LOCUS       sequence-368074-        9195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NMA111-GFP with NLS2 deleted under endogenous promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Belanger KD, Walter D, Henderson TA, Yelton AL, O'Brien TG, Belanger
            KG, Geier SJ, Fahrenkrog B
  TITLE     Nuclear localisation is crucial for the proapoptotic activity of the
            HtrA-like serine protease Nma111p.
  JOURNAL   J Cell Sci. 2009 Nov 1;122(Pt 21):3931-41. doi: 10.1242/jcs.056887. 
            Epub 2009 Oct 6.
  PUBMED    19808885
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2009-11-1"; volume: "122(Pt 21):3931-41. doi"; pages: 
            " 10.1242/jcs.056887. Epub 2009 Oct 6"
COMMENT     Sequence Label: KBB467
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        498..602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(603..671,672..1463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(821..840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1634..2222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2123..2142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2376..2393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2510..2531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2546..2551,2552..2569,2570..2576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2584..2600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2589..2611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2608..2624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2608..2624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2645..2663
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2691..2707
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2692..2708
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6074..6784
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6101..6129)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6724..6744
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6835..6853)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6849..6896
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7103..7122)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7104..7122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7129..7146)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7129..7145)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7138..7160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7286..7741
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7373..7392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7583..7604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7872..8675)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8676..8891)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8915..8934)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9034..9056
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9094..9112)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(9153..9195,1..461)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt
       61 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg
      121 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag
      181 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg
      241 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga
      301 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa
      361 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat
      421 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca
      481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      841 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1741 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2281 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2341 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2401 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2461 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2521 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2581 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     2641 tcgaaattaa ccctcactaa agggaacaaa agctggtacc gggccccccc tcgaggtcga
     2701 cggtatcgat aagcttgata tcgaattcct gcaggcatca gcatcagcaa gatccaagaa
     2761 cctagagtca cgattgaaat atttaataat aaatgctatg tatcatagat aaactagaaa
     2821 gaggcaagaa aaatttaaca agtttcttaa ttgaatatta ttttactgtc actaccatta
     2881 ttagacgcca tctcagcgtg tgacatgata atttttcttt ttttcttttt ttttcctcaa
     2941 ggcgatgata tgaaactttc aaaaaattta tatgtaaata aaaaagttct acgagggttt
     3001 aaatacggaa gtttatttag aagatattta tactctgaat acacacgtag agtacagtaa
     3061 aggtttttta gatctactaa tgaccatatc gttgagcaat ataaagaaaa gagaccattc
     3121 taaaatttcc gatggaactt ctggtgaatc atcgcttgta gcagcggcac agttggaaag
     3181 cgccaccgga gatcaagagg aagaatatac agaccatgaa atcattatag agccgttgca
     3241 ctttgctaat aataataaca ctgtactcac agattcagaa aattatctaa gatggcaaaa
     3301 taccatctcc aacgttgtta aatcagtggt ttccattcat ttttcacaag ttgcgccatt
     3361 cgattgcgat tctgctttgg tatctgaagc taccggattt gtcgtggatg ctaaattagg
     3421 tataattctg accaataggc acgtcgttgg tcctggtccg ttcgtaggct acgtagtttt
     3481 cgataatcat gaagaatgcg atgttatacc aatttacaga gatcctgttc atgattttgg
     3541 gttcctcaaa ttcgatccga aaaacataaa atattctaag atcaaagcgt taacgctaaa
     3601 accaagtttg gctaaggtcg gctcagaaat tagagttgtc ggtaatgatg ccggtgagaa
     3661 attaagtatc ctagcaggtt ttattagtag aattgacagg aacgctccag aatatggtga
     3721 gctgacatac aatgacttca atacagaata tattcaagct gctgcatctg cttctggcgg
     3781 ttcaagtggt tctccagtcg taaatataga tgggtatgct gtggctttac aggcaggtgg
     3841 ctctacagag gcatctactg attttttttt accgttagat agaattctta gagcgttaat
     3901 ttgcatccaa accaacaaac ctatcacacg tggtacgatt caagttcaat ggctgctgaa
     3961 gccttatgac gagtgtagaa gattaggttt aacttcagag agagaaagtg aggctcgtgc
     4021 taaatttccc gaaaatatcg gactgttggt agcagaaacc gttttaagag aaggcccagg
