LOCUS       sequence-368109-       12665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12665)
  AUTHORS   Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
  TITLE     Conditional control of fluorescent protein degradation by an 
            auxin-dependent nanobody.
  JOURNAL   Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.
  PUBMED    30120238
REFERENCE   2  (bases 1 to 12665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-17"; volume: "9(1):3297. doi"; pages: " 
            10.1038/s41467-018-05855-5"
COMMENT     Sequence Label: pCAGGs-NLS-TIR1_P2A_NES-TIR1
FEATURES             Location/Qualifiers
     source          1..12665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    71..92
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(107..112,113..130,131..137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    145..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     150..172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     169..185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     169..185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    232..456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     445..462
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(543..1142)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(626..646)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1123..1142
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(1143..1151,1152..1154,1155..1729)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1316..1335)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1545..1562
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(1756..3480)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             complement(3484..3510)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(3520..3549)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3559..3615)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3625..3651)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3655..5376)
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             complement(5383..5403)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5415..5424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          5485..6501
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5539..5561)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(6502..6778)
                     /label=chicken beta-actin promoter
     enhancer        6780..7159
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    7173..7307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(7900..7968,7969..8760)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8931..9519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    9758..9892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(10485..10553,10554..11345)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11516..12103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    12342..12476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(join(12482..12665,1..12))
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12496..12631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 taattgagat gcggatccgc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
       61 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      121 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      181 atgaccatga ttacgccaag cttgggctgc aggttctttc cgcctcagaa gccatagagc
      241 ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
      301 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
      361 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
      421 ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag
      481 atcatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
      541 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
      601 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
      661 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
      721 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
      781 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
      841 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
      901 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
      961 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     1021 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     1081 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     1141 atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg
     1201 ggggcctaga cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc
     1261 cagatcagat cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac
     1321 gcttgaggag agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg
     1381 ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt tggccgcaga ggcacctgtc
     1441 gccaggtggg gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga
     1501 agcttccaga ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag
     1561 aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac
     1621 attgccaaaa gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta
     1681 ttccaagcgg cttcggccag taacgttagg ggggggggag ggagaggggg gggggncgga
     1741 attcttaatt aactatcaca gtattttcac gaagtttgga gcgtcatccc ttgccccagc
     1801 agtggttctg tagacataca acttttccac cttgggaaga tctccgtggt tctcttccat
     1861 ttcattggat ccatctctct cgttgatcac ctccacattc agcattggca tttttgaggc
     1921 cagcacttga catcccttga gtgtaacatt acagctggac atccaaagag acctcatagt
     1981 ttcgtatctt gcgaagttac caaggagggc tgcatctccg aatggactgt ctcttatctc
     2041 aagctttctc aagttcttac atccattcat aacatgcatc atccccttgt cagaatcacc
     2101 ggcaaaggca atagacagca tttccagctg cttagcgtac ttaccaatgt acataaacac
     2161 cttgtcagtc aggagtccac tgatagagag tctttggagt cccttacatt ctctcacaat
