LOCUS       sequence-368147-       12365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FnCas12a in Bacteroides and used for genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Zheng L, Tan Y, Hu Y, Shen J, Qu Z, Chen X, Ho CL, Leung EL, Zhao W,
            Dai L
  TITLE     CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides 
            Species.
  JOURNAL   ACS Synth Biol. 2022 Jan 21;11(1):464-472. doi: 
            10.1021/acssynbio.1c00543. Epub 2022 Jan 6.
  PUBMED    34990118
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00543"; journalName: "ACS Synth Biol"; date: 
            "2022-01-21- 21"; volume: "11"; issue: "1"; pages: "464-472"
COMMENT     Sequence Label: pB025
FEATURES             Location/Qualifiers
     source          1..12365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(9676..9744,9745..10536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTSLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9894..9913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            10667..10776
                     /note="incP origin of transfer"
     rep_origin      10947..11335
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(11362..11985)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     11893..11912
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    12256..12274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    12318..12336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atgagcatct atcaagaatt tgtgaataaa tatagtctta gtaaaacatt gagattcgaa
       61 ctgatcccgc aaggaaaaac tctggaaaat attaaagcac gtggattgat tttggatgat
      121 gaaaaacgtg ctaaagatta taaaaaagcc aaacagataa ttgataaata tcatcaattc
      181 ttcattgaag aaatccttag tagtgtatgc attagcgaag atctgcttca gaattatagt
      241 gatgtatatt ttaaactgaa aaaatctgat gatgataatt tacagaaaga ctttaaatca
      301 gctaaagata caataaagaa acaaattagt gaatatatta aagatagtga aaagtttaaa
      361 aatttgttta accagaatct gatcgatgct aaaaaaggac aggaatccga tctgattctg
      421 tggttgaaac agagtaaaga taatggtatt gaattgttta aagccaattc tgatatcact
      481 gacattgatg aagcactgga aattatcaaa tcctttaaag gttggactac ctattttaaa
      541 ggttttcacg aaaatcgtaa aaatgtgtat tctagtaatg atataccgac gtcgattatc
      601 tatcgtatcg tagatgataa tctgccgaaa tttctggaaa ataaagctaa atacgaatct
      661 ctgaaagata aagcacccga agccatcaat tatgaacaga ttaagaagga tctggctgaa
      721 gaactgacat ttgacataga ttataaaacg tctgaagtaa atcagcgcgt atttagtctg
      781 gatgaagtgt tcgaaatcgc aaattttaac aattacttaa atcagtcggg tataaccaag
      841 tttaatacca taatcggagg aaaatttgta aatggagaaa atacgaaacg caaagggatc
      901 aatgaatata tcaatttgta ttctcagcag attaacgata aaacattgaa aaaatataaa
      961 atgagtgtac tgttcaaaca aatcctgtct gataccgaat ctaaaagttt cgtaatcgat
     1021 aaattagaag atgattctga tgtggtgact acaatgcaga gcttctatga acagatcgct
     1081 gcattcaaaa ctgtagaaga aaagagtatt aaggaaacac tgtctttgct gtttgatgat
     1141 ctgaaagctc agaaacttga cttaagcaaa atctacttca aaaacgataa aagtctgact
     1201 gatttaagcc agcaagtttt cgacgactat agtgtaattg gcaccgccgt attggaatat
     1261 atcactcagc aaattgcccc taaaaatctg gataacccgt cgaagaaaga acaggaactg
     1321 attgccaaga aaaccgaaaa ggctaaatat ctgagcttgg aaacaatcaa actcgcactg
     1381 gaagaattca ataagcatcg ggatattgat aaacaatgtc gttttgaaga aattctggcc
