LOCUS       sequence-368106-       11185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused 
            to HA and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Brown JT, Haraczy AJ, Wilhelm CM, Belanger KD
  TITLE     Characterization of nuclear pore complex targeting domains in Pom152
            in Saccharomyces cerevisiae.
  JOURNAL   Biol Open. 2021 Oct 15;10(10). pii: 272274. doi: 10.1242/bio.057661.
            Epub 2021 Oct 20.
  PUBMED    34557894
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2021-10-15"; volume: "10(10). pii: 272274. doi"; 
            pages: " 10.1242/bio.057661. Epub 2021 Oct 20"
COMMENT     Sequence Label: KBB520
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      750..1338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1239..1258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1492..1509
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1626..1647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1662..1667,1668..1685,1686..1692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1700..1716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1705..1727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1724..1740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1724..1740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1761..1779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1807..1823
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1808..1824
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4255..4965
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4282..4310)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4905..4925
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7037..7053)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7037..7053)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7085..7104)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7086..7104)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7111..7128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7111..7127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7120..7142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7268..7723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7355..7374)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7565..7586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8024..8431
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8432..9526
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(10020..10039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10139..10161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10199..10217)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    10259..10762
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10799..10903
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10904..10972,10973..11185,1..579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11122..11141)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
       61 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      121 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      181 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      241 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      301 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      361 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
      421 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
      481 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
      541 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
      601 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
      661 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      721 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      781 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      841 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      901 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      961 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1021 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1081 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1141 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1201 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1261 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1321 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1381 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     1441 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     1501 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     1561 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     1621 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     1681 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     1741 catgattacg ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc
     1801 cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
     1861 ccacagttga caacgatctc aagattggtt gggatttaaa tcctatagca gtgcatcaga
     1921 agaagcaaaa aggccagtgg acagtcatta agtttatcac cagaaagggc ttccagctga
     1981 gagatcccct cataatttcc ccaaaacgta accatgtgtg aatagatttt gagtaagcag
     2041 ggttgcagcc acgagtgagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc
     2101 catgcctaag cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg
     2161 cttgtcccaa ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat
     2221 cagggcgatt agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag
