LOCUS       sequence-368140-        8537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Belanger KD, Griffith AL, Baker HL, Hansen JN, Kovacs LA, Seconi JS,
            Strine AC
  TITLE     The karyopherin Kap95 and the C-termini of Rfa1, Rfa2, and Rfa3 are 
            necessary for efficient nuclear import of functional RPA complex 
            proteins in Saccharomyces cerevisiae.
  JOURNAL   DNA Cell Biol. 2011 Sep;30(9):641-51. doi: 10.1089/dna.2010.1071. 
            Epub 2011 Feb 20.
  PUBMED    21332387
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/dna.2010.1071"; journalName: "DNA Cell Biol"; date: 
            "2011-09"; volume: "30"; issue: "9"; pages: "641-51"
COMMENT     Sequence Label: KBB438
FEATURES             Location/Qualifiers
     source          1..8537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    35..538
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     579..597
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(635..657)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     757..776
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        800..1015
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             1016..1819
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1950..2405)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2087..2108)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2299..2318
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2531..2553
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2545..2562
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2546..2562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2572..2591
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2572..2590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2609..2856)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     2822..2840
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     2858..2874
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2859..2875
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(3253..3300)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     3296..3314
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(3365..4075)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3405..3425)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     4020..4048
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(5938..5961)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(6370..6390)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6370..6388)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6409..6425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6409..6425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6422..6444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6433..6449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6457..6463,6464..6481,6482..6487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6502..6523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6640..6657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6811..7399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6891..6910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7570..8361,8362..8430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8193..8212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8431..8535)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa
       61 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
      121 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
      181 taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
      241 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
      301 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
      361 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc
      421 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt
      481 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg
      541 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
      601 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      661 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      721 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
      781 gagtgcacca taccaccttt tcaattcatc attttttttt tattcttttt tttgatttcg
      841 gtttccttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
      901 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
      961 agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc
     1021 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     1081 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     1141 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     1201 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     1261 caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa
     1321 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     1381 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     1441 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     1501 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     1561 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     1621 cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga
     1681 tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     1741 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     1801 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     1861 aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa ataccgcaca
