LOCUS       sequence-368083-        9195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NMA111-GFP with NLS1 deleted under endogenous promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Belanger KD, Walter D, Henderson TA, Yelton AL, O'Brien TG, Belanger
            KG, Geier SJ, Fahrenkrog B
  TITLE     Nuclear localisation is crucial for the proapoptotic activity of the
            HtrA-like serine protease Nma111p.
  JOURNAL   J Cell Sci. 2009 Nov 1;122(Pt 21):3931-41. doi: 10.1242/jcs.056887. 
            Epub 2009 Oct 6.
  PUBMED    19808885
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2009-11-1"; volume: "122(Pt 21):3931-41. doi"; pages: 
            " 10.1242/jcs.056887. Epub 2009 Oct 6"
COMMENT     Sequence Label: KBB465
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        355..459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(460..528,529..1320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(678..697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1491..2079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1980..1999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2233..2250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2367..2388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2403..2408,2409..2426,2427..2433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2441..2457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2446..2468
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2465..2481
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2465..2481
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2502..2520
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2548..2564
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2549..2565
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5931..6641
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5958..5986)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6581..6601
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6692..6710)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6706..6753
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(6960..6979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6961..6979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6986..7003)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6986..7002)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6995..7017)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7143..7598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7230..7249)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7440..7461
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7729..8532)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8533..8748)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8772..8791)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8891..8913
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8951..8969)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(9010..9195,1..318)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
       61 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
      121 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
      181 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt
      241 tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt
      301 atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa tgtgcgcgga
      361 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      421 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      481 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      541 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      601 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      661 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      721 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      781 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      841 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      901 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      961 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1021 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1081 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1141 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1201 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1261 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1321 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1381 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1441 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1501 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1561 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1621 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1681 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1741 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1801 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1861 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1921 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1981 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2041 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2101 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2161 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2221 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2281 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2341 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     2401 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     2461 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
     2521 gaacaaaagc tggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
     2581 aattcctgca ggcatcagca tcagcaagat ccaagaacct agagtcacga ttgaaatatt
     2641 taataataaa tgctatgtat catagataaa ctagaaagag gcaagaaaaa tttaacaagt
     2701 ttcttaattg aatattattt tactgtcact accattatta gacgccatct cagcgtgtga
     2761 catgataatt tttctttttt tctttttttt tcctcaaggc gatgatatga aactttcaaa
     2821 aaatttatat gtaaataaaa aagttctacg agggtttaaa tacggaagtt tatttagaag
     2881 atatttatac tctgaataca cacgtagagt acagtaaagg ttttttagat ctactaatga
     2941 ccatatcgtt gagcaatata gcggcagcag accattctaa aatttccgat ggaacttctg
     3001 gtgaatcatc gcttgtaaaa aggaaacagt tggaaagcgc caccggagat caagaggaag
     3061 aatatacaga ccatgaaatc attatagagc cgttgcactt tgctaataat aataacactg
     3121 tactcacaga ttcagaaaat tatctaagat ggcaaaatac catctccaac gttgttaaat
     3181 cagtggtttc cattcatttt tcacaagttg cgccattcga ttgcgattct gctttggtat
     3241 ctgaagctac cggatttgtc gtggatgcta aattaggtat aattctgacc aataggcacg
     3301 tcgttggtcc tggtccgttc gtaggctacg tagttttcga taatcatgaa gaatgcgatg
     3361 ttataccaat ttacagagat cctgttcatg attttgggtt cctcaaattc gatccgaaaa