     2281 gcctagtccg taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt
     2341 cgaatagata ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag
     2401 aatccatggc tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt
     2461 ccgtgtcgag cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca
     2521 tgagattggg ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga
     2581 catatcatgt aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac
     2641 ttttcctctt actcgactta accagtacca acccagcttc tcaacggatt tataccatgg
     2701 cactttaaag ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc
     2761 aaggtcggct agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc
     2821 tttctcataa agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag
     2881 ccgtttttgc tcacggatat cagaccagtc aacaagtaca atgggcatcg tattgcccga
     2941 acagataaag ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt
     3001 acctaacaat cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt
     3061 acggccaagt tcggtaagag tgagagtttt acagtcaagt aaggcgtggc aagccaacgt
     3121 taagctgttg agtcgtttta agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa
     3181 aatatcgagt tcgcacattt tgttgtctga ttattgattt ttggcgaaac catttgatca
     3241 tatgacaaga tgtgtatcta ccttaactta atgattttga taaaaatcat taggggattc
     3301 atcagggctt ccagaccatc acaggtgaag agaaagaaaa aaaataatgc gctggtatta
     3361 tagaaataga gtttcattat ataagaacat ataggaagaa tacattattc ctatctaggg
     3421 catcaaataa ttgcaaccac ttaatgttaa cttccttcat aattcaaaac attatgttct
     3481 atagcactat gtattttatg tcattatata cgtagtcaat aactttatac gtttttttta
     3541 ttgaacaaaa gtaagaattg cttaagacat aataagagga aactcatcga accaattcaa
     3601 aacggattat agatttatca taccagatac gtttatcagg aggcttgtat aatggagcac
     3661 agatataacg tgtttaatga tactccacga gggaaccatt ggatgggcag ttctgtgtca
     3721 ggatcaccac gtccatctta tagcagtcgt cctaatgtaa acacaacacg acgcttccaa
     3781 tatagtgacg atgagcccgc tgagaaaatc cgacctttac gctcgaggag ctttaaaagt
     3841 actgaaagta acataagcga tgaaaaatca aggatatctg aacgtgacag caaagaccgc
     3901 tacattaatg gtgataaaaa ggtagacatt tattcactgc ctctaatatc aaccgatgtt
     3961 ttagaaattt ccaaacaaag gacatttgcg gtgatattgt ttttaataat tcaatgttat
     4021 aaaatatatg acctggtaat acttaaatcg gggttaccgc tttcgggttt actgtttaag
     4081 aactatcgat ttaatttcat atccaagtat ttcattatag attccttctt cctgtatgtt
     4141 ttaccatcct ttaacattcc taggttgact tttaagccat gggttgttta tctccagatt
     4201 ttagctatgc tactattgaa cattttcata tcaagcgatc acgagttcgt tgctagcaaa
     4261 ggagaagaac tcttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     4321 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     4381 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     4441 ttcacttatg gtgttcagtg cttttcaaga tacccggatc atatgaaacg gcatgacttt
     4501 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
     4561 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     4621 gagttaaaag gtattgattt taaagaagat ggaaacattc ttgggcacaa attggaatac
     4681 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagct
     4741 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
     4801 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
     4861 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     4921 gtaacagctg ctgggattac acatggcatg gatgaactat acaaaggatc cggctgctaa
     4981 caaagcccga aaggacggtc tatagaggtg tgttaaaagc agacttttga acatccacag
     5041 aaattggctc aatgacctat ccgaaaagaa tacctataaa tgcgtggtct gaagttcata
     5101 gggtggcaag gccactaatt attactttcg acgcatataa tacactatac gccacaaaac
     5161 ttcccgtcat ggaacaatac tgcattgttg ggcgtaaata tggtatcaag gcgaatcctt
     5221 cgactttaac aaacaatttt ccacacgttt ttaaaaaact gaaggaagac tatccccaat
     5281 acggaaaata ttctggtatc aagcccgaac aatggtggtc catcttgatt aggaacgtct
     5341 tcgcaccaaa cgaaattccc gatgagatga taaatgaaat attaatgcgg tttgagggct
     5401 tcgattcgta ttttgtttat ccagatttga tcaaattttt aaaggatttg aagtcaagac
     5461 atcctgatgt tatcttagga atagtaagca atactgatcc aattttttac aaattattaa
     5521 agaatatcgg attatttgag acattttccg gtcacattta tttatcatat gagcttaatc
     5581 tcgccaaacc tgatagagca atatttcaat atgcgcttga cgatattata tctaaacaac
     5641 ctcatttact cgaaaagtac accagggagg aaattctaca gcattgcttt cacatcggag
     5701 atgaattaaa gaacgatctc gaaggggcag aagctgccgg ttggactggt atactgttag
     5761 atagaaatga taaatacggg tttctttcaa actcgattag taaaccaatg cgggacgaat
     5821 acaagctgtc aattgataaa atagataata attcaataaa cacttgggaa gccaatacta
     5881 agcaaactga cacgttacag ttgagtgaaa gaaagtacgt tgtttcgaat cttgaggttt
     5941 tagaggaact ctttccctga gcgcctaacg ttacagtaac aaatatgtat gatagatatt
     6001 attcatatcc gccagtaata tttactatat ctttgaatgg gtttgtttat taataatcta
     6061 ctatgtgtaa atatatgatc caagtattat acagtttaga cactgattgt tttccagacg
     6121 ttcaaaccgt cagtaccggt acttactaaa caacctggga tttgcttatg ccatttgaca
     6181 tctttaactt ccttttgcca gtgaacaaac aataattgtg gtggaatttc ttgtagctct
     6241 tttgtttcgg cggcctgttg tttaatttcc tcatcgtcag cttctacaga caaatcccac
     6301 aaagtcacag tattatcttc tgagcctacc gcaacgatag actcatctaa tgggttgaat
     6361 gcaatggaag taatggctcc cttatggaag tcatattgag caaccggttg gacggcgtca
     6421 gcattacttg gcgtaaactg tcttaaatcc catactcccc atgtaccgtt atcgtcaccg
     6481 cttgctagca agtaaccaat tttatcactc caacttatga catttacgtc agtattagaa
     6541 gctttaacag agatagcagg tttatgtttt tttgatcttg tgtcccaaat ccttatatat
