LOCUS       sequence-368067-       11185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA-tagged POM152 with glycosylation sites mutated and with GFP at 
            C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Brown JT, Haraczy AJ, Wilhelm CM, Belanger KD
  TITLE     Characterization of nuclear pore complex targeting domains in Pom152
            in Saccharomyces cerevisiae.
  JOURNAL   Biol Open. 2021 Oct 15;10(10). pii: 272274. doi: 10.1242/bio.057661.
            Epub 2021 Oct 20.
  PUBMED    34557894
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2021-10-15"; volume: "10(10). pii: 272274. doi"; 
            pages: " 10.1242/bio.057661. Epub 2021 Oct 20"
COMMENT     Sequence Label: KBB464
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..64
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(283..387)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    424..927
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     969..987
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1025..1047)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1147..1166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1660..2754)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2755..3162)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3463..3918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3600..3621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3812..3831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4044..4066
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4058..4075
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4059..4075
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4082..4101
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4082..4100
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4133..4149
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4133..4149
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(6221..6931)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6261..6281)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     6876..6904
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(9362..9378)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9363..9379)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(9407..9425)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9446..9462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9446..9462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9459..9481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9470..9486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9494..9500,9501..9518,9519..9524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9539..9560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9677..9694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9848..10436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9928..9947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10607..11185,1..213,214..282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
       61 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
      121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
      181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
      241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
      301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
      361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcctt
      421 ttcatcacgt gctataaaaa taattataat ttaaattttt taatataaat atataaatta
      481 aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa
      541 aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg
      601 tattaatgcc gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga
      661 ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta
      721 ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt
      781 tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa
      841 cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag
      901 gcgcgtgtaa gttacaggca agcgatccgt cctaagaaac cattattatc atgacattaa
      961 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     1021 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     1081 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     1141 actatgcggc atcagagcag attgtactga gagtgcacca tatcgactac gtcgttaagg
     1201 ccgtttctga cagagtaaaa ttcttgaggg aactttcacc attatgggaa atggttcaag
     1261 aaggtattga cttaaactcc atcaaatggt caggtcattg agtgtttttt atttgttgta
     1321 tttttttttt tttagagaaa atcctccaat atataaatta ggaatcatag tttcatgatt
     1381 ttctgttaca cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt
     1441 gcaattcttt ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt
     1501 tacatcctcc tttttctcct tcttgataaa tgtatgtaga ttgcgtatat agtttcgtct
     1561 accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat
