LOCUS       sequence-367968-       10238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FLAG epitope and APEX2-tagged NPAT fusion protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Barutcu AR, Wu M, Braunschweig U, Dyakov BJA, Luo Z, Turner KM, 
            Durbic T, Lin ZY, Weatheritt RJ, Maass PG, Gingras AC, Blencowe BJ
  TITLE     Systematic mapping of nuclear domain-associated transcripts reveals 
            speckles and lamina as hubs of functionally distinct retained 
            introns.
  JOURNAL   Mol Cell. 2022 Mar 3;82(5):1035-1052.e9. doi: 
            10.1016/j.molcel.2021.12.010. Epub 2022 Feb 18.
  PUBMED    35182477
REFERENCE   2  (bases 1 to 10238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.12.010"; journalName: "Mol Cell"; date: 
            "2022-03-3- 3"; volume: "82"; issue: "5"; pages: "1035-1052.e9"
COMMENT     Sequence Label: pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT
FEATURES             Location/Qualifiers
     source          1..10238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    987..1011
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5302..5326)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5337..6083
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             6084..6149
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6190..6207)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6196..6420
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6553..6572)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6704..6751
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6759..7781
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7911..8032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7948..7967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8002..8021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8081..8097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8081..8097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8094..8116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8105..8121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8129..8135,8136..8153,8154..8159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8174..8195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8312..8329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8483..9071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8563..8582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9242..10033,10034..10102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9865..9884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10103..10207)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag ctttagacaa gtttgtacaa aaaagcaggc tccaccatgt
     1021 tgttaccctc cgacgtagcc cggcttgtct tgggttactt acagcaagaa aacctcattt
     1081 ctacctgcca gacttttatt ttggaaagct cagatttaaa agaatatgca gaacattgta
     1141 cagatgaagg gtttattcca gcctgcttac tgtccttatt tggaaaaaac ttgacaacaa
     1201 ttttaaatga gtatgtggct atgaaaacaa aagaaacatc aaataatgtg cccgcaataa
     1261 tgtcatctct atggaagaaa ttggaccata cactttctca gatcaggagc atgcaaagtt
     1321 ccccaaggtt tgctggcagt cagagagccc gaacgagaac tggaattgca gaaatcaaac
     1381 ggcagagaaa gctggcatct caaacagctc ccgccagtgc agagttgctc actttacctt
     1441 acctttcagg acagtttacc actccacctt ccacaggaac acaggttact cgaccaagtg
     1501 gccaaatttc agatccatcc aggtcatatt ttgtagtggt caaccactca cagtcacaag
     1561 atactgtaac cactggagaa gctttaaatg tcattcctgg tgcgcaggaa aagaaagcac
     1621 atgctagttt aatgtctccc ggtcgacgca aaagtgaatc tcagagaaaa agtaccactt
     1681 tgtccggccc tcattcaaca atacggaatt tccaagatcc aaacgctttt gcagtagaaa
     1741 agcaaatggt tattgaaaac gcaagggaaa aaatactaag caacaaatct cttcaggaaa
     1801 agctagcaga aaacataaat aaatttttga ctagtgacaa caatattgcc caagtaccta
     1861 agcaaacaga taacaaccct accgagccag agacttcaat tgatgaattc ctaggacttc
     1921 cgagtgaaat tcacatgtct gaagaagcta tacaggacat attggaacag acagaatcag
     1981 acccagcatt tcaggcactc tttgatctct ttgactatgg aaaaacaaag aataataaaa
