LOCUS       sequence-368142-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NMA111-GFP wild type under control of GAL1 promoter (complements 
            nma111 deletion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Belanger KD, Walter D, Henderson TA, Yelton AL, O'Brien TG, Belanger
            KG, Geier SJ, Fahrenkrog B
  TITLE     Nuclear localisation is crucial for the proapoptotic activity of the
            HtrA-like serine protease Nma111p.
  JOURNAL   J Cell Sci. 2009 Nov 1;122(Pt 21):3931-41. doi: 10.1242/jcs.056887. 
            Epub 2009 Oct 6.
  PUBMED    19808885
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2009-11-1"; volume: "122(Pt 21):3931-41. doi"; pages: 
            " 10.1242/jcs.056887. Epub 2009 Oct 6"
COMMENT     Sequence Label: KBB439
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        419..523
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(524..592,593..1384)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(742..761)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1555..2143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2044..2063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2297..2314
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2431..2452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2467..2472,2473..2490,2491..2497)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2505..2521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2510..2532
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2529..2545
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2529..2545
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2564..2584
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2566..2584
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2993..3016
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             6028..6738
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(6055..6083)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6678..6698
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6789..6807)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6803..6850
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7228..7244)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7229..7245)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      7247..7494
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7263..7281)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7512..7531)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7513..7531)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7541..7558)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7541..7557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7550..7572)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7698..8153
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7785..7804)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7995..8016
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8284..9087)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9088..9303)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9327..9346)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9446..9468
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9506..9524)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(9565..9686,1..382)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
       61 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
      121 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
      181 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
      241 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt
      301 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat
      361 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg
      421 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      481 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      541 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      601 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      661 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      721 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      781 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      841 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      901 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      961 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1021 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1081 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1141 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1201 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1261 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1321 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1381 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1441 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1501 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1561 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1621 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1681 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1741 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1801 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1861 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1921 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1981 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2041 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2101 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2161 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2221 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2281 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2341 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2401 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2461 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2521 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     2581 aagggaacaa aagctggagc tcgtgacagc cctccgaagg aagactctcc tccgtgcgtc
     2641 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
     2701 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
     2761 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
     2821 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
     2881 aacggatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
     2941 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
     3001 tctatacttt aacgtcaagg agaaaaaact ataatgacca tatcgttgag caatataaag
     3061 aaaagagacc attctaaaat ttccgatgga acttctggtg aatcatcgct tgtaaaaagg
     3121 aaacagttgg aaagcgccac cggagatcaa gaggaagaat atacagacca tgaaatcatt
     3181 atagagccgt tgcactttgc taataataat aacactgtac tcacagattc agaaaattat
     3241 ctaagatggc aaaataccat ctccaacgtt gttaaatcag tggtttccat tcatttttca
     3301 caagttgcgc cattcgattg cgattctgct ttggtatctg aagctaccgg atttgtcgtg
     3361 gatgctaaat taggtataat tctgaccaat aggcacgtcg ttggtcctgg tccgttcgta
     3421 ggctacgtag ttttcgataa tcatgaagaa tgcgatgtta taccaattta cagagatcct
     3481 gttcatgatt ttgggttcct caaattcgat ccgaaaaaca taaaatattc taagatcaaa
     3541 gcgttaacgc taaaaccaag tttggctaag gtcggctcag aaattagagt tgtcggtaat
