LOCUS       sequence-367978-        9683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ki67 (8 repeats+LR)-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 9683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
FEATURES             Location/Qualifiers
     source          1..9683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     461..481
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1511..1522
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4441..5151
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4696..4705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5220..5449
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5432..5451
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5521..6095,6096..6098,6099..6107)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5688..5705)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5915..5934
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6127..6726
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6127..6146)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6623..6643
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6862..6879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6868..7092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7139..7158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7218..7234)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7218..7234)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7231..7253)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7242..7258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7266..7272,7273..7290,7291..7296))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7311..7332
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7449..7466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7620..8208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7700..7719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8379..9170,9171..9239))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9002..9021
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9240..9344)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9419..9438)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9610..9683,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaatgaccg atgacaaaat cacagaagta
      601 tcctgcaaat ctccacaacc agacccagtc aaaaccccaa caagctccaa gcaacgactc
      661 aagatatcct tggggaaagt aggtgtgaaa gaagaggtcc taccagtcgg caagctcaca
      721 cagacgtcag ggaagaccac acagacacac agagagacag caggagatgg aaagagcatc
      781 aaagcgttta aggaatctgc aaagcagatg ctggacccag caaactatgg aactgggatg
      841 gagaggtggc caagaacacc taaggaagag gcccaatcac tagaagacct ggccggcttc
      901 aaagagctct tccagacacc agaccacact gaggaatcaa caactgatga caaaactacc
      961 aaaatagcct gcaaatctcc accaccagaa tcaatggaca ctccaacaag cacaaggagg
     1021 cggcccaaaa cacctttggg gaaaagggat atagtggaag agctctcagc cctgaagcag
     1081 ctcacacaga ccacacacac agacaaagta ccaggagatg aggataaagg catcaacgtg
     1141 ttcagggaaa ctgcaaaaca gaaactggac ccagcagcaa gtgtaactgg tagcaagagg
     1201 cagccaagaa ctcctaaggg aaaagcccaa cccctagaag acttggctgg cttgaaagag
     1261 ctcttccaga caccaatatg cactgacaag cccacgactc atgagaaaac taccaaaata
     1321 gcctgcagat ctccacaacc agacccagtg ggtaccccaa caatcttcaa gccacagtcc
     1381 aagagaagtc tcaggaaagc agacgtagag gaagaatcct tagcactcag gaaacgaaca
     1441 ccatcagtag ggaaagctat ggacacaccc aaaccagcag gaggtgatga gaaagacatg
     1501 aaagcattta tgggaactcc agtgcagaaa ttggacctgc caggaaattt acctggcagc
     1561 aaaagatggc cacaaactcc taaggaaaag gcccaggctc tagaagacct ggctggcttc
     1621 aaagagctct tccagacacc aggcactgac aagcccacga ctgatgagaa aactaccaaa
     1681 atagcctgca aatctccaca accagaccca gtggacaccc cagcaagcac aaagcaacgg
     1741 cccaagagaa acctcaggaa agcagacgta gaggaagaat ttttagcact caggaaacga
     1801 acaccatcag caggcaaagc catggacaca ccaaaaccag cagtaagtga tgagaaaaat
     1861 atcaacacat ttgtggaaac tccagtgcag aaactggacc tgctaggaaa tttacctggc
     1921 agcaagagac agccacagac tcctaaggaa aaggctgagg ctctagagga cctggttggc
     1981 ttcaaagaac tcttccagac accaggtcac actgaggaat caatgactga tgacaaaatc
     2041 acagaagtat cctgtaaatc tccacagcca gagtcattca aaacctcaag aagctccaag
     2101 caaaggctca agatacccct ggtgaaagtg gacatgaaag aagagcccct agcagtcagc
     2161 aagctcacac ggacatcagg ggagactacg caaacacaca cagagccaac aggagatagt
     2221 aagagcatca aagcgtttaa ggagtctcca aagcagatcc tggacccagc agcaagtgta
     2281 actggtagca ggaggcagct gagaactcgt aaggaaaagg cccgtgctct agaagacctg
     2341 gttgacttca aagagctctt ctcagcacca ggtcacactg aagagtcaat gactattgac
     2401 aaaaacacaa aaattccctg caaatctccc ccaccagaac taacagacac tgccacgagc
     2461 acaaagagat gccccaagac acgtcccagg aaagaagtaa aagaggagct ctcagcagtt
     2521 gagaggctca cgcaaacatc agggcaaagc acacacacac acaaagaacc agcaagcggt
     2581 gatgagggca tcaaagtatt gaagcaacgt gcaaagaaga aaccaaaccc agtagaagag
     2641 gaacccagca ggagaaggcc aagagcacct aaggaaaagg cccaacccct ggaagacctg
     2701 gccggcttca cagagctctc tgaaacatca ggtcacactc aggaatcact gactgctggc
     2761 aaagccacta aaataccctg cgaatctccc ccactagaag tggtagacac cacagcaagc
     2821 acaaagaggc atctcaggac acgtgtgcag aaggtacaag taaaagaaga gccttcagca
     2881 gtcaagttca cacaaacatc aggggaaacc acggatgcag acaaagaacc agcaggtgaa
     2941 gataaaggca tcaaagcatt gaaggaatct gcaaaacaga caccggctcc agcagcaagt
     3001 gtaactggca gcaggagacg gccaagagca cccagggaaa gtgcccaagc catagaagac
     3061 ctagctggct tcaaagaccc agcagcaggt cacactgaag aatcaatgac tgatgacaaa
     3121 accactaaaa taccctgcaa atcatcacca gaactagaag acaccgcaac aagctcaaag
     3181 agacggccca ggacacgtgc ccagaaagta gaagtgaagg aggagctgtt agcagttggc
     3241 aagctcacac aaacctcagg ggagaccacg cacaccgaca aagagccggt aggtgagggc
     3301 aaaggcacga aagcatttaa gcaacctgca aagcggaagc tggacgcaga agatgtaatt
     3361 ggcagcagga gacagccaag agcacctaag gaaaaggccc aacccctgga agatctggcc
