LOCUS       sequence-367959-       16370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16370)
  AUTHORS   Cuylen-Haering S, Petrovic M, Hernandez-Armendariz A, Schneider MWG,
            Samwer M, Blaukopf C, Holt LJ, Gerlich DW
  TITLE     Chromosome clustering by Ki-67 excludes cytoplasm during nuclear 
            assembly.
  JOURNAL   Nature. 2020 Nov;587(7833):285-290. doi: 10.1038/s41586-020-2672-3. 
            Epub 2020 Sep 2.
  PUBMED    32879492
REFERENCE   2  (bases 1 to 16370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2672-3"; journalName: "Nature"; date: "2020-11";
            volume: "587"; issue: "7833"; pages: "285-290"
COMMENT     Sequence Label: EGFP-Ki-67(RASA)-mCherry_IRESpuro2
FEATURES             Location/Qualifiers
     source          1..16370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..714)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(43..64)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(304..323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             4310..4321
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4676..4687
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5402..5413
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6134..6145
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7586..7597
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             10540..11250
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10685..10703)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10953..10972
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          11320..11549
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     11532..11551
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(11621..12195,12196..12198,12199..12207)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11788..11805)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12015..12034
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12227..12826
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12227..12246)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12723..12743
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(12962..12979)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12968..13192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13239..13258
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13318..13334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13318..13334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13331..13353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13342..13358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13366..13372,13373..13390,13391..13396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13411..13432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13549..13566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13720..14308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13800..13819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14479..15270,15271..15339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15102..15121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15340..15444)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15519..15538)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        15710..16089
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16090..16293
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16244..16264
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
       61 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      181 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      421 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      481 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      601 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctt
      661 aaggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcagc
      721 ggccccagcg gcagcagcag ctggcccacg agacgcctgg ttactatcaa aaggagcggg
      781 gtcgacggtc cccactttcc cctgagcctc agcacctgct tgtttggaag gggtattgaa
      841 tgtgacatcc gtatccagct tcctgttgtg tcaaaacaac attgcaaaat tgaaatccat
      901 gagcaggagg caatattaca taatttcagt tccacaaatc caacacaagt aaatgggtct
      961 gttattgatg agcctgtacg gctaaaacat ggagatgtaa taactattat tgatcgttcc
     1021 ttcaggtatg aaaatgaaag tcttcagaat ggaaggaagt caactgaatt tccaagaaaa
     1081 atacgtgaac aggagccagc acgtcgtgtc tcaagatcta gcttctcttc tgaccctgat
     1141 gagaaagctc aagattccaa ggcctattca aaaatcactg aaggaaaagt ttcaggaaat
     1201 cctcaggtac atatcaagaa tgtcaaagaa gacagtaccg cagatgactc aaaagacagt
