LOCUS       sequence-368005-       11185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused 
            to HA and GFP with Y180L, Q182L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Brown JT, Haraczy AJ, Wilhelm CM, Belanger KD
  TITLE     Characterization of nuclear pore complex targeting domains in Pom152
            in Saccharomyces cerevisiae.
  JOURNAL   Biol Open. 2021 Oct 15;10(10). pii: 272274. doi: 10.1242/bio.057661.
            Epub 2021 Oct 20.
  PUBMED    34557894
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2021-10-15"; volume: "10(10). pii: 272274. doi"; 
            pages: " 10.1242/bio.057661. Epub 2021 Oct 20"
COMMENT     Sequence Label: KBB553
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        353..457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(458..526,527..1318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(676..695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1489..2077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1978..1997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2231..2248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2365..2386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2401..2406,2407..2424,2425..2431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2439..2455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2444..2466
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2463..2479
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2463..2479
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2500..2518
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2546..2562
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2547..2563
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4994..5704
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5021..5049)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5644..5664
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7776..7792)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7776..7792)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7824..7843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7825..7843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7850..7867)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7850..7866)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7859..7881)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8007..8462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8094..8113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8304..8325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8763..9170
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             9171..10265
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(10759..10778)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10878..10900
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10938..10956)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(10998..11185,1..316)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
       61 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
      121 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
      181 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
      241 tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat
      301 ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac
      361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      421 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1141 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1621 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2101 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2161 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2221 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2281 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2341 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2401 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2461 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
     2521 acaaaagctg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
     2581 ttcctgcagc ccgggggatc cacagttgac aacgatctca agattggttg ggatttaaat
     2641 cctatagcag tgcatcagaa gaagcaaaaa ggccagtgga cagtcattaa gtttatcacc
     2701 agaaagggct tccagctgag agatcccctc ataatttccc caaaacgtaa ccatgtgtga
     2761 atagattttg agtaagcagg gttgcagcca cgagtgagtc ttcccttgtt attgtgtagc
     2821 cagaatgccg caaaacttcc atgcctaagc gaactgttga gagtacgttt cgatttctga
     2881 ctgtgttagc ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc
     2941 aacatgttag ttgaagcatc agggcgatta gcagcatgat atcaaaacgc tctgagctgc
     3001 tcgttcggct atggcgtagg cctagtccgt aggcaggact tttcaagtct cggaaggttt
     3061 cttcaatctg cattcgcttc gaatagatat taacaagttg tttgggtgtt cgaatttcaa
     3121 caggtaagtt agttgctaga atccatggct cctttgccga cgctgagtag attttaggtg
     3181 acgggtggtg acaatgagtc cgtgtcgagc gctgattttt tcggccttta gagcgagatt