     3601 gatgccggtg agaaattaag tatcctagca ggttttatta gtagaattga caggaacgct
     3661 ccagaatatg gtgagctgac atacaatgac ttcaatacag aatatattca agctgctgca
     3721 tctgcttctg gcggttcaag tggttctcca gtcgtaaata tagatgggta tgctgtggct
     3781 ttacaggcag gtggctctac agaggcatct actgattttt ttttaccgtt agatagaatt
     3841 cttagagcgt taatttgcat ccaaaccaac aaacctatca cacgtggtac gattcaagtt
     3901 caatggctgc tgaagcctta tgacgagtgt agaagattag gtttaacttc agagagagaa
     3961 agtgaggctc gtgctaaatt tcccgaaaat atcggactgt tggtagcaga aaccgtttta
     4021 agagaaggcc caggctatga taaaattaaa gaaggtgata ctctaatttc aataaacggt
     4081 gaaaccatat cttctttcat gcaagtggat aaaattcaag atgaaaatgt tgggaaggaa
     4141 atccaattgg ttattcaaag gggcggtgtc gaatgtactg tcacctgcac tgttggtgat
     4201 ttacatgcta tcacccctca tcgttatgta gaggtttgtg gtgcgacttt tcatgaatta
     4261 tcatatcaaa tggcaaggtt ttatgcgcta cctgtcaggg gtgtattttt aagtagtgca
     4321 tcagggtctt tcaactttga ttcaaaagaa agagtgggtt ggattgtgga ttctatagac
     4381 aataaggaaa cccccgactt agatacattt attgaaatta tgaaaactat tccagatcgt
     4441 aagcgcgtta ctgtacgata ccaccatcta acagatcagc attctccact tgttacttct
     4501 atttacattg accgtcattg gtgcaatgaa ttcagagttt acacaaggaa cgatacaact
     4561 ggtatttggg attacaaaaa tgttgctgat ccacttccag ccgatgcgtt gaaacctcgt
     4621 tcggccaaaa ttattcctat tcctgttaat aatgaaaaag ttgcaaaatt gtcatcatcg
     4681 ttatgtacag tggctacaat ggcagcagtt ccattggatt cactttctgc tgatatttta
     4741 aaaacatcag gcttaatcat tgatgctgaa aaaggttatg tcctagtgtc cagaagggta
     4801 gttccacatg attgtcttga tacttttgtc accattgcag attcccttgt agttccagcc
     4861 acagtagaat ttctccatcc aactcacaac ttcgctattg ttaaatatga tccagagtta
     4921 gtaaaggctc cactaatcac tcccaagcta tctaccacca gaatgaaacg tggtgataag
     4981 cttcagttta taggttttac ccagaatgat agaattgtta cttcagagac cactgtcacc
     5041 gatatttcct ctgtaagtat tccaagtaat ttaattccga ggtaccgtgc cactaattta
     5101 gaagctatat ctatcgactg caacgttagc accagatgta attccggtat tttgacagat
     5161 aatgatggta cagtaagagg tttatggctt ccatttttag gcgaaagatt agaaaataaa
     5221 gaaaaggttt atttgatggg attagatata atggactgta gggaagttat agatatctta
     5281 aaaaatggcg gtaaaccaag agtaagtata gttgatgctg ggttcggttc aatttccgtt
     5341 ctgcaagcca gaattagagg tgtgcccgaa gaatggatca tgcgtatgga acatgaatca
     5401 aataacaggc tacagtttat cactgtttct agagtttctt atacagaaga caagattcat
     5461 ttagagaccg gtgacgtcat tttatctgtg aacggaaaat tagtcactga aatgaatgat
     5521 ttgaatggag ttgtaagctc agctgatggt attttgccat cagccatgtt agattttaaa
     5581 gttgttcgtg atggtaatat tgttgatttg aaaattaaaa ccgtggaagt tcaggaaacc
     5641 gaccgttttg taatatttgc aggtagtatc ttacaaaagc cgcatcatgc tgttttgcaa
     5701 gctatggttg atgttccaaa gggtgtctac tgtacattca ggggagaatc gtcaccggcg
     5761 ctccagtacg gtatatctgc cacaaacttc ataactcatg tcaatgaaat tgaaacacct
     5821 gatctagata catttttgaa agttgtcaaa acaatccctg ataatagtta ttgtaaaatg
     5881 aggttaatga ctttcgacaa tgtgcccttc gctatttcac tgaaaacgaa ttatcattac
     5941 ttccctactg cagaattgaa gagggacaac atcacgcaca aatggattga aaaggaattt
     6001 accggcaaca gccaaagtga aaaagctagc aaaggagaag aactcttcac tggagttgtc
     6061 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     6121 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     6181 ctacctgttc catggccaac acttgtcact actttcactt atggtgttca gtgcttttca
     6241 agatacccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
     6301 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     6361 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     6421 gatggaaaca ttcttgggca caaattggaa tacaactata actcacacaa tgtatacatc
     6481 atggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattagaca caacattgaa
     6541 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     6601 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     6661 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     6721 atggatgaac tatacaaagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     6781 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     6841 gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca
     6901 tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa
     6961 agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg
     7021 ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc
     7081 ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga
     7141 tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact
     7201 gtgataaact accgcattaa agcttatcga taccgtcgac ctcgagtcat gtaattagtt
     7261 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
     7321 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
     7381 ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt
     7441 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggccgg
     7501 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7561 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7621 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7681 gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7741 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7801 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7861 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7921 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7981 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8041 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8101 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg
     8161 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata
     8221 taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt
     8281 tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg
     8341 ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat
     8401 gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc
     8461 cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc
     8521 acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc
     8581 aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa
     8641 catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac
     8701 aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta
     8761 tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc
     8821 gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat
     8881 ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc
     8941 ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt
     9001 ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc
     9061 acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg tttttgttct
     9121 gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat
     9181 atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga
     9241 atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt
     9301 gaaaaggtgg tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9361 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9421 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     9481 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9541 atgataataa tggtttctta gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct
     9601 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt
     9661 tggattttag aaagtaaata aagaag
//