     1261 gttgctcagg gaacaactaa tgttcattcc tcagaacatg ctggacgtaa tggcagaaat
     1321 gcagctgatc ccatttctgg ggattttaaa gaaatttcca gcgttaaatt agtgagccgt
     1381 tatggagaat tgaagtctgt tcccactaca caatgtcttg acaatagcaa aaaaaatgaa
     1441 tctccctttt ggaagcttta tgagtcagtg aagaaagagt tggatgtaaa atcacaaaaa
     1501 gaaaatgtcc tacagtattg tagaaaatct ggattacaaa ctgattacgc aacagagaaa
     1561 gaaagtgctg atggtttaca gggggagacc caactgttgg tctcgcgtaa gtcaagacca
     1621 aaatctggtg ggagcggcca cgctgtggca gagcctgctt cacctgaaca agagcttgac
     1681 cagaacaagg ggaagggaag agacgtggag tctgttcaga ctcccagcaa ggctgtgggc
     1741 gccagctttc ctctctatga gccggctaaa atgaagaccc ctgtacaata ttcacagcaa
     1801 caaaattctc cacaaaaaca taagaacaaa gacctgtata ctactggtag aagagaatct
     1861 gtgaatctgg gtaaaagtga aggcttcaag gctggtgata aaactcttac tcccaggaag
     1921 ctttcaacta gaaatcgaac accagctaaa gttgaagatg cagctgactc tgccactaag
     1981 ccagaaaatc tctcttccaa aaccagagga agtattccta cagatgtgga agttctgcct
     2041 acggaaactg aaattcacaa tgagccattt ttaactctgt ggctcactca agttgagagg
     2101 aagatccaaa aggattccct cagcaagcct gagaaattgg gcactacagc tggacagatg
     2161 tgctctgggt tacctggtct tagttcagtt gatatcaaca actttggtga ttccattaat
     2221 gagagtgagg gaataccttt gaaaagaagg cgtgcctccg ctggtgggca cctaagacct
     2281 gaactatttg atgaaaactt gcctcctaat acgcctctca aaaggggaga agccccaacc
     2341 aaaagaaagt ctctggtaat gcacactcca cctgtcctga agaaaatcat caaggaacag
     2401 cctcaaccat caggaaaaca agagtcaggt tcagaaatcc atgtggaagt gaaggcacaa
     2461 agcttggtta taagccctcc agctcctagt cctaggaaaa ctccagttgc cagtgatcaa
     2521 cgccgtaggt cctgcaaaac agcccctgct tccagcagca aatctcagac agaggttcct
     2581 aagagaggag ggagaaagag tggcaacctg ccttcaaaga gagtgtctat cagccgaagt
     2641 caacatgata ttttacagat gatatgttcc aaaagaagaa gtggtgcttc ggaagcaaat
     2701 ctgattgttg caaaatcatg ggcagatgta gtaaaacttg gtgcaaaaca aacacaaact
     2761 aaagtcataa aacatggtcc tcaaaggtca atgaacaaaa ggcaaagaag acctgctact
     2821 ccaaagaagc ctgtgggcga agttcacagt caatttagta caggccacgc aaactctcct
     2881 tgtaccataa taatagggaa agctcatact gaaaaagtac atgtgcctgc tcgaccctac
     2941 agagtgctca acaacttcat ttccaaccaa aaaatggact ttaaggaaga tctttcagga
     3001 atagctgaaa tgttcaagac cccagtgaag gagcaaccgc agttgacaag cacatgtcac
     3061 atcgctattt caaattcaga gaatttgctt ggaaaacagt ttcaaggaac tgattcagga
     3121 gaagaacctc tgctccccac ctcagagagt tttggaggaa atgtgttctt cagtgcacag
     3181 aatgcagcaa aacagccatc tgataaatgc tctgcaagcc ctcccttaag acggcagtgt
     3241 attagagaaa atggaaacgt agcaaaaacg cccaggaaca cctacaaaat gacttctctg
     3301 gagacaaaaa cttcagatac tgagacagag ccttcaaaaa cagtatccac tgcaaacagg
     3361 tcaggaaggt ctacagagtt caggaatata cagaagctac ctgtggaaag taagagtgaa
     3421 gaaacaaata cagaaattgt tgagtgcatc ctaaaaagag gtcagaaggc aacactacta
     3481 caacaaagga gagaaggaga gatgaaggaa atagaaagac cttttgagac atataaggaa
     3541 aatattgaat taaaagaaaa cgatgaaaag atgaaagcaa tgaagagatc aagaacttgg
     3601 gggcagaaat gtgcaccaat gtctgacctg acagacctca agagcttgcc tgatacagaa
     3661 ctcatgaaag acacggcacg tggccagaat ctcctccaaa cccaagatca tgccaaggca
     3721 ccaaagagtg agaaaggcaa aatcactaaa atgccctgcc agtcattaca accagaacca
     3781 ataaacaccc caacacacac aaaacaacag ttgaaggcat ccctggggaa agtaggtgtg
     3841 aaagaagagc tcctagcagt cggcaagttc acacggacgt caggggagac cacgcacacg
     3901 cacagagagc cagcaggaga tggcaagagc atcagaacgt ttaaggagtc tccaaagcag
     3961 atcctggacc cagcagcccg tgtaactgga atgaagaagt ggccaagaac gcctaaggaa
     4021 gaggcccagt cactagaaga cctggctggc ttcaaagagc tcttccagac accaggtccc
     4081 tctgaggaat caatgactga tgagaaaact accaaaatag cctgcaaatc tccaccacca
     4141 gaatcagtgg acactccaac aagcacaaag caatggccta agagaagtct caggaaagca
     4201 gatgtagagg aagaattctt agcactcagg aaactaacac catcagcagg gaaagccatg
     4261 cttacgccca aaccagcagg aggtgatgag aaagacatta aagcatttat gggaactcca
     4321 gtgcagaaac tggacctggc aggaacttta cctggcagca aaagacagct acagactcct
     4381 aaggaaaagg cccaggctct agaagacctg gctggcttta aagagctctt ccagactcct
     4441 ggtcacaccg aggaattagt ggctgctggt aaaaccacta aaataccctg cgactctcca
     4501 cagtcagacc cagtggacac cccaacaagc acaaagcaac gacccaagag aagtatcagg
     4561 aaagcagatg tagagggaga actcttagcg tgcaggaatc taatgccatc agcaggcaaa
     4621 gccatgcaca cgcctaaacc atcagtaggt gaagagaaag acatcatcat atttgtggga
     4681 actccagtgc agaaactgga cctgacagag aacttaaccg gcagcaagag acggccacaa
     4741 actcctaagg aagaggccca ggctctggaa gacctgactg gctttaaaga gctcttccag
