LOCUS       sequence-367979-        9750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ki67 (shuffled Nterm)-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2
FEATURES             Location/Qualifiers
     source          1..9750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     461..481
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4508..5218
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4763..4772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5287..5516
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5499..5518
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5588..6162,6163..6165,6166..6174)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5755..5772)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5982..6001
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6194..6793
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6194..6213)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6690..6710
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(6929..6946)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6935..7159
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7206..7225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7285..7301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7285..7301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7298..7320)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7309..7325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7333..7339,7340..7357,7358..7363))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7378..7399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7516..7533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7687..8275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7767..7786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8446..9237,9238..9306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9069..9088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9307..9411)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9486..9505)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9677..9750,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc caccatggct tcctttggtg ggcacctaag
      601 acctgaacta tttgatgaaa acttgcctcc taatacgcct ctcaaaaggg gagaagcccc
      661 aaccaaaaga aagtctctgg taatgcacac tccacctgtc ctgaagaaaa tcatcaagga
      721 acagcctcaa ccatcaggaa aacaagagtc aggttcagaa atccatgtgg aagtgaaggc
      781 acaaagcttg gttataagcc ctccagctcc tagtcctagg aaaactccag ttgccagtga
      841 tcaacgccgt aggtcctgca aaacagcccc tgcttccagc agcaaatctc agacagaggt
      901 tcctaagaga ggagggagaa agagtggcaa cctgccttca aagagagtgt ctatcagccg
      961 aagtcaacat gatattttac agatgatatg ttccaaaaga agaagtggtg cttcggaagc
     1021 aaatctgatt gttgcaaaat catgggcaga tgtagtaaaa cttggtgcaa aacaaacaca
     1081 aactaaagtc ataaaacatg gtcctcaaag gtcaatgaac aaaaggcaaa gaagacctgc
     1141 tactccaaag aagcctgtgg gcgaagttca cagtcaattt agtacaggcc acgcaaactc
     1201 tccttgtacc ataataatag ggaaagctca tactgaaaaa gaaatccatg agcaggaggc
     1261 aatattacat aatttcagtt ccacaaatcc aacacaagta aatgggtctg ttattgatga
     1321 gcctgtacgg ctaaaacatg gagatgtaat aactattatt gatcgttcct tcaggtatga
     1381 aaatgaaagt cttcagaatg gaaggaagtc aactgaattt ccaagaaaaa tacgtgaaca
     1441 ggagccagca cgtcgtgtct caagatctag cttctcttct gaccctgatg agaaagctca
     1501 agattccaag gcctattcaa aaatcactga aggaaaagtt tcaggaaatc ctcaggtaca
     1561 tatcaagaat gtcaaagaag acagtaccgc agatgactca aaagacagtg ttgctcaggg
     1621 aacaactaat gttcattcct cagaacatgc tggacgtaat ggcagaaatg cagctgatcc
     1681 catttctggg gattttaaag aaatttccag cgttaaatta gtgagccgtt atggagaatt
     1741 gaagtctgtt cccactacac aatgtcttga caatagcaaa aaaaatgaat ctcccttttg
     1801 gaagctttat gagtcagtga agaaagagtt ggatgtaaaa tcacaaaaag aaaatgtcct
     1861 acagtattgt agaaaatctg gattacaaac tgattacgca acagagaaag aaagtgctga
     1921 tggtttacag ggggagaccc aactgttggt ctcgcgtaag tcaagaccaa aatctggtgg
     1981 gagcggccac gctgtggcag agcctgcttc acctgaacaa gagcttgacc agaacaaggg
     2041 gaagggaaga gacgtggagt ctgttcagac tcccagcaag gctgtgggcg ccagctttcc
     2101 tctctatgag ccggctaaaa tgaagacccc tgtacaatat tcacagcaac aaaattctcc
     2161 acaaaaacat aagaacaaag acctgtatac tactggtaga agagaatctg tgaatctggg
     2221 taaaagtgaa ggcttcaagg ctggtgataa aactcttact cccaggaagc tttcaactag
     2281 aaatcgaaca ccagctaaag ttgaagatgc agctgactct gccactaagc cagaaaatct
     2341 ctcttccaaa accagaggaa gtattcctac agatgtggaa gttctgccta cggaaactga
     2401 aattcacaat gagccatttt taactctgtg gctcactcaa gttgagagga agatccaaaa
     2461 ggattccctc agcaagcctg agaaattggg cactacagct ggacagatgt gctctgtgtt
     2521 acctggtctt agttcagttg atatcaacaa ctttggtgat tccattaatg agagtgaggg
     2581 aatacctttg aaaagaaggc gtgtggtaca tgtgcctgct cgaccctaca gagtgctcaa
     2641 caacttcatt tccaaccaaa aaatggactt taaggaagat ctttcaggaa tagctgaaat
     2701 gttcaagacc ccagtgaagg agcaaccgca gttgacaagc acatgtcaca tcgctatttc
     2761 aaattcagag aatttgcttg gaaaacagtt tcaaggaact gattcaggag aagaacctct
     2821 gctccccacc tcagagagtt ttggaggaaa tgtgttcttc agtgcacaga atgcagcaaa
     2881 acagccatct gataaatgct ctgcaagccc tcccttaaga cggcagtgta ttagagaaaa
     2941 tggaaacgta gcaaaaacgc ccaggaacac ctacaaaatg acttctctgg agacaaaaac
     3001 ttcagatact gagacagagc cttcaaaaac agtatccact gcaaacaggt caggaaggtc
     3061 tacagagttc aggaatatac agaagctacc tgtggaaagt aagagtgaag aaacaaatac
     3121 agaaattgtt gagtgcatcc taaaaagagg tcagaaggca acactactac aacaaaggag
     3181 agaaggagag atgaaggaaa tagaaagacc ttttgagaca tataaggaaa atattgaatt
     3241 aaaagaaaac gatgaaaaga tgaaagcaat gaagagatca agaacttggg ggcagaaatg
     3301 tgcaccaatg tctgacctga cagacctcaa gagcttgcct gatacagaac tcatgaaaga
     3361 cacggcacgt ggccagaatc tcctccaaac ccaagatcat gccaaggcat ggcccacgag
     3421 acgcctggtt actatcaaaa ggagcggggt cgacggtccc cactttcccc tgagcctcag
     3481 cacctgcttg tttggaaggg gtattgaatg tgacatccgt atccagcttc ctgttgtgtc
     3541 aaaacaacat tgcaaaatgt gcgctccaaa gcaaacacct gacagtggaa aacctctaaa
     3601 aatatccaga agagttcttc gggcccctaa agtagaaccc gtgggagacg tggtaagcac
     3661 cagagaccct gtaaaatcac aaagcaaaag caacacttcc ctgcccccac tgcccttcaa
     3721 gaggggaggt ggcaaagatg gaagcgtcac gggaaccaag aggttgcgct gcatgccagc
     3781 accagaggaa attgtggagg agctgccagc cagcaagaag cagagggttg ctcccagggc
