LOCUS       sequence-367973-       16369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-Ki67-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16369)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 16369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: EGFP-Ki67-mCherry
FEATURES             Location/Qualifiers
     source          1..16369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        308..511
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     462..482
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(589..591,592..1302)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(631..652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(892..911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             4898..4909
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5264..5275
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5990..6001
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6722..6733
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8174..8185
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             11128..11838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11273..11291)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11541..11560
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          11908..12137
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     12120..12139
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(12208..12782,12783..12785,12786..12794)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12375..12392)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12602..12621
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12814..13413
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12814..12833)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13310..13330
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(13549..13566)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13555..13779
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13826..13845
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13905..13921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13905..13921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13918..13940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13929..13945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13953..13959,13960..13977,13978..13983))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13998..14019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14136..14153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14307..14895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14387..14406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15066..15857,15858..15926))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15689..15708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15927..16031)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(16106..16125)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(16297..16369,1..307)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
       61 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      121 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      181 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      241 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      301 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      361 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      421 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      481 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
      541 ctaccggact cagatctcga gctcaagctt cgaaccgcca tggctagcgt gagcaagggc
      601 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      661 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      721 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      781 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      841 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      901 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      961 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1021 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1081 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1141 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1201 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtccttaa ggagttcgtg
     1261 accgccgccg ggatcactct cggcatggac gagctgtaca agggcagcgg ccccagcggc
     1321 agcagcagct ggcccacgag acgcctggtt actatcaaaa ggagcggggt cgacggtccc
