LOCUS       sequence-368079-        4785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF800 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF800
FEATURES             Location/Qualifiers
     source          1..4785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2096..2195)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2212..2231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2213..2231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2236..2252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2236..2252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2683..3474
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3567..4155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4056..4075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4309..4326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4485..4512)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4604..4690)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4739..4761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4753..4770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4754..4770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatgccttt aagggacaaa
      121 tactgtcaga ctgaccacca tcatcacgga tgctgtgaac cagtttatat cctggaacct
      181 ggagatcctc ctttgttaca gcaaccacta cagacatcca aatctggtat tcaacaaata
      241 attgagtgct ttcgatcagg aactaaacaa cttaagcata ttttattaaa agatgtggac
      301 actatttttg aatgtaagtt atgccgcagt ctcttcagag gattaccaaa tttaattacc
      361 cataaaaaat tctactgccc accaagtctc cagatggatg acaaccttcc tgatgtaaat
      421 gataaacaaa gccaagccat aaatgatctc ctagaagcca tatatccaag tgtggacaaa
      481 cgagaatata ttattaagct agaacccata gaaactaatc aaaatgcagt atttcaatat
      541 atttcgagga ctgataatcc tattgaagtc acagagtcaa gcagtactcc tgaacaaacc
      601 gaagttcaga tacaggaaac tagcactgaa cagtcaaaaa cagtaccggt tacagataca
      661 gaggtggaaa ctgtagagcc ccctcctgtt gagattgtta cagatgaagt tgcacctaca
      721 tctgatgaac aacctcagga gtcgcaggct gacttggaaa cttctgacaa ttctgatttt
      781 ggtcaccagt tgatatgttg tctttgtaga aaagaattca attctagacg aggtgttcgc
      841 cgtcacattc gaaaagtaca caagaaaaag atggaagaac taaaaaagta cattgaaaca
      901 cgaaagaatc caaaccaatc ctctaaagga cgcagtaaga atgttctagt tccattaagt
      961 aggagttgtc cagtatgttg taaatcattt gctacaaaag cgaatgtaag gaggcatttt
     1021 gatgaagttc atagaggact aaggagggat tcaattactc ctgatatagc aacaaagcct
     1081 gggcaacctt tgttcctgga ttctatttct cctaaaaaat cttttaagac tcgaaaacaa
     1141 aagtcttctt caaaggctga atacaattta actgcatgca aatgcctcct ttgcaagagg
     1201 aaatatagtt cacaaataat gcttaaaaga catatgcaaa ttgtccacaa gataactctt
     1261 tctggaacaa actctaaaag agaaaaaggc cctaataata ctgccaacag ttcagaaata
     1321 aaagttaaag ttgaaccagc agattctgta gaatcttcac ccccttccat tacccattct
     1381 ccacagaatg aattaaaggg aacaaatcat tcaaatgaaa aaaagaacac accggcagca
     1441 cagaaaaata aagttaaaca agactctgaa agccctaaat caactagtcc gtcggctgca
     1501 ggtggccagc aaaaaaccag aaaaccaaaa ctttcagctg gctttgactt taagcaactt
     1561 tactgtaaac tttgtaaacg tcagtttact tccaaacaga acttgactaa acacatcgag
     1621 ttgcacacag atggaaataa catttatgtt aaattctaca agtgtcctct ttgcacttat
     1681 gaaactcgtc ggaaacgtga tgtgatacga catataactg tggttcataa aaagtcatct
     1741 cgttatcttg ggaaaataac agccagttta gagatcagag ctataaaaaa gcctattgat
     1801 tttgttctaa ataaagtggc aaaaagaggc ccttcgaggg atgaagcaaa acatagtgat
     1861 tcaaaacatg atggcacttc taactctcct agtaaaaagt atgaagtagc tgacgtcggt
     1921 attgaagtaa aagtcacaaa aaacttttct cttcacagat gcaataaatg tggaaaggca
     1981 tttgccaaaa agacttacct tgaacatcat aagaaaactc ataaggcaaa tgcttccaat
     2041 tcacctgaag gaaacaaaac caaaggccga agtacaagat ctaaggctct tgtcttgcca
     2101 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2161 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2221 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2281 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     2341 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     2401 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     2461 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2521 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     2581 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2641 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     2701 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     2761 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     2821 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2881 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2941 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     3001 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     3061 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     3121 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     3181 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3241 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3301 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     3361 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     3421 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     3481 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3541 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3601 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3661 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3721 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3781 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3841 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3901 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3961 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4021 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4081 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4141 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4201 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4261 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4321 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4381 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4441 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4501 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4561 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4621 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4681 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4741 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