     1441 aattttgctg caattcctat gatattcgat gaaattgcac aaaataaaga taacttagcc
     1501 cagatttcta ttaaatatca gaaccagggt aaaaaagatc ttttgcaggc gagtgccgaa
     1561 gatgatgtaa aggctattaa agatctgctg gatcaaacta ataatttgtt acataaactg
     1621 aagattttcc atatctcgca gtctgaagat aaagctaata ttttggataa agacgaacat
     1681 ttctatctgg tatttgaaga atgttatttc gaattagcaa acattgttcc gctgtataac
     1741 aaaattcgta attatattac tcaaaaaccg tattcagatg aaaaatttaa actgaatttt
     1801 gaaaatagta cattagctaa cggatgggat aagaataaag aacctgataa tacagcgatt
     1861 ttattcatca aagacgataa atactatttg ggagttatga ataagaaaaa taataaaatc
     1921 tttgatgata aagctattaa agaaaataaa ggagaaggtt ataagaaaat agtttataaa
     1981 ttgctgccag gagctaataa gatgctgcct aaagtatttt tcagtgctaa atctattaaa
     2041 ttctataacc cgtcagagga catcttaaga attcggaatc atagcaccca tacaaagaat
     2101 ggatctcccc aaaaaggata tgaaaagttc gaatttaata ttgaagattg cagaaaattt
     2161 attgatttct ataaacaatc tattagtaaa catcccgaat ggaaagattt cggctttcgt
     2221 ttctctgata cacaaagata taatagtatt gatgaatttt atagagaagt agaaaatcag
     2281 ggctataagc tgacgttcga aaatatttca gaatcctata tagatagtgt agttaatcag
     2341 ggtaaattgt atttatttca gatttataat aaagactttt ctgcctattc taaaggacgt
     2401 cctaatctgc atacactcta ttggaaagct ttattcgatg aacgtaacct ccaagatgta
     2461 gtatataagc tcaatggaga agcagaattg ttctatcgca agcaaagtat tcctaaaaag
     2521 atcactcatc ctgctaaaga agccattgct aataagaata aagataaccc taagaaagaa
     2581 tcagtatttg aatatgattt gataaaagat aaacgtttca ctgaagataa gtttttcttc
     2641 cattgtccga taacaattaa ctttaagtcc agtggagcaa ataaattcaa tgatgaaatc
     2701 aatctgcttc ttaaagaaaa agcaaatgat gtacatattt tgtctattga tagaggtgaa
     2761 cgtcaccttg catattatac tttagtggat ggaaagggaa atatcatcaa gcaggatacc
     2821 tttaatatta tcggaaatga ccgtatgaaa acgaactatc atgataaatt ggctgctatt
     2881 gaaaaggatc gtgatagcgc tcgtaaagac tggaagaaaa ttaataatat caaagaaatg
     2941 aaagagggat atctgtcgca ggtagtacac gaaatcgcta aactggtaat tgaatataat
     3001 gccatcgtag tatttgagga tctgaatttc ggtttcaagc gtggacgttt taaagtagaa
     3061 aagcaagtat atcagaaatt agaaaagatg ttaattgaaa aattaaatta tctggttttc
     3121 aaagataatg aattcgataa aaccggaggc gtcttgagag cctatcagtt aactgcccct
     3181 tttgaaactt ttaaaaagat gggtaaacag acaggaatta tttattatgt gccggccgga
     3241 tttacatcta aaatctgccc ggtgactggt ttcgtgaacc aactgtatcc gaaatatgaa
     3301 agtgttagca aatcccagga attctttagc aaatttgata aaatatgtta taatttggat
     3361 aaaggatatt ttgaattcag ttttgactat aaaaattttg gagataaagc cgcaaagggt
     3421 aagtggacta ttgctagttt cggatcgcgc ttgattaatt ttcgtaattc agataaaaac
     3481 cataattggg atactcgtga agtgtatcct accaaagaac tggaaaaatt gttaaaggac
     3541 tatagtattg aatatggaca tggtgaatgt ataaaagcag caatatgcgg agaaagtgat
     3601 aagaaattct ttgcaaaatt gactagcgta ctgaatacta tattgcaaat gcgtaatagt
     3661 aaaacaggaa cagaactgga ttatctgatc agccctgtag cagacgtaaa cggcaatttc
     3721 tttgatagtc gccaagctcc taaaaatatg ccgcaagacg ctgatgcaaa tggagcatat
     3781 cacattggtc ttaagggact gatgctgctg ggtcgcatta aaaataatca ggaaggtaaa
     3841 aaactgaact tggtaatcaa aaatgaagaa tattttgaat tcgtacagaa tcgtaataat
     3901 taatgaactg cacttgcttt gataattaat gataaacaat ctaaaagcac tctaatcgtt
     3961 atcggagtgc ttttagatta ctaatcaaat tgcttctact aattgcctat cttccagtga
     4021 tggaacagca tttgtgcatt ggctgcaaca atagccttga tctggaagaa gcaatgaaag
