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     461..481
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3746..3757
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4112..4123
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4838..4849
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5570..5581
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7022..7033
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             9952..10662
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      10207..10216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          10731..10960
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     10943..10962
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(11032..11606,11607..11609,11610..11618)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11199..11216)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11426..11445
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11638..12237
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(11638..11657)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12134..12154
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(12373..12390)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12379..12603
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     12650..12669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12729..12745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12729..12745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12742..12764)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12753..12769
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12777..12783,12784..12801,12802..12807))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12822..12843
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12960..12977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13131..13719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13211..13230)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13890..14681,14682..14750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14513..14532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14751..14855)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14930..14949)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(15121..15194,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaatggaga aagctcaaga ttccaaggcc
      601 tattcaaaaa tcactgaagg aaaagtttca ggaaatcctc aggtacatat caagaatgtc
      661 aaagaagaca gtaccgcaga tgactcaaaa gacagtgttg ctcagggaac aactaatgtt
      721 cattcctcag aacatgctgg acgtaatggc agaaatgcag ctgatcccat ttctggggat
      781 tttaaagaaa tttccagcgt taaattagtg agccgttatg gagaattgaa gtctgttccc
      841 actacacaat gtcttgacaa tagcaaaaaa aatgaatctc ccttttggaa gctttatgag
      901 tcagtgaaga aagagttgga tgtaaaatca caaaaagaaa atgtcctaca gtattgtaga
      961 aaatctggat tacaaactga ttacgcaaca gagaaagaaa gtgctgatgg tttacagggg
     1021 gagacccaac tgttggtctc gcgtaagtca agaccaaaat ctggtgggag cggccacgct
     1081 gtggcagagc ctgcttcacc tgaacaagag cttgaccaga acaaggggaa gggaagagac
     1141 gtggagtctg ttcagactcc cagcaaggct gtgggcgcca gctttcctct ctatgagccg
     1201 gctaaaatga agacccctgt acaatattca cagcaacaaa attctccaca aaaacataag
     1261 aacaaagacc tgtatactac tggtagaaga gaatctgtga atctgggtaa aagtgaaggc
     1321 ttcaaggctg gtgataaaac tcttactccc aggaagcttt caactagaaa tcgaacacca
     1381 gctaaagttg aagatgcagc tgactctgcc actaagccag aaaatctctc ttccaaaacc
     1441 agaggaagta ttcctacaga tgtggaagtt ctgcctacgg aaactgaaat tcacaatgag
     1501 ccatttttaa ctctgtggct cactcaagtt gagaggaaga tccaaaagga ttccctcagc
     1561 aagcctgaga aattgggcac tacagctgga cagatgtgct ctgggttacc tggtcttagt
     1621 tcagttgata tcaacaactt tggtgattcc attaatgaga gtgagggaat acctttgaaa
     1681 agaaggcgtg tgtcctttgg tgggcaccta agacctgaac tatttgatga aaacttgcct
     1741 cctaatacgc ctctcaaaag gggagaagcc ccaaccaaaa gaaagtctct ggtaatgcac
     1801 actccacctg tcctgaagaa aatcatcaag gaacagcctc aaccatcagg aaaacaagag
     1861 tcaggttcag aaatccatgt ggaagtgaag gcacaaagct tggttataag ccctccagct
