LOCUS       sequence-367985-       15579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ki67-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15579)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 15579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: Ki67-mNeonGreen-IRESpuro2
FEATURES             Location/Qualifiers
     source          1..15579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     461..481
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4145..4156
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4511..4522
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5237..5248
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5969..5980
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7421..7432
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             10351..11061
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      10606..10615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          11130..11359
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     11342..11361
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    11417..11990
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11584..11601)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11811..11830
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             12023..12622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12023..12042)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12519..12539
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(12758..12775)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12764..12988
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13035..13054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13114..13130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13114..13130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13127..13149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13138..13154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13162..13168,13169..13186,13187..13192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13207..13228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13345..13362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13516..14104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13596..13615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14275..15066,15067..15135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14898..14917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15136..15240)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(15315..15334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(15506..15579,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaatgtggc ccacgagacg cctggttact
      601 atcaaaagga gcggggtcga cggtccccac tttcccctga gcctcagcac ctgcttgttt
      661 ggaaggggta ttgaatgtga catccgtatc cagcttcctg ttgtgtcaaa acaacattgc
      721 aaaattgaaa tccatgagca ggaggcaata ttacataatt tcagttccac aaatccaaca
      781 caagtaaatg ggtctgttat tgatgagcct gtacggctaa aacatggaga tgtaataact
      841 attattgatc gttccttcag gtatgaaaat gaaagtcttc agaatggaag gaagtcaact
      901 gaatttccaa gaaaaatacg tgaacaggag ccagcacgtc gtgtctcaag atctagcttc
      961 tcttctgacc ctgatgagaa agctcaagat tccaaggcct attcaaaaat cactgaagga
     1021 aaagtttcag gaaatcctca ggtacatatc aagaatgtca aagaagacag taccgcagat
     1081 gactcaaaag acagtgttgc tcagggaaca actaatgttc attcctcaga acatgctgga
     1141 cgtaatggca gaaatgcagc tgatcccatt tctggggatt ttaaagaaat ttccagcgtt
     1201 aaattagtga gccgttatgg agaattgaag tctgttccca ctacacaatg tcttgacaat
     1261 agcaaaaaaa atgaatctcc cttttggaag ctttatgagt cagtgaagaa agagttggat
     1321 gtaaaatcac aaaaagaaaa tgtcctacag tattgtagaa aatctggatt acaaactgat
     1381 tacgcaacag agaaagaaag tgctgatggt ttacaggggg agacccaact gttggtctcg
     1441 cgtaagtcaa gaccaaaatc tggtgggagc ggccacgctg tggcagagcc tgcttcacct
     1501 gaacaagagc ttgaccagaa caaggggaag ggaagagacg tggagtctgt tcagactccc
     1561 agcaaggctg tgggcgccag ctttcctctc tatgagccgg ctaaaatgaa gacccctgta
     1621 caatattcac agcaacaaaa ttctccacaa aaacataaga acaaagacct gtatactact
     1681 ggtagaagag aatctgtgaa tctgggtaaa agtgaaggct tcaaggctgg tgataaaact