     3421 agcttccaag agctctctca aacaccaggc cacactgagg aactggcaaa tggtgctgct
     3481 gatagcttta caagcgctcc aaagcaaaca cctgacagtg gaaaacctct aaaaatatcc
     3541 agaagagttc ttcgggcccc taaagtagaa cccgtgggag acgtggtaag caccagagac
     3601 cctgtaaaat cacaaagcaa aagcaacact tccctgcccc cactgccctt caagagggga
     3661 ggtggcaaag atggaagcgt cacgggaacc aagaggttgc gctgcatgcc agcaccagag
     3721 gaaattgtgg aggagctgcc agccagcaag aagcagaggg ttgctcccag ggcaagaggc
     3781 aaatcatccg aacccgtggt catcatgaag agaagtttga ggacttctgc aaaaagaatt
     3841 gaacctgcgg aagagctgaa cagcaacgac atgaaaacca acaaagagga acacaaatta
     3901 caagactcgg tccctgaaaa taagggaata tccctgcgct ccagacgcca aaataagact
     3961 gaggcagaac agcaaataac tgaggtcttt gtattagcag aaagaataga aataaacaga
     4021 aatgaaaaga agcccatgaa gacctcccca gagatggaca ttcagaatcc agatgatgga
     4081 gcccggaaac ccatacctag agacaaagtc actgagaaca aaaggtgctt gaggtctgct
     4141 agacagaatg agagctccca gcctaaggtg gcagaggaga gcggagggca gaagagtgcg
     4201 aaggttctca tgcagaatca gaaagggaaa ggagaagcag gaaattcaga ctccatgtgc
     4261 ctgagatcaa gaaagacaaa aagccagcct gcagcaagca ctttggagag caaatctgtg
     4321 cagagagtaa cgcggagtgt caagaggtgt gcagaaaatc caaagaaggc tgaggacaat
     4381 gtgtgtgtca agaaaataag aaccagaagt catagggaca gtgaagatat ttgggatcct
     4441 atggtgagca agggcgagga ggataacatg gcctctctcc cagcgacaca tgagttacac
     4501 atctttggct ccatcaacgg tgtggacttt gacatggtgg gtcagggcac cggcaatcca
     4561 aatgatggtt atgaggagtt aaacctgaag tccaccaagg gtgacctcca gttctccccc
     4621 tggattctgg tccctcatat cgggtatggc ttccatcagt acctgcccta ccctgacggg
     4681 atgtcgcctt tccaggccgc catggtagat ggctccggat accaagtcca tcgcacaatg
     4741 cagtttgaag atggtgcctc ccttactgtt aactaccgct acacctacga gggaagccac
     4801 atcaaaggag aggcccaggt gaaggggact ggtttccctg ctgacggtcc tgtgatgacc
     4861 aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga cttaccccaa cgacaaaacc
     4921 atcatcagta cctttaagtg gagttacacc actggaaatg gcaagcgcta ccggagcact
     4981 gcgcggacca cctacacctt tgccaagcca atggcggcta actatctgaa gaaccagccg
     5041 atgtacgtgt tccgtaagac ggagctcaag cactccaaga ccgagctcaa cttcaaggag
     5101 tggcaaaagg cctttaccga tgtgatgggc atggacgagc tgtacaagta agcggccgca
     5161 tagataactg atccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg
     5221 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     5281 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     5341 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     5401 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     5461 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccccccc
     5521 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     5581 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     5641 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     5701 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     5761 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     5821 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     5881 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     5941 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     6001 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     6061 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga
     6121 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
     6181 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
     6241 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6301 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6361 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6421 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6481 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6541 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6601 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6661 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6721 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatggc
     6781 tccgaccgaa gccacccggg gcggccccgc cgaccccgca cccgcccccg aggcccaccg
     6841 actctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6901 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6961 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7021 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7081 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgagtgca ttctagttgt
     7141 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7201 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7261 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7321 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7381 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7441 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7501 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7561 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8341 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8401 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8461 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8521 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     8581 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     8641 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     8701 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     8761 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     8821 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     8881 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     8941 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9001 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9061 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9361 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     9421 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     9481 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     9541 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     9601 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     9661 ttcatagccc atatatggag ttc
//