     4801 acccctggtc atactgaaga agcagtggct gctggcaaaa ctactaaaat gccctgcgaa
     4861 tcttctccac cagaatcagc agacacccca acaagcacaa gaaggcagcc caagacacct
     4921 ttggagaaaa gggacgtaca gaaggagctc tcagccctga agaagctcac acagacatca
     4981 ggggaaacca cacacacaga taaagtacca ggaggtgagg ataaaagcat caacgcgttt
     5041 agggaaactg caaaacagaa actggaccca gcagcaagtg taactggtag caagaggcac
     5101 ccaaaaacta aggaaaaggc ccaaccccta gaagacctgg ctggcttgaa agagctcttc
     5161 cagacaccag tatgcactga caagcccacg actcacgaga aaactaccaa aatagcctgc
     5221 agatcacaac cagacccagt ggacacacca acaagctcca agccacagtc caagagaagt
     5281 ctcaggaaag tggacgtaga agaagaattc ttcgcactca ggaaacgaac accatcagca
     5341 ggcaaagcca tgcacacacc caaaccagca gtaagtggtg agaaaaacat ctacgcattt
     5401 atgggaactc cagtgcagaa actggacctg acagagaact taactggcag caagagacgg
     5461 ctacaaactc ctaaggaaaa ggcccaggct ctagaagacc tggctggctt taaagagctc
     5521 ttccagacac gaggtcacac tgaggaatca atgactaacg ataaaactgc caaagtagcc
     5581 tgcaaatctt cacaaccaga cccagacaaa aacccagcaa gctccaagcg acggctcaag
     5641 acatccctgg ggaaagtggg cgtgaaagaa gagctcctag cagttggcaa gctcacacag
     5701 acatcaggag agactacaca cacacacaca gagccaacag gagatggtaa gagcatgaaa
     5761 gcatttatgg agtctccaaa gcagatctta gactcagcag caagtctaac tggcagcaag
     5821 aggcagctga gaactcctaa gggaaagtct gaagtccctg aagacctggc cggcttcatc
     5881 gagctcttcc agacaccaag tcacactaag gaatcaatga ctaatgaaaa aactaccaaa
     5941 gtatcctaca gagcttcaca gccagaccta gtggacaccc caacaagctc caagccacag
     6001 cccaagagaa gtctcaggaa agcagacact gaagaagaat ttttagcatt taggaaacaa
     6061 acgccatcag caggcaaagc catgcacaca cccaaaccag cagtaggtga agagaaagac
     6121 atcaacacgt ttttgggaac tccagtgcag aaactggacc agccaggaaa tttacctggc
     6181 agcaatagac ggctacaaac tcgtaaggaa aaggcccagg ctctagaaga actgactggc
     6241 ttcagagagc ttttccagac accatgcact gataacccca cgactgatga gaaaactacc
     6301 aaaaaaatac tctgcaaatc tccgcaatca gacccagcgg acaccccaac aaacacaaag
     6361 caacggccca agagaagcct caagaaagca gacgtagagg aagaattttt agcattcagg
     6421 aaactaacac catcagcagg caaagccatg cacacgccta aagcagcagt aggtgaagag
     6481 aaagacatca acacatttgt ggggactcca gtggagaaac tggacctgct aggaaattta
     6541 cctggcagca agagacggcc acaaactcct aaagaaaagg ccaaggctct agaagatctg
     6601 gctggcttca aagagctctt ccagacacca ggtcacactg aggaatcaat gaccgatgac
     6661 aaaatcacag aagtatcctg caaatctcca caaccagacc cagtcaaaac cccaacaagc
     6721 tccaagcaac gactcaagat atccttgggg aaagtaggtg tgaaagaaga ggtcctacca
     6781 gtcggcaagc tcacacagac gtcagggaag accacacaga cacacagaga gacagcagga
     6841 gatggaaaga gcatcaaagc gtttaaggaa tctgcaaagc agatgctgga cccagcaaac
     6901 tatggaactg ggatggagag gtggccaaga acacctaagg aagaggccca atcactagaa
     6961 gacctggccg gcttcaaaga gctcttccag acaccagacc acactgagga atcaacaact
     7021 gatgacaaaa ctaccaaaat agcctgcaaa tctccaccac cagaatcaat ggacactcca
     7081 acaagcacaa ggaggcggcc caaaacacct ttggggaaaa gggatatagt ggaagagctc
     7141 tcagccctga agcagctcac acagaccaca cacacagaca aagtaccagg agatgaggat
     7201 aaaggcatca acgtgttcag ggaaactgca aaacagaaac tggacccagc agcaagtgta
     7261 actggtagca agaggcagcc aagaactcct aagggaaaag cccaacccct agaagacttg
     7321 gctggcttga aagagctctt ccagacacca atatgcactg acaagcccac gactcatgag
     7381 aaaactacca aaatagcctg cagatctcca caaccagacc cagtgggtac cccaacaatc
     7441 ttcaagccac agtccaagag aagtctcagg aaagcagacg tagaggaaga atccttagca
     7501 ctcaggaaac gaacaccatc agtagggaaa gctatggaca cacccaaacc agcaggaggt
     7561 gatgagaaag acatgaaagc atttatggga actccagtgc agaaattgga cctgccagga
     7621 aatttacctg gcagcaaaag atggccacaa actcctaagg aaaaggccca ggctctagaa
     7681 gacctggctg gcttcaaaga gctcttccag acaccaggca ctgacaagcc cacgactgat
     7741 gagaaaacta ccaaaatagc ctgcaaatct ccacaaccag acccagtgga caccccagca
     7801 agcacaaagc aacggcccaa gagaaacctc aggaaagcag acgtagagga agaattttta
     7861 gcactcagga aacgaacacc atcagcaggc aaagccatgg acacaccaaa accagcagta
     7921 agtgatgaga aaaatatcaa cacatttgtg gaaactccag tgcagaaact ggacctgcta
     7981 ggaaatttac ctggcagcaa gagacagcca cagactccta aggaaaaggc tgaggctcta
     8041 gaggacctgg ttggcttcaa agaactcttc cagacaccag gtcacactga ggaatcaatg
     8101 actgatgaca aaatcacaga agtatcctgt aaatctccac agccagagtc attcaaaacc
     8161 tcaagaagct ccaagcaaag gctcaagata cccctggtga aagtggacat gaaagaagag
     8221 cccctagcag tcagcaagct cacacggaca tcaggggaga ctacgcaaac acacacagag
     8281 ccaacaggag atagtaagag catcaaagcg tttaaggagt ctccaaagca gatcctggac
     8341 ccagcagcaa gtgtaactgg tagcaggagg cagctgagaa ctcgtaagga aaaggcccgt
