LOCUS       sequence-368146-        4719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hyperfolder YFP fluorescent protein: bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Campbell BC, Paez-Segala MG, Looger LL, Petsko GA, Liu CF
  TITLE     Chemically stable fluorescent proteins for advanced microscopy.
  JOURNAL   Nat Methods. 2022 Nov 7. pii: 10.1038/s41592-022-01660-7. doi: 
            10.1038/s41592-022-01660-7.
  PUBMED    36344833
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 Nov 7. pii: 10.1038/s41592-022-01660-7. doi: 
            10.1038/s41592-022-01660-7."
COMMENT     Sequence Label: pBAD-hfYFP
FEATURES             Location/Qualifiers
     source          1..4719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        45..329
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     252..271
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             372..389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(444..465)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1164..1181)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1334..1420
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1512..1539
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1558..1649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1650..1718,1719..2510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1868..1887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2681..3269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3170..3189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3423..3440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3455..3595
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3521..3540)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3681..3703
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(3859..4719,1..18))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc
       61 atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc
      121 ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac
      181 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
      241 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      301 tttatcgcaa ctctctactg tttctccata cccgtttttt gggctaacag gaggaattaa
      361 ccatggggag ccaccatcac catcaccatg gcagatctat ggtgagcaag ggcgaggagc
      421 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      481 tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagctca
      541 tctccaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttaggctacg
      601 gcctgatggt gttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      661 ccatgcccga aggctacgtc caggagcgca ccatctcttt cgaggacgac ggttactaca
      721 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gtgctgaagg
      781 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
      841 gccacaacgt ctatatcacg gccgacaagc agaagaacgg catcaaggct aacttcaaga
      901 tccgccacaa cgttgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
      961 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgtcc
     1021 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gaaggagagg gtgaccgccg
     1081 ccgggattac acatgacatg aacgagctgt acaagtaaga attcgaagct tggctgtttt
     1141 ggtggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     1201 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     1261 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
     1321 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1381 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     1441 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     1501 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt
     1561 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1621 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1681 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1741 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1801 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1861 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     1921 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1981 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2041 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2101 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2161 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2221 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2281 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2341 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2401 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2461 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2521 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2581 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2821 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3361 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3421 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3481 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     3541 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     3601 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3661 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3721 gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc
     3781 ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat
     3841 tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca
     3901 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
     3961 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
     4021 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
     4081 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     4141 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     4201 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
     4261 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     4321 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
     4381 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
     4441 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     4501 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
     4561 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     4621 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     4681 cagatgggca ttaaacgagt atcccggcag caggggatc
//
