LOCUS       sequence-368129-        4011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC16A12_STOP
FEATURES             Location/Qualifiers
     source          1..4011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1568..1667)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1684..1703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1685..1703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1708..1724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1708..1724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1826..1845)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1837..2646
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1904..1923)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2793..3381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3282..3301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3535..3552
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3711..3738)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3830..3916)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3965..3987
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3979..3996
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3980..3996
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc caaggtgaac
      121 agggcaagat ctaccagccc acctgacgga ggatggggat ggatgatcgt ggcaggctgt
      181 ttcctggtga ccatctgcac acgggccgtg accagatgta tcagcatctt ctttgtggag
      241 ttccagacct actttacaca ggactatgcc cagaccgcct ggatccactc tatcgtggat
      301 tgcgtgacaa tgctgtgcgc accactggga agcgtggtgt ccaatcacct gagctgtcaa
      361 gtgggaatca tgctgggagg actgctggca agcaccggac tgatcctgag ctccttcgcc
      421 acatccctga agcacctgta cctgaccctg ggcgtgctga caggactggg attcgccctg
      481 tgctattctc cagccatcgc catggtgggc aagtacttta gccggagaaa ggccctggcc
      541 tatggcatcg ccatgtccgg ctctggcatc ggcaccttta tcctggcccc cgtggtgcag
      601 ctgctgatcg agcagttcag ctggaggggc gccctgctga tcctgggagg cttcgtgctg
      661 aacctgtgcg tgtgcggcgc cctgatgaga cctatcaccc tgaaggagga ccacaccaca
      721 ccagagcaga atcacgtgtg caggacacag aaggaggata tcaagcgcgt gtccccttac
      781 tctagcctga ccaaggagtg ggcccagaca tgcctgtgct gttgcctgca gcaggagtat
      841 agcttcctgc tgatgtccga ctttgtggtg ctggccgtgt ccgtgctgtt catggcctac
      901 ggctgctctc cactgttcgt gtacctggtg ccctatgccc tgtctgtggg cgtgagccac
      961 cagcaggcag ccttcctgat gtccatcctg ggcgtgatcg acatcatcgg caacatcacc
     1021 tttggctggc tgacagatag gcgctgtctg aagaattacc agtacgtgtg ctacctgttc
     1081 gccgtgggca tggacggcct gtgctatctg tgcctgccta tgctgcagtc tctgcctctg
     1141 ctggtgccct tcagctgtac cttcggctac tttgatggcg cctatgtgac actgatcccc
     1201 gtggtgacca cagagatcgt gggcaccaca agcctgtcct ctgccctggg cgtggtgtac
     1261 ttcctgcacg ccgtgcctta tctggtgtcc ccaccaatcg caggcaggct ggtggatacc
     1321 acaggctcct acaccgccgc ctttctgctg tgcggcttct ctatgatctt tagctccgtg
     1381 ctgctgggat tcgcaaggct gatcaagagg atgcgcaaga cccagctgca gtttatcgcc
     1441 aaggagtccg acccaaagct gcagctgtgg acaaacggct ccgtggccta ttctgtggcc
     1501 cgggagctgg atcagaagca cggagagcca gtggcaaccg cagtgcctgg ctactctctg
     1561 acataagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1621 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     1681 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1741 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1801 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1861 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1921 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1981 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2041 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2101 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2161 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2221 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2281 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2341 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2401 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2461 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2521 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2581 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2641 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2701 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2761 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2821 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2881 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2941 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3001 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3061 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3121 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3181 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3241 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3301 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3361 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3421 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3481 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3541 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3601 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3661 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3721 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3781 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3841 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3901 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3961 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
