LOCUS       sequence-368115-       13686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HA-tagged POM152 with glycosylation sites mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13686)
  AUTHORS   Belanger KD, Gupta A, MacDonald KM, Ott CM, Hodge CA, Cole CM, Davis
            LI
  TITLE     Nuclear pore complex function in Saccharomyces cerevisiae is 
            influenced by glycosylation of the transmembrane nucleoporin 
            Pom152p.
  JOURNAL   Genetics. 2005 Nov;171(3):935-47. doi: 10.1534/genetics.104.036319. 
            Epub 2005 Aug 22.
  PUBMED    16118201
REFERENCE   2  (bases 1 to 13686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.104.036319"; journalName: "Genetics"; date: 
            "2005-11"; volume: "171"; issue: "3"; pages: "935-47"
COMMENT     Sequence Label: KBB382
FEATURES             Location/Qualifiers
     source          1..13686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        447..551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(552..620,621..1412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1583..2171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2072..2091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2325..2342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2459..2480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2495..2500,2501..2518,2519..2525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2533..2549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2538..2560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2557..2573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2557..2573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2594..2612
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2640..2656
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2641..2657
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4035..4061
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4077..4103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4119..4145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9573..9589)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9573..9589)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9621..9640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9622..9640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9647..9664)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9647..9663)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9656..9678)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9804..10259
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9891..9910)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10101..10122
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10560..10967
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             10968..12062
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     12675..12697
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12735..12753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    12795..13298
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        13335..13439
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
       61 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
      121 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
      181 gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct
      241 tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc gcgcactact
      301 cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg gcatcagagc
      361 agattgtwmw garaktbvam yhwwrrkkat ykkkakakcw csygatsmra nnngnaccnn
      421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2401 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2581 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct
     2641 cgaggtcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccacagt
     2701 tgacaacgat ctcaagattg gttgggattt aaatcctata gcagtgcatc agaagaagca
     2761 aaaaggccag tggacagtca ttaagtttat caccagaaag ggcttccaga ccatcacagg
     2821 tgaagagaaa gaaaaaaaat aatgcgctgg tattatagaa atagagtttc attatataag
     2881 aacatatagg aagaatacat tattcctatc tagggcatca aataattgca accacttaat
     2941 gttaacttcc ttcataattc aaaacattat gttctatagc actatgtatt ttatgtcatt
     3001 atatacgtag tcaataactt tatacgtttt ttttattgaa caaaagtaag aattgcttaa
     3061 gacataataa gaggaaactc atcgaaccaa ttcaaaacgg attatagatt tatcatacca