     1921 cctagtccta ggaaaactcc agttgccagt gatcaacgcc gtaggtcctg caaaacagcc
     1981 cctgcttcca gcagcaaatc tcagacagag gttcctaaga gaggagggag aaagagtggc
     2041 aacctgcctt caaagagagt gtctatcagc cgaagtcaac atgatatttt acagatgata
     2101 tgttccaaaa gaagaagtgg tgcttcggaa gcaaatctga ttgttgcaaa atcatgggca
     2161 gatgtagtaa aacttggtgc aaaacaaaca caaactaaag tcataaaaca tggtcctcaa
     2221 aggtcaatga acaaaaggca aagaagacct gctactccaa agaagcctgt gggcgaagtt
     2281 cacagtcaat ttagtacagg ccacgcaaac tctccttgta ccataataat agggaaagct
     2341 catactgaaa aagtacatgt gcctgctcga ccctacagag tgctcaacaa cttcatttcc
     2401 aaccaaaaaa tggactttaa ggaagatctt tcaggaatag ctgaaatgtt caagacccca
     2461 gtgaaggagc aaccgcagtt gacaagcaca tgtcacatcg ctatttcaaa ttcagagaat
     2521 ttgcttggaa aacagtttca aggaactgat tcaggagaag aacctctgct ccccacctca
     2581 gagagttttg gaggaaatgt gttcttcagt gcacagaatg cagcaaaaca gccatctgat
     2641 aaatgctctg caagccctcc cttaagacgg cagtgtatta gagaaaatgg aaacgtagca
     2701 aaaacgccca ggaacaccta caaaatgact tctctggaga caaaaacttc agatactgag
     2761 acagagcctt caaaaacagt atccactgca aacaggtcag gaaggtctac agagttcagg
     2821 aatatacaga agctacctgt ggaaagtaag agtgaagaaa caaatacaga aattgttgag
     2881 tgcatcctaa aaagaggtca gaaggcaaca ctactacaac aaaggagaga aggagagatg
     2941 aaggaaatag aaagaccttt tgagacatat aaggaaaata ttgaattaaa agaaaacgat
     3001 gaaaagatga aagcaatgaa gagatcaaga acttgggggc agaaatgtgc accaatgtct
     3061 gacctgacag acctcaagag cttgcctgat acagaactca tgaaagacac ggcacgtggc
     3121 cagaatctcc tccaaaccca agatcatgcc aaggcaccaa agagtgagaa aggcaaaatc
     3181 actaaaatgc cctgccagtc attacaacca gaaccaataa acaccccaac acacacaaaa
     3241 caacagttga aggcatccct ggggaaagta ggtgtgaaag aagagctcct agcagtcggc
     3301 aagttcacac ggacgtcagg ggagaccacg cacacgcaca gagagccagc aggagatggc
     3361 aagagcatca gaacgtttaa ggagtctcca aagcagatcc tggacccagc agcccgtgta
     3421 actggaatga agaagtggcc aagaacgcct aaggaagagg cccagtcact agaagacctg
     3481 gctggcttca aagagctctt ccagacacca ggtccctctg aggaatcaat gactgatgag
     3541 aaaactacca aaatagcctg caaatctcca ccaccagaat cagtggacac tccaacaagc
     3601 acaaagcaat ggcctaagag aagtctcagg aaagcagatg tagaggaaga attcttagca
     3661 ctcaggaaac taacaccatc agcagggaaa gccatgctta cgcccaaacc agcaggaggt
     3721 gatgagaaag acattaaagc atttatggga actccagtgc agaaactgga cctggcagga
     3781 actttacctg gcagcaaaag acagctacag actcctaagg aaaaggccca ggctctagaa
     3841 gacctggctg gctttaaaga gctcttccag actcctggtc acaccgagga attagtggct
     3901 gctggtaaaa ccactaaaat accctgcgac tctccacagt cagacccagt ggacacccca
     3961 acaagcacaa agcaacgacc caagagaagt atcaggaaag cagatgtaga gggagaactc
     4021 ttagcgtgca ggaatctaat gccatcagca ggcaaagcca tgcacacgcc taaaccatca
     4081 gtaggtgaag agaaagacat catcatattt gtgggaactc cagtgcagaa actggacctg
     4141 acagagaact taaccggcag caagagacgg ccacaaactc ctaaggaaga ggcccaggct
     4201 ctggaagacc tgactggctt taaagagctc ttccagaccc ctggtcatac tgaagaagca
     4261 gtggctgctg gcaaaactac taaaatgccc tgcgaatctt ctccaccaga atcagcagac
     4321 accccaacaa gcacaagaag gcagcccaag acacctttgg agaaaaggga cgtacagaag
     4381 gagctctcag ccctgaagaa gctcacacag acatcagggg aaaccacaca cacagataaa
     4441 gtaccaggag gtgaggataa aagcatcaac gcgtttaggg aaactgcaaa acagaaactg
     4501 gacccagcag caagtgtaac tggtagcaag aggcacccaa aaactaagga aaaggcccaa
     4561 cccctagaag acctggctgg cttgaaagag ctcttccaga caccagtatg cactgacaag
     4621 cccacgactc acgagaaaac taccaaaata gcctgcagat cacaaccaga cccagtggac
     4681 acaccaacaa gctccaagcc acagtccaag agaagtctca ggaaagtgga cgtagaagaa
     4741 gaattcttcg cactcaggaa acgaacacca tcagcaggca aagccatgca cacacccaaa
     4801 ccagcagtaa gtggtgagaa aaacatctac gcatttatgg gaactccagt gcagaaactg
     4861 gacctgacag agaacttaac tggcagcaag agacggctac aaactcctaa ggaaaaggcc
     4921 caggctctag aagacctggc tggctttaaa gagctcttcc agacacgagg tcacactgag
     4981 gaatcaatga ctaacgataa aactgccaaa gtagcctgca aatcttcaca accagaccca