     3241 tatacaatag aatttggcat gagattgggt tgcttttagt cagcctctta tagcctaaag
     3301 tctttgagtg actagatgac atatcatgta agttgctgat aggtttccag ttttccgctc
     3361 ctaggtctgc atattgtact tttcctctta ctcgacttaa ccagtaccaa cccagcttct
     3421 caacggattt ataccatggc actttaaagc cagcatcact gacaatgagc ggtgtggtgt
     3481 tactcggtag aatgctcgca aggtcggcta gaaattggtc atgagctttc tttgaacatt
     3541 gctctgaaag cgggaacgct ttctcataaa gagtaacaga acgaccgtgt agtgcgactg
     3601 aagctcgcaa taccataagc cgtttttgct cacggatatc agaccagtca acaagtacaa
     3661 tgggcatcgt attgcccgaa cagataaagc tagcatgcca acggtataca gcgagtcgct
     3721 ctttgtggag gtgacgatta cctaacaatc ggtcgattcg tttgatgtta tgttttgttc
     3781 tcgctttggt tggcaggtta cggccaagtt cggtaagagt gagagtttta cagtcaagta
     3841 aggcgtggca agccaacgtt aagctgttga gtcgttttaa gtgtaattcg gggcagaatt
     3901 ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatttt gttgtctgat tattgatttt
     3961 tggcgaaacc atttgatcat atgacaagat gtgtatctac cttaacttaa tgattttgat
     4021 aaaaatcatt aggggattca tcagggcttc cagaccatca caggtgaaga gaaagaaaaa
     4081 aaataatgcg ctggtattat agaaatagag tttcattata taagaacata taggaagaat
     4141 acattattcc tatctagggc atcaaataat tgcaaccact taatgttaac ttccttcata
     4201 attcaaaaca ttatgttcta tagcactatg tattttatgt cattatatac gtagtcaata
     4261 actttatacg ttttttttat tgaacaaaag taagaattgc ttaagacata ataagaggaa
     4321 actcatcgaa ccaattcaaa acggattata gatttatcat accagatacg tttatcagga
     4381 ggcttgtata atggagcaca gatataacgt gtttaatgat actccacgag ggaaccattg
     4441 gatgggcagt tctgtgtcag gatcaccacg tccatcttat agcagtcgtc ctaatgtaaa
     4501 cacaacacga cgcttccaat atagtgacga tgagcccgct gagaaaatcc gacctttacg
     4561 ctcgaggagc tttaaaagta ctgaaagtaa cataagcgat gaaaaatcaa ggatatctga
     4621 acgtgacagc aaagaccgct acattaatgg tgataaaaag gtagacattt attcactgcc
     4681 tctaatatca accgatgttt tagaaatttc caaacaaagg acatttgcgg tgatattgtt
     4741 tttaataatt caatgttata aaatatatga cctggtaata cttaaatcgg ggttaccgct
     4801 ttcgggttta ctgtttaaga actatcgatt taatttcata tccaagtatt tcattataga
     4861 ttccttcttc ctgtatgttt taccatcctt taacattcct aggttgactt ttaagccatg
     4921 ggttgtttta ctcctgattt tagctatgct actattgaac attttcatat caagcgatca
     4981 cgagttcgtt gctagcaaag gagaagaact cttcactgga gttgtcccaa ttcttgttga
     5041 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     5101 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     5161 gccaacactt gtcactactt tcacttatgg tgttcagtgc ttttcaagat acccggatca
     5221 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     5281 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     5341 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     5401 tgggcacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     5461 aaagaatgga atcaaagcta acttcaaaat tagacacaac attgaagatg gaagcgttca
     5521 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     5581 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     5641 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     5701 caaaggatcc ggctgctaac aaagcccgaa aggacggtct atagaggtgt gttaaaagca
     5761 gacttttgaa catccacaga aattggctca atgacctatc cgaaaagaat acctataaat
     5821 gcgtggtctg aagttcatag ggtggcaagg ccactaatta ttactttcga cgcatataat
     5881 acactatacg ccacaaaact tcccgtcatg gaacaatact gcattgttgg gcgtaaatat
     5941 ggtatcaagg cgaatccttc gactttaaca aacaattttc cacacgtttt taaaaaactg
     6001 aaggaagact atccccaata cggaaaatat tctggtatca agcccgaaca atggtggtcc
     6061 atcttgatta ggaacgtctt cgcaccaaac gaaattcccg atgagatgat aaatgaaata
     6121 ttaatgcggt ttgagggctt cgattcgtat tttgtttatc cagatttgat caaattttta
     6181 aaggatttga agtcaagaca tcctgatgtt atcttaggaa tagtaagcaa tactgatcca
     6241 attttttaca aattattaaa gaatatcgga ttatttgaga cattttccgg tcacatttat
     6301 ttatcatatg agcttaatct cgccaaacct gatagagcaa tatttcaata tgcgcttgac
     6361 gatattatat ctaaacaacc tcatttactc gaaaagtaca ccagggagga aattctacag
     6421 cattgctttc acatcggaga tgaattaaag aacgatctcg aaggggcaga agctgccggt
     6481 tggactggta tactgttaga tagaaatgat aaatacgggt ttctttcaaa ctcgattagt
     6541 aaaccaatgc gggacgaata caagctgtca attgataaaa tagataataa ttcaataaac
     6601 acttgggaag ccaatactaa gcaaactgac acgttacagt tgagtgaaag aaagtacgtt
     6661 gtttcgaatc ttgaggtttt agaggaactc tttccctgag cgcctaacgt tacagtaaca
     6721 aatatgtatg atagatatta ttcatatccg ccagtaatat ttactatatc tttgaatggg
     6781 tttgtttatt aataatctac tatgtgtaaa tatatgatcc aagtattata cagtttagac
     6841 actgattgtt ttccagacgt tcaaaccgtc agtaccggta cttactaaac aacctgggat
     6901 ttgcttatgc catttgacat ctttaacttc cttttgccag tgaacaaaca ataattgtgg
     6961 tggaatttct tgtagctctt ttgtttcggc ggcctgttgt ttaatttcct catcgtcagc
     7021 ttctacagac aaatcccaca aagtcacagt attatcttct gagcctaccg caacgataga
     7081 ctcatctaat gggttgaatg caatggaagt aatggctccc ttatggaagt catattgagc
     7141 aaccggttgg acggcgtcag cattacttgg cgtaaactgt cttaaatccc atactcccca
