LOCUS       sequence-368075-        9195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NMA111-GFP with NLS1 and NLS2 deleted under endogenous promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Belanger KD, Walter D, Henderson TA, Yelton AL, O'Brien TG, Belanger
            KG, Geier SJ, Fahrenkrog B
  TITLE     Nuclear localisation is crucial for the proapoptotic activity of the
            HtrA-like serine protease Nma111p.
  JOURNAL   J Cell Sci. 2009 Nov 1;122(Pt 21):3931-41. doi: 10.1242/jcs.056887. 
            Epub 2009 Oct 6.
  PUBMED    19808885
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2009-11-1"; volume: "122(Pt 21):3931-41. doi"; pages: 
            " 10.1242/jcs.056887. Epub 2009 Oct 6"
COMMENT     Sequence Label: KBB466
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     178..197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     431..448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    565..586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(601..606,607..624,625..631)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    639..655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     644..666
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     663..679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     663..679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        700..718
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     746..762
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     747..763
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4129..4839
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4156..4184)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4779..4799
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(4890..4908)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4904..4951
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(5158..5177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5159..5177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5184..5201)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5184..5200)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5193..5215)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5341..5796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5428..5447)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5638..5659
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5927..6730)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6731..6946)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6970..6989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7089..7111
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7149..7167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7208..7711
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        7748..7852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7853..7921,7922..8713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8071..8090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8884..9195,1..277)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
       61 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      301 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      361 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      421 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      481 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
      541 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      601 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      661 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
      721 acaaaagctg gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa
      781 ttcctgcagg catcagcatc agcaagatcc aagaacctag agtcacgatt gaaatattta
      841 ataataaatg ctatgtatca tagataaact agaaagaggc aagaaaaatt taacaagttt
      901 cttaattgaa tattatttta ctgtcactac cattattaga cgccatctca gcgtgtgaca
      961 tgataatttt tctttttttc tttttttttc ctcaaggcga tgatatgaaa ctttcaaaaa
     1021 atttatatgt aaataaaaaa gttctacgag ggtttaaata cggaagttta tttagaagat
     1081 atttatactc tgaatacaca cgtagagtac agtaaaggtt ttttagatct actaatgacc
     1141 atatcgttga gcaatatagc ggcagcagac cattctaaaa tttccgatgg aacttctggt
     1201 gaatcatcgc ttgtagcagc ggcacagttg gaaagcgcca ccggagatca agaggaagaa
     1261 tatacagacc atgaaatcat tatagagccg ttgcactttg ctaataataa taacactgta
     1321 ctcacagatt cagaaaatta tctaagatgg caaaatacca tctccaacgt tgttaaatca
     1381 gtggtttcca ttcatttttc acaagttgcg ccattcgatt gcgattctgc tttggtatct
     1441 gaagctaccg gatttgtcgt ggatgctaaa ttaggtataa ttctgaccaa taggcacgtc
     1501 gttggtcctg gtccgttcgt aggctacgta gttttcgata atcatgaaga atgcgatgtt
     1561 ataccaattt acagagatcc tgttcatgat tttgggttcc tcaaattcga tccgaaaaac
     1621 ataaaatatt ctaagatcaa agcgttaacg ctaaaaccaa gtttggctaa ggtcggctca
     1681 gaaattagag ttgtcggtaa tgatgccggt gagaaattaa gtatcctagc aggttttatt
     1741 agtagaattg acaggaacgc tccagaatat ggtgagctga catacaatga cttcaataca
     1801 gaatatattc aagctgctgc atctgcttct ggcggttcaa gtggttctcc agtcgtaaat
     1861 atagatgggt atgctgtggc tttacaggca ggtggctcta cagaggcatc tactgatttt
     1921 tttttaccgt tagatagaat tcttagagcg ttaatttgca tccaaaccaa caaacctatc
     1981 acacgtggta cgattcaagt tcaatggctg ctgaagcctt atgacgagtg tagaagatta
     2041 ggtttaactt cagagagaga aagtgaggct cgtgctaaat ttcccgaaaa tatcggactg
     2101 ttggtagcag aaaccgtttt aagagaaggc ccaggctatg ataaaattaa agaaggtgat
     2161 actctaattt caataaacgg tgaaaccata tcttctttca tgcaagtgga taaaattcaa
     2221 gatgaaaatg ttgggaagga aatccaattg gttattcaaa ggggcggtgt cgaatgtact
     2281 gtcacctgca ctgttggtga tttacatgct atcacccctc atcgttatgt agaggtttgt
     2341 ggtgcgactt ttcatgaatt atcatatcaa atggcaaggt tttatgcgct acctgtcagg
     2401 ggtgtatttt taagtagtgc atcagggtct ttcaactttg attcaaaaga aagagtgggt
     2461 tggattgtgg attctataga caataaggaa acccccgact tagatacatt tattgaaatt
     2521 atgaaaacta ttccagatcg taagcgcgtt actgtacgat accaccatct aacagatcag
     2581 cattctccac ttgttacttc tatttacatt gaccgtcatt ggtgcaatga attcagagtt
     2641 tacacaagga acgatacaac tggtatttgg gattacaaaa atgttgctga tccacttcca
     2701 gccgatgcgt tgaaacctcg ttcggccaaa attattccta ttcctgttaa taatgaaaaa
     2761 gttgcaaaat tgtcatcatc gttatgtaca gtggctacaa tggcagcagt tccattggat
     2821 tcactttctg ctgatatttt aaaaacatca ggcttaatca ttgatgctga aaaaggttat