     1621 aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac
     1681 ttcttcggcg acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt
     1741 tctgatacct gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg
     1801 caagttcaat gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac
     1861 cttattcttt ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt
     1921 gttcttgtct ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat
     1981 aacggaggct tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt
     2041 taggtgggtt gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac
     2101 cttcaatgta gggaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga
     2161 agaggccaaa acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag
     2221 ggccatgaaa gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact
     2281 atcccaagcg acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac
     2341 taattctctg acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa
     2401 gtctaaaaga gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc
     2461 tttacggatt tttagtaaac cttgttcagg tctaacacta ccggtacccc atttaggacc
     2521 acccacagca cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg
     2581 aagtggaaca cctgtagcat cgatagcagc accaccaatt aaatgatttt cgaaatcgaa
     2641 cttgacattg gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat
     2701 ttcttgacca acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat
     2761 ggtatatcct tgaaatatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag
     2821 aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt
     2881 caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa
     2941 agccggttcc ggcgctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat
     3001 atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg
     3061 cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat
     3121 tcgaactctt gcatcttacg atacctgagt attcccacag ttaactgcgg tcaagatatt
     3181 tcttgaatca ggcgccttag accgctcggc caaacaacca attacttgtt gagaaataga
     3241 gtataattat cctataaata taacgttttt gaacacacat gaacaaggaa gtacaggaca
     3301 attgattttg aagagaatgt ggattttgat gtaattgttg ggattccatt tttaataagg
     3361 caataatatt aggtatgtag atatactaga agttctcctc gaccggtcga tatgcggtgt
     3421 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata
     3481 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3541 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3781 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3901 cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     3961 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     4021 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     4081 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac
     4141 tagtggatcc agtcattgag aatgaaaata taccattgag agatacaacc aatagattaa
     4201 aggtttctcc ttttgccatt tctaatcaag aggtgttaac cgctacaatg agcgaaaatg
     4261 gtgatgttta tatatacaat ctagctccac aaagcaaagc tttttccaca ccaggttatc
     4321 agattccgaa gtctgctaag cgtcctattc acactgtaaa aaatcatggg aatgttgaag
     4381 gctacgggtt ggattggtca ccattgatca agactggtgc gttactatca ggtgattgct
     4441 caggacaaat atattttacc caaaggcaca catcgagatg ggtgactgat aaacaaccat
     4501 ttactgtttc aaacaataaa tccatagaag atatccagtg gtctcgcact gaatccaccg
     4561 tttttgcaac cgcaggatgt gatggatata taaggatttg ggacacaaga tcaaaaaaac
     4621 ataaacctgc tatctctgtt aaagcttcta atactgacgt aaatgtcata agttggagtg
     4681 ataaaattgg ttacttgcta gcaagcggtg acgataacgg tacatgggga gtatgggatt
     4741 taagacagtt tacgccaagt aatgctgacg ccgtccaacc ggttgctcaa tatgacttcc
     4801 ataagggagc cattacttcc attgcattca acccattaga tgagtctatc gttgcggtag
     4861 gctcagaaga taatactgtg actttgtggg atttgtctgt agaagctgac gatgaggaaa
     4921 ttaaacaaca ggccgccgaa acaaaagagc tacaagaaat tccaccacaa ttattgtttg
     4981 ttcactggca aaaggaagtt aaagatgtca aatggcataa gcaaatccca ggttgtttag
     5041 taagtaccgg tactgacggt ttgaacgtct ggaaaacaat cagtgtctaa actgtataat
     5101 acttggatca tatatttaca catagtagat tattaataaa caaacccatt caaagatata
     5161 gtaaatatta ctggcggata tgaataatat ctatcataca tatttgttac tgtaacgtta
     5221 ggcgctcagg gaaagagttc ctctaaaacc tcaagattcg aaacaacgta ctttctttca
     5281 ctcaactgta acgtgtcagt ttgcttagta ttggcttccc aagtgtttat tgaattatta
     5341 tctattttat caattgacag cttgtattcg tcccgcattg gtttactaat cgagtttgaa
     5401 agaaacccgt atttatcatt tctatctaac agtataccag tccaaccggc agcttctgcc
     5461 ccttcgagat cgttctttaa ttcatctccg atgtgaaagc aatgctgtag aatttcctcc
     5521 ctggtgtact tttcgagtaa atgaggttgt ttagatataa tatcgtcaag cgcatattga
     5581 aatattgctc tatcaggttt ggcgagatta agctcatatg ataaataaat gtgaccggaa
     5641 aatgtctcaa ataatccgat attctttaat aatttgtaaa aaattggatc agtattgctt