     8401 gctctagaag acctggttga cttcaaagag ctcttctcag caccaggtca cactgaagag
     8461 tcaatgacta ttgacaaaaa cacaaaaatt ccctgcaaat ctcccccacc agaactaaca
     8521 gacactgcca cgagcacaaa gagatgcccc aagacacgtc ccaggaaaga agtaaaagag
     8581 gagctctcag cagttgagag gctcacgcaa acatcagggc aaagcacaca cacacacaaa
     8641 gaaccagcaa gcggtgatga gggcatcaaa gtattgaagc aacgtgcaaa gaagaaacca
     8701 aacccagtag aagaggaacc cagcaggaga aggccaagag cacctaagga aaaggcccaa
     8761 cccctggaag acctggccgg cttcacagag ctctctgaaa catcaggtca cactcaggaa
     8821 tcactgactg ctggcaaagc cactaaaata ccctgcgaat ctcccccact agaagtggta
     8881 gacaccacag caagcacaaa gaggcatctc aggacacgtg tgcagaaggt acaagtaaaa
     8941 gaagagcctt cagcagtcaa gttcacacaa acatcagggg aaaccacgga tgcagacaaa
     9001 gaaccagcag gtgaagataa aggcatcaaa gcattgaagg aatctgcaaa acagacaccg
     9061 gctccagcag caagtgtaac tggcagcagg agacggccaa gagcacccag ggaaagtgcc
     9121 caagccatag aagacctagc tggcttcaaa gacccagcag caggtcacac tgaagaatca
     9181 atgactgatg acaaaaccac taaaataccc tgcaaatcat caccagaact agaagacacc
     9241 gcaacaagct caaagagacg gcccaggaca cgtgcccaga aagtagaagt gaaggaggag
     9301 ctgttagcag ttggcaagct cacacaaacc tcaggggaga ccacgcacac cgacaaagag
     9361 ccggtaggtg agggcaaagg cacgaaagca tttaagcaac ctgcaaagcg gaagctggac
     9421 gcagaagatg taattggcag caggagacag ccaagagcac ctaaggaaaa ggcccaaccc
     9481 ctggaagatc tggccagctt ccaagagctc tctcaaacac caggccacac tgaggaactg
     9541 gcaaatggtg ctgctgatag ctttacaagc gctccaaagc aaacacctga cagtggaaaa
     9601 cctctaaaaa tatccagaag agttcttcgg gcccctaaag tagaacccgt gggagacgtg
     9661 gtaagcacca gagaccctgt aaaatcacaa agcaaaagca acacttccct gcccccactg
     9721 cccttcaaga ggggaggtgg caaagatgga agcgtcacgg gaaccaagag gttgcgctgc
     9781 atgccagcac cagaggaaat tgtggaggag ctgccagcca gcaagaagca gagggttgct
     9841 cccagggcaa gaggcaaatc atccgaaccc gtggtcatca tgaagagaag tttgaggact
     9901 tctgcaaaaa gaattgaacc tgcggaagag ctgaacagca acgacatgaa aaccaacaaa
     9961 gaggaacaca aattacaaga ctcggtccct gaaaataagg gaatatccct gcgctccaga
    10021 cgccaaaata agactgaggc agaacagcaa ataactgagg tctttgtatt agcagaaaga
    10081 atagaaataa acagaaatga aaagaagccc atgaagacct ccccagagat ggacattcag
    10141 aatccagatg atggagcccg gaaacccata cctagagaca aagtcactga gaacaaaagg
    10201 tgcttgaggt ctgctagaca gaatgagagc tcccagccta aggtggcaga ggagagcgga
    10261 gggcagaaga gtgcgaaggt tctcatgcag aatcagaaag ggaaaggaga agcaggaaat
    10321 tcagactcca tgtgcctgag atcaagaaag acaaaaagcc agcctgcagc aagcactttg
    10381 gagagcaaat ctgtgcagag agtaacgcgg agtgtcaaga ggtgtgcaga aaatccaaag
    10441 aaggctgagg acaatgtgtg tgtcaagaaa ataagaacca gaagtcatag ggacagtgaa
    10501 gatatttggg atccatcgtc gagcggtccc tcgggttcga tggtgagcaa gggcgaggag
    10561 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
    10621 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
    10681 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
    10741 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
    10801 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
    10861 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
    10921 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
    10981 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
    11041 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
    11101 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
    11161 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
    11221 accggcggca tggacgagct gtacaagtaa agcggccgca tagataactg atccagtgtg
    11281 ctggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctcaaaag
    11341 cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
    11401 cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa aagacaatct
    11461 ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact tgagtgacaa
    11521 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcaggtcga
    11581 gcatgcatct agggcggcca attccgcccc tctccccccc cccctctccc tccccccccc
    11641 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
    11701 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
    11761 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
    11821 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
    11881 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
    11941 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
    12001 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
    12061 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
