LOCUS       sequence-36814-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu 
            S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC
  TITLE     Harnessing a high cargo-capacity transposon for genetic applications
            in vertebrates.
  JOURNAL   PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.
  PUBMED    17096595
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2006-11-10"; volume: "2(11)"; pages: "e169. Epub 
            2006 Aug 28"
COMMENT     Sequence Label: pCMV-Tol2
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          150..246
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      302..311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cggggcnnng cgactgttng tgaccgtcag atcgcctgga gacgccatcc acgctgtttt
       61 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagagga tccggtactc
      121 gaggaactga aaaaccagaa agttaactgg taagtttagt ctttttgtct tttatttcag
      181 gtcccggatc cggtggtggt gcaaatcaaa gaactgctcc tcagtggatg ttgcctttac
      241 ttctaggcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg tacccgcggc
      301 cgccaccatg gaggaagtat gtgattcatc agcagctgcg agcagcacag tccaaaatca
      361 gccacaggat caagagcacc cgtggccgta tcttcgcgaa ttcttttctt taagtggtgt
      421 aaataaagat tcattcaaga tgaaatgtgt cctctgtctc ccgcttaata aagaaatatc
      481 ggccttcaaa agttcgccat caaacctaag gaagcatatt gagagaatgc acccaaatta
      541 cctcaaaaac tactctaaat tgacagcaca gaagagaaag atcgggacct ccacccatgc
      601 ttccagcagt aagcaactga aagttgactc agttttccca gtcaaacatg tgtctccagt
      661 cactgtgaac aaagctatat taaggtacat cattcaagga cttcatcctt tcagcactgt
      721 tgatctgcca tcatttaaag agctgattag tacactgcag cctggcattt ctgtcattac
      781 aaggcctact ttacgctcca agatagctga agctgctctg atcatgaaac agaaagtgac
      841 tgctgccatg agtgaagttg aatggattgc aaccacaacg gattgttgga ctgcacgtag
      901 aaagtcattc attggtgtaa
//
