LOCUS       sequence-368110-       11185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1st 200 amino acids (N-term + transmembrane domain) of Pom152 fused 
            to HA and GFP with S158A, F159A, F160A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Brown JT, Haraczy AJ, Wilhelm CM, Belanger KD
  TITLE     Characterization of nuclear pore complex targeting domains in Pom152
            in Saccharomyces cerevisiae.
  JOURNAL   Biol Open. 2021 Oct 15;10(10). pii: 272274. doi: 10.1242/bio.057661.
            Epub 2021 Oct 20.
  PUBMED    34557894
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2021-10-15"; volume: "10(10). pii: 272274. doi"; 
            pages: " 10.1242/bio.057661. Epub 2021 Oct 20"
COMMENT     Sequence Label: KBB556
FEATURES             Location/Qualifiers
     source          1..11185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        224..242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     270..286
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     271..287
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2718..3428
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2745..2773)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3368..3388
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(5500..5516)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5500..5516)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5548..5567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5549..5567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5574..5591)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5574..5590)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5583..5605)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5731..6186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5818..5837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6028..6049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6487..6894
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6895..7989
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(8483..8502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8602..8624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8662..8680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8722..9225
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        9262..9366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9367..9435,9436..10227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9585..9604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10398..10986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10887..10906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11140..11157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac cctcactaaa
      241 gggaacaaaa gctggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
      301 cgaattcctg cagcccgggg gatccacagt tgacaacgat ctcaagattg gttgggattt
      361 aaatcctata gcagtgcatc agaagaagca aaaaggccag tggacagtca ttaagtttat
      421 caccagaaag ggcttccagc tgagagatcc cctcataatt tccccaaaac gtaaccatgt
      481 gtgaatagat tttgagtaag cagggttgca gccacgagtg agtcttccct tgttattgtg
      541 tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt
      601 ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca
      661 agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa acgctctgag
      721 ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa gtctcggaag
      781 gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg tgttcgaatt
      841 tcaacaggta agttagttgc tagaatccat ggctcctttg ccgacgctga gtagatttta
      901 ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc tttagagcga
      961 gatttataca atagaatttg gcatgagatt gggttgcttt tagtcagcct cttatagcct
     1021 aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt ccagttttcc
     1081 gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta ccaacccagc
     1141 ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat gagcggtgtg
     1201 gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc tttctttgaa
     1261 cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc gtgtagtgcg
     1321 actgaagctc gcaataccat aagccgtttt tgctcacgga tatcagacca gtcaacaagt
     1381 acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta tacagcgagt
     1441 cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat gttatgtttt
     1501 gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt tttacagtca
     1561 agtaaggcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa ttcggggcag
     1621 aattggtaaa gagagtcgtg taaaatatcg agttcgcaca ttttgttgtc tgattattga
     1681 tttttggcga aaccatttga tcatatgaca agatgtgtat ctaccttaac ttaatgattt
     1741 tgataaaaat cattagggga ttcatcaggg cttccagacc atcacaggtg aagagaaaga
     1801 aaaaaaataa tgcgctggta ttatagaaat agagtttcat tatataagaa catataggaa
     1861 gaatacatta ttcctatcta gggcatcaaa taattgcaac cacttaatgt taacttcctt
     1921 cataattcaa aacattatgt tctatagcac tatgtatttt atgtcattat atacgtagtc