     5041 gacaaaaacc cagcaagctc caagcgacgg ctcaagacat ccctggggaa agtgggcgtg
     5101 aaagaagagc tcctagcagt tggcaagctc acacagacat caggagagac tacacacaca
     5161 cacacagagc caacaggaga tggtaagagc atgaaagcat ttatggagtc tccaaagcag
     5221 atcttagact cagcagcaag tctaactggc agcaagaggc agctgagaac tcctaaggga
     5281 aagtctgaag tccctgaaga cctggccggc ttcatcgagc tcttccagac accaagtcac
     5341 actaaggaat caatgactaa tgaaaaaact accaaagtat cctacagagc ttcacagcca
     5401 gacctagtgg acaccccaac aagctccaag ccacagccca agagaagtct caggaaagca
     5461 gacactgaag aagaattttt agcatttagg aaacaaacgc catcagcagg caaagccatg
     5521 cacacaccca aaccagcagt aggtgaagag aaagacatca acacgttttt gggaactcca
     5581 gtgcagaaac tggaccagcc aggaaattta cctggcagca atagacggct acaaactcgt
     5641 aaggaaaagg cccaggctct agaagaactg actggcttca gagagctttt ccagacacca
     5701 tgcactgata accccacgac tgatgagaaa actaccaaaa aaatactctg caaatctccg
     5761 caatcagacc cagcggacac cccaacaaac acaaagcaac ggcccaagag aagcctcaag
     5821 aaagcagacg tagaggaaga atttttagca ttcaggaaac taacaccatc agcaggcaaa
     5881 gccatgcaca cgcctaaagc agcagtaggt gaagagaaag acatcaacac atttgtgggg
     5941 actccagtgg agaaactgga cctgctagga aatttacctg gcagcaagag acggccacaa
     6001 actcctaaag aaaaggccaa ggctctagaa gatctggctg gcttcaaaga gctcttccag
     6061 acaccaggtc acactgagga atcaatgacc gatgacaaaa tcacagaagt atcctgcaaa
     6121 tctccacaac cagacccagt caaaacccca acaagctcca agcaacgact caagatatcc
     6181 ttggggaaag taggtgtgaa agaagaggtc ctaccagtcg gcaagctcac acagacgtca
     6241 gggaagacca cacagacaca cagagagaca gcaggagatg gaaagagcat caaagcgttt
     6301 aaggaatctg caaagcagat gctggaccca gcaaactatg gaactgggat ggagaggtgg
     6361 ccaagaacac ctaaggaaga ggcccaatca ctagaagacc tggccggctt caaagagctc
     6421 ttccagacac cagaccacac tgaggaatca acaactgatg acaaaactac caaaatagcc
     6481 tgcaaatctc caccaccaga atcaatggac actccaacaa gcacaaggag gcggcccaaa
     6541 acacctttgg ggaaaaggga tatagtggaa gagctctcag ccctgaagca gctcacacag
     6601 accacacaca cagacaaagt accaggagat gaggataaag gcatcaacgt gttcagggaa
     6661 actgcaaaac agaaactgga cccagcagca agtgtaactg gtagcaagag gcagccaaga
     6721 actcctaagg gaaaagccca acccctagaa gacttggctg gcttgaaaga gctcttccag
     6781 acaccaatat gcactgacaa gcccacgact catgagaaaa ctaccaaaat agcctgcaga
     6841 tctccacaac cagacccagt gggtacccca acaatcttca agccacagtc caagagaagt
     6901 ctcaggaaag cagacgtaga ggaagaatcc ttagcactca ggaaacgaac accatcagta
     6961 gggaaagcta tggacacacc caaaccagca ggaggtgatg agaaagacat gaaagcattt
     7021 atgggaactc cagtgcagaa attggacctg ccaggaaatt tacctggcag caaaagatgg
     7081 ccacaaactc ctaaggaaaa ggcccaggct ctagaagacc tggctggctt caaagagctc
     7141 ttccagacac caggcactga caagcccacg actgatgaga aaactaccaa aatagcctgc
     7201 aaatctccac aaccagaccc agtggacacc ccagcaagca caaagcaacg gcccaagaga
     7261 aacctcagga aagcagacgt agaggaagaa tttttagcac tcaggaaacg aacaccatca
     7321 gcaggcaaag ccatggacac accaaaacca gcagtaagtg atgagaaaaa tatcaacaca
     7381 tttgtggaaa ctccagtgca gaaactggac ctgctaggaa atttacctgg cagcaagaga
     7441 cagccacaga ctcctaagga aaaggctgag gctctagagg acctggttgg cttcaaagaa
     7501 ctcttccaga caccaggtca cactgaggaa tcaatgactg atgacaaaat cacagaagta
     7561 tcctgtaaat ctccacagcc agagtcattc aaaacctcaa gaagctccaa gcaaaggctc
     7621 aagatacccc tggtgaaagt ggacatgaaa gaagagcccc tagcagtcag caagctcaca
     7681 cggacatcag gggagactac gcaaacacac acagagccaa caggagatag taagagcatc
     7741 aaagcgttta aggagtctcc aaagcagatc ctggacccag cagcaagtgt aactggtagc
     7801 aggaggcagc tgagaactcg taaggaaaag gcccgtgctc tagaagacct ggttgacttc
     7861 aaagagctct tctcagcacc aggtcacact gaagagtcaa tgactattga caaaaacaca
     7921 aaaattccct gcaaatctcc cccaccagaa ctaacagaca ctgccacgag cacaaagaga
     7981 tgccccaaga cacgtcccag gaaagaagta aaagaggagc tctcagcagt tgagaggctc
     8041 acgcaaacat cagggcaaag cacacacaca cacaaagaac cagcaagcgg tgatgagggc
     8101 atcaaagtat tgaagcaacg tgcaaagaag aaaccaaacc cagtagaaga ggaacccagc
     8161 aggagaaggc caagagcacc taaggaaaag gcccaacccc tggaagacct ggccggcttc