     2041 atatatccca aagtatttcc agtcaaccta tggaatccaa tcccagtata gtcttagcag
     2101 atgaaactaa tctagcagtt aaaggttctt ttgaaacaga agaatcagat ggtcagtctg
     2161 gtcagcccgc cttttgtaca tcctatcaga atgatgaccc attaaatgct ttgaaaaata
     2221 gcaacaacca tgatgtgctt agacaagaag accaggaaaa tttttcccaa ataagtacca
     2281 gcatccagaa aaaggccttt aaaacagctg tacccactga acagaagtgt gacattgaca
     2341 ttacctttga gtccgtgcct aatttgaatg actttaacca aagagggaat tctaatgctg
     2401 aatgtaatcc acattgtgct gaattataca ccaatcagat gtccactgaa actgaaatgg
     2461 ctatagggat tgaaaagaac tctttgtctt caaatgtacc gagtgaatct cagttacagc
     2521 ctgatcagcc tgatatacca ataacttcat ttgtttcact tggttgtgaa gctaacaatg
     2581 aaaacttaat tctctctggg aagagctctc aactgttatc ccaagatact tcattaactg
     2641 gcaagccatc taaaaaatct cagttctgtg aaaattcaaa tgatacagtg aaacttaaga
     2701 ttaattttca cggttccaag tcatcagatt ctagcgaagt tcacaagagt aaaatagaga
     2761 ttaatgtgtt agaaccagtt atgtcacagc tatcaaattg ccaagataat tcttgtcttc
     2821 aaagcgaaat actacctgtg tctgtggaaa gttcacattt aaatgtatct ggacaagtag
     2881 aaattcatct tggagactcc ctgtcttcga ctaaacagcc atctaatgat tcagcatctg
     2941 ttgagttaaa tcatacagaa aatgaagctc aggcatccaa gtctgagaat tcacaggagc
     3001 cttcatcttc tgtaaaagaa gagaatacga tttttctctc tttaggtgga aatgctaact
     3061 gtgagaaagt tgcactgacg cctccagaag gtactcctgt agaaaacagt cactcacttc
     3121 ctccagaatc cgtgtgttcg tcagtgggag attctcaccc tgagtcccaa aatactgatg
     3181 ataaaccttc tagcaacaac tcagcagaga tcgatgcatc aaatatcgtc tctctcaaag
     3241 ttatcattag tgatgatcca tttgtttcct cagatactga acttacaagt gctgtgtcta
     3301 gtattaatgg agaaaacctg ccaactataa tcttgtcatc tcctactaaa tcacctacca
     3361 aaaatgcaga actagttaaa tgcctatctt cagaagaaac tgtaggtgct gttgtatatg
     3421 ccgaagtagg ggattcagcc tcaatggaac agagtctttt aacattcaaa tctgaagact
     3481 cagcagtaaa caatactcag aatgaagatg gcattgcctt ttcagctaat gttacaccat
     3541 gtgtttccaa ggatggagga tatatacagc tgatgccagc cacaagcaca gcttttggca
     3601 attcaaataa cattctcata gctacctgtg tgactgatcc aacagcgtta ggaacatctg
     3661 taagtcagtc taatgtagtg gtgttgcctg gaaattctgc acctatgact gctcaacctc
     3721 taccaccaca gttacagaca ccaccaaggt caaacagtgt atttgcagtc aaccaagccg
     3781 tgtcaccaaa cttttcacaa ggatctgcca taataattgc ctctccagtc cagcctgtac
     3841 tccaaggaat ggtagggatg atcccagtat ctgtggttgg ccagaatgga aataactttt
     3901 ctactcctcc tcggcaggtt cttcatatgc ctttgacagc acctgtatgc aatagaagta
     3961 tccctcaatt ccccgtccct ccaaaatctc agaaggctca gggactaagg aacaagcctt
     4021 gtataggaaa acaagtaaat aatttggtgg attcgtcagg tcattcagtt ggatgtcatg
     4081 cacaaaaaac tgaagtttct gacaaatcta tcgccacaga tcttgggaaa aaatccgaag
     4141 aaaccacagt tcccttccca gaagagagta tagttccagc tgctaaaccc tgccacagac
     4201 gtgtactctg tttcgacagc actactgctc ctgtggcaaa tacgcagggg ccaaaccata
     4261 agatggtgtc gcaaaacaaa gagaggaatg cagtctcttt tcctaatctt gactcaccca
     4321 atgtgtcctc caccttaaaa cccccttcta ataatgctat caaaagagag aaagagaagc
     4381 ctcctctgcc taagatttta tctaaatcgg agagtgccat cagccggcat accaccataa
     4441 gagaaactca atcagaaaag aaagtttccc caacagaaat tgtgcttgaa tctttccata
     4501 aagcaacagc taataaggag aatgaattat gcagcgatgt agaaaggcag aaaaatccag
     4561 aaaattcaaa actatctatt ggacagcaaa atgggggttt gcgaagtgag aaatctatcg
     4621 cttcactgca agaaatgacc aaaaaacaag ggacatcttc aaacaataaa aatgtacttt
     4681 cagtaggcac cgctgtgaag gatctaaaac aagaacaaac taaatccgcc agttctttga
     4741 ttaccacaga aatgttacag gatatacaga ggcacagctc cgtgagtagg cttgctgata
     4801 gttccgattt acctgtgccc cggacacctg gctcaggggc cggggaaaaa cataaagaag
     4861 aacctataga tattatcaag gccccctcta gcaggcgttt cagtgaagac agtagcacat
     4921 caaaagtaat ggtgcctcct gtcacaccag acttgcctgc ctgcagccct gccagtgaaa
     4981 caggaagtga aaacagtgta aatatggctg cccacacatt aatgattctc tccagggcag
     5041 ccatttctag gactacttct gcaactcctc tgaaagataa cacacaacag tttagggcat
     5101 cttcaaggag caccaccaaa aagcggaaaa ttgaggaatt agatgaacgt gagcgaaact