     5701 actattccta agataacatc aggatgtctt gacttcaaat cctttaaaaa tttgatcaaa
     5761 tctggataaa caaaatacga atcgaagccc tcaaaccgca ttaatatttc atttatcatc
     5821 tcatcgggaa tttcgtttgg tgcgaagacg ttcctaatca agatggacca ccattgttcg
     5881 ggcttgatac cagaatattt tccgtattgg ggatagtctt ccttcagttt tttaaaaacg
     5941 tgtggaaaat tgtttgttaa agtcgaagga ttcgccttga taccatattt acgcccaaca
     6001 atgcagtatt gttccatgac gggaagtttt gtggcgtata gtgtattata tgcgtcgaaa
     6061 gtaataatta gtggccttgc caccctatga acttcagacc acgcatttat aggtattctt
     6121 ttcggatagg tcattgagcc aatttctgtg gatgttcaaa agtctgcttt taacacacct
     6181 ctatagaccg tcctttcggg ctttgttagc agccggatcc tttgtatagt tcatccatgc
     6241 catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt
     6301 tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag
     6361 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt
     6421 caatgttgtg tctaattttg aagttagctt tgattccatt cttttgtttg tctgccatga
     6481 tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg cccaagaatg tttccatctt
     6541 ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga
     6601 cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat
     6661 aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatcc gggtatcttg
     6721 aaaagcactg aacaccataa gtgaaagtag tgacaagtgt tggccatgga acaggtagtt
     6781 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
     6841 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
     6901 caactccagt gaagagttct tctcctttgc tagcaacgaa ctcgtgatcg cttgatatga
     6961 aaatgttcaa tagtagcata gctaaaatct ggagataaac aacccatggc ttaaaagtca
     7021 acctaggaat gttaaaggat ggtaaaacat acagggcggc ggcatctata atgaaatact
     7081 tggatatgaa attaaatcga tagttcttaa acagtaaacc cgaaagcggt aaccccgatt
     7141 taagtattac caggtcatat attttataac attgaattat taaaaacaat atcaccgcaa
     7201 atgtcctttg tttggaaatt tctaaaacat cggttgatat tagaggcagt gaataaatgt
     7261 ctaccttttt atcaccatta atgtagcggt ctttgctgtc acgttcagat atccttgatt
     7321 tttcatcgct tatgttactt tcagtacttt taaagctcct cgagcgtaaa ggtcggattt
     7381 tctcagcggg ctcatcgtca ctatattgga agcgtcgtgt tgtgtttaca ttaggacgac
     7441 tgctataaga tggacgtggt gatcctgaca cagaactgcc catccaatgg ttccctcgtg
     7501 gagtatcatt aaacacgtta tatctgtgct ccattataca agcctcctga taaacgtatc
     7561 tggtatgata aatctataat ccgttttgaa ttggttcgat gagtttcctc ttattatgtc
     7621 ttaagcaatt cttacttttg ttcaataaaa aaaacgtata aagttattga ctacgtatat
     7681 aatgacataa aatacatagt gctatagaac ataatgtttt gaattatgaa ggaagttaac
     7741 attaagtggt tgcaattatt tgatgcccta gataggaata atgtattctt cctatatgtt
     7801 cttatataat gaaactctat ttctataata ccagcgcatt attttttttc tttctcttca
     7861 cctgtgatgg tctggaagcc ctgatgaatc ccctaatgat ttttatcaaa atcattaagt
     7921 taaggtagat acacatcttg tcatatgatc aaatggtttc gccaaaaatc aataatcaga
     7981 caacaaaatg tgcgaactcg atattttaca cgactctctt taccaattct gccccgaatt
     8041 acacttaaaa cgactcaaca gcttaacgtt ggcttgccac gccttacttg actgtaaaac
     8101 tctcactctt accgaacttg gccgtaacct gccaaccaaa gcgagaacaa aacataacat
     8161 caaacgaatc gaccgattgt taggtaatcg tcacctccac aaagagcgac tcgctgtata
     8221 ccgttggcat gctagcttta tctgttcggg caatacgatg cccattgtac ttgttgactg
     8281 gtctgatatc cgtgagcaaa aacggcttat ggtattgcga gcttcagtcg cactacacgg
     8341 tcgttctgtt actctttatg agaaagcgtt cccgctttca gagcaatgtt caaagaaagc
     8401 tcatgaccaa tttctagccg accttgcgag cattctaccg agtaacacca caccgctcat
     8461 tgtcagtgat gctggcttta aagtgccatg gtataaatcc gttgagaagc tgggttggta
     8521 ctggttaagt cgagtaagag gaaaagtaca atatgcagac ctaggagcgg aaaactggaa
     8581 acctatcagc aacttacatg atatgtcatc tagtcactca aagactttag gctataagag
     8641 gctgactaaa agcaacccaa tctcatgcca aattctattg tataaatctc gctctaaagg
     8701 ccgaaaaaat cagcgctcga cacggactca ttgtcaccac ccgtcaccta aaatctactc
     8761 agcgtcggca aaggagccat ggattctagc aactaactta cctgttgaaa ttcgaacacc
     8821 caaacaactt gttaatatct attcgaagcg aatgcagatt gaagaaacct tccgagactt
     8881 gaaaagtcct gcctacggac taggcctacg ccatagccga acgagcagct cagagcgttt
     8941 tgatatcatg ctgctaatcg ccctgatgct tcaactaaca tgttggcttg cgggcgttca
     9001 tgctcagaaa caaggttggg acaagcactt ccaggctaac acagtcagaa atcgaaacgt
     9061 actctcaaca gttcgcttag gcatggaagt tttgcggcat tctggctaca caataacaag
     9121 ggaagactca ctcgtggctg caaccctgct tactcaaaat ctattcacac atggttacgt
     9181 tttggggaaa ttatgagggg atctctcagc tggaagccct ttctggtgat aaacttaatg
     9241 actgtccact ggcctttttg cttcttctga tgcactgcta taggatttaa atcccaacca
     9301 atcttgagat cgttgtcaac tgtggatccc ccgggctgca ggaattcgat atcaagctta
     9361 tcgataccgt cgacctcgag ggggggcccg gtaccagctt ttgttccctt tagtgagggt
     9421 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11161 actcaaccaa gtcattctga gaata
//