     7201 tgtaccgtta tcgtcaccgc ttgctagcaa gtaaccaatt ttatcactcc aacttatgac
     7261 atttacgtca gtattagaag ctttaacaga gatagcaggt ttatgttttt ttgatcttgt
     7321 gtcccaaatc cttatatatc catcacatcc tgcggttgca aaaacggtgg attcagtgcg
     7381 agaccactgg atatcttcta tggatttatt gtttgaaaca gtaaatggtt gtttatcagt
     7441 cacccatctc gatgtgtgcc tttgggtaaa atatatttgt cctgagcaat cacctgatag
     7501 taacgcacca gtcttgatca atggtgacca atccaacccg tagccttcaa cattcccatg
     7561 attttttaca gtgtgaatag gacgcttagc agacttcgga atctgataac ctggtgtgga
     7621 aaaagctttg ctttgtggag ctagattgta tatataaaca tcaccatttt cgctcattgt
     7681 agcggttaac acctcttgat tagaaatggc aaaaggagaa acctttaatc tattggttgt
     7741 atctctcaat ggtatatttt cattctcaat gactggatcc actagttcta gagcggccgc
     7801 caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     7861 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7921 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7981 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     8041 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8101 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8161 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8221 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8281 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8341 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8401 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8461 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatcgacc
     8521 ggtcgaggag aacttctagt atatctacat acctaatatt attgccttat taaaaatgga
     8581 atcccaacaa ttacatcaaa atccacattc tcttcaaaat caattgtcct gtacttcctt
     8641 gttcatgtgt gttcaaaaac gttatattta taggataatt atactctatt tctcaacaag
     8701 taattggttg tttggccgag cggtctaagg cgcctgattc aagaaatatc ttgaccgcag
     8761 ttaactgtgg gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat
     8821 tatttttttc ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg
     8881 atgactggaa attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg
     8941 aaaaattggg agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga
     9001 gaagcgttca tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg
     9061 acctattgtt ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct
     9121 tttacatttc agcaatatat atatatatat ttcaaggata taccattcta atgtctgccc
     9181 ctaagaagat cgtcgttttg ccaggtgacc acgttggtca agaaatcaca gccgaagcca
     9241 ttaaggttct taaagctatt tctgatgttc gttccaatgt caagttcgat ttcgaaaatc
     9301 atttaattgg tggtgctgct atcgatgcta caggtgttcc acttccagat gaggcgctgg
     9361 aagcctccaa gaaggctgat gccgttttgt taggtgctgt gggtggtcct aaatggggta
     9421 ccggtagtgt tagacctgaa caaggtttac taaaaatccg taaagaactt caattgtacg
     9481 ccaacttaag accatgtaac tttgcatccg actctctttt agacttatct ccaatcaagc
     9541 cacaatttgc taaaggtact gacttcgttg ttgtcagaga attagtggga ggtatttact
     9601 ttggtaagag aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg
     9661 ttccagaagt gcaaagaatc acaagaatgg ccgctttcat ggccctacaa catgagccac
     9721 cattgcctat ttggtccttg gataaagcta atgttttggc ctcttcaaga ttatggagaa
     9781 aaactgtgga ggaaaccatc aagaacgaat tccctacatt gaaggttcaa catcaattga
     9841 ttgattctgc cgccatgatc ctagttaaga acccaaccca cctaaatggt attataatca
     9901 ccagcaacat gtttggtgat atcatctccg atgaagcctc cgttatccca ggttccttgg
     9961 gtttgttgcc atctgcgtcc ttggcctctt tgccagacaa gaacaccgca tttggtttgt
    10021 acgaaccatg ccacggttct gctccagatt tgccaaagaa taaggtcaac cctatcgcca
    10081 ctatcttgtc tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg
    10141 ccattgaaga tgcagttaaa aaggttttgg atgcaggtat cagaactggt gatttaggtg
    10201 gttccaacag taccaccgaa gtcggtgatg ctgtcgccga agaagttaag aaaatccttg
    10261 cttaaaaaga ttctcttttt ttatgatatt tgtacataaa ctttataaat gaaattcata
    10321 atagaaacga cacgaaatta caaaatggaa tatgttcata gggtagacga aactatatac
    10381 gcaatctaca tacatttatc aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag
    10441 caaattgata ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta acattaatat
    10501 tgacaaggag gagggcacca cacaaaaagt taggtgtaac agaaaatcat gaaactatga
    10561 ttcctaattt atatattgga ggattttctc taaaaaaaaa aaaatacaac aaataaaaaa
    10621 cactcaatga cctgaccatt tgatggagtt taagtcaata ccttcttgaa ccatttccca
    10681 taatggtgaa agttccctca agaattttac tctgtcagaa acggccttaa cgacgtagtc
    10741 gatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
    10801 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
    10861 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
    10921 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
    10981 aatggtttct taggacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg
    11041 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt
    11101 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg
    11161 tttaaaaaat ttcaacaaaa agcgt
//