     3121 gatacgttta tcaggaggct tgtataatgg agcacagata taacgtgttt aatgatactc
     3181 cacgagggaa ccattggatg ggcagttctg tgtcaggatc accacgtcca tcttatagca
     3241 gtcgtcctaa tgtaaacaca acacgacgct tccaatatag tgacgatgag cccgctgaga
     3301 aaatccgacc tttacgctcg aggagcttta aaagtactga aagtaacata agcgatgaaa
     3361 aatcaaggat atctgaacgt gacagcaaag accgctacat taatggtgat aaaaaggtag
     3421 acatttattc actgcctcta atatcaaccg atgttttaga aatttccaaa caaaggacat
     3481 ttgcggtgat attgttttta ataattcaat gttataaaat atatgacctg gtaatactta
     3541 aatcggggtt accgctttcg ggtttactgt ttaagaacta tcgatttaat ttcatatcca
     3601 agtatttcat tatagattcc ttcttcctgt atgttttacc atcctttaac attcctaggt
     3661 tgacttttaa gccatgggtt gtttatctcc agattttagc tatgctacta ttgaacattt
     3721 tcatatcaag cgatcacgag ttcgttttga tttcattaat tatgaccaca tggagaaaac
     3781 tttatacgaa agaattaagt gtaacaggtt cagccattaa ccatcatcgt atttttgatt
     3841 cttccgctca ttttaagggt gctctaacaa tcaagatcct acctgaaaac acagctatgt
     3901 ttaatcctct tcatgaatca tattgtttgc ccatggatac caatcttttt aaaattaact
     3961 caattgacgt acccatcaga atagcttcaa ctgaagagat tgaatatatc gagttggaat
     4021 atagagatct tggttaccca tacgacgtcc cagattacgc tagcggtgat cttggttacc
     4081 catacgacgt cccagattac gctagcggtg atcttggtta cccatacgac gtcccagatt
     4141 acgctagcgg tgatctttac acaaattcgg tagagttacg gtctttgagt aagaaagact
     4201 tcaaaatcat cgataacccc aaatcctttt taaaaaaaga tcagtcagtt ctaaagtcac
     4261 attcaaacga ttttgaagaa ggcagtacaa ttcgttattt ggctgtaact cttcaagata
     4321 ttggctttta tcaaatcaaa aaaattgttg attcaaaaaa actgaattta aagatccatc
     4381 agtcacactt ggttgtaccc tactgtccaa ttgcatctat tactggaact ggaagcaatg
     4441 atagatgtat tggcgattca gatgctgttt cttttgaaat tcaaggtgtg cccccaatga
     4501 aattagcata ctctaagatc gttaatggtc aaactttttc atatgtagac tccagcttgc
     4561 aaccagaata ttttgagtct cctttgcagt caagcaaatc caaacaaagt tttactcaag
     4621 gagaactaaa cgatttgaaa tggggaagaa accaacctgt taatattaat ttagactctt
     4681 ccattaccca ggacggcaaa tttgcatata aaattgataa aataactgat ggtctgggaa
     4741 atgtcgtgga ttttacatct ctccctgaag aattaaaaaa gcgctatgat ctatcttata
     4801 atttcaatgt tcatgaggtt ccacgtgcag cgctagaaga aagatttgat ccaaagtcac
     4861 ccacaaaaag gtctattgcc atcgtgtttg aagaaatcaa aaactggatt tcagacattc
     4921 catatgttat tagcctatcc tatactgatg cacaagacaa gtcgaaaaag ataatggctg
     4981 ttactacaga ttctttgacc aaagtgttgc aagcggatct tccggggtca tataacttag
     5041 aatatattga atctaagttt tgtccaggtg aaattgtggg taaatcaaac gttcttgtaa
     5101 ctatgccagt tgcacctact atggaagtta aatcatttcc aatattggac cagtgtgtgg
     5161 gacaggttgg tctaaacttt gaactatctt ttactggggc tccaccatac tattataaca
     5221 ctaaaatcta taagcttgaa aacggtgaaa gaaagttata tgatgcaaag agatacacct
     5281 ctgaaggtac gagaaatcgc ttcagctata gtccacctaa agaaggtaac tatgaaatcg
     5341 tgtttgatac tgtttctaat aaattattca ctgaaccaat caaattggaa cctgtaaaag
     5401 aatatacttt caagacatca atgagggtga aaccaagcgc atcactaaaa ttacaccatg
     5461 atttgaaact ctgtttgggt gaccacagca gtgtcccagt agcactaaaa ggccaaggcc
     5521 cttttacgtt aacatatgat atcattgaaa ctttttctag caagaggaaa acttttgaaa
     5581 ttaaagaaat aaaaaccaac gaatatgtca ttaaaacacc agtctttact actggtggtg
     5641 attatattct atctttggtt tctatcaagg attctaccgg ctgtgtagtt ggactcagcc
     5701 aaccagatgc taaaatacag gtgaggagag atattccatc tgctgcattc aatttttttg
     5761 aacctatcaa agaagctaaa attaagcacg gttctgtcac tgaaattccg ctcaaattaa
     5821 gtggagaggg gccattcacc gttaagttta aacacatgga ttacgatgga aacattgtga
     5881 aagaatttga aaacaaattt cagaatagtt ataagcccgc tttgaaagta agtaaagaag
     5941 ggctttacca attggtcgat attcgtgact caagttgcca aggtaatgtg atttaccgga
     6001 acagtctgta taaggtatct ttcttggaga agccaaaatt tgcaattcaa gacaatcatc
     6061 acattacaaa agtaacggaa aatttattct ctaaagaaga agtctgtcaa gggatggaag
     6121 gtacggttga tttggctcta tttggttctc caccattcat attagaatat gatttgatgg
     6181 cacccaacgg tcatatttct acaaaaaaaa ttcaggtggc aaccaaatac gcttcactga
     6241 aactgcctaa tcaaattcct ggagagtaca taactactat taaggccatc ttcgatggca
     6301 attatggtga aagcgacata cattttagag aacatcagtc ggagctcata atcaaacaga
     6361 cagtgcaccc cattccagac gtcgcctttg ctgatggtgg taaaaccttg cgtgcatgtg
     6421 ctgctaatgt agatcaaatc tcatttttgg aaccaataaa tttgaaattt ttacaaggcg
     6481 aaagcccatt ttcaatcacc tttagcgttt atcacgagag taccagtagg acagatcagt
