LOCUS       sequence-368060-        9600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NAD+ biosensor comprised of a circularly permuted Venus fluorescent 
            protein (cpVenus), a bipartite NAD+-binding domain modeled from 
            bacterial DNA ligase, and a localization tag totarget mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Cambronne XA, Stewart ML, Kim D, Jones-Brunette AM, Morgan RK, 
            Farrens DL, Cohen MS, Goodman RH
  TITLE     Biosensor reveals multiple sources for mitochondrial NAD(+).
  JOURNAL   Science. 2016 Jun 17;352(6292):1474-7. doi: 10.1126/science.aad5168.
  PUBMED    27313049
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-17- 17"; volume: "352"; 
            issue: "6292"; pages: "1474-7"
COMMENT     Sequence Label: mito NAD+ biosensor
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2919..2942
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2955..2981
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2997..3021
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3789..4040
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3977..3998
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4101..4122)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4362..4381)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(4756..4780)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4793..5360
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4810..5362
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4954..4971)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5181..5200
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5374..5973
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5374..5393)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5870..5890
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5990..6578
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6043..6063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6461..6472)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6653..6886
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7005..7023)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7091..7195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7196..7264,7265..8056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7414..7433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8227..8815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8716..8735
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8969..8986
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9226..9247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9262..9267,9268..9285,9286..9292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9300..9316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9305..9327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9324..9340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9324..9340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9390..9409
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct
     2821 ctcgaggttg atctgccacc atgctggcca cccgcgtgtt cagcctggtg ggcaagcgcg
     2881 ccatcagcac cagcgtgtgc gtgcgcgccc acaccggtga ctacaaggat gacgatgaca
     2941 agctcgatgg aggataccca tacgatgttc cagattacgc tgctcgaggt tatcaaacaa
     3001 gtttgtacaa aaaagcaggc tccaccatgg ggcatatgga aaaagctccg catgacattc
     3061 caatgtatag tttaaatgat gggtttagca aggaagacat ttttgctttt gatgaacgtg
     3121 ttcgtaaggc gattggtaaa cccgttgctt actgttgtga gttactaatt gatgggttag
     3181 caatttcatt acgttatgaa aacggggtgt ttgtacgcgg agcaacacga ggcgatggaa
     3241 cggttggtga aaacattaca gagaacttac gaacggttcg ctctgtaccg atgcgtttaa
     3301 cggagcccat ttcagtggaa gttcgtggcg agtgctacat gcctaaacaa tcctttgtcg
     3361 cattgaatga agaacgtgaa gaaaatggcc aagatatttt tgccaatcct cggaatgcag
     3421 cggcgggaag cttgcgccaa ctagatacaa aaattgtggc taaacggaac ttaaatacgt
     3481 ttttatacac agttgctgac tttggaccaa tgaaagcgaa gacgcagttt gaggcattag
     3541 aagagctttc tgcaattggt tttagaacca atcccgaacg gcaattatgt cagtcaattg
     3601 atgaagtttg ggcctacatt gaagaatacc atgaaaagcg gagcactttg ccatacgaaa
     3661 tcaatggaat tgtgattaaa gtgaacgaat ttgcgttgca agatgaatta ggtttcactg
     3721 tcaaagcacc tcgctgggcg attgcctaca aatttccata caacagcgac aacgtctaca
     3781 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     3841 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3901 ccgtgctgct gcccgacaac cactacctga gcttccagtc caagctgagc aaagacccca
     3961 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     4021 gcatggacga gctgtacaag ggcggttccg gaggcatggt gagcaagggc gaggagctgt
     4081 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4141 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     4201 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggtc
     4261 tccaatgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4321 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4381 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4441 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct tgagtacaac ggcaccatag
     4501 ttctcgaggg taccagatct gaacaacaac cattaacatt aacggcggcg acaactcgtg
     4561 cgcaagaatt aagaaaacaa ctcaatcaat attcccatga atattatgtc aaagatcaac
     4621 catccgtaga agattatgtt tacgatcgtt tgtataaaga gttagtcgat attgaaacgg
     4681 agtttcctga tttaatcacg ccagattcgc cgacacaacg tgtaggttaa atcgatgagc
     4741 tctcgagata tctagaccca gctttcttgt acaaagtggt tcgataacga attccgcccc
     4801 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4861 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4921 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4981 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5041 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5101 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     5161 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     5221 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     5281 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     5341 tttgaaaaac acgatgataa tatggccaca accatgaccg agtacaagcc cacggtgcgc
     5401 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     5461 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     5521 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     5581 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     5641 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     5701 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     5761 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     5821 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     5881 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     5941 acctggtgca tgacccgcaa gcccggtgcc tgatgcatcg atggatccta atcaacctct
     6001 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6061 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6121 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6181 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6241 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6301 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6361 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6421 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6481 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6541 tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg
     6601 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6661 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     6721 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6781 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6841 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     6901 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc
     6961 gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct
     7021 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7081 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7141 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7201 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7261 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7321 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7381 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     7441 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7501 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7561 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7621 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7681 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7741 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7801 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7861 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7921 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7981 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8041 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8101 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8161 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8221 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8281 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8341 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8401 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8461 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8521 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8581 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8641 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8701 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8761 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8821 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8881 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8941 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9001 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat
     9061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca
     9181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     9241 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     9301 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa
     9361 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9421 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     9481 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     9541 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
