LOCUS       sequence-36809-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Yoon HJ, Kim HL, Lee SK, Kim HW, Kim HW, Lee JY, Mikami B, Suh SW
  TITLE     Crystal structure of peptide deformylase from Staphylococcus aureus 
            in complex with actinonin, a naturally occurring antibacterial 
            agent.
  JOURNAL   Proteins. 2004 Nov 15. 57(3):639-42.
  PUBMED    15382235
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2004 Nov 15. 57(3):639-42."
COMMENT     Sequence Label: SAPDF-pET21a(+)
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             607..629
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    644..668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(668..687)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(669..687)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(771..790)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttcgggcttg tagcagccgg atctcagtgg tggtggtggt ggtgctcgag aacttctact
       61 gcatctgtat gtggttgtaa tgggtgatct ttgtcaatgt gatcatagaa cattacacca
      121 tttaaatggt caatttcatg ttggaaaaca attgctggat atccttttag tcgtaattgt
      181 atatcattac cttcgatgtc tttggcttta attgtaattc tattatgacg gtgaactaga
      241 ccagcaacat tatcatcgac actaaggcaa ccttcaccag ttggtaaata agcttcttga
      301 acgctatgac ttacaatttt tgggttcaca agcatatagt cataagattt gccactgcca
      361 tcatctggta ttaaaacagc aatcatacgt ttagaaatat taatttgagg tgcagccaaa
      421 ccaacgcctg aacgtaaacc atatcgtttc gcgatttcct catcttgact atttactaaa
      481 aactctctca tggcgattaa tgtttctttt tcttctttag ttaatggtaa ttctaactca
      541 gctgcttttt gacgcaaagt tggatgacca tcgctaatga tgtctttcat tgttaacata
      601 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
      661 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc
      721 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      781 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
      841 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
      901 accattcctt gcggcggcgg tgctca
//