     1921 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     1981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     2041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     2101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     2161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     2221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     2281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     2341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     2401 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     2461 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     2521 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     2581 tcactatagg gcgaattggg taccggccgc aaattaaagc cttcgagcgt cccaaaacct
     2641 tctcaagcaa ggttttcagt ataatgttac atgcgtacac gcgtctgtac agaaaaaaaa
     2701 gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa aaataaatag
     2761 ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat gtggggggag
     2821 ggcgtgaatg taagcgtgac ataactaatt acatgactcg aggtcgacgg tatcgataag
     2881 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
     2941 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
     3001 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc
     3061 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt
     3121 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
     3181 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatatc cggatatagt
     3241 tcctcctttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag
     3301 ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt tagcagccgg
     3361 atcctttgta tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa
     3421 ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt
     3481 aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt
     3541 ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta gctttgattc
     3601 cattcttttg tttgtctgcc atgatgtata cattgtgtga gttatagttg tattccaatt
     3661 tgtgcccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg attctattaa
     3721 caagggtatc accttcaaac ttgacttcag cacgtgtctt gtagttcccg tcatctttga
     3781 aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgcc
     3841 gtttcatatg atccgggtat cttgaaaagc actgaacacc ataagtgaaa gtagtgacaa
     3901 gtgttggcca tggaacaggt agttttccag tagtgcaaat aaatttaagg gtaagttttc
     3961 cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca ttaacatcac
     4021 catctaattc aacaagaatt gggacaactc cagtgaagag ttcttctcct ttgctagcta
     4081 acaaagcctt ggataactca tcggcaagat agtcggcttc agccctgtaa ttcaagctgt
     4141 gtaggttagc aacggtatat ctaattctgc tttgatcatt gtatgtatcc tcacgcgctc
     4201 taatcctaaa gtcatattcg ttcatttgga tactttgagt aatttttgtg aattcgttgg
     4261 ggtcttcttc cttcaaagac attaatgtat tagcatcaac acccaataat tgtttagctt
     4321 ggtcgtcaaa taaagtgagc catagttgat tggtttcgtc aataattgat attgtcaaga
     4381 tgtatctcca atttggcctt gcattattgg tgtcgcactt ctcacatctc caagtaccat
     4441 caggctgttc cagaactttc ttattacaat tctcattaga acaggcagga tatgcaaaat
     4501 tatcaacttt taagaaactt atagcagctt taacactaaa aaagtcacct ttctcgcttc
     4561 ttcctagatt ttcagcttga gctctagcaa tagtaatacg ctgagcaatg aattttgtta
     4621 agctagcagc cgattgacca cccataccgg gttcttgctt taaagtgatg aagtttgcgt
     4681 tgcggccctt ggaatcatac caacccttta aggcatatgc ctcaggaatt tctggattcg
     4741 gaatcagggt actagaaaat cccatagaca aagatttgcc accaaaatcc gtcacacgaa
     4801 cacctttaat ggcagcaaca gaaccttcag gaaggttgaa atcaagggct tgctgattcc
     4861 ataggccaac agagatagaa aacccagagt cgtcaacaat tgtgatgtcc ccagcccttg
     4921 aagttagctc aaaatgtggg tttatagttt ggataatacc gaggacgtct acgttggaat
     4981 ttacttcctg gttctgaata gcatctagtt tgatgaaatt gaaatgggtt ttcggaacat
     5041 tactttcatc gaaacattct tctataacag tgtctctatc caaattcagt tcataagggt
     5101 gtgttagatt agtaaattgg ggcttagctg gttggagttt tgcctttgat acatagtata
     5161 ctttgccttc ttgtaaaatt tcgttaaatt ttgtagcaaa atcattaaac gccgtggctc
     5221 ggatttctcc agaggtatcc aagaagttga cattgaatag tttaccatca cctctttgat
     5281 tgtgccacgt tttaatttct cccttgtagg aaactcttgc tttgatagtc caaacgtttt
     5341 ggtatggaga cagttgttcg atggcaaaaa ttggtctggt tttttgcgaa ttagggtttt
     5401 cattggcgaa ttttctctca tttgcattca agtttgagtt tgaatgcagc atatcaggga
     5461 caccagcatt gctggcgttt gtttgattgg caacattacc actgtcagtt atatcttcgt
     5521 cttttaaggt ttcatttgga tgctctgaga aatagttatc caaaaaagta ctagtttggt
     5581 tgaccatatc agcacgcgac tggaccaact caaagtcatc tactaaaaga acgtatttct
     5641 ttctttccct gacaatagca ggttctgcaa ttatcacgcg aatgatatca cccctttgta
     5701 gttccattga ctggaacttg gatgcagctt ggtttctcaa cagagccttc atatggtaaa
     5761 taccatcgga aatcatgatc aaattctttc tgttgctgtt agccccatca gatttcctgg
     5821 tgttataaac ttgataaacg ccaccggtgg gattatcgta cctttgctta ttggtgaaga
     5881 tgctatgaaa atcgcccctc gaaagttgaa cactgctcat tatagttttt tctccttgac
     5941 gttaaagtat agaggtatat taacaatttt ttgttgatac ttttattaca tttgaataag
     6001 aagtaataca aaccgaaaat gttgaaagta ttagttaaag tggttatgca gtttttgcat
     6061 ttatatatcc gttaatagat caaaaatcat cgcttcgctg attaattacc ccagaaataa
     6121 ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa tttgattcgt tcatttgaag
     6181 gtttgtgggg ccaggttact gccaattttt cctcttcata accataaaag ctagtattgt
     6241 agaatcttta ttgttcggag cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac
     6301 cggtgaagac gaggacgcac ggaggagagt cttccttcgg agggctgtca cgagctccag
     6361 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     6421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8521 aataggggtt ccgcgca
//