     2881 gtcctagtgt ccagaagggt agttccacat gattgtcttg atacttttgt caccattgca
     2941 gattcccttg tagttccagc cacagtagaa tttctccatc caactcacaa cttcgctatt
     3001 gttaaatatg atccagagtt agtaaaggct ccactaatca ctcccaagct atctaccacc
     3061 agaatgaaac gtggtgataa gcttcagttt ataggtttta cccagaatga tagaattgtt
     3121 acttcagaga ccactgtcac cgatatttcc tctgtaagta ttccaagtaa tttaattccg
     3181 aggtaccgtg ccactaattt agaagctata tctatcgact gcaacgttag caccagatgt
     3241 aattccggta ttttgacaga taatgatggt acagtaagag gtttatggct tccattttta
     3301 ggcgaaagat tagaaaataa agaaaaggtt tatttgatgg gattagatat aatggactgt
     3361 agggaagtta tagatatctt aaaaaatggc ggtaaaccaa gagtaagtat agttgatgct
     3421 gggttcggtt caatttccgt tctgcaagcc agaattagag gtgtgcccga agaatggatc
     3481 atgcgtatgg aacatgaatc aaataacagg ctacagttta tcactgtttc tagagtttct
     3541 tatacagaag acaagattca tttagagacc ggtgacgtca ttttatctgt gaacggaaaa
     3601 ttagtcactg aaatgaatga tttgaatgga gttgtaagct cagctgatgg tattttgcca
     3661 tcagccatgt tagattttaa agttgttcgt gatggtaata ttgttgattt gaaaattaaa
     3721 accgtggaag ttcaggaaac cgaccgtttt gtaatatttg caggtagtat cttacaaaag
     3781 ccgcatcatg ctgttttgca agctatggtt gatgttccaa agggtgtcta ctgtacattc
     3841 aggggagaat cgtcaccggc gctccagtac ggtatatctg ccacaaactt cataactcat
     3901 gtcaatgaaa ttgaaacacc tgatctagat acatttttga aagttgtcaa aacaatccct
     3961 gataatagtt attgtaaaat gaggttaatg actttcgaca atgtgccctt cgctatttca
     4021 ctgaaaacga attatcatta cttccctact gcagaattga agagggacaa catcacgcac
     4081 aaatggattg aaaaggaatt taccggcaac agccaaagtg aaaaagctag caaaggagaa
     4141 gaactcttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     4201 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     4261 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcact
     4321 tatggtgttc agtgcttttc aagatacccg gatcatatga aacggcatga ctttttcaag
     4381 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     4441 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     4501 aaaggtattg attttaaaga agatggaaac attcttgggc acaaattgga atacaactat
     4561 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agctaacttc
     4621 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     4681 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     4741 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     4801 gctgctggga ttacacatgg catggatgaa ctatacaaag gatccggctg ctaacaaagc
     4861 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     4921 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
     4981 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
     5041 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
     5101 attgcatcaa cgcatatagc gctagagcgg ccgccaccgc ggtggagctc caattcgccc
     5161 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5221 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5281 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5341 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5401 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5461 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5521 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5581 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5641 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5701 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5761 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     5821 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
     5881 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
     5941 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
     6001 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
     6061 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
     6121 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
     6181 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
     6241 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
     6301 gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
     6361 caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
     6421 gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
     6481 acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
     6541 tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
     6601 attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
     6661 tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
     6721 ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga
     6781 atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc
     6841 tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaag
     6901 gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagg tggtatggtg
     6961 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     7021 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7081 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     7141 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7201 ttagacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg atggaataat
     7261 ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt tagaaagtaa
     7321 ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat
     7381 ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga
     7441 tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc
     7501 tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga gcaagataaa
     7561 aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat
     7621 tttttcttta atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat
     7681 ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg
     7741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8101 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8161 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8221 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8281 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8341 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8401 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8461 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8521 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8581 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8641 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8701 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9001 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9181 taaggcgcag cggtc
//