     3421 acataaaata ttctaagatc aaagcgttaa cgctaaaacc aagtttggct aaggtcggct
     3481 cagaaattag agttgtcggt aatgatgccg gtgagaaatt aagtatccta gcaggtttta
     3541 ttagtagaat tgacaggaac gctccagaat atggtgagct gacatacaat gacttcaata
     3601 cagaatatat tcaagctgct gcatctgctt ctggcggttc aagtggttct ccagtcgtaa
     3661 atatagatgg gtatgctgtg gctttacagg caggtggctc tacagaggca tctactgatt
     3721 tttttttacc gttagataga attcttagag cgttaatttg catccaaacc aacaaaccta
     3781 tcacacgtgg tacgattcaa gttcaatggc tgctgaagcc ttatgacgag tgtagaagat
     3841 taggtttaac ttcagagaga gaaagtgagg ctcgtgctaa atttcccgaa aatatcggac
     3901 tgttggtagc agaaaccgtt ttaagagaag gcccaggcta tgataaaatt aaagaaggtg
     3961 atactctaat ttcaataaac ggtgaaacca tatcttcttt catgcaagtg gataaaattc
     4021 aagatgaaaa tgttgggaag gaaatccaat tggttattca aaggggcggt gtcgaatgta
     4081 ctgtcacctg cactgttggt gatttacatg ctatcacccc tcatcgttat gtagaggttt
     4141 gtggtgcgac ttttcatgaa ttatcatatc aaatggcaag gttttatgcg ctacctgtca
     4201 ggggtgtatt tttaagtagt gcatcagggt ctttcaactt tgattcaaaa gaaagagtgg
     4261 gttggattgt ggattctata gacaataagg aaacccccga cttagataca tttattgaaa
     4321 ttatgaaaac tattccagat cgtaagcgcg ttactgtacg ataccaccat ctaacagatc
     4381 agcattctcc acttgttact tctatttaca ttgaccgtca ttggtgcaat gaattcagag
     4441 tttacacaag gaacgataca actggtattt gggattacaa aaatgttgct gatccacttc
     4501 cagccgatgc gttgaaacct cgttcggcca aaattattcc tattcctgtt aataatgaaa
     4561 aagttgcaaa attgtcatca tcgttatgta cagtggctac aatggcagca gttccattgg
     4621 attcactttc tgctgatatt ttaaaaacat caggcttaat cattgatgct gaaaaaggtt
     4681 atgtcctagt gtccagaagg gtagttccac atgattgtct tgatactttt gtcaccattg
     4741 cagattccct tgtagttcca gccacagtag aatttctcca tccaactcac aacttcgcta
     4801 ttgttaaata tgatccagag ttagtaaagg ctccactaat cactcccaag ctatctacca
     4861 ccagaatgaa acgtggtgat aagcttcagt ttataggttt tacccagaat gatagaattg
     4921 ttacttcaga gaccactgtc accgatattt cctctgtaag tattccaagt aatttaattc
     4981 cgaggtaccg tgccactaat ttagaagcta tatctatcga ctgcaacgtt agcaccagat
     5041 gtaattccgg tattttgaca gataatgatg gtacagtaag aggtttatgg cttccatttt
     5101 taggcgaaag attagaaaat aaagaaaagg tttatttgat gggattagat ataatggact
     5161 gtagggaagt tatagatatc ttaaaaaatg gcggtaaacc aagagtaagt atagttgatg
     5221 ctgggttcgg ttcaatttcc gttctgcaag ccagaattag aggtgtgccc gaagaatgga
     5281 tcatgcgtat ggaacatgaa tcaaataaca ggctacagtt tatcactgtt tctagagttt
     5341 cttatacaga agacaagatt catttagaga ccggtgacgt cattttatct gtgaacggaa
     5401 aattagtcac tgaaatgaat gatttgaatg gagttgtaag ctcagctgat ggtattttgc
     5461 catcagccat gttagatttt aaagttgttc gtgatggtaa tattgttgat ttgaaaatta
     5521 aaaccgtgga agttcaggaa accgaccgtt ttgtaatatt tgcaggtagt atcttacaaa
     5581 agccgcatca tgctgttttg caagctatgg ttgatgttcc aaagggtgtc tactgtacat
     5641 tcaggggaga atcgtcaccg gcgctccagt acggtatatc tgccacaaac ttcataactc
     5701 atgtcaatga aattgaaaca cctgatctag atacattttt gaaagttgtc aaaacaatcc
     5761 ctgataatag ttattgtaaa atgaggttaa tgactttcga caatgtgccc ttcgctattt
     5821 cactgaaaac gaattatcat tacttcccta ctgcagaatt gaagagggac aacatcacgc
     5881 acaaatggat tgaaaaggaa tttaccggca acagccaaag tgaaaaagct agcaaaggag
     5941 aagaactctt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     6001 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     6061 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttca
     6121 cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacggcat gactttttca
     6181 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     6241 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     6301 taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg gaatacaact
     6361 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagctaact
     6421 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     6481 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     6541 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     6601 cagctgctgg gattacacat ggcatggatg aactatacaa aggatccggc tgctaacaaa
     6661 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     6721 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
     6781 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
     6841 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     6901 aaattgcatc aacgcatata gcgctagagc ggccgccacc gcggtggagc tccaattcgc
     6961 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7021 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7081 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7141 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7201 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7261 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7321 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7381 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7441 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7501 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7561 taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt
     7621 acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc
     7681 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     7741 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     7801 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     7861 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     7921 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     7981 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     8041 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
     8101 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     8161 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     8221 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     8281 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     8341 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     8401 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     8461 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     8521 agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa
     8581 gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
     8641 tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
     8701 aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg
     8761 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     8821 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8881 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8941 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     9001 tcttagacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
     9061 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
     9121 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
     9181 atttcaacaa aaagc
//