     8221 acagagctct ctgaaacatc aggtcacact caggaatcac tgactgctgg caaagccact
     8281 aaaataccct gcgaatctcc cccactagaa gtggtagaca ccacagcaag cacaaagagg
     8341 catctcagga cacgtgtgca gaaggtacaa gtaaaagaag agccttcagc agtcaagttc
     8401 acacaaacat caggggaaac cacggatgca gacaaagaac cagcaggtga agataaaggc
     8461 atcaaagcat tgaaggaatc tgcaaaacag acaccggctc cagcagcaag tgtaactggc
     8521 agcaggagac ggccaagagc acccagggaa agtgcccaag ccatagaaga cctagctggc
     8581 ttcaaagacc cagcagcagg tcacactgaa gaatcaatga ctgatgacaa aaccactaaa
     8641 ataccctgca aatcatcacc agaactagaa gacaccgcaa caagctcaaa gagacggccc
     8701 aggacacgtg cccagaaagt agaagtgaag gaggagctgt tagcagttgg caagctcaca
     8761 caaacctcag gggagaccac gcacaccgac aaagagccgg taggtgaggg caaaggcacg
     8821 aaagcattta agcaacctgc aaagcggaag ctggacgcag aagatgtaat tggcagcagg
     8881 agacagccaa gagcacctaa ggaaaaggcc caacccctgg aagatctggc cagcttccaa
     8941 gagctctctc aaacaccagg ccacactgag gaactggcaa atggtgctgc tgatagcttt
     9001 acaagcgctc caaagcaaac acctgacagt ggaaaacctc taaaaatatc cagaagagtt
     9061 cttcgggccc ctaaagtaga acccgtggga gacgtggtaa gcaccagaga ccctgtaaaa
     9121 tcacaaagca aaagcaacac ttccctgccc ccactgccct tcaagagggg aggtggcaaa
     9181 gatggaagcg tcacgggaac caagaggttg cgctgcatgc cagcaccaga ggaaattgtg
     9241 gaggagctgc cagccagcaa gaagcagagg gttgctccca gggcaagagg caaatcatcc
     9301 gaacccgtgg tcatcatgaa gagaagtttg aggacttctg caaaaagaat tgaacctgcg
     9361 gaagagctga acagcaacga catgaaaacc aacaaagagg aacacaaatt acaagactcg
     9421 gtccctgaaa ataagggaat atccctgcgc tccagacgcc aaaataagac tgaggcagaa
     9481 cagcaaataa ctgaggtctt tgtattagca gaaagaatag aaataaacag aaatgaaaag
     9541 aagcccatga agacctcccc agagatggac attcagaatc cagatgatgg agcccggaaa
     9601 cccataccta gagacaaagt cactgagaac aaaaggtgct tgaggtctgc tagacagaat
     9661 gagagctccc agcctaaggt ggcagaggag agcggagggc agaagagtgc gaaggttctc
     9721 atgcagaatc agaaagggaa aggagaagca ggaaattcag actccatgtg cctgagatca
     9781 agaaagacaa aaagccagcc tgcagcaagc actttggaga gcaaatctgt gcagagagta
     9841 acgcggagtg tcaagaggtg tgcagaaaat ccaaagaagg ctgaggacaa tgtgtgtgtc
     9901 aagaaaataa gaaccagaag tcatagggac agtgaagata tttgggatcc tatggtgagc
     9961 aagggcgagg aggataacat ggcctctctc ccagcgacac atgagttaca catctttggc
    10021 tccatcaacg gtgtggactt tgacatggtg ggtcagggca ccggcaatcc aaatgatggt
    10081 tatgaggagt taaacctgaa gtccaccaag ggtgacctcc agttctcccc ctggattctg
    10141 gtccctcata tcgggtatgg cttccatcag tacctgccct accctgacgg gatgtcgcct
    10201 ttccaggccg ccatggtaga tggctccgga taccaagtcc atcgcacaat gcagtttgaa
    10261 gatggtgcct cccttactgt taactaccgc tacacctacg agggaagcca catcaaagga
    10321 gaggcccagg tgaaggggac tggtttccct gctgacggtc ctgtgatgac caactcgctg
    10381 accgctgcgg actggtgcag gtcgaagaag acttacccca acgacaaaac catcatcagt
    10441 acctttaagt ggagttacac cactggaaat ggcaagcgct accggagcac tgcgcggacc
    10501 acctacacct ttgccaagcc aatggcggct aactatctga agaaccagcc gatgtacgtg
    10561 ttccgtaaga cggagctcaa gcactccaag accgagctca acttcaagga gtggcaaaag
    10621 gcctttaccg atgtgatggg catggacgag ctgtacaagt aagcggccgc atagataact
    10681 gatccagtgt gctggaatta attcgctgtc tgcgagggcc agctgttggg gtgagtactc
    10741 cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa acgaggagga
    10801 tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca tctggtcaga
    10861 aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct ggccatacac
    10921 ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
    10981 ctgcaggtcg agcatgcatc tagggcggcc aattccgccc ctctcccccc ccccctctcc
    11041 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
    11101 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
    11161 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
    11221 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
    11281 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
    11341 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
    11401 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
    11461 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
    11521 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
    11581 tttcctttga aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg
    11641 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
    11701 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
    11761 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
    11821 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
    11881 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
    11941 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
    12001 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
    12061 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
    12121 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
    12181 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
    12241 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga
    12301 agccacccgg ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagag
    12361 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
    12421 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
    12481 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
    12541 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
    12601 tggcttctga ggcggaaaga accagctggg gctcgagtgc attctagttg tggtttgtcc
    12661 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
    12721 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    12781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    12841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    12901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    12961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    13021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    13081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    13141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    13201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    13261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    13321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    13381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    13441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    13501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    13561 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    13621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    13681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    13741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    13801 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    13861 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    13921 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    13981 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    14041 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    14101 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    14161 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    14221 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    14281 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    14341 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    14401 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    14461 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    14521 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    14581 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    14641 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    14701 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    14761 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    14821 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    14881 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
    14941 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
    15001 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
    15061 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
    15121 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
    15181 catatatgga gttc
//