     2221 cgccccaaat ccttcgtcca agggctgact ggtgaccacg tcaggtttcc caggttcaag
     2281 gatacaaagt ctgaagcgtg tgaagttagg gcaatttttg gccactgtca caagggcagc
     2341 gttagtcatt tggtgacaaa agtacaagag tgaattcaac ttgggacatc caagggagac
     2401 agcaaccaac ccttcctcag ttacagcact gtaacctgcc acatagaagt cacttggaaa
     2461 cactctcaac tcttggagat ccttacaaga tgatgccacc acttgaagtc ctttgtcgga
     2521 gatacaatcg aggacccaaa gtctttgaag cttcacgcat cttgaaatca tcttggtcaa
     2581 gtcactcgca tccagtgttg gagcatagga caagttgagt ccagtcagct gggcacaaag
     2641 agggtagatg aaggaaagac aaacaggtga agcatcccag aatccggaca gtgatctcaa
     2701 catcttacac ttttccaaag cactagtcaa cttaaagtaa gactcagttt ggaagtcatc
     2761 cgtgagattc ccagttccaa ggtcctccaa attgggggtt ctcaagagaa tctttgccaa
     2821 ggtatccaca ctcacagatc tgttgagcct cagtgatcgc aagttgggac ttcttgagac
     2881 caatctctca agactcccgg cattgacctc tcctttgata cacgcgaaat tcaaggaaac
     2941 aagggaagta catgagtcag ggaaacatga caaccatctt ggtcctctgt cctcaacctc
     3001 attttcttgg aggtccaact ccctcaacaa cttacagtgg gaagcgactg cagcgagtcc
     3061 gtcagtagag aaaccctcac aagaaatcaa gaccaaagct ctgaatctgg ggaaggaccg
     3121 ggccagaagt tcaagggact catcagacac aaccatcctc ttcatcctca actcctccaa
     3181 cccatgacaa cctctggcag cagcctcaat ccatggtcca gcataacctc cccagtccgg
     3241 aggaaccaaa ttgaaatcag caaaatgtgg tttaccctta actgtaagtg ccctcacatt
     3301 ggggaatctg gcagcgactc ttccagccct taccgcatag cagtttccca caaacactcc
     3361 tctccttgac aacctttcaa tctcatacca aaccttacat accaaagaga ctgtattccg
     3421 gtctctttgg gcaggaagaa aagaaaagat atgttccaca acttcttcag ggaagtacgt
     3481 ccctagcgta agtctttcta gcgggggaag ctgaaggggt aagtcttctt cgcttattaa
     3541 cttctgttcc ttaattaaag gtccagggtt ctcctccacg tctccagcct gcttcagcag
     3601 gctgaagtta gtagctccgc ttcccgcata gtcaggaaca tcgtatgggt agcccagtat
     3661 tttcacgaag tttggagcgt catcccttgc cccagcagtg gttctgtaga catacaactt
     3721 ttccaccttg ggaagatctc cgtggttctc ttccatttca ttggatccat ctctctcgtt
     3781 gatcacctcc acattcagca ttggcatttt tgaggccagc acttgacatc ccttgagtgt
     3841 aacattacag ctggacatcc aaagagacct catagtttcg tatcttgcga agttaccaag
     3901 gagggctgca tctccgaatg gactgtctct tatctcaagc tttctcaagt tcttacatcc
     3961 attcataaca tgcatcatcc ccttgtcaga atcaccggca aaggcaatag acagcatttc
     4021 cagctgctta gcgtacttac caatgtacat aaacaccttg tcagtcagga gtccactgat
     4081 agagagtctt tggagtccct tacattctct cacaatcgcc ccaaatcctt cgtccaaggg
     4141 ctgactggtg accacgtcag gtttcccagg ttcaaggata caaagtctga agcgtgtgaa
     4201 gttagggcaa tttttggcca ctgtcacaag ggcagcgtta gtcatttggt gacaaaagta
     4261 caagagtgaa ttcaacttgg gacatccaag ggagacagca accaaccctt cctcagttac
     4321 agcactgtaa cctgccacat agaagtcact tggaaacact ctcaactctt ggagatcctt
     4381 acaagatgat gccaccactt gaagtccttt gtcggagata caatcgagga cccaaagtct
     4441 ttgaagcttc acgcatcttg aaatcatctt ggtcaagtca ctcgcatcca gtgttggagc
     4501 ataggacaag ttgagtccag tcagctgggc acaaagaggg tagatgaagg aaagacaaac
     4561 aggtgaagca tcccagaatc cggacagtga tctcaacatc ttacactttt ccaaagcact
     4621 agtcaactta aagtaagact cagtttggaa gtcatccgtg agattcccag ttccaaggtc
     4681 ctccaaattg ggggttctca agagaatctt tgccaaggta tccacactca cagatctgtt
     4741 gagcctcagt gatcgcaagt tgggacttct tgagaccaat ctctcaagac tcccggcatt
     4801 gacctctcct ttgatacacg cgaaattcaa ggaaacaagg gaagtacatg agtcagggaa
     4861 acatgacaac catcttggtc ctctgtcctc aacctcattt tcttggaggt ccaactccct
     4921 caacaactta cagtgggaag cgactgcagc gagtccgtca gtagagaaac cctcacaaga
     4981 aatcaagacc aaagctctga atctggggaa ggaccgggcc agaagttcaa gggactcatc
     5041 agacacaacc atcctcttca tcctcaactc ctccaaccca tgacaacctc tggcagcagc
     5101 ctcaatccat ggtccagcat aacctcccca gtccggagga accaaattga aatcagcaaa
     5161 atgtggttta cccttaactg taagtgccct cacattgggg aatctggcag cgactcttcc
     5221 agcccttacc gcatagcagt ttcccacaaa cactcctctc cttgacaacc tttcaatctc
     5281 ataccaaacc ttacatacca aagagactgt attccggtct ctttgggcag gaagaaaaga
     5341 aaagatatgt tccacaactt cttcagggaa gtacgtgtcc agcacctttc tcttcttctt
     5401 gggggcggcg gggcccatgg tggcggcggg taattctttg ccaaaatgat gagacagcac
     5461 aacaaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc
     5521 agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc cccgtccccc
     5581 ccgaaggcag ccgtcccccc gcggacagcc ccgaggctgg agagggagaa ggggacggcg
     5641 gcgcggcgac gcacgaaggc cctccccgcc catttccttc ctgccggcgc cgcaccgctt
     5701 cgccccgcgc ccgctagagg gggtgcggcg gcgcctccca gatttcggct ccgcacagat
     5761 ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc aatggctgcg
     5821 gctcgccgcg cctcgacagc cgccggcgct ccgggggccg ccgcgcccct cccccgagcc
     5881 cyccccggcc cgaggcggcc ccgccccgcc cggcaccccc acctgccgcc accccccgcc
     5941 cggcacggcg agccccgcgc cacgccccgt acggagcccc gcacccgaag ccgggccgtg
     6001 ctcagcaact cggggagggg ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc
     6061 ccctgctcac ccccccacgc acacaccccg cacgcagcct ttgttcccct cgcagccccc
     6121 cccgcaccgc ggggcaccgc ccccggccgc gctcccctcg cgcacactgc ggagcgcaca
     6181 aagccccgcg ccgcgcccgc agcgctcaca gccgccgggc agcgcggagc cgcacgcggc
     6241 gctccccacg cacacacaca cgcacgcacc ccccgagccg ctccccccgc acaaagggcc
     6301 ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac
     6361 caagcgctaa ttacagcccg gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa
     6421 cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg
     6481 aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg
     6541 ccgccgcctc gccataaaag gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc
     6601 acctctccgc cycsccccsc cccsccccyc sccccscccc sccccsccyg gcgcgcgccc
     6661 cccccccccc ccgcccccat cgctgcacaa aataattaaa aaataaataa atacaaaatt