    12121 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
    12181 aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga ccgagtacaa
    12241 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
    12301 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
    12361 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
    12421 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
    12481 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
    12541 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
    12601 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
    12661 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
    12721 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
    12781 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc cgccccacga
    12841 cccgcagcgc ccgaccgaaa ggagcgcacg accccatggc tccgaccgaa gccacccggg
    12901 gcggccccgc cgaccccgca cccgcccccg aggcccaccg actctagagc tcgctgatca
    12961 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
    13021 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
    13081 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
    13141 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
    13201 gcggaaagaa ccagctgggg ctcgagtgca ttctagttgt ggtttgtcca aactcatcaa
    13261 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    13321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    13381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    13441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    13501 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    13561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    13621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    13681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    13741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    13801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    13861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    13921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    13981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    14041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    14101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    14161 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    14221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    14281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    14341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    14401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    14461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    14521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    14581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    14641 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    14701 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    14761 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    14821 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    14881 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    14941 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    15001 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    15061 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    15121 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    15181 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    15241 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    15301 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    15361 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    15421 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    15481 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    15541 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    15601 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    15661 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
    15721 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    15781 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    15841 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    15901 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    15961 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    16021 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    16081 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    16141 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
    16201 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    16261 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
    16321 cgctaccgga ctcagatctc gagctcaagc ttcgaaccgc catggctagc
//
