LOCUS       sequence-367958-        9752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ki67∆repeats-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: Ki67∆repeats-mNeonGreen-IRESpuro2
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     461..481
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4510..5220
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4765..4774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5289..5518
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5501..5520
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5590..6164,6165..6167,6168..6176)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5757..5774)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5984..6003
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6196..6795
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6196..6215)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6692..6712
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6931..6948)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6937..7161
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7208..7227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7287..7303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7287..7303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7300..7322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7311..7327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7335..7341,7342..7359,7360..7365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7380..7401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7518..7535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7689..8277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7769..7788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8448..9239,9240..9308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9071..9090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9309..9413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9488..9507)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9679..9752,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaatgtggc ccacgagacg cctggttact
      601 atcaaaagga gcggggtcga cggtccccac tttcccctga gcctcagcac ctgcttgttt
      661 ggaaggggta ttgaatgtga catccgtatc cagcttcctg ttgtgtcaaa acaacattgc
      721 aaaattgaaa tccatgagca ggaggcaata ttacataatt tcagttccac aaatccaaca
      781 caagtaaatg ggtctgttat tgatgagcct gtacggctaa aacatggaga tgtaataact
      841 attattgatc gttccttcag gtatgaaaat gaaagtcttc agaatggaag gaagtcaact
      901 gaatttccaa gaaaaatacg tgaacaggag ccagcacgtc gtgtctcaag atctagcttc
      961 tcttctgacc ctgatgagaa agctcaagat tccaaggcct attcaaaaat cactgaagga
     1021 aaagtttcag gaaatcctca ggtacatatc aagaatgtca aagaagacag taccgcagat
     1081 gactcaaaag acagtgttgc tcagggaaca actaatgttc attcctcaga acatgctgga
     1141 cgtaatggca gaaatgcagc tgatcccatt tctggggatt ttaaagaaat ttccagcgtt
     1201 aaattagtga gccgttatgg agaattgaag tctgttccca ctacacaatg tcttgacaat
     1261 agcaaaaaaa atgaatctcc cttttggaag ctttatgagt cagtgaagaa agagttggat
     1321 gtaaaatcac aaaaagaaaa tgtcctacag tattgtagaa aatctggatt acaaactgat
     1381 tacgcaacag agaaagaaag tgctgatggt ttacaggggg agacccaact gttggtctcg
     1441 cgtaagtcaa gaccaaaatc tggtgggagc ggccacgctg tggcagagcc tgcttcacct
     1501 gaacaagagc ttgaccagaa caaggggaag ggaagagacg tggagtctgt tcagactccc
     1561 agcaaggctg tgggcgccag ctttcctctc tatgagccgg ctaaaatgaa gacccctgta
     1621 caatattcac agcaacaaaa ttctccacaa aaacataaga acaaagacct gtatactact
     1681 ggtagaagag aatctgtgaa tctgggtaaa agtgaaggct tcaaggctgg tgataaaact
     1741 cttactccca ggaagctttc aactagaaat cgaacaccag ctaaagttga agatgcagct
     1801 gactctgcca ctaagccaga aaatctctct tccaaaacca gaggaagtat tcctacagat
     1861 gtggaagttc tgcctacgga aactgaaatt cacaatgagc catttttaac tctgtggctc
     1921 actcaagttg agaggaagat ccaaaaggat tccctcagca agcctgagaa attgggcact
     1981 acagctggac agatgtgctc tgggttacct ggtcttagtt cagttgatat caacaacttt
     2041 ggtgattcca ttaatgagag tgagggaata cctttgaaaa gaaggcgtgt gtcctttggt
     2101 gggcacctaa gacctgaact atttgatgaa aacttgcctc ctaatacgcc tctcaaaagg
     2161 ggagaagccc caaccaaaag aaagtctctg gtaatgcaca ctccacctgt cctgaagaaa
     2221 atcatcaagg aacagcctca accatcagga aaacaagagt caggttcaga aatccatgtg
     2281 gaagtgaagg cacaaagctt ggttataagc cctccagctc ctagtcctag gaaaactcca
     2341 gttgccagtg atcaacgccg taggtcctgc aaaacagccc ctgcttccag cagcaaatct
     2401 cagacagagg ttcctaagag aggagggaga aagagtggca acctgccttc aaagagagtg
     2461 tctatcagcc gaagtcaaca tgatatttta cagatgatat gttccaaaag aagaagtggt
     2521 gcttcggaag caaatctgat tgttgcaaaa tcatgggcag atgtagtaaa acttggtgca
     2581 aaacaaacac aaactaaagt cataaaacat ggtcctcaaa ggtcaatgaa caaaaggcaa
     2641 agaagacctg ctactccaaa gaagcctgtg ggcgaagttc acagtcaatt tagtacaggc
     2701 cacgcaaact ctccttgtac cataataata gggaaagctc atactgaaaa agtacatgtg
     2761 cctgctcgac cctacagagt gctcaacaac ttcatttcca accaaaaaat ggactttaag
     2821 gaagatcttt caggaatagc tgaaatgttc aagaccccag tgaaggagca accgcagttg
     2881 acaagcacat gtcacatcgc tatttcaaat tcagagaatt tgcttggaaa acagtttcaa
     2941 ggaactgatt caggagaaga acctctgctc cccacctcag agagttttgg aggaaatgtg
     3001 ttcttcagtg cacagaatgc agcaaaacag ccatctgata aatgctctgc aagccctccc
     3061 ttaagacggc agtgtattag agaaaatgga aacgtagcaa aaacgcccag gaacacctac
     3121 aaaatgactt ctctggagac aaaaacttca gatactgaga cagagccttc aaaaacagta