     4081 ctgctgttaa gtctccgaat caggtattgt tcctgacagg tgtattccca tccggtaaac
     4141 gcggatactt tgcagttgat ctgactcagg aataaattat aaattaaggt aagaagattg
     4201 taggataagc taatgaaata gaaaaaggat gccgtcacac aacttgtcgg cattcttttt
     4261 tgttttatta gttgaaaata tagtgaaaaa gttgcctaaa tatgtatgtt aacaaattat
     4321 ttgtcgtaac tttgcactcc aaatctgttt ttaacatatg gccctcccga aaggggggcc
     4381 ttttttcgtt ttggtcctac ttgtgcctgt tctatttccg aagataaagc taaaagttct
     4441 tatctttgca gttaatttct actcttgtag attactatat ttcccttccc tttcagagga
     4501 tccaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcttgc
     4561 ggaaagagca taatcacaag gacgggaagc aggtaggcaa acagtatact tttctgaaag
     4621 gtacatatga tttgtatgag accgtttatt tcaatgaaga aggtttgcag gacggtgaat
     4681 attcgtccgt gtttacgttt ggctcgccac gtatactggg tagttataag aatggaaaga
     4741 agaatggtcg gtggactact tttgctgaaa gcggagatac gctgatgatt gaaacctatc
     4801 tggatggaaa agaagatgga ctccatgtct catttgccaa cggaagtggg gtaaggcaga
     4861 aagaatatta tatgaagaac gatcgtaaag acggtttata ccgtgaatat aatctggaaa
     4921 atggtgaatt gagatatgaa gctacctatc aggccggcag attgcatggc aaggaacgac
     4981 ggctgatagt gagcaaccgc tttgattatt gggaaacttc tacctatgtg aacggaaggc
     5041 agaacggacc gttcgaagct cgttatgtaa agaatgacca gcttcgtgaa tgtggggaat
     5101 ataagaatgg tcgccgtgta ggacgatgga aacgctacac tattgatggt aaactggaaa
     5161 aggaatggga tgaaaactaa ccggtagatg aaaggtcagg gcataaaaaa aggagcaacg
     5221 ccttgctcca acctttgtta accttaaatc taatactatg aaaaacacag tgcaaagata
     5281 cggacttctg tgaaatctgc aaattatcca agtaaaacgc tatgttttat aacattgatt
     5341 aagtgtttgt tttacgcttt atacttttgt taatactttc cgatggtctg aaagaggact
     5401 gctttccttc tttttagaat aataaatagc tgaagttaga gaactttaca gtattatttc
     5461 ttatttttgt tcttaattgg attacagaaa gaactatgaa atgatttatg gctgccatca
     5521 gtaaattccc ggatataaag ctgattgata aagcggatgc tgcgtgggaa cgtggtccgg
     5581 cagagataga aatggatagt atgcttcgga ggcatcctaa gattgatgct gtgtatgccc
     5641 ataatgaccg tatcgctccg ggtgcctatc aggcagcaaa gatggcaggg cgggagaagg
     5701 aaatgatttt tgtcggcata gatgccttgc cgggtaaggg aaacggactg gaactggttt
     5761 tggacagtgt gctggatgcc acctttatct atccgaccaa tggcgataag gtactgcaac
     5821 tggctatgga cattctggag aagaaaccct atcccaaaga aacggtgatg aataccgctg
     5881 ttgtggaccg taccaacgca cacgtcatgc agttgcagac tacacacatc tccgaactcg
     5941 ataaaaagat tgaaacgctc aacggacgta tcggtggata cctctctcag gtagctacac
     6001 aacaggtcgt tttatacggc agtctgatta tccttttatt ggtagccggc ttattattgg
     6061 tcgtttataa atcactccgc tctaagaatc gcttgaataa agagcttttt aagcagaagc
     6121 agcaattgga agagcagcgt gacaaactgg aagaacagcg tgaccaattg atacagctct
     6181 ctcatcaact ggaagaagct acccatgcca agctggtctt tttcaccaat atttctcacg
     6241 acttccgtac tccgttgaca ttggttgccg acccggtaga acatttattg gcggacaaga
     6301 cattgagtgg agatcagcac cggatgctca tgctgattca gcgaaatgtg aatatccttt
     6361 tgcgcctggt caatcagatt ttggatttcc gtaaatatga aaacggcaag atggaatata
     6421 ctccggttac ggtggatgtc ctttcttctt tcgaaggatg gaatgagtct tttcaggcgg
     6481 cagcccgtaa gaagcatatc cataattccg aacaactgga ggctttaagg ccgaccgctt
     6541 gtatgaatcc atcaaaattc gttttctcta cgttggattc cttgttgctc atattgtgat
     6601 gataatttct acaaatatag tcattggtaa ctatctatga aactgtttga tacttttata