     6721 gggggtgggg agggggggga gatggggaga gtgaagcaga acgtggggct cacctcgacc
     6781 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     6841 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     6901 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     6961 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     7021 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     7081 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     7141 ctagtcaata atcaatgtcg acgatgatat cagatccaga catgataaga tacattgatg
     7201 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     7261 atgctattgc tttatttgta accattataa gctgcaataa acaagttaat tcgattcagc
     7321 ttcagaagat ggcggagggc ctccaacaca gtaattttcc tcccgactct taaaatagaa
     7381 aatgtcaagt cagttaagca ggaagtgact aactgaccgc agctggccgt gcgacatcct
     7441 cttttaatta gttgctaggc aactgccctc cagagggcag tgtggttttg caagaggaag
     7501 caaaaagcct ctccacccag gcctggaatg tcagtgtggt tttgcaagag gaagcaaaaa
     7561 gcctctccac ccaggcctgg aatgtttcca cccaatcatt actatgacaa cagctgtttt
     7621 ttttagtatt aagcagaggc cgggggcccc tggcctccgc ttactctgga gaaaaagaag
     7681 agaggcttcc agaggcaact tgtcaaaaca ggactggcgc cttggaggcg ctgtggggcc
     7741 acccaaattg atataattaa gccccaaccg cctcttcccg cctcatttca gcctcaccac
     7801 tatgtgaata tagctgaata cacagtttat tgaataaaca ttaatttcca ggaaatacag
     7861 tctttgtttt tccaaagcgg tcattgaaaa aggaagagta tgagtattca acatttccgt
     7921 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7981 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8041 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8101 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8161 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8221 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8281 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8341 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8401 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8461 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8521 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8581 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8641 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8701 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8761 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8821 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8881 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8941 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9001 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9061 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9721 cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga taagatacat
     9781 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     9841 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaattcgat
     9901 tcagcttcag aagatggcgg agggcctcca acacagtaat tttcctcccg actcttaaaa
     9961 tagaaaatgt caagtcagtt aagcaggaag tgactaactg accgcagctg gccgtgcgac
    10021 atcctctttt aattagttgc taggcaactg ccctccagag ggcagtgtgg ttttgcaaga
    10081 ggaagcaaaa agcctctcca cccaggcctg gaatgtcagt gtggttttgc aagaggaagc
    10141 aaaaagcctc tccacccagg cctggaatgt ttccacccaa tcattactat gacaacagct
    10201 gtttttttta gtattaagca gaggccgggg gcccctggcc tccgcttact ctggagaaaa
    10261 agaagagagg cttccagagg caacttgtca aaacaggact ggcgccttgg aggcgctgtg
    10321 gggccaccca aattgatata attaagcccc aaccgcctct tcccgcctca tttcagcctc
    10381 accactatgt gaatatagct gaatacacag tttattgaat aaacattaat ttccaggaaa
    10441 tacagtcttt gtttttccaa agcggtcatt gaaaaaggaa gagtatgagt attcaacatt
    10501 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10561 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10621 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10681 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    10741 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10801 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10861 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10921 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10981 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11041 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11101 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11161 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11221 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11281 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11341 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11401 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11521 atcctttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    11581 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    11641 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    11701 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    11761 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11821 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    11881 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    11941 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    12001 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    12061 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    12121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    12181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    12241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    12301 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc ggatccagac atgataagat
    12361 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
    12421 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttagct
    12481 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
    12541 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
    12601 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
    12661 ctgac
//