     3181 tccactgcaa acaggtcagg aaggtctaca gagttcagga atatacagaa gctacctgtg
     3241 gaaagtaaga gtgaagaaac aaatacagaa attgttgagt gcatcctaaa aagaggtcag
     3301 aaggcaacac tactacaaca aaggagagaa ggagagatga aggaaataga aagacctttt
     3361 gagacatata aggaaaatat tgaattaaaa gaaaacgatg aaaagatgaa agcaatgaag
     3421 agatcaagaa cttgggggca gaaatgtgca ccaatgtctg acctgacaga cctcaagagc
     3481 ttgcctgata cagaactcat gaaagacacg gcacgtggcc agaatctcct ccaaacccaa
     3541 gatcatgcca aggcacaccg gtgcgctcca aagcaaacac ctgacagtgg aaaacctcta
     3601 aaaatatcca gaagagttct tcgggcccct aaagtagaac ccgtgggaga cgtggtaagc
     3661 accagagacc ctgtaaaatc acaaagcaaa agcaacactt ccctgccccc actgcccttc
     3721 aagaggggag gtggcaaaga tggaagcgtc acgggaacca agaggttgcg ctgcatgcca
     3781 gcaccagagg aaattgtgga ggagctgcca gccagcaaga agcagagggt tgctcccagg
     3841 gcaagaggca aatcatccga acccgtggtc atcatgaaga gaagtttgag gacttctgca
     3901 aaaagaattg aacctgcgga agagctgaac agcaacgaca tgaaaaccaa caaagaggaa
     3961 cacaaattac aagactcggt ccctgaaaat aagggaatat ccctgcgctc cagacgccaa
     4021 aataagactg aggcagaaca gcaaataact gaggtctttg tattagcaga aagaatagaa
     4081 ataaacagaa atgaaaagaa gcccatgaag acctccccag agatggacat tcagaatcca
     4141 gatgatggag cccggaaacc catacctaga gacaaagtca ctgagaacaa aaggtgcttg
     4201 aggtctgcta gacagaatga gagctcccag cctaaggtgg cagaggagag cggagggcag
     4261 aagagtgcga aggttctcat gcagaatcag aaagggaaag gagaagcagg aaattcagac
     4321 tccatgtgcc tgagatcaag aaagacaaaa agccagcctg cagcaagcac tttggagagc
     4381 aaatctgtgc agagagtaac gcggagtgtc aagaggtgtg cagaaaatcc aaagaaggct
     4441 gaggacaatg tgtgtgtcaa gaaaataaga accagaagtc atagggacag tgaagatatt
     4501 tgggatccta tggtgagcaa gggcgaggag gataacatgg cctctctccc agcgacacat
     4561 gagttacaca tctttggctc catcaacggt gtggactttg acatggtggg tcagggcacc
     4621 ggcaatccaa atgatggtta tgaggagtta aacctgaagt ccaccaaggg tgacctccag
     4681 ttctccccct ggattctggt ccctcatatc gggtatggct tccatcagta cctgccctac
     4741 cctgacggga tgtcgccttt ccaggccgcc atggtagatg gctccggata ccaagtccat
     4801 cgcacaatgc agtttgaaga tggtgcctcc cttactgtta actaccgcta cacctacgag
     4861 ggaagccaca tcaaaggaga ggcccaggtg aaggggactg gtttccctgc tgacggtcct
     4921 gtgatgacca actcgctgac cgctgcggac tggtgcaggt cgaagaagac ttaccccaac
     4981 gacaaaacca tcatcagtac ctttaagtgg agttacacca ctggaaatgg caagcgctac
     5041 cggagcactg cgcggaccac ctacaccttt gccaagccaa tggcggctaa ctatctgaag
     5101 aaccagccga tgtacgtgtt ccgtaagacg gagctcaagc actccaagac cgagctcaac
     5161 ttcaaggagt ggcaaaaggc ctttaccgat gtgatgggca tggacgagct gtacaagtaa
     5221 gcggccgcat agataactga tccagtgtgc tggaattaat tcgctgtctg cgagggccag
     5281 ctgttggggt gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt
     5341 ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc
     5401 cgcgtccatc tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct
     5461 tgagatctgg ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt
     5521 gtccactccc aggtccaact gcaggtcgag catgcatcta gggcggccaa ttccgcccct
     5581 ctcccccccc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     5641 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     5701 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     5761 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5821 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5881 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5941 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6001 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6061 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6121 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca
     6181 aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6241 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6301 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6361 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6421 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6481 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6541 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6601 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6661 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6721 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6781 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     6841 ccccatggct ccgaccgaag ccacccgggg cggccccgcc gaccccgcac ccgcccccga
     6901 ggcccaccga ctctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     6961 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7021 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7081 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7141 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgagtgcat
     7201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7261 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7321 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7381 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7441 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7501 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8101 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9421 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     9481 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9541 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     9601 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     9661 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     9721 ggtcattagt tcatagccca tatatggagt tc
//