     1741 cttactccca ggaagctttc aactagaaat cgaacaccag ctaaagttga agatgcagct
     1801 gactctgcca ctaagccaga aaatctctct tccaaaacca gaggaagtat tcctacagat
     1861 gtggaagttc tgcctacgga aactgaaatt cacaatgagc catttttaac tctgtggctc
     1921 actcaagttg agaggaagat ccaaaaggat tccctcagca agcctgagaa attgggcact
     1981 acagctggac agatgtgctc tgggttacct ggtcttagtt cagttgatat caacaacttt
     2041 ggtgattcca ttaatgagag tgagggaata cctttgaaaa gaaggcgtgt gtcctttggt
     2101 gggcacctaa gacctgaact atttgatgaa aacttgcctc ctaatacgcc tctcaaaagg
     2161 ggagaagccc caaccaaaag aaagtctctg gtaatgcaca ctccacctgt cctgaagaaa
     2221 atcatcaagg aacagcctca accatcagga aaacaagagt caggttcaga aatccatgtg
     2281 gaagtgaagg cacaaagctt ggttataagc cctccagctc ctagtcctag gaaaactcca
     2341 gttgccagtg atcaacgccg taggtcctgc aaaacagccc ctgcttccag cagcaaatct
     2401 cagacagagg ttcctaagag aggagggaga aagagtggca acctgccttc aaagagagtg
     2461 tctatcagcc gaagtcaaca tgatatttta cagatgatat gttccaaaag aagaagtggt
     2521 gcttcggaag caaatctgat tgttgcaaaa tcatgggcag atgtagtaaa acttggtgca
     2581 aaacaaacac aaactaaagt cataaaacat ggtcctcaaa ggtcaatgaa caaaaggcaa
     2641 agaagacctg ctactccaaa gaagcctgtg ggcgaagttc acagtcaatt tagtacaggc
     2701 cacgcaaact ctccttgtac cataataata gggaaagctc atactgaaaa agtacatgtg
     2761 cctgctcgac cctacagagt gctcaacaac ttcatttcca accaaaaaat ggactttaag
     2821 gaagatcttt caggaatagc tgaaatgttc aagaccccag tgaaggagca accgcagttg
     2881 acaagcacat gtcacatcgc tatttcaaat tcagagaatt tgcttggaaa acagtttcaa
     2941 ggaactgatt caggagaaga acctctgctc cccacctcag agagttttgg aggaaatgtg
     3001 ttcttcagtg cacagaatgc agcaaaacag ccatctgata aatgctctgc aagccctccc
     3061 ttaagacggc agtgtattag agaaaatgga aacgtagcaa aaacgcccag gaacacctac
     3121 aaaatgactt ctctggagac aaaaacttca gatactgaga cagagccttc aaaaacagta
     3181 tccactgcaa acaggtcagg aaggtctaca gagttcagga atatacagaa gctacctgtg
     3241 gaaagtaaga gtgaagaaac aaatacagaa attgttgagt gcatcctaaa aagaggtcag
     3301 aaggcaacac tactacaaca aaggagagaa ggagagatga aggaaataga aagacctttt
     3361 gagacatata aggaaaatat tgaattaaaa gaaaacgatg aaaagatgaa agcaatgaag
     3421 agatcaagaa cttgggggca gaaatgtgca ccaatgtctg acctgacaga cctcaagagc
     3481 ttgcctgata cagaactcat gaaagacacg gcacgtggcc agaatctcct ccaaacccaa
     3541 gatcatgcca aggcaccaaa gagtgagaaa ggcaaaatca ctaaaatgcc ctgccagtca
     3601 ttacaaccag aaccaataaa caccccaaca cacacaaaac aacagttgaa ggcatccctg
     3661 gggaaagtag gtgtgaaaga agagctccta gcagtcggca agttcacacg gacgtcaggg
     3721 gagaccacgc acacgcacag agagccagca ggagatggca agagcatcag aacgtttaag
     3781 gagtctccaa agcagatcct ggacccagca gcccgtgtaa ctggaatgaa gaagtggcca
     3841 agaacgccta aggaagaggc ccagtcacta gaagacctgg ctggcttcaa agagctcttc
     3901 cagacaccag gtccctctga ggaatcaatg actgatgaga aaactaccaa aatagcctgc
     3961 aaatctccac caccagaatc agtggacact ccaacaagca caaagcaatg gcctaagaga
     4021 agtctcagga aagcagatgt agaggaagaa ttcttagcac tcaggaaact aacaccatca
     4081 gcagggaaag ccatgcttac gcccaaacca gcaggaggtg atgagaaaga cattaaagca
     4141 tttatgggaa ctccagtgca gaaactggac ctggcaggaa ctttacctgg cagcaaaaga
     4201 cagctacaga ctcctaagga aaaggcccag gctctagaag acctggctgg ctttaaagag
     4261 ctcttccaga ctcctggtca caccgaggaa ttagtggctg ctggtaaaac cactaaaata
     4321 ccctgcgact ctccacagtc agacccagtg gacaccccaa caagcacaaa gcaacgaccc
     4381 aagagaagta tcaggaaagc agatgtagag ggagaactct tagcgtgcag gaatctaatg
     4441 ccatcagcag gcaaagccat gcacacgcct aaaccatcag taggtgaaga gaaagacatc
     4501 atcatatttg tgggaactcc agtgcagaaa ctggacctga cagagaactt aaccggcagc
     4561 aagagacggc cacaaactcc taaggaagag gcccaggctc tggaagacct gactggcttt
     4621 aaagagctct tccagacccc tggtcatact gaagaagcag tggctgctgg caaaactact
     4681 aaaatgccct gcgaatcttc tccaccagaa tcagcagaca ccccaacaag cacaagaagg
     4741 cagcccaaga cacctttgga gaaaagggac gtacagaagg agctctcagc cctgaagaag
     4801 ctcacacaga catcagggga aaccacacac acagataaag taccaggagg tgaggataaa
     4861 agcatcaacg cgtttaggga aactgcaaaa cagaaactgg acccagcagc aagtgtaact
     4921 ggtagcaaga ggcacccaaa aactaaggaa aaggcccaac ccctagaaga cctggctggc
     4981 ttgaaagagc tcttccagac accagtatgc actgacaagc ccacgactca cgagaaaact
