LOCUS       sequence-368144-        5025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  It's an empty replication plasmid backbone  in Bacteroides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Zheng L, Tan Y, Hu Y, Shen J, Qu Z, Chen X, Ho CL, Leung EL, Zhao W,
            Dai L
  TITLE     CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides 
            Species.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):464-472. doi: 
            10.1021/acssynbio.1c00543. Epub 2022 Jan 6.
  PUBMED    34990118
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00543"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "464-472"
COMMENT     Sequence Label: pB006
FEATURES             Location/Qualifiers
     source          1..5025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(30..821,822..890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     653..672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(4207..4223)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4207..4223)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     rep_origin      4256..4644
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            4815..4924
                     /note="incP origin of transfer"
ORIGIN
        1 acgcgttatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
       61 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      121 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      181 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      241 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      301 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      361 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      421 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      481 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
      541 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
      601 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
      661 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
      721 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
      781 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      841 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      901 tttcaatcat ggccgcggga ttaaaagtcg gggattggtg aacaaaaagg tgtttctctc
      961 tttaagagaa atatcgtttt gctaaacagt tgatattgag gtatcatttt atcgtaaaag
     1021 acatttttgc tcaacaattg cttgacggaa atcaacaaat tttagcattt tgtaaaaaag
     1081 tcgctatata atttggtgaa ttggagttat tttcatattt ttgcatcccg aagagtttct
     1141 cttaaagaga gaaacatctt ttgcatacct tttccgaccg aatttttatg tcgtaaagag
     1201 gggctttgca gggggtggac tcagaaagat gagaatagat gactattgta gttgaaacac
     1261 atagaaagtt gctgatatac agaccgatac gcatatcggg atgaaccatg agtacgttct
     1321 tttctcaaaa aacataaata ttcgaaaaga gatgcaataa attaaggaga ggttataatg
     1381 aacaaagtaa atataaaaga tagtcaaaat tttattactt caaaatatca catagaaaaa
     1441 ataatgaatt gcataagttt agatgaaaaa gataacatct ttgaaatagg tgcagggaaa
     1501 ggtcatttta ctgctggatt ggtaaagaga tgtaattttg taacggcgat agaaattgat
     1561 tctaaattat gtgaggtaac tcgtaataag ctcttaaatt atcctaacta tcaaatagta
     1621 aatgatgata tactgaaatt tacatttcct agccacaatc catataaaat atttggcagc
     1681 ataccttaca acataagcac aaatataatt cgaaaaattg tttttgaaag ttcagccaca
     1741 ataagttatt taatagtgga atatggtttt gctaaaatgt tattagatac aaacagatca
     1801 ctagcattgc tgttaatggc agaggtagat atttctatat tagcaaaaat tcctaggtat
     1861 tatttccatc caaaacctaa agtggatagc acattaattg tattaaaaag aaagccagca
     1921 aaaatggcat ttaaagagag aaaaaaatat gaaacttttg taatgaaatg ggttaacaaa
     1981 gagtacgaaa aactgtttac aaaaaatcaa tttaataaag ctttaaaaca tgcgagaata
