LOCUS       sequence-368112-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3CG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3CG
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     86..105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        86..104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    122..221
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(614..625)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca cgggccagag
       61 ctgccaggaa acagctatga ccatgtaata cgactcacta taggggatat cagctggatg
      121 gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca
      181 atgctttctt ataatgccaa ctttgtacaa gaaagctggg ttctaggctg aatgtttctc
      241 tccttgtttg atgccaagaa caagatgtag aaaccaatta aactgcacag tccatccttt
      301 gtctctgcaa acttcgatct gatcaagaaa atacttttta gcatcctcct catttttccc
      361 cactgtgagg gcatcccgga tatattcaat gtcttctttg cttgttaact ggggcattcc
      421 tgtcatcagc atcatggaga acaggatgat cagtaggttt gtgtgatgac gaagggctag
      481 ataagcctta acacagatgt cctgaaattt ctggaagtgt gggcttgtct tctttccaga
      541 agttcccatc acaaagagga agtcaggggt tagcacaaat ggcactctct ctttattaat
      601 gcccaggaaa cttttgtaat tcccaagaat gtgcccgaag tcaatatgaa ataggtttcc
      661 tgtctcggtg atcataatat tgtcattgtg tctgtcgcct attccaagaa caaaggttgc
      721 cacacagtag cctgcacagg aataaacaaa tctctccact gctgcctgaa acttttcttc
      781 agtaggggat ttttctttga gccagtgatt caggacttca tctttaaatg ctcccgtgtt
      841 gcccactgtg ctttgctgaa ttttggcaat tgtcgtggcg tct
//