     1981 aataacttta tacgtttttt ttattgaaca aaagtaagaa ttgcttaaga cataataaga
     2041 ggaaactcat cgaaccaatt caaaacggat tatagattta tcataccaga tacgtttatc
     2101 aggaggcttg tataatggag cacagatata acgtgtttaa tgatactcca cgagggaacc
     2161 attggatggg cagttctgtg tcaggatcac cacgtccatc ttatagcagt cgtcctaatg
     2221 taaacacaac acgacgcttc caatatagtg acgatgagcc cgctgagaaa atccgacctt
     2281 tacgctcgag gagctttaaa agtactgaaa gtaacataag cgatgaaaaa tcaaggatat
     2341 ctgaacgtga cagcaaagac cgctacatta atggtgataa aaaggtagac atttattcac
     2401 tgcctctaat atcaaccgat gttttagaaa tttccaaaca aaggacattt gcggtgatat
     2461 tgtttttaat aattcaatgt tataaaatat atgacctggt aatacttaaa tcggggttac
     2521 cgctttcggg tttactgttt aagaactatc gatttaattt catatccaag tatttcatta
     2581 tagatgccgc cgccctgtat gttttaccat cctttaacat tcctaggttg acttttaagc
     2641 catgggttgt ttatctccag attttagcta tgctactatt gaacattttc atatcaagcg
     2701 atcacgagtt cgttgctagc aaaggagaag aactcttcac tggagttgtc ccaattcttg
     2761 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     2821 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2881 catggccaac acttgtcact actttcactt atggtgttca gtgcttttca agatacccgg
     2941 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     3001 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     3061 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     3121 ttcttgggca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     3181 aacaaaagaa tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaagcg
     3241 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     3301 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     3361 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     3421 tatacaaagg atccggctgc taacaaagcc cgaaaggacg gtctatagag gtgtgttaaa
     3481 agcagacttt tgaacatcca cagaaattgg ctcaatgacc tatccgaaaa gaatacctat
     3541 aaatgcgtgg tctgaagttc atagggtggc aaggccacta attattactt tcgacgcata
     3601 taatacacta tacgccacaa aacttcccgt catggaacaa tactgcattg ttgggcgtaa
     3661 atatggtatc aaggcgaatc cttcgacttt aacaaacaat tttccacacg tttttaaaaa
     3721 actgaaggaa gactatcccc aatacggaaa atattctggt atcaagcccg aacaatggtg
     3781 gtccatcttg attaggaacg tcttcgcacc aaacgaaatt cccgatgaga tgataaatga
     3841 aatattaatg cggtttgagg gcttcgattc gtattttgtt tatccagatt tgatcaaatt
     3901 tttaaaggat ttgaagtcaa gacatcctga tgttatctta ggaatagtaa gcaatactga
     3961 tccaattttt tacaaattat taaagaatat cggattattt gagacatttt ccggtcacat
     4021 ttatttatca tatgagctta atctcgccaa acctgataga gcaatatttc aatatgcgct
     4081 tgacgatatt atatctaaac aacctcattt actcgaaaag tacaccaggg aggaaattct
     4141 acagcattgc tttcacatcg gagatgaatt aaagaacgat ctcgaagggg cagaagctgc
     4201 cggttggact ggtatactgt tagatagaaa tgataaatac gggtttcttt caaactcgat
     4261 tagtaaacca atgcgggacg aatacaagct gtcaattgat aaaatagata ataattcaat
     4321 aaacacttgg gaagccaata ctaagcaaac tgacacgtta cagttgagtg aaagaaagta
     4381 cgttgtttcg aatcttgagg ttttagagga actctttccc tgagcgccta acgttacagt
     4441 aacaaatatg tatgatagat attattcata tccgccagta atatttacta tatctttgaa
     4501 tgggtttgtt tattaataat ctactatgtg taaatatatg atccaagtat tatacagttt
     4561 agacactgat tgttttccag acgttcaaac cgtcagtacc ggtacttact aaacaacctg
     4621 ggatttgctt atgccatttg acatctttaa cttccttttg ccagtgaaca aacaataatt
     4681 gtggtggaat ttcttgtagc tcttttgttt cggcggcctg ttgtttaatt tcctcatcgt
     4741 cagcttctac agacaaatcc cacaaagtca cagtattatc ttctgagcct accgcaacga
     4801 tagactcatc taatgggttg aatgcaatgg aagtaatggc tcccttatgg aagtcatatt
     4861 gagcaaccgg ttggacggcg tcagcattac ttggcgtaaa ctgtcttaaa tcccatactc
     4921 cccatgtacc gttatcgtca ccgcttgcta gcaagtaacc aattttatca ctccaactta
     4981 tgacatttac gtcagtatta gaagctttaa cagagatagc aggtttatgt ttttttgatc
     5041 ttgtgtccca aatccttata tatccatcac atcctgcggt tgcaaaaacg gtggattcag
     5101 tgcgagacca ctggatatct tctatggatt tattgtttga aacagtaaat ggttgtttat
     5161 cagtcaccca tctcgatgtg tgcctttggg taaaatatat ttgtcctgag caatcacctg
     5221 atagtaacgc accagtcttg atcaatggtg accaatccaa cccgtagcct tcaacattcc
     5281 catgattttt tacagtgtga ataggacgct tagcagactt cggaatctga taacctggtg
     5341 tggaaaaagc tttgctttgt ggagctagat tgtatatata aacatcacca ttttcgctca
     5401 ttgtagcggt taacacctct tgattagaaa tggcaaaagg agaaaccttt aatctattgg
     5461 ttgtatctct caatggtata ttttcattct caatgactgg atccactagt tctagagcgg
     5521 ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     5581 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5641 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5701 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     5761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5941 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6121 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     6181 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatc
     6241 gaccggtcga ggagaacttc tagtatatct acatacctaa tattattgcc ttattaaaaa
