LOCUS       sequence-368162-       10373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Swi6 full length with L->A mutated in nuclear localization signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Belanger KD, Yewdell WT, Barber MF, Russo AN, Pettit MA, Damuth EK, 
            Hussain N, Geier SJ, Belanger KG
  TITLE     Exportin Crm1 is important for Swi6 nuclear shuttling and MBF 
            transcription activation in Saccharomyces cerevisiae.
  JOURNAL   BMC Mol Cell Biol. 2022 Feb 21;23(1):10. doi: 
            10.1186/s12860-022-00409-6.
  PUBMED    35189816
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Mol 
            Cell Biol."; date: "2022-02-21"; volume: "23(1):10. doi"; pages: " 
            10.1186/s12860-022-00409-6"
COMMENT     Sequence Label: KBB441
FEATURES             Location/Qualifiers
     source          1..10373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     56..76
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(466..484)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(505..521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(505..521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(518..540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    529..545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(553..559,560..577,578..583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    598..619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(736..753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(907..1495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(987..1006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1666..2457,2458..2526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2289..2308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2527..2631)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2668..3171
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     3213..3231
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3269..3291)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3391..3410
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(3904..4998)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4999..5406)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(5707..6162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5844..5865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6056..6075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6288..6310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6302..6319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6303..6319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6326..6345
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6326..6344
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6377..6393
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6377..6393
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(9773..10373,1..116)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(9806..9834)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
       61 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaaggat
      121 ccggctgcta acaaagcccg aaaggaagct tcggtatact tatttttact cccccttctg
      181 tgttttcttc tttttttttt tttttttttt ttttggaatt tactgttgtt tctttcgtgg
      241 ctgtttctta atcttatacg tacctttatc tatttccgta cagttgtaat ctatcttctt
      301 tagatcttgc cattttttat gcggtatatc ccaatggcat tttttagcta cgagactggc
      361 cacgtcatca cagaatccca acaggtttaa gtcaaactct gcatgtgtaa ccatatccct
      421 attgatcaaa atctgcggaa catgtgaagg taccagcttt tgttcccttt agtgagggtt
      481 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2641 ccgaaaagtg ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat
     2701 tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat
     2761 agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt
     2821 tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga
     2881 agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc
     2941 tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat
     3001 tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt
     3061 actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat
     3121 tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag
     3181 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     3241 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3301 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3361 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     3421 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
     3481 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
     3541 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa
     3601 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
     3661 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
     3721 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
     3781 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
     3841 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
     3901 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
     3961 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
     4021 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
     4081 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
     4141 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
     4201 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
     4261 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     4321 agaatcaatc aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc
     4381 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
     4441 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     4501 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     4561 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     4621 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     4681 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     4741 actaccggta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
     4801 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc
     4861 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     4921 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