     1381 cactttcccc tgagcctcag cacctgcttg tttggaaggg gtattgaatg tgacatccgt
     1441 atccagcttc ctgttgtgtc aaaacaacat tgcaaaattg aaatccatga gcaggaggca
     1501 atattacata atttcagttc cacaaatcca acacaagtaa atgggtctgt tattgatgag
     1561 cctgtacggc taaaacatgg agatgtaata actattattg atcgttcctt caggtatgaa
     1621 aatgaaagtc ttcagaatgg aaggaagtca actgaatttc caagaaaaat acgtgaacag
     1681 gagccagcac gtcgtgtctc aagatctagc ttctcttctg accctgatga gaaagctcaa
     1741 gattccaagg cctattcaaa aatcactgaa ggaaaagttt caggaaatcc tcaggtacat
     1801 atcaagaatg tcaaagaaga cagtaccgca gatgactcaa aagacagtgt tgctcaggga
     1861 acaactaatg ttcattcctc agaacatgct ggacgtaatg gcagaaatgc agctgatccc
     1921 atttctgggg attttaaaga aatttccagc gttaaattag tgagccgtta tggagaattg
     1981 aagtctgttc ccactacaca atgtcttgac aatagcaaaa aaaatgaatc tcccttttgg
     2041 aagctttatg agtcagtgaa gaaagagttg gatgtaaaat cacaaaaaga aaatgtccta
     2101 cagtattgta gaaaatctgg attacaaact gattacgcaa cagagaaaga aagtgctgat
     2161 ggtttacagg gggagaccca actgttggtc tcgcgtaagt caagaccaaa atctggtggg
     2221 agcggccacg ctgtggcaga gcctgcttca cctgaacaag agcttgacca gaacaagggg
     2281 aagggaagag acgtggagtc tgttcagact cccagcaagg ctgtgggcgc cagctttcct
     2341 ctctatgagc cggctaaaat gaagacccct gtacaatatt cacagcaaca aaattctcca
     2401 caaaaacata agaacaaaga cctgtatact actggtagaa gagaatctgt gaatctgggt
     2461 aaaagtgaag gcttcaaggc tggtgataaa actcttactc ccaggaagct ttcaactaga
     2521 aatcgaacac cagctaaagt tgaagatgca gctgactctg ccactaagcc agaaaatctc
     2581 tcttccaaaa ccagaggaag tattcctaca gatgtggaag ttctgcctac ggaaactgaa
     2641 attcacaatg agccattttt aactctgtgg ctcactcaag ttgagaggaa gatccaaaag
     2701 gattccctca gcaagcctga gaaattgggc actacagctg gacagatgtg ctctgggtta
     2761 cctggtctta gttcagttga tatcaacaac tttggtgatt ccattaatga gagtgaggga
     2821 atacctttga aaagaaggcg tgtgtccttt ggtgggcacc taagacctga actatttgat
     2881 gaaaacttgc ctcctaatac gcctctcaaa aggggagaag ccccaaccaa aagaaagtct
     2941 ctggtaatgc acactccacc tgtcctgaag aaaatcatca aggaacagcc tcaaccatca
     3001 ggaaaacaag agtcaggttc agaaatccat gtggaagtga aggcacaaag cttggttata
     3061 agccctccag ctcctagtcc taggaaaact ccagttgcca gtgatcaacg ccgtaggtcc
     3121 tgcaaaacag cccctgcttc cagcagcaaa tctcagacag aggttcctaa gagaggaggg
     3181 agaaagagtg gcaacctgcc ttcaaagaga gtgtctatca gccgaagtca acatgatatt
     3241 ttacagatga tatgttccaa aagaagaagt ggtgcttcgg aagcaaatct gattgttgca
     3301 aaatcatggg cagatgtagt aaaacttggt gcaaaacaaa cacaaactaa agtcataaaa
     3361 catggtcctc aaaggtcaat gaacaaaagg caaagaagac ctgctactcc aaagaagcct
     3421 gtgggcgaag ttcacagtca atttagtaca ggccacgcaa actctccttg taccataata
     3481 atagggaaag ctcatactga aaaagtacat gtgcctgctc gaccctacag agtgctcaac
     3541 aacttcattt ccaaccaaaa aatggacttt aaggaagatc tttcaggaat agctgaaatg
     3601 ttcaagaccc cagtgaagga gcaaccgcag ttgacaagca catgtcacat cgctatttca
     3661 aattcagaga atttgcttgg aaaacagttt caaggaactg attcaggaga agaacctctg
     3721 ctccccacct cagagagttt tggaggaaat gtgttcttca gtgcacagaa tgcagcaaaa
     3781 cagccatctg ataaatgctc tgcaagccct cccttaagac ggcagtgtat tagagaaaat
     3841 ggaaacgtag caaaaacgcc caggaacacc tacaaaatga cttctctgga gacaaaaact
     3901 tcagatactg agacagagcc ttcaaaaaca gtatccactg caaacaggtc aggaaggtct
     3961 acagagttca ggaatataca gaagctacct gtggaaagta agagtgaaga aacaaataca
     4021 gaaattgttg agtgcatcct aaaaagaggt cagaaggcaa cactactaca acaaaggaga
     4081 gaaggagaga tgaaggaaat agaaagacct tttgagacat ataaggaaaa tattgaatta
     4141 aaagaaaacg atgaaaagat gaaagcaatg aagagatcaa gaacttgggg gcagaaatgt
     4201 gcaccaatgt ctgacctgac agacctcaag agcttgcctg atacagaact catgaaagac
     4261 acggcacgtg gccagaatct cctccaaacc caagatcatg ccaaggcacc aaagagtgag
     4321 aaaggcaaaa tcactaaaat gccctgccag tcattacaac cagaaccaat aaacacccca
     4381 acacacacaa aacaacagtt gaaggcatcc ctggggaaag taggtgtgaa agaagagctc
     4441 ctagcagtcg gcaagttcac acggacgtca ggggagacca cgcacacgca cagagagcca
     4501 gcaggagatg gcaagagcat cagaacgttt aaggagtctc caaagcagat cctggaccca
     4561 gcagcccgtg taactggaat gaagaagtgg ccaagaacgc ctaaggaaga ggcccagtca
     4621 ctagaagacc tggctggctt caaagagctc ttccagacac caggtccctc tgaggaatca
     4681 atgactgatg agaaaactac caaaatagcc tgcaaatctc caccaccaga atcagtggac
     4741 actccaacaa gcacaaagca atggcctaag agaagtctca ggaaagcaga tgtagaggaa
     4801 gaattcttag cactcaggaa actaacacca tcagcaggga aagccatgct tacgcccaaa
     4861 ccagcaggag gtgatgagaa agacattaaa gcatttatgg gaactccagt gcagaaactg