     6601 ccatcacatc ctgcggttgc aaaaacggtg gattcagtgc gagaccactg gatatcttct
     6661 atggatttat tgtttgaaac agtaaatggt tgtttatcag tcacccatct cgatgtgtgc
     6721 ctttgggtaa aatatatttg tcctgagcaa tcacctgata gtaacgcacc agtcttgatc
     6781 aatggtgacc aatccaaccc gtagccttca acattcccat gattttttac agtgtgaata
     6841 ggacgcttag cagacttcgg aatctgataa cctggtgtgg aaaaagcttt gctttgtgga
     6901 gctagattgt atatataaac atcaccattt tcgctcattg tagcggttaa cacctcttga
     6961 ttagaaatgg caaaaggaga aacctttaat ctattggttg tatctctcaa tggtatattt
     7021 tcattctcaa tgactggatc cactagttct agagcggccg ccaccgcggt ggagctccaa
     7081 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     7141 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7201 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7261 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7321 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7381 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7441 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7501 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7561 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7621 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7681 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     7741 tccttacgca tctgtgcggt atttcacacc gcatatcgac cggtcgagga gaacttctag
     7801 tatatctaca tacctaatat tattgcctta ttaaaaatgg aatcccaaca attacatcaa
     7861 aatccacatt ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg tgttcaaaaa
     7921 cgttatattt ataggataat tatactctat ttctcaacaa gtaattggtt gtttggccga
     7981 gcggtctaag gcgcctgatt caagaaatat cttgaccgca gttaactgtg ggaatactca
     8041 ggtatcgtaa gatgcaagag ttcgaatctc ttagcaacca ttattttttt cctcaacata
     8101 acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt
     8161 taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg gagaaaaagg
     8221 aaaggtgaga gcgccggaac cggcttttca tatagaatag agaagcgttc atgactaaat
     8281 gcttgcatca caatacttga agttgacaat attatttaag gacctattgt tttttccaat
     8341 aggtggttag caatcgtctt actttctaac ttttcttacc ttttacattt cagcaatata
     8401 tatatatata tttcaaggat ataccattct aatgtctgcc cctaagaaga tcgtcgtttt
     8461 gccaggtgac cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat
     8521 ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc
     8581 tatcgatgct acaggtgttc cacttccaga tgaggcgctg gaagcctcca agaaggctga
     8641 tgccgttttg ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga
     8701 acaaggttta ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa
     8761 ctttgcatcc gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac
     8821 tgacttcgtt gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga
     8881 cgatggtgat ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat
     8941 cacaagaatg gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt
     9001 ggataaagct aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat
     9061 caagaacgaa ttccctacat tgaaggttca acatcaattg attgattctg ccgccatgat
     9121 cctagttaag aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga
     9181 tatcatctcc gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc
     9241 cttggcctct ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc
     9301 tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc actatcttgt ctgctgcaat
     9361 gatgttgaaa ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa
     9421 aaaggttttg gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga
     9481 agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt
     9541 tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt
     9601 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat
     9661 caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct
     9721 caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga ggagggcacc
     9781 acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt tatatattgg
     9841 aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat
     9901 ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc
     9961 aagaatttta ctctgtcaga aacggcctta acgacgtagt cgatatggtg cactctcagt
    10021 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
    10081 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
    10141 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
    10201 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttaggacgga
    10261 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
    10321 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
    10381 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
    10441 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
    10501 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
    10561 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
    10621 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt
    10681 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat
    10741 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg
    10801 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10861 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10921 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10981 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    11041 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    11101 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    11161 agagcaactc ggtcgccgca tacac
//
