LOCUS       sequence-368-exp        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Shen S, Arhin GK, Ullu E, Tschudi C
  TITLE     In vivo epitope tagging of Trypanosoma brucei genes using a one step
            PCR-based strategy.
  JOURNAL   Mol Biochem Parasitol 2001 Mar;113(1):171-3.
  PUBMED    11254965
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biochem
            Parasitol"; date: "2001-03"; volume: "113(1)"; pages: "171-3"
COMMENT     Sequence Label: BB2/neo
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             370..1164
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(424..443)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1034..1053
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gaggtccata ctaaccagga tccacttgac taaagcttaa agtgtgacaa cgtcgcacca
       61 tgtgtaggtt ttcatttatg ttctttcttt ctttcttttg tgaatttgtt ttctgtctca
      121 aatgttttta attcgcttgg gacctatgtt tttcttgttt ttttgctcac cctttgtgta
      181 ggaggcaccc tgtcacgtct gtggttgcgt gtatgccttc cttcccctta ttcgcttctt
      241 cctgtcgtgt cacacctctt tctccctctc cctttccgcc ttttctttca atcttgtttt
      301 ctcgaccagc cctactagag gagaaagaat agtaaccctt tcatcaaata gtccaaacga
      361 attgaattca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
      421 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
      481 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
      541 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
      601 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
      661 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
      721 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
      781 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
      841 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
      901 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
      961 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1021 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1081 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1141 cgccttcttg acgagttctt ctga
//