     5161 ctcgtccttc tagtaaaaat cttacaaatt catccatacc aatgaaaaag aagaaaatta
     5221 agaaaaagaa gcttcccagt tcatttcccg caggaatgga tgtagacaaa tttttgttat
     5281 cattgcatta tgatgagttg gacccagctt tcttgtacaa agtggttgat tctagaggaa
     5341 agtcttaccc aactgtgagt gctgattacc aggacgccgt tgagaaggcg aagaagaagc
     5401 tcagaggctt catcgctgag aagagatgcg ctcctctaat gctccgtttg gcattccact
     5461 ctgctggaac ctttgacaag ggcacgaaga ccggtggacc cttcggaacc atcaagcacc
     5521 ctgccgaact ggctcacagc gctaacaacg gtcttgacat cgctgttagg cttttggagc
     5581 cactcaaggc ggagttccct attttgagct acgccgattt ctaccagttg gctggcgttg
     5641 ttgccgttga ggtcacgggt ggacctaagg ttccattcca ccctggaaga gaggacaagc
     5701 ctgagccacc accagagggt cgcttgcctg atcccactaa gggttctgac catttgagag
     5761 atgtgtttgg caaagctatg gggcttactg accaagatat cgttgctcta tctgggggtc
     5821 acactattgg agctgcacac aaggagcgtt ctggatttga gggtccctgg acctctaatc
     5881 ctcttatttt cgacaactca tacttcacgg agttgttgag tggtgagaag gaaggtctcc
     5941 ttcagctacc ttctgacaag gctcttttgt ctgaccctgt attccgccct ctcgttgata
     6001 aatatgcagc ggacgaagat gccttctttg ctgattacgc tgaggctcac caaaagcttt
     6061 ccgagcttgg gtttgctgat gccgactaca aagaccatga cggtgattat aaagatcatg
     6121 acatcgatta caaggatgac gatgacaagt aactcgagtc tagagggccc gtttaaaccc
     6181 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6241 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6301 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6361 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6421 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6481 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6541 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6601 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6661 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
     6721 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
     6781 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
     6841 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
     6901 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
     6961 cggccgcgct cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct
     7021 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
     7081 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
     7141 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
     7201 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
     7261 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
     7321 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
     7381 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
     7441 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
     7501 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
     7561 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
     7621 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
     7681 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
     7741 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
     7801 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7861 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7921 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7981 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8041 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8101 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8161 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8221 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8281 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8341 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8401 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8881 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9541 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9841 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10081 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10141 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10201 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