     4081 ctatgataaa attaaagaag gtgatactct aatttcaata aacggtgaaa ccatatcttc
     4141 tttcatgcaa gtggataaaa ttcaagatga aaatgttggg aaggaaatcc aattggttat
     4201 tcaaaggggc ggtgtcgaat gtactgtcac ctgcactgtt ggtgatttac atgctatcac
     4261 ccctcatcgt tatgtagagg tttgtggtgc gacttttcat gaattatcat atcaaatggc
     4321 aaggttttat gcgctacctg tcaggggtgt atttttaagt agtgcatcag ggtctttcaa
     4381 ctttgattca aaagaaagag tgggttggat tgtggattct atagacaata aggaaacccc
     4441 cgacttagat acatttattg aaattatgaa aactattcca gatcgtaagc gcgttactgt
     4501 acgataccac catctaacag atcagcattc tccacttgtt acttctattt acattgaccg
     4561 tcattggtgc aatgaattca gagtttacac aaggaacgat acaactggta tttgggatta
     4621 caaaaatgtt gctgatccac ttccagccga tgcgttgaaa cctcgttcgg ccaaaattat
     4681 tcctattcct gttaataatg aaaaagttgc aaaattgtca tcatcgttat gtacagtggc
     4741 tacaatggca gcagttccat tggattcact ttctgctgat attttaaaaa catcaggctt
     4801 aatcattgat gctgaaaaag gttatgtcct agtgtccaga agggtagttc cacatgattg
     4861 tcttgatact tttgtcacca ttgcagattc ccttgtagtt ccagccacag tagaatttct
     4921 ccatccaact cacaacttcg ctattgttaa atatgatcca gagttagtaa aggctccact
     4981 aatcactccc aagctatcta ccaccagaat gaaacgtggt gataagcttc agtttatagg
     5041 ttttacccag aatgatagaa ttgttacttc agagaccact gtcaccgata tttcctctgt
     5101 aagtattcca agtaatttaa ttccgaggta ccgtgccact aatttagaag ctatatctat
     5161 cgactgcaac gttagcacca gatgtaattc cggtattttg acagataatg atggtacagt
     5221 aagaggttta tggcttccat ttttaggcga aagattagaa aataaagaaa aggtttattt
     5281 gatgggatta gatataatgg actgtaggga agttatagat atcttaaaaa atggcggtaa
     5341 accaagagta agtatagttg atgctgggtt cggttcaatt tccgttctgc aagccagaat
     5401 tagaggtgtg cccgaagaat ggatcatgcg tatggaacat gaatcaaata acaggctaca
     5461 gtttatcact gtttctagag tttcttatac agaagacaag attcatttag agaccggtga
     5521 cgtcatttta tctgtgaacg gaaaattagt cactgaaatg aatgatttga atggagttgt
     5581 aagctcagct gatggtattt tgccatcagc catgttagat tttaaagttg ttcgtgatgg
     5641 taatattgtt gatttgaaaa ttaaaaccgt ggaagttcag gaaaccgacc gttttgtaat
     5701 atttgcaggt agtatcttac aaaagccgca tcatgctgtt ttgcaagcta tggttgatgt
     5761 tccaaagggt gtctactgta cattcagggg agaatcgtca ccggcgctcc agtacggtat
     5821 atctgccaca aacttcataa ctcatgtcaa tgaaattgaa acacctgatc tagatacatt
     5881 tttgaaagtt gtcaaaacaa tccctgataa tagttattgt aaaatgaggt taatgacttt
     5941 cgacaatgtg cccttcgcta tttcactgaa aacgaattat cattacttcc ctactgcaga
     6001 attgaagagg gacaacatca cgcacaaatg gattgaaaag gaatttaccg gcaacagcca
     6061 aagtgaaaaa gctagcaaag gagaagaact cttcactgga gttgtcccaa ttcttgttga
     6121 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     6181 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     6241 gccaacactt gtcactactt tcacttatgg tgttcagtgc ttttcaagat acccggatca
     6301 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     6361 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     6421 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     6481 tgggcacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     6541 aaagaatgga atcaaagcta acttcaaaat tagacacaac attgaagatg gaagcgttca
     6601 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     6661 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     6721 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     6781 caaaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     6841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     6901 aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg
     6961 atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt
     7021 gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag agcggccgcc
     7081 accgcggtgg agctccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
     7141 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7201 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7261 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7321 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7381 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7441 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7501 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7561 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7621 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7681 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7741 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat
     7801 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     7861 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
     7921 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
     7981 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
     8041 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
     8101 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
     8161 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat
     8221 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa
     8281 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
     8341 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
     8401 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
     8461 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
     8521 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
     8581 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
     8641 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt
     8701 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     8761 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     8821 attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     8881 gatgaattga aaaggtggta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8941 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9001 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9061 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     9121 ttaatgtcat gataataatg gtttcttaga cggatcgctt gcctgtaact tacacgcgcc
     9181 tcgtatcttt taatg
//