     3841 aagaggcaaa tcatccgaac ccgtggtcat catgaagaga agtttgagga cttctgcaaa
     3901 aagaattgaa cctgcggaag agctgaacag caacgacatg aaaaccaaca aagaggaaca
     3961 caaattacaa gactcggtcc ctgaaaataa gggaatatcc ctgcgctcca gacgccaaaa
     4021 taagactgag gcagaacagc aaataactga ggtctttgta ttagcagaaa gaatagaaat
     4081 aaacagaaat gaaaagaagc ccatgaagac ctccccagag atggacattc agaatccaga
     4141 tgatggagcc cggaaaccca tacctagaga caaagtcact gagaacaaaa ggtgcttgag
     4201 gtctgctaga cagaatgaga gctcccagcc taaggtggca gaggagagcg gagggcagaa
     4261 gagtgcgaag gttctcatgc agaatcagaa agggaaagga gaagcaggaa attcagactc
     4321 catgtgcctg agatcaagaa agacaaaaag ccagcctgca gcaagcactt tggagagcaa
     4381 atctgtgcag agagtaacgc ggagtgtcaa gaggtgtgca gaaaatccaa agaaggctga
     4441 ggacaatgtg tgtgtcaaga aaataagaac cagaagtcat agggacagtg aagatatttg
     4501 ggatcctatg gtgagcaagg gcgaggagga taacatggcc tctctcccag cgacacatga
     4561 gttacacatc tttggctcca tcaacggtgt ggactttgac atggtgggtc agggcaccgg
     4621 caatccaaat gatggttatg aggagttaaa cctgaagtcc accaagggtg acctccagtt
     4681 ctccccctgg attctggtcc ctcatatcgg gtatggcttc catcagtacc tgccctaccc
     4741 tgacgggatg tcgcctttcc aggccgccat ggtagatggc tccggatacc aagtccatcg
     4801 cacaatgcag tttgaagatg gtgcctccct tactgttaac taccgctaca cctacgaggg
     4861 aagccacatc aaaggagagg cccaggtgaa ggggactggt ttccctgctg acggtcctgt
     4921 gatgaccaac tcgctgaccg ctgcggactg gtgcaggtcg aagaagactt accccaacga
     4981 caaaaccatc atcagtacct ttaagtggag ttacaccact ggaaatggca agcgctaccg
     5041 gagcactgcg cggaccacct acacctttgc caagccaatg gcggctaact atctgaagaa
     5101 ccagccgatg tacgtgttcc gtaagacgga gctcaagcac tccaagaccg agctcaactt
     5161 caaggagtgg caaaaggcct ttaccgatgt gatgggcatg gacgagctgt acaagtaagc
     5221 ggccgcatag ataactgatc cagtgtgctg gaattaattc gctgtctgcg agggccagct
     5281 gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt
     5341 ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg
     5401 cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg
     5461 agatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     5521 ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt ccgcccctct
     5581 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     5641 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5701 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5761 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5821 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5881 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5941 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6001 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6061 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     6121 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aacccacaag
     6181 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6241 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6301 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6361 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6421 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6481 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6541 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6601 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6661 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6721 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6781 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     6841 ccatggctcc gaccgaagcc acccggggcg gccccgccga ccccgcaccc gcccccgagg
     6901 cccaccgact ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6961 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7021 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7081 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     7141 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gagtgcattc
     7201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7261 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7321 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7381 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7441 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7501 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7561 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7621 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7681 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7741 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7801 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7861 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7921 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7981 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8041 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8101 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8161 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8221 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8281 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8341 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8401 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8461 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8521 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8581 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8641 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8701 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8761 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8821 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8881 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8941 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9001 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9061 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9121 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9181 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9241 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9301 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9361 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9421 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9481 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9541 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     9601 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     9661 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     9721 tcattagttc atagcccata tatggagttc
//