     5041 accaaaatag cctgcagatc acaaccagac ccagtggaca caccaacaag ctccaagcca
     5101 cagtccaaga gaagtctcag gaaagtggac gtagaagaag aattcttcgc actcaggaaa
     5161 cgaacaccat cagcaggcaa agccatgcac acacccaaac cagcagtaag tggtgagaaa
     5221 aacatctacg catttatggg aactccagtg cagaaactgg acctgacaga gaacttaact
     5281 ggcagcaaga gacggctaca aactcctaag gaaaaggccc aggctctaga agacctggct
     5341 ggctttaaag agctcttcca gacacgaggt cacactgagg aatcaatgac taacgataaa
     5401 actgccaaag tagcctgcaa atcttcacaa ccagacccag acaaaaaccc agcaagctcc
     5461 aagcgacggc tcaagacatc cctggggaaa gtgggcgtga aagaagagct cctagcagtt
     5521 ggcaagctca cacagacatc aggagagact acacacacac acacagagcc aacaggagat
     5581 ggtaagagca tgaaagcatt tatggagtct ccaaagcaga tcttagactc agcagcaagt
     5641 ctaactggca gcaagaggca gctgagaact cctaagggaa agtctgaagt ccctgaagac
     5701 ctggccggct tcatcgagct cttccagaca ccaagtcaca ctaaggaatc aatgactaat
     5761 gaaaaaacta ccaaagtatc ctacagagct tcacagccag acctagtgga caccccaaca
     5821 agctccaagc cacagcccaa gagaagtctc aggaaagcag acactgaaga agaattttta
     5881 gcatttagga aacaaacgcc atcagcaggc aaagccatgc acacacccaa accagcagta
     5941 ggtgaagaga aagacatcaa cacgtttttg ggaactccag tgcagaaact ggaccagcca
     6001 ggaaatttac ctggcagcaa tagacggcta caaactcgta aggaaaaggc ccaggctcta
     6061 gaagaactga ctggcttcag agagcttttc cagacaccat gcactgataa ccccacgact
     6121 gatgagaaaa ctaccaaaaa aatactctgc aaatctccgc aatcagaccc agcggacacc
     6181 ccaacaaaca caaagcaacg gcccaagaga agcctcaaga aagcagacgt agaggaagaa
     6241 tttttagcat tcaggaaact aacaccatca gcaggcaaag ccatgcacac gcctaaagca
     6301 gcagtaggtg aagagaaaga catcaacaca tttgtgggga ctccagtgga gaaactggac
     6361 ctgctaggaa atttacctgg cagcaagaga cggccacaaa ctcctaaaga aaaggccaag
     6421 gctctagaag atctggctgg cttcaaagag ctcttccaga caccaggtca cactgaggaa
     6481 tcaatgaccg atgacaaaat cacagaagta tcctgcaaat ctccacaacc agacccagtc
     6541 aaaaccccaa caagctccaa gcaacgactc aagatatcct tggggaaagt aggtgtgaaa
     6601 gaagaggtcc taccagtcgg caagctcaca cagacgtcag ggaagaccac acagacacac
     6661 agagagacag caggagatgg aaagagcatc aaagcgttta aggaatctgc aaagcagatg
     6721 ctggacccag caaactatgg aactgggatg gagaggtggc caagaacacc taaggaagag
     6781 gcccaatcac tagaagacct ggccggcttc aaagagctct tccagacacc agaccacact
     6841 gaggaatcaa caactgatga caaaactacc aaaatagcct gcaaatctcc accaccagaa
     6901 tcaatggaca ctccaacaag cacaaggagg cggcccaaaa cacctttggg gaaaagggat
     6961 atagtggaag agctctcagc cctgaagcag ctcacacaga ccacacacac agacaaagta
     7021 ccaggagatg aggataaagg catcaacgtg ttcagggaaa ctgcaaaaca gaaactggac
     7081 ccagcagcaa gtgtaactgg tagcaagagg cagccaagaa ctcctaaggg aaaagcccaa
     7141 cccctagaag acttggctgg cttgaaagag ctcttccaga caccaatatg cactgacaag
     7201 cccacgactc atgagaaaac taccaaaata gcctgcagat ctccacaacc agacccagtg
     7261 ggtaccccaa caatcttcaa gccacagtcc aagagaagtc tcaggaaagc agacgtagag
     7321 gaagaatcct tagcactcag gaaacgaaca ccatcagtag ggaaagctat ggacacaccc
     7381 aaaccagcag gaggtgatga gaaagacatg aaagcattta tgggaactcc agtgcagaaa
     7441 ttggacctgc caggaaattt acctggcagc aaaagatggc cacaaactcc taaggaaaag
     7501 gcccaggctc tagaagacct ggctggcttc aaagagctct tccagacacc aggcactgac
     7561 aagcccacga ctgatgagaa aactaccaaa atagcctgca aatctccaca accagaccca
     7621 gtggacaccc cagcaagcac aaagcaacgg cccaagagaa acctcaggaa agcagacgta
     7681 gaggaagaat ttttagcact caggaaacga acaccatcag caggcaaagc catggacaca
     7741 ccaaaaccag cagtaagtga tgagaaaaat atcaacacat ttgtggaaac tccagtgcag
     7801 aaactggacc tgctaggaaa tttacctggc agcaagagac agccacagac tcctaaggaa
     7861 aaggctgagg ctctagagga cctggttggc ttcaaagaac tcttccagac accaggtcac
     7921 actgaggaat caatgactga tgacaaaatc acagaagtat cctgtaaatc tccacagcca
     7981 gagtcattca aaacctcaag aagctccaag caaaggctca agatacccct ggtgaaagtg
     8041 gacatgaaag aagagcccct agcagtcagc aagctcacac ggacatcagg ggagactacg
     8101 caaacacaca cagagccaac aggagatagt aagagcatca aagcgtttaa ggagtctcca
     8161 aagcagatcc tggacccagc agcaagtgta actggtagca ggaggcagct gagaactcgt
     8221 aaggaaaagg cccgtgctct agaagacctg gttgacttca aagagctctt ctcagcacca
     8281 ggtcacactg aagagtcaat gactattgac aaaaacacaa aaattccctg caaatctccc