     4921 gacctggcag gaactttacc tggcagcaaa agacagctac agactcctaa ggaaaaggcc
     4981 caggctctag aagacctggc tggctttaaa gagctcttcc agactcctgg tcacaccgag
     5041 gaattagtgg ctgctggtaa aaccactaaa ataccctgcg actctccaca gtcagaccca
     5101 gtggacaccc caacaagcac aaagcaacga cccaagagaa gtatcaggaa agcagatgta
     5161 gagggagaac tcttagcgtg caggaatcta atgccatcag caggcaaagc catgcacacg
     5221 cctaaaccat cagtaggtga agagaaagac atcatcatat ttgtgggaac tccagtgcag
     5281 aaactggacc tgacagagaa cttaaccggc agcaagagac ggccacaaac tcctaaggaa
     5341 gaggcccagg ctctggaaga cctgactggc tttaaagagc tcttccagac ccctggtcat
     5401 actgaagaag cagtggctgc tggcaaaact actaaaatgc cctgcgaatc ttctccacca
     5461 gaatcagcag acaccccaac aagcacaaga aggcagccca agacaccttt ggagaaaagg
     5521 gacgtacaga aggagctctc agccctgaag aagctcacac agacatcagg ggaaaccaca
     5581 cacacagata aagtaccagg aggtgaggat aaaagcatca acgcgtttag ggaaactgca
     5641 aaacagaaac tggacccagc agcaagtgta actggtagca agaggcaccc aaaaactaag
     5701 gaaaaggccc aacccctaga agacctggct ggcttgaaag agctcttcca gacaccagta
     5761 tgcactgaca agcccacgac tcacgagaaa actaccaaaa tagcctgcag atcacaacca
     5821 gacccagtgg acacaccaac aagctccaag ccacagtcca agagaagtct caggaaagtg
     5881 gacgtagaag aagaattctt cgcactcagg aaacgaacac catcagcagg caaagccatg
     5941 cacacaccca aaccagcagt aagtggtgag aaaaacatct acgcatttat gggaactcca
     6001 gtgcagaaac tggacctgac agagaactta actggcagca agagacggct acaaactcct
     6061 aaggaaaagg cccaggctct agaagacctg gctggcttta aagagctctt ccagacacga
     6121 ggtcacactg aggaatcaat gactaacgat aaaactgcca aagtagcctg caaatcttca
     6181 caaccagacc cagacaaaaa cccagcaagc tccaagcgac ggctcaagac atccctgggg
     6241 aaagtgggcg tgaaagaaga gctcctagca gttggcaagc tcacacagac atcaggagag
     6301 actacacaca cacacacaga gccaacagga gatggtaaga gcatgaaagc atttatggag
     6361 tctccaaagc agatcttaga ctcagcagca agtctaactg gcagcaagag gcagctgaga
     6421 actcctaagg gaaagtctga agtccctgaa gacctggccg gcttcatcga gctcttccag
     6481 acaccaagtc acactaagga atcaatgact aatgaaaaaa ctaccaaagt atcctacaga
     6541 gcttcacagc cagacctagt ggacacccca acaagctcca agccacagcc caagagaagt
     6601 ctcaggaaag cagacactga agaagaattt ttagcattta ggaaacaaac gccatcagca
     6661 ggcaaagcca tgcacacacc caaaccagca gtaggtgaag agaaagacat caacacgttt
     6721 ttgggaactc cagtgcagaa actggaccag ccaggaaatt tacctggcag caatagacgg
     6781 ctacaaactc gtaaggaaaa ggcccaggct ctagaagaac tgactggctt cagagagctt
     6841 ttccagacac catgcactga taaccccacg actgatgaga aaactaccaa aaaaatactc
     6901 tgcaaatctc cgcaatcaga cccagcggac accccaacaa acacaaagca acggcccaag
     6961 agaagcctca agaaagcaga cgtagaggaa gaatttttag cattcaggaa actaacacca
     7021 tcagcaggca aagccatgca cacgcctaaa gcagcagtag gtgaagagaa agacatcaac
     7081 acatttgtgg ggactccagt ggagaaactg gacctgctag gaaatttacc tggcagcaag
     7141 agacggccac aaactcctaa agaaaaggcc aaggctctag aagatctggc tggcttcaaa
     7201 gagctcttcc agacaccagg tcacactgag gaatcaatga ccgatgacaa aatcacagaa
     7261 gtatcctgca aatctccaca accagaccca gtcaaaaccc caacaagctc caagcaacga
     7321 ctcaagatat ccttggggaa agtaggtgtg aaagaagagg tcctaccagt cggcaagctc
     7381 acacagacgt cagggaagac cacacagaca cacagagaga cagcaggaga tggaaagagc
     7441 atcaaagcgt ttaaggaatc tgcaaagcag atgctggacc cagcaaacta tggaactggg
     7501 atggagaggt ggccaagaac acctaaggaa gaggcccaat cactagaaga cctggccggc
     7561 ttcaaagagc tcttccagac accagaccac actgaggaat caacaactga tgacaaaact
     7621 accaaaatag cctgcaaatc tccaccacca gaatcaatgg acactccaac aagcacaagg
     7681 aggcggccca aaacaccttt ggggaaaagg gatatagtgg aagagctctc agccctgaag
     7741 cagctcacac agaccacaca cacagacaaa gtaccaggag atgaggataa aggcatcaac
     7801 gtgttcaggg aaactgcaaa acagaaactg gacccagcag caagtgtaac tggtagcaag
     7861 aggcagccaa gaactcctaa gggaaaagcc caacccctag aagacttggc tggcttgaaa
     7921 gagctcttcc agacaccaat atgcactgac aagcccacga ctcatgagaa aactaccaaa
     7981 atagcctgca gatctccaca accagaccca gtgggtaccc caacaatctt caagccacag
     8041 tccaagagaa gtctcaggaa agcagacgta gaggaagaat ccttagcact caggaaacga
     8101 acaccatcag tagggaaagc tatggacaca cccaaaccag caggaggtga tgagaaagac
     8161 atgaaagcat ttatgggaac tccagtgcag aaattggacc tgccaggaaa tttacctggc
     8221 agcaaaagat ggccacaaac tcctaaggaa aaggcccagg ctctagaaga cctggctggc
     8281 ttcaaagagc tcttccagac accaggcact gacaagccca cgactgatga gaaaactacc
     8341 aaaatagcct gcaaatctcc acaaccagac ccagtggaca ccccagcaag cacaaagcaa
     8401 cggcccaaga gaaacctcag gaaagcagac gtagaggaag aatttttagc actcaggaaa