     2041 tatgatataa acaatattag tttcgaacaa tttgtatcgc tatttaatag ttataaaata
     2101 tttaacggct aaaaacaata ggccacatgc aactgtaaat gtttacgcgt cctcggtacc
     2161 gcttcttcca caacagtctg cggttcctgt actatcacag gttcatcttc tcccggtgct
     2221 gtcgcttcat tctcttccac gaccttctct tctatcaccg gacattcaac ttcctctatt
     2281 acaggggctt gttcttcaac aatgggggct tcactttccg cttccgctac aggagaaggc
     2341 gaggcttcca ccggcacagc acttatctct tccgacaact gttccaaacg ctcctgcata
     2401 cgtaggatgc tccgcttcaa cagttcagac aaagtctgag tcggctcttt agaaaacgta
     2461 ttcatgagta gcttcagctc atgaacatcc agctcaatat ctgttaatag cttctgtttc
     2521 atttccatcc attgttatta tggtgcgaat gtaggaataa tttatgcaat aacttaaaat
     2581 aatggataat ttaacagttc ttgtacatcg aattgtttac ctttgtccac tcatttattt
     2641 aatcaagaaa gcacaaaaat cacaagatta cccggagtgt ttcagccggg taatcttgtt
     2701 acttatcctt gattttccct ttaagggcgt ttataatcca ccctttcgga ttgttctttt
     2761 ctcgtgattt tccgtttagg agagccagtt ctccgataag gtcggttatc ttttcttgtg
     2821 ccgttatgaa tgtctctttg ttccggttta tctcttccga tgtgaagccg caggaataac
     2881 ggaggtactc gtacacatgg ctgtctatct gatatcgtgc tgtaaccttt gcttgcaatt
     2941 ctttcccttc cagttcttca tctctgaact gtggttgata gaccgggtag aacctaaacc
     3001 cggtcacctt gctacgtttg ttgtttgggt tttcacgcac tttcacgtag ttgaaagtgt
     3061 atgggcagct ctcatcaagc gcagcctttg ctggtttcaa aactctttcc tcaaaatgat
     3121 ctattctgtc ttttccgttc ttgtccttgt atttgtccgc aggtatgcca agacggtctt
     3181 tcaagttttc aagggatata tccaaagggt acacctgccc actcattaac atgtaaaacc
     3241 taagggaata cccagtaggt agcgcaagag ccttgtttaa ctcaaattct ctgtacccct
     3301 tggcgaactt ggtaaacaca tcccaaaggt cgttagacac acgaaaggtt ataatgccag
     3361 tgcgtttttt atattttggg ttagatataa atccgcactt ccaccattcc tcgtcatctt
     3421 cgtaagtaaa aaaacgtcct gccaaagaat cgagtgcggc tatgatttca ttgtaatcac
     3481 gtccggaaaa aataacgtca gatacatgca tttgtgcatc aacatcccaa agtccatgct
     3541 caaacttgcg tttcgtgcct gcataatctt tcagttttac tccctttagt tcagcttggc
     3601 acgcttccag tatgcgaaga actattcttt gttcgtgtat ggacatgtct tgctgttttg
     3661 accatgtata tacccatgaa acaacaacat ctttattctt ggtaataggt agtttctttt
     3721 tcataaacga ttgatttgct gcaaatatac acaatcccca cattaaaaca aaaaaagagg
     3781 ggtaaaactc ccaaagtgtg gggactaatt tacttttaaa ctcccaaagt gtggggacta
     3841 aactcccaaa gtgtggggaa acgcatgttc taatcccttt atttatgggc gtttgcgaag
     3901 aagtgataga gcatattaca gacgatgctt ataacagaca aggggaattt atagagctaa
     3961 gtggttgatt tactgtcgga aaaagaaaaa cgatagggaa aaagagcaaa gcaaaaccaa
     4021 ccaacgggcg gtaggattgg aaacgggttt ttatggcgtg tacacgtttg tgtacaaaat
     4081 gtttgtattt tattgaaata tagctatctt tgtgctgaat ttaaaagcaa acagatggaa
     4141 agaaagaaga taacattcga gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
     4201 ccgggggatc cactagttct agagcggccg ccaccgcggt ggaggggaat tcccatgtca
     4261 gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg gcttctcagt
     4321 gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta
     4381 tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta
     4441 tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt
     4501 tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta
     4561 gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt actatcaaca
     4621 ggttgaactg ctgatcttca gatcctctac gccggacgca tcgtggccgg atcaattccg
     4681 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     4741 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     4801 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     4861 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     4921 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc
     4981 aggaagggca gcccacctat cacggaattg atccccctcg aattg
//