     6661 gttgattaaa cttgttcatg gcatttgcct taatatcatc cgctatgtca atgtagggtt
     6721 tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac cacctcaaga ttacccggag
     6781 tgtttcagcc gggtaatctt gttacttatc cttgattttc cctttaaggg cgtttataat
     6841 ccaccctttc ggattgttct tttctcgtga ttttccgttt aggagagcca gttctccgat
     6901 aaggtcggtt atcttttctt gtgccgttat gaatgtctct ttgttccggt ttatctcttc
     6961 cgatgtgaag ccgcaggaat aacggaggta ctcgtacaca tggctgtcta tctgatatcg
     7021 tgctgtaacc tttgcttgca attctttccc ttccagttct tcatctctga actgtggttg
     7081 atagaccggg tagaacctaa acccggtcac cttgctacgt ttgttgtttg ggttttcacg
     7141 cactttcacg tagttgaaag tgtatgggca gctctcatca agcgcagcct ttgctggttt
     7201 caaaactctt tcctcaaaat gatctattct gtcttttccg ttcttgtcct tgtatttgtc
     7261 cgcaggtatg ccaagacggt ctttcaagtt ttcaagggat atatccaaag ggtacacctg
     7321 cccactcatt aacatgtaaa acctaaggga atacccagta ggtagcgcaa gagccttgtt
     7381 taactcaaat tctctgtacc ccttggcgaa cttggtaaac acatcccaaa ggtcgttaga
     7441 cacacgaaag gttataatgc cagtgcgttt tttatatttt gggttagata taaatccgca
     7501 cttccaccat tcctcgtcat cttcgtaagt aaaaaaacgt cctgccaaag aatcgagtgc
     7561 ggctatgatt tcattgtaat cacgtccgga aaaaataacg tcagatacat gcatttgtgc
     7621 atcaacatcc caaagtccat gctcaaactt gcgtttcgtg cctgcataat ctttcagttt
     7681 tactcccttt agttcagctt ggcacgcttc cagtatgcga agaactattc tttgttcgtg
     7741 tatggacatg tcttgctgtt ttgaccatgt atatacccat gaaacaacaa catctttatt
     7801 cttggtaata ggtagtttct ttttcataaa cgattgattt gctgcaaata tacacaatcc
     7861 ccacattaaa acaaaaaaag aggggtaaaa ctcccaaagt gtggggacta atttactttt
     7921 aaactcccaa agtgtgggga ctaaactccc aaagtgtggg gaaacgcatg ttctaatccc
     7981 tttatttatg ggcgtttgcg aagaagtgat agagcatatt acagacgatg cttataacag
     8041 acaaggggaa tttatagagc taagtggttg atttactgtc ggaaaaagaa aaacgatagg
     8101 gaaaaagagc aaagcaaaac caaccaacgg gcggtaggat tggaaacggg tttttatggc
     8161 gtgtacacgt ttgtgtacaa aatgtttgta ttttattgaa atatagctat ctttgtgctg
     8221 aatttaaaag caaacagatg gcccaagaat gtaaagtcgg ctgtttttgt tttatttaag
     8281 ataatacaac cactacataa taaaagagta gcgatattaa aagaatccga tgagaaaaga
     8341 ctaatattta tctatccatt cagtttgatt tctcaggact ttacatcgtc ctgaaagtat
     8401 ttgttggtac cgaggacgcg taaacattta cagttgcatg tggcctattg tttttagccg
     8461 ttaaatattt tataactatt aaatagcgat acaaattgtt cgaaactaat attgtttata
     8521 tcatatattc tcgcatgttt taaagcttta ttaaattgat tttttgtaaa cagtttttcg
     8581 tactctttgt taacccattt cattacaaaa gtttcatatt tttttctctc tttaaatgcc
     8641 atttttgctg gctttctttt taatacaatt aatgtgctat ccactttagg ttttggatgg
     8701 aaataatacc taggaatttt tgctaatata gaaatatcta cctctgccat taacagcaat
     8761 gctagtgatc tgtttgtatc taataacatt ttagcaaaac catattccac tattaaataa
     8821 cttattgtgg ctgaactttc aaaaacaatt tttcgaatta tatttgtgct tatgttgtaa
     8881 ggtatgctgc caaatatttt atatggattg tggctaggaa atgtaaattt cagtatatca
     8941 tcatttacta tttgatagtt aggataattt aagagcttat tacgagttac ctcacataat
     9001 ttagaatcaa tttctatcgc cgttacaaaa ttacatctct ttaccaatcc agcagtaaaa
     9061 tgacctttcc ctgcacctat ttcaaagatg ttatcttttt catctaaact tatgcaattc
     9121 attatttttt ctatgtgata ttttgaagta ataaaatttt gactatcttt tatatttact
     9181 ttgttcatta taacctctcc ttaatttatt gcatctcttt tcgaatattt atgttttttg