     8341 ccaccagaac taacagacac tgccacgagc acaaagagat gccccaagac acgtcccagg
     8401 aaagaagtaa aagaggagct ctcagcagtt gagaggctca cgcaaacatc agggcaaagc
     8461 acacacacac acaaagaacc agcaagcggt gatgagggca tcaaagtatt gaagcaacgt
     8521 gcaaagaaga aaccaaaccc agtagaagag gaacccagca ggagaaggcc aagagcacct
     8581 aaggaaaagg cccaacccct ggaagacctg gccggcttca cagagctctc tgaaacatca
     8641 ggtcacactc aggaatcact gactgctggc aaagccacta aaataccctg cgaatctccc
     8701 ccactagaag tggtagacac cacagcaagc acaaagaggc atctcaggac acgtgtgcag
     8761 aaggtacaag taaaagaaga gccttcagca gtcaagttca cacaaacatc aggggaaacc
     8821 acggatgcag acaaagaacc agcaggtgaa gataaaggca tcaaagcatt gaaggaatct
     8881 gcaaaacaga caccggctcc agcagcaagt gtaactggca gcaggagacg gccaagagca
     8941 cccagggaaa gtgcccaagc catagaagac ctagctggct tcaaagaccc agcagcaggt
     9001 cacactgaag aatcaatgac tgatgacaaa accactaaaa taccctgcaa atcatcacca
     9061 gaactagaag acaccgcaac aagctcaaag agacggccca ggacacgtgc ccagaaagta
     9121 gaagtgaagg aggagctgtt agcagttggc aagctcacac aaacctcagg ggagaccacg
     9181 cacaccgaca aagagccggt aggtgagggc aaaggcacga aagcatttaa gcaacctgca
     9241 aagcggaagc tggacgcaga agatgtaatt ggcagcagga gacagccaag agcacctaag
     9301 gaaaaggccc aacccctgga agatctggcc agcttccaag agctctctca aacaccaggc
     9361 cacactgagg aactggcaaa tggtgctgct gatagcttta caagcgctcc aaagcaaaca
     9421 cctgacagtg gaaaacctct aaaaatatcc agaagagttc ttcgggcccc taaagtagaa
     9481 cccgtgggag acgtggtaag caccagagac cctgtaaaat cacaaagcaa aagcaacact
     9541 tccctgcccc cactgccctt caagagggga ggtggcaaag atggaagcgt cacgggaacc
     9601 aagaggttgc gctgcatgcc agcaccagag gaaattgtgg aggagctgcc agccagcaag
     9661 aagcagaggg ttgctcccag ggcaagaggc aaatcatccg aacccgtggt catcatgaag
     9721 agaagtttga ggacttctgc aaaaagaatt gaacctgcgg aagagctgaa cagcaacgac
     9781 atgaaaacca acaaagagga acacaaatta caagactcgg tccctgaaaa taagggaata
     9841 tccctgcgct ccagacgcca aaataagact gaggcagaac agcaaataac tgaggtcttt
     9901 gtattagcag aaagaataga aataaacaga aatgaaaaga agcccatgaa gacctcccca
     9961 gagatggaca ttcagaatcc agatgatgga gcccggaaac ccatacctag agacaaagtc
    10021 actgagaaca aaaggtgctt gaggtctgct agacagaatg agagctccca gcctaaggtg
    10081 gcagaggaga gcggagggca gaagagtgcg aaggttctca tgcagaatca gaaagggaaa
    10141 ggagaagcag gaaattcaga ctccatgtgc ctgagatcaa gaaagacaaa aagccagcct
    10201 gcagcaagca ctttggagag caaatctgtg cagagagtaa cgcggagtgt caagaggtgt
    10261 gcagaaaatc caaagaaggc tgaggacaat gtgtgtgtca agaaaataag aaccagaagt
    10321 catagggaca gtgaagatat ttgggatcct atggtgagca agggcgagga ggataacatg
    10381 gcctctctcc cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt
    10441 gacatggtgg gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag
    10501 tccaccaagg gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc
    10561 ttccatcagt acctgcccta ccctgacggg atgtcgcctt tccaggccgc catggtagat
    10621 ggctccggat accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt
    10681 aactaccgct acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact
    10741 ggtttccctg ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg
    10801 tcgaagaaga cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc
    10861 actggaaatg gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca
    10921 atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag
    10981 cactccaaga ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc
    11041 atggacgagc tgtacaagta agcggccgca tagataactg atccagtgtg ctggaattaa
    11101 ttcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctcaaaag cgggcatgac
    11161 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc
    11221 ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa aagacaatct ttttgttgtc
    11281 aagcttgagg tgtggcaggc ttgagatctg gccatacact tgagtgacaa tgacatccac
    11341 tttgcctttc tctccacagg tgtccactcc caggtccaac tgcaggtcga gcatgcatct
    11401 agggcggcca attccgcccc tctccccccc ccccccctaa cgttactggc cgaagccgct
    11461 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
    11521 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
    11581 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
    11641 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
    11701 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
    11761 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
    11821 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
    11881 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
    11941 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca
    12001 acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    12061 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    12121 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
    12181 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
    12241 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    12301 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    12361 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    12421 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    12481 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    12541 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    12601 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
    12661 cgcacgaccc catggctccg accgaagcca cccggggcgg ccccgccgac cccgcacccg
    12721 cccccgaggc ccaccgactc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
    12781 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
    12841 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
    12901 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
    12961 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
    13021 agtgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
    13081 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    13141 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    13201 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    13261 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    13321 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    13381 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    13441 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    13501 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    13561 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    13621 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    13681 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    13741 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    13801 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    13861 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    13921 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    13981 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    14041 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    14101 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    14161 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    14221 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    14281 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    14341 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    14401 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    14461 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    14521 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    14581 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    14641 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    14701 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    14761 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    14821 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    14881 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    14941 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    15001 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    15061 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    15121 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    15181 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    15241 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    15301 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    15361 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    15421 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
    15481 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
    15541 attacggggt cattagttca tagcccatat atggagttc
//