     4981 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga
     5041 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
     5101 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
     5161 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
     5221 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
     5281 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     5341 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     5401 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca
     5461 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac
     5521 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt
     5581 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct
     5641 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     5761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     5821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     5881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     5941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     6181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6301 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
     6361 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcagcgtt tacgctatcg
     6421 gcatcatcaa taaattcagg tacctcatcc acaggctcgt atgatgttgt ttggtctttt
     6481 ggggctaggc cttcgacttt atcattatta tcgttttcca aatcgcttgt ctggtgatca
     6541 acatcttcat ccttaatctc ctcgctatca tccgcaaaat ctgcataatc taacgttttc
     6601 tcgtcgctgg aaccaatttc accctttgcc aatttactca aataaatttt gcaatactta
     6661 attcgttttt gtaatgaatt tgtactcgtt tcacttatat tttccttcca caggtgtaaa
     6721 atgcatgaaa ataaatcaat acaacaccaa agtgatcttt ttaacataac atcccaggga
     6781 ccatctttta gctgtttcag tttctcgtta tacaacgaca ttgtaaaatt tagcatataa
     6841 attttggctt tttcttgatc atgtattagc gtgttcatat cgtgtaagct tttgtcactg
     6901 tcttctgctt cactctcgcc gccaatctcc ttcttaaacg cttcaatagt atcaagaagt
     6961 tctgtcgcta gtgcggtcat ctcttgcgac cgatcttctt cactcagtat caattccacc
     7021 gcatataact gaagatagta tccgatgatc cctaacccaa ctttgtcaaa atccttcgct
     7081 gtagcgacaa cccttgcagc gttactagcc atagaattta tttaaaatgt ggacaattag
     7141 tttctaaaaa ttgaatcaaa taaaataaga aagcttattg ttcctttttt tgtcttcacc
     7201 tggaccgttt ggataagatc attcaccaaa tcttagtttt tttttttagt ttccttctcc
     7261 aacgatttca caataataaa aaaaaacaac aaaaacacga ttgagaaaca tcaatatgaa
     7321 ctaagcaata gctaaaaaaa aaaagaataa taaaggggaa cacagtataa ttctcgagag
     7381 gatggcgttg gaagaagtgg tacgatactt aggacctcac aatgagatcc ctttgacact
     7441 cactagagac tcggagactg gccatttcct cctgaaacat tttctgccca ttttgcagca
     7501 atatcatgac acggggaata ttaacgagac caaccccgat agtttcccca ctgatgagga
     7561 aagaaataaa ttactggcac attatggaat tgctgtaaat acagacgacc gaggtgagtt
     7621 atggatagag ctggaaaaat gtttacaatt attaaacatg ctaaatttat tcggtttgtt
     7681 ccaggatgca ttcgaatttg aggagcctga aacagatcag gatgaagaag atcccagcca
     7741 ttcgaaacta ccagaaaaca agaccaaaag tgaaaattct aaggataata tcagctcaaa
     7801 gaggattaat aatttacaag atatgagcct ggattctgat gcacacagag aattaggctc
     7861 tcctttaaaa aaactaaaaa tagatacctc tgtaatagat gctgagagtg actccactcc
     7921 gaatactgcc agaggcaagc ctaacgatga tattaataag ggccctagcg gcgacaatga
     7981 aaataatggc actgatgaca atgacagaac cgctggacct atcataacat tcactcatga
     8041 cctaacttct gactttttaa gcagtccact gaaaatcatg aaagcactac cttctccagt
     8101 tgtaaatgat aatgaacaga agatgaaatt tccagaaatt caagaaatgc ctacatccct
     8161 taataatgac agcagtaata gaaattcaga aggggggagc tcaaaccaac aacaacagca
     8221 cgtatcattt gatagccttt tgcaagaggt aaacgacgct tttcctaata ctcaattaaa
     8281 tcttaatatt cctgtagatg agcatggaaa cacaccatta cattggctga cttcaatagc
     8341 aaacctggaa ttagtgaaac acctggttaa gcatggttca aacagattat atggtgataa
     8401 tatgggggag tcatgcctag tgaaagctgt caaatcagta aataattacg actctggtac
     8461 ttttgaggca cttctagatt atttatatcc atgtttaatt ttggaagact caatgaatag
     8521 aacaattttg caccatatca ttattacgtc tggtatgact ggctgttcag cagctgcgaa
     8581 atattattta gatattttaa tgggatggat tgtcaagaaa caaaatagac ccattcaaag
     8641 tggtactaac gaaaaagaaa gcaaaccgaa tgacaaaaat ggggaaagaa aggactctat
     8701 actagaaaat ttggacctga aatggataat agcaaacatg ctcaatgcgc aggactctaa
     8761 tggcgatact tgcttgaaca ttgcagcaag attgggaaac atttcaatag tagacgcttt
     8821 attagattac ggtgctgacc catttattgc aaacaaatca gggttgagac ccgtggattt
     8881 tggggcaggt acttcaaaat tacaaaatac gaacggcggt gacgaaaact cgaagatggt
     8941 ctctaagggt gattacgacg gtcaaaaaaa tggaaaggcc aaaaaaataa ggtctcaact
     9001 gttgaaaaac ccacctgaaa caacttcgtt aattaatgat gtccaaaatt tactgaattc
     9061 aatctcgaaa gattatgaga atgaaacagt gcaatataat gagaaattag aaaaactaca
     9121 caaggaattg aacgaacagc gagaagaatt agctaattct agagaacaac tagcaaatgt
     9181 gaagcaattg aaagatgaat actcactaat gcaagagcaa ttgaccaatt taaaagcagg
     9241 aattgaagaa gaagaggaaa gttttaggga agagagcaaa aagctaggaa taattgcaga
     9301 tgaaagttca ggtattgatt gggactctag tgaatatgat gcagatgaac ccttcaaagt
     9361 agagttcctt tcagatttct tggaggataa attacaaaag aattatgaag gcgatatttc
     9421 caaactatta gaagcggagt cgaaagagca gataatggaa cagatacgaa atcaacttcc
     9481 cgcagaaaaa atccaatcga tgcttccacc aacggtctta ttgaaggcaa gaataaatgc
     9541 atacaagcgg aatgataaac acctaaccaa tgtattggat acaatcagca ccaaacaatc
     9601 agaattagag aataaattta gaagagtgtt atctttatgt ttgaaaatcg acgaaaataa
     9661 agttgacaat atgcttgatg gtttgttaca ggccatatct tctgaagacc cacaagacat
     9721 tgacactgac gaaatgcaag attttttaaa aaagcatgct tcaggtcgag ctatggctag
     9781 caaaggagaa gaactcttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     9841 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     9901 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     9961 tactttcact tatggtgttc agtgcttttc aagatacccg gatcatatga aacggcatga
    10021 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
    10081 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
    10141 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttgggc acaaattgga
    10201 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
    10261 agctaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
    10321 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc att
//