     9241 agaaaagaac gtactcatgg ttcatcccga tatgcgtatc ggtctgtata tcagcaactt
     9301 tctatgtgtt tcaactacaa tagtcatcta ttctcatctt tctgagtcca ccccctgcaa
     9361 agcccctctt tacgacataa aaattcggtc ggaaaaggta tgcaaaagat gtttctctct
     9421 ttaagagaaa ctcttcggga tgcaaaaata tgaaaataac tccaattcac caaattatat
     9481 agcgactttt ttacaaaatg ctaaaatttg ttgatttccg tcaagcaatt gttgagcaaa
     9541 aatgtctttt acgataaaat gatacctcaa tatcaactgt ttagcaaaac gatatttctc
     9601 ttaaagagag aaacaccttt ttgttcacca atccccgact tttaatcccg cggccatgat
     9661 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9721 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9901 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    10081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10141 catgtaacta gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10201 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10261 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10321 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10381 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10441 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10501 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcataa
    10561 cgcgtcaatt cgagggggat caattccgtg ataggtgggc tgcccttcct ggttggcttg
    10621 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
    10681 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
    10741 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
    10801 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
    10861 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa cggaattgat
    10921 ccggccacga tgcgtccggc gtagaggatc tgaagatcag cagttcaacc tgttgatagt
    10981 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca
    11041 tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag
    11101 ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca
    11161 acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt
    11221 aaagctttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc
    11281 ccttagagcc tctcaaagca attttgagtg acacaggaac acttaacggc tgacatggga
    11341 attcccctcc accgcggtgg cttaagaccc actttcacat ttaagttgtt tttctaatcc
    11401 gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa
    11461 ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc
    11521 tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc
    11581 gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg
    11641 attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc
    11701 atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg
    11761 ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc
    11821 taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac
    11881 acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag
    11941 ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatattcg tttaatatca
    12001 taaataattt attttatttt aaaatgcgcg ggtgcaaagg taagaggttt tattttaact
    12061 accaaatgtt ttcggaagtt ttttcgcttt tctttttcta tcgtttctca gactctctta
    12121 gcgaaaggga aagaaggtaa agaagaaaaa caaaacgcct tttctttttt tgcacccgct
    12181 ttccaagaga agaaagcctt gttaaattga cttagtgtaa aagcgcagta ctgcttgacc
    12241 ataagaacaa aaaaatctct atcactgata gggataaagt ttggaagata aagctaaaag
    12301 ttcttatctt tgcagtctcc ctatcagtga tagagacgaa ataaagacat ataaaagaaa
    12361 agaca
//