     6301 tggaatccca acaattacat caaaatccac attctcttca aaatcaattg tcctgtactt
     6361 ccttgttcat gtgtgttcaa aaacgttata tttataggat aattatactc tatttctcaa
     6421 caagtaattg gttgtttggc cgagcggtct aaggcgcctg attcaagaaa tatcttgacc
     6481 gcagttaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa
     6541 ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca cagaatcaaa
     6601 ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat acctttttca
     6661 actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt tcatatagaa
     6721 tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac aatattattt
     6781 aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct aacttttctt
     6841 accttttaca tttcagcaat atatatatat atatttcaag gatataccat tctaatgtct
     6901 gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa
     6961 gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa
     7021 aatcatttaa ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg
     7081 ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg
     7141 ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg
     7201 tacgccaact taagaccatg taactttgca tccgactctc ttttagactt atctccaatc
     7261 aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt
     7321 tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac
     7381 accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag
     7441 ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg
     7501 agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt tcaacatcaa
     7561 ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata
     7621 atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggttcc
     7681 ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt
     7741 ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt caaccctatc
     7801 gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt
     7861 aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta
     7921 ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc
     7981 cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat aaatgaaatt
     8041 cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat
     8101 atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg
     8161 taagcaaatt gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta
     8221 atattgacaa ggaggagggc accacacaaa aagttaggtg taacagaaaa tcatgaaact
     8281 atgattccta atttatatat tggaggattt tctctaaaaa aaaaaaaata caacaaataa
     8341 aaaacactca atgacctgac catttgatgg agtttaagtc aataccttct tgaaccattt
     8401 cccataatgg tgaaagttcc ctcaagaatt ttactctgtc agaaacggcc ttaacgacgt
     8461 agtcgatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     8521 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8581 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8641 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8701 taataatggt ttcttaggac ggatcgcttg cctgtaactt acacgcgcct cgtatctttt
     8761 aatgatggaa taatttggga atttactctg tgtttattta tttttatgtt ttgtatttgg
     8821 attttagaaa gtaaataaag aaggtagaag agttacggaa tgaagaaaaa aaaataaaca
     8881 aaggtttaaa aaatttcaac aaaaagcgta ctttacatat atatttatta gacaagaaaa
     8941 gcagattaaa tagatataca ttcgattaac gataagtaaa atgtaaaatc acaggatttt
     9001 cgtgtgtggt cttctacaca gacaagatga aacaattcgg cattaatacc tgagagcagg
     9061 aagagcaaga taaaaggtag tatttgttgg cgatccccct agagtctttt acatcttcgg
     9121 aaaacaaaaa ctattttttc tttaatttct ttttttactt tctattttta atttatatat
     9181 ttatattaaa aaatttaaat tataattatt tttatagcac gtgatgaaaa ggacccaggt
     9241 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9301 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9361 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9421 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9481 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9541 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9601 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     9661 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     9721 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     9781 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     9841 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     9901 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     9961 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10021 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10081 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10141 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10201 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    10681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    10801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    10861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    10921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    10981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11161 gcggaagagc gcccaatacg caaac
//