     6541 ataccattga caacattgac tcagaggctt tttcatttga aaagttatat gaagggatga
     6601 agttaggtaa ccatgccatt actattgatt ctgttgttga cgcaaatggt tgtgttaaca
     6661 gcctcatatc tggaccgcgt aatcaaattc ttgtgtcaat tactgatgca ccaaagatac
     6721 atatattaga tccttctacc gagtattgtg ttggggacta tgttgcatat caattaaacg
     6781 gtgtggcacc tttcatgatt aagtatgaat tcaacggtat tccactaaaa tctaaggaac
     6841 gcagctccca atttgttaga ttagcatctg aaccgggcat tatatctatc acttctttac
     6901 aggattcatc atcacaatgc attgtcgact ttacgaaccc taaattaaag agtgaatttg
     6961 atgacttatc tttaaacata catcctattc cttccgtaac tgtttctcaa ggaaattacg
     7021 ttactgagga tatccgcgaa ggtgaccagg ctgaagtaat cttttcgttt gaaggtaccc
     7081 caccattctc actaacttat gtaaggacag aagagactga tggaaaacac ggcaaaagga
     7141 ggtcgcaggt tgtygagacg cataaagtca ctgatatata ttctcatgaa tacaaggtaa
     7201 ttacaagctt gcaaggtacc tacgaggcga ttgaaattac agatgcttat tgttttgcca
     7261 aaaatgatct ttttttcaat aactaaatat tacaagaaag tctgatgaaa tttttctttc
     7321 ccgcaatatt tttgtacaat tgtaatgtat aatatatctc tgtacatcag tattaaaata
     7381 cttaagaaag cgaaaaagac gcctgtattg tttgttgttc tttttcttca aaattatcaa
     7441 gacgctcgat tcaattatct tcgctcatag taactcgaaa gagtgtaggt gaatgaatag
     7501 aaaaagagag cgctgagacc tttgtccttt acggtctata gaggtgtgtt aaaagcagac
     7561 ttttgaacat ccacagaaat tggctcaatg acctatccga aaagaatacc tataaatgcg
     7621 tggtctgaag ttcatagggt ggcaaggcca ctaattatta ctttcgacgc atataataca
     7681 ctatacgcca caaaacttcc cgtcatggaa caatactgca ttgttgggcg taaatatggt
     7741 atcaaggcga atccttcgac tttaacaaac aattttccac acgtttttaa aaaactgaag
     7801 gaagactatc cccaatacgg aaaatattct ggtatcaagc ccgaacaatg gtggtccatc
     7861 ttgattagga acgtcttcgc accaaacgaa attcccgatg agatgataaa tgaaatatta
     7921 atgcggtttg agggcttcga ttcgtatttt gtttatccag atttgatcaa atttttaaag
     7981 gatttgaagt caagacatcc tgatgttatc ttaggaatag taagcaatac tgatccaatt
     8041 ttttacaaat tattaaagaa tatcggatta tttgagacat tttccggtca catttattta
     8101 tcatatgagc ttaatctcgc caaacctgat agagcaatat ttcaatatgc gcttgacgat
     8161 attatatcta aacaacctca tttactcgaa aagtacacca gggaggaaat tctacagcat
     8221 tgctttcaca tcggagatga attaaagaac gatctcgaag gggcagaagc tgccggttgg
     8281 actggtatac tgttagatag aaatgataaa tacgggtttc tttcaaactc gattagtaaa
     8341 ccaatgcggg acgaatacaa gctgtcaatt gataaaatag ataataattc aataaacact
     8401 tgggaagcca atactaagca aactgacacg ttacagttga gtgaaagaaa gtacgttgtt
     8461 tcgaatcttg aggttttaga ggaactcttt ccctgagcgc ctaacgttac agtaacaaat
     8521 atgtatgata gatattattc atatccgcca gtaatattta ctatatcttt gaatgggttt
     8581 gtttattaat aatctactat gtgtaaatat atgatccaag tattatacag tttagacact
     8641 gattgttttc cagacgttca aaccgtcagt accggtactt actaaacaac ctgggatttg
     8701 cttatgccat ttgacatctt taacttcctt ttgccagtga acaaacaata attgtggtgg
     8761 aatttcttgt agctcttttg tttcggcggc ctgttgttta atttcctcat cgtcagcttc
     8821 tacagacaaa tcccacaaag tcacagtatt atcttctgag cctaccgcaa cgatagactc
     8881 atctaatggg ttgaatgcaa tggaagtaat ggctccctta tggaagtcat attgagcaac
     8941 cggttggacg gcgtcagcat tacttggcgt aaactgtctt aaatcccata ctccccatgt
     9001 accgttatcg tcaccgcttg ctagcaagta accaatttta tcactccaac ttatgacatt
     9061 tacgtcagta ttagaagctt taacagagat agcaggttta tgtttttttg atcttgtgtc
     9121 ccaaatcctt atatatccat cacatcctgc ggttgcaaaa acggtggatt cagtgcgaga
     9181 ccactggata tcttctatgg atttattgtt tgaaacagta aatggttgtt tatcagtcac
     9241 ccatctcgat gtgtgccttt gggtaaaata tatttgtcct gagcaatcac ctgatagtaa
     9301 cgcaccagtc ttgatcaatg gtgaccaatc caacccgtag ccttcaacat tcccatgatt
     9361 ttttacagtg tgaataggac gcttagcaga cttcggaatc tgataacctg gtgtggaaaa
     9421 agctttgctt tgtggagcta gattgtatat ataaacatca ccattttcgc tcattgtagc
     9481 ggttaacacc tcttgattag aaatggcaaa aggagaaacc tttaatctat tggttgtatc
     9541 tctcaatggt atattttcat tctcaatgac tggatccact agttctagag cggccgccac
     9601 cgcggtggag ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     9661 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9721 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9781 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9841 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     9901 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9961 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    10021 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    10081 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    10141 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    10201 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