     8461 cgaacaccat cagcaggcaa agccatggac acaccaaaac cagcagtaag tgatgagaaa
     8521 aatatcaaca catttgtgga aactccagtg cagaaactgg acctgctagg aaatttacct
     8581 ggcagcaaga gacagccaca gactcctaag gaaaaggctg aggctctaga ggacctggtt
     8641 ggcttcaaag aactcttcca gacaccaggt cacactgagg aatcaatgac tgatgacaaa
     8701 atcacagaag tatcctgtaa atctccacag ccagagtcat tcaaaacctc aagaagctcc
     8761 aagcaaaggc tcaagatacc cctggtgaaa gtggacatga aagaagagcc cctagcagtc
     8821 agcaagctca cacggacatc aggggagact acgcaaacac acacagagcc aacaggagat
     8881 agtaagagca tcaaagcgtt taaggagtct ccaaagcaga tcctggaccc agcagcaagt
     8941 gtaactggta gcaggaggca gctgagaact cgtaaggaaa aggcccgtgc tctagaagac
     9001 ctggttgact tcaaagagct cttctcagca ccaggtcaca ctgaagagtc aatgactatt
     9061 gacaaaaaca caaaaattcc ctgcaaatct cccccaccag aactaacaga cactgccacg
     9121 agcacaaaga gatgccccaa gacacgtccc aggaaagaag taaaagagga gctctcagca
     9181 gttgagaggc tcacgcaaac atcagggcaa agcacacaca cacacaaaga accagcaagc
     9241 ggtgatgagg gcatcaaagt attgaagcaa cgtgcaaaga agaaaccaaa cccagtagaa
     9301 gaggaaccca gcaggagaag gccaagagca cctaaggaaa aggcccaacc cctggaagac
     9361 ctggccggct tcacagagct ctctgaaaca tcaggtcaca ctcaggaatc actgactgct
     9421 ggcaaagcca ctaaaatacc ctgcgaatct cccccactag aagtggtaga caccacagca
     9481 agcacaaaga ggcatctcag gacacgtgtg cagaaggtac aagtaaaaga agagccttca
     9541 gcagtcaagt tcacacaaac atcaggggaa accacggatg cagacaaaga accagcaggt
     9601 gaagataaag gcatcaaagc attgaaggaa tctgcaaaac agacaccggc tccagcagca
     9661 agtgtaactg gcagcaggag acggccaaga gcacccaggg aaagtgccca agccatagaa
     9721 gacctagctg gcttcaaaga cccagcagca ggtcacactg aagaatcaat gactgatgac
     9781 aaaaccacta aaataccctg caaatcatca ccagaactag aagacaccgc aacaagctca
     9841 aagagacggc ccaggacacg tgcccagaaa gtagaagtga aggaggagct gttagcagtt
     9901 ggcaagctca cacaaacctc aggggagacc acgcacaccg acaaagagcc ggtaggtgag
     9961 ggcaaaggca cgaaagcatt taagcaacct gcaaagcgga agctggacgc agaagatgta
    10021 attggcagca ggagacagcc aagagcacct aaggaaaagg cccaacccct ggaagatctg
    10081 gccagcttcc aagagctctc tcaaacacca ggccacactg aggaactggc aaatggtgct
    10141 gctgatagct ttacaagcgc tccaaagcaa acacctgaca gtggaaaacc tctaaaaata
    10201 tccagaagag ttcttcgggc ccctaaagta gaacccgtgg gagacgtggt aagcaccaga
    10261 gaccctgtaa aatcacaaag caaaagcaac acttccctgc ccccactgcc cttcaagagg
    10321 ggaggtggca aagatggaag cgtcacggga accaagaggt tgcgctgcat gccagcacca
    10381 gaggaaattg tggaggagct gccagccagc aagaagcaga gggttgctcc cagggcaaga
    10441 ggcaaatcat ccgaacccgt ggtcatcatg aagagaagtt tgaggacttc tgcaaaaaga
    10501 attgaacctg cggaagagct gaacagcaac gacatgaaaa ccaacaaaga ggaacacaaa
    10561 ttacaagact cggtccctga aaataaggga atatccctgc gctccagacg ccaaaataag
    10621 actgaggcag aacagcaaat aactgaggtc tttgtattag cagaaagaat agaaataaac
    10681 agaaatgaaa agaagcccat gaagacctcc ccagagatgg acattcagaa tccagatgat
    10741 ggagcccgga aacccatacc tagagacaaa gtcactgaga acaaaaggtg cttgaggtct
    10801 gctagacaga atgagagctc ccagcctaag gtggcagagg agagcggagg gcagaagagt
    10861 gcgaaggttc tcatgcagaa tcagaaaggg aaaggagaag caggaaattc agactccatg
    10921 tgcctgagat caagaaagac aaaaagccag cctgcagcaa gcactttgga gagcaaatct
    10981 gtgcagagag taacgcggag tgtcaagagg tgtgcagaaa atccaaagaa ggctgaggac
    11041 aatgtgtgtg tcaagaaaat aagaaccaga agtcataggg acagtgaaga tatttgggat
    11101 ccatcgtcga gcggtccctc gggttcgatg gtgagcaagg gcgaggagga taacatggcc
    11161 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
    11221 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
    11281 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
    11341 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
    11401 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
    11461 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
    11521 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
    11581 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
    11641 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
    11701 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
    11761 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
    11821 gacgagctgt acaagtaaag cggccgcata gataactgat ccagtgtgct ggaattaatt
    11881 cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt
    11941 ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg
    12001 tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa
    12061 gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt
    12121 tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag
    12181 ggcggccaat tccgcccctc tccccccccc ctctccctcc ccccccccta acgttactgg
    12241 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
    12301 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
    12361 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
    12421 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
    12481 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
    12541 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
    12601 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
    12661 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
    12721 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
    12781 agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc
    12841 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac
    12901 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg
    12961 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
    13021 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
    13081 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
    13141 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
    13201 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
    13261 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
    13321 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
    13381 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
    13441 accgaaagga gcgcacgacc ccatggctcc gaccgaagcc acccggggcg gccccgccga
    13501 ccccgcaccc gcccccgagg cccaccgact ctagagctcg ctgatcagcc tcgactgtgc
    13561 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
    13621 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
    13681 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
    13741 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
    13801 gctggggctc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    13861 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    13921 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    13981 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    14041 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    14101 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    14161 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    14221 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    14281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    14341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    14401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    14461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    14521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    14581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    14641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    14701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    14761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    14821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    14881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    14941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    15001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    15061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    15121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    15181 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    15241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    15301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    15361 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    15421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    15481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    15541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    15601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    15661 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    15721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    15781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    15841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    15901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    15961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    16021 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    16081 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    16141 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    16201 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    16261 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
    16321 aatagtaatc aattacgggg tcattagttc atagcccata tatggagtt
//
