LOCUS       sequence-368102-        2603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TT4
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     427..443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     427..443
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     448..467
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        448..466
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(641..1831)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     1460..1479
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(1879..1884,1885..1901,1902..1907))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(join(2034..2603,1..19))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2114..2133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
       61 gaacgacgcg taactcacgt taagggattt tggtcatggg tggctcgacc agatatggtc
      121 tcattgctac aacttgctcg ggcgtcaggc cgtgggcttg acagagaact ggtagaaggc
      181 gctgaacagt ctcaagagcc tgtttgccac ccccattact agcaatcgct acaacttgct
      241 cgggcgtcag gccgtgggct tgacagagaa ctggtagaag gcgctgaaca gtctcaagag
      301 cctgtttgcc acccccatta ctagcaatcg ctaccgagac cagctttggt ctagaggagg
      361 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag
      421 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat attttatttt
      481 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
      541 gatcaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt
      601 ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc
      661 tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc
      721 atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc
      781 caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt
      841 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
      901 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
      961 agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     1021 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     1081 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     1141 ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga
     1201 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatgg
     1261 gtcggcgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     1321 cgttgagcac cgccgccgca aggaatggtg catgtaagga gatggcgccc aacagtcccc
     1381 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     1441 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     1501 cgccggtgat gccggccacg atgcgtccgg cgtagagaat ccacaggacg ggtgtggtcg
     1561 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc
     1621 caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata
     1681 gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga
     1741 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     1801 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
     1861 actgtgataa actaccgcat taaagctaat cgatgataag ctgtcaaaca tgagaattaa
     1921 ttcatgttct ttcctgcgtt atcccctgat tctgtggata acgcaggaaa gaacatgtga
     1981 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2041 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2101 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2161 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2221 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2281 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2341 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2401 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2461 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2521 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2581 gtttgcaagc agcagattac gcg
//