    10261 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atcgaccggt
    10321 cgaggagaac ttctagtata tctacatacc taatattatt gccttattaa aaatggaatc
    10381 ccaacaatta catcaaaatc cacattctct tcaaaatcaa ttgtcctgta cttccttgtt
    10441 catgtgtgtt caaaaacgtt atatttatag gataattata ctctatttct caacaagtaa
    10501 ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg accgcagtta
    10561 actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag caaccattat
    10621 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
    10681 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
    10741 aattgggaga aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa
    10801 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
    10861 tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt cttacctttt
    10921 acatttcagc aatatatata tatatatttc aaggatatac cattctaatg tctgccccta
    10981 agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga aatcacagcc gaagccatta
    11041 aggttcttaa agctatttct gatgttcgtt ccaatgtcaa gttcgatttc gaaaatcatt
    11101 taattggtgg tgctgctatc gatgctacag gtgttccact tccagatgag gcgctggaag
    11161 cctccaagaa ggctgatgcc gttttgttag gtgctgtggg tggtcctaaa tggggtaccg
    11221 gtagtgttag acctgaacaa ggtttactaa aaatccgtaa agaacttcaa ttgtacgcca
    11281 acttaagacc atgtaacttt gcatccgact ctcttttaga cttatctcca atcaagccac
    11341 aatttgctaa aggtactgac ttcgttgttg tcagagaatt agtgggaggt atttactttg
    11401 gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga tagtgaacaa tacaccgttc
    11461 cagaagtgca aagaatcaca agaatggccg ctttcatggc cctacaacat gagccaccat
    11521 tgcctatttg gtccttggat aaagctaatg ttttggcctc ttcaagatta tggagaaaaa
    11581 ctgtggagga aaccatcaag aacgaattcc ctacattgaa ggttcaacat caattgattg
    11641 attctgccgc catgatccta gttaagaacc caacccacct aaatggtatt ataatcacca
    11701 gcaacatgtt tggtgatatc atctccgatg aagcctccgt tatcccaggt tccttgggtt
    11761 tgttgccatc tgcgtccttg gcctctttgc cagacaagaa caccgcattt ggtttgtacg
    11821 aaccatgcca cggttctgct ccagatttgc caaagaataa ggtcaaccct atcgccacta
    11881 tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt gcctgaagaa ggtaaggcca
    11941 ttgaagatgc agttaaaaag gttttggatg caggtatcag aactggtgat ttaggtggtt
    12001 ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga agttaagaaa atccttgctt
    12061 aaaaagattc tcttttttta tgatatttgt acataaactt tataaatgaa attcataata
    12121 gaaacgacac gaaattacaa aatggaatat gttcataggg tagacgaaac tatatacgca
    12181 atctacatac atttatcaag aaggagaaaa aggaggatgt aaaggaatac aggtaagcaa
    12241 attgatacta atggctcaac gtgataagga aaaagaattg cactttaaca ttaatattga
    12301 caaggaggag ggcaccacac aaaaagttag gtgtaacaga aaatcatgaa actatgattc
    12361 ctaatttata tattggagga ttttctctaa aaaaaaaaaa atacaacaaa taaaaaacac
    12421 tcaatgacct gaccatttga tggagtttaa gtcaatacct tcttgaacca tttcccataa
    12481 tggtgaaagt tccctcaaga attttactct gtcagaaacg gccttaacga cgtagtcgat
    12541 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
    12601 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    12661 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    12721 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    12781 ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg
    12841 gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag
    12901 aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt
    12961 aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt
    13021 aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt
    13081 ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca
    13141 agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa
    13201 aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt
    13261 aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt
    13321 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    13381 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    13441 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    13501 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    13561 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    13621 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    13681 gtattg
//
