LOCUS       sequence-368165-       12253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal truncation of Pom152 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Ken Belanger Lab
  TITLE     Belanger lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: KBB528
FEATURES             Location/Qualifiers
     source          1..12253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    87..108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(123..128,129..146,147..153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    161..177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     185..201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     185..201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        222..240
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     268..284
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     269..285
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1663..1689
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1705..1731
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1747..1773
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3784..4494
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3811..3839)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4434..4454
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6566..6582)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6566..6582)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6614..6633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6615..6633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6640..6657)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6640..6656)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6649..6671)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6797..7252
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6884..6903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7094..7115
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7553..7960
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7961..9055
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9549..9568)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9668..9690
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9728..9746)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9788..10291
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10328..10432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10433..10501,10502..11293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10651..10670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11464..12052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11953..11972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12206..12223
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
       61 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      181 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
      241 gaacaaaagc tggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
      301 aattcctgca gcccggggga tccacagttg acaacgatct caagattggt tgggatttaa
      361 atcctatagc agtgcatcag aagaagcaaa aaggccagtg gacagtcatt aagtttatca
      421 ccagaaaggg cttccagacc atcacaggtg aagagaaaga aaaaaaataa tgcgctggta
      481 ttatagaaat agagtttcat tatataagaa catataggaa gaatacatta ttcctatcta
      541 gggcatcaaa taattgcaac cacttaatgt taacttcctt cataattcaa aacattatgt
      601 tctatagcac tatgtatttt atgtcattat atacgtagtc aataacttta tacgtttttt
      661 ttattgaaca aaagtaagaa ttgcttaaga cataataaga ggaaactcat cgaaccaatt
      721 caaaacggat tatagattta tcataccaga tacgtttatc aggaggcttg tataatggag
      781 cacagatata acgtgtttaa tgatactcca cgagggaacc attggatggg cagttctgtg
      841 tcaggatcac cacgtccatc ttatagcagt cgtcctaatg taaacacaac acgacgcttc
      901 caatatagtg acgatgagcc cgctgagaaa atccgacctt tacgctcgag gagctttaaa
      961 agtactgaaa gtaacataag cgatgaaaaa tcaaggatat ctgaacgtga cagcaaagac
     1021 cgctacatta atggtgataa aaaggtagac atttattcac tgcctctaat atcaaccgat
     1081 gttttagaaa tttccaaaca aaggacattt gcggtgatat tgtttttaat aattcaatgt
     1141 tataaaatat atgacctggt aatacttaaa tcggggttac cgctttcggg tttactgttt
     1201 aagaactatc gatttaattt catatccaag tatttcatta tagattcctt cttcctgtat
     1261 gttttaccat cctttaacat tcctaggttg acttttaagc catgggttgt ttatctccag
     1321 attttagcta tgctactatt gaacattttc atatcaagcg atcacgagtt cgttttgatt
     1381 tcattaatta tgaccacatg gagaaaactt tatacgaaag aattaagtgt aacaggttca
     1441 gccattaacc atcatcgtat ttttgattct tccgctcatt ttaagggtgc tctaacaatc
     1501 aagatcctac ctgaaaacac agctatgttt aatcctcttc atgaatcata ttgtttgccc
     1561 atggatacca atctttttaa aattaactca attgacgtac ccatcagaat aaactcaact
     1621 gaagagattg aatatatcga gttggaatat agagatcttg gttacccata cgacgtccca
     1681 gattacgcta gcggtgatct tggttaccca tacgacgtcc cagattacgc tagcggtgat
     1741 cttggttacc catacgacgt cccagattac gctagcggtg atctttacac aaattcggta
     1801 gagttacggt ctttgagtaa gaaagacttc aaaatcatcg ataaccccaa atccttttta
     1861 aaaaaagatc agtcagttct aaagtcacat tcaaacgatt ttgaagaagg cagtacaatt
     1921 cgttatttgg ctgtaactct tcaagatatt ggcttttatc aaatcaaaaa aattgttgat
     1981 tcaaaaaaac tgaatttaaa gatccatcag tcacacttgg ttgtacccta ctgtccaatt
     2041 gcatctatta ctggaactgg aagcaatgat agatgtattg gcgattcaga taatgtttct
     2101 tttgaaattc aaggtgtgcc cccaatgaaa ttagcatact ctaagatcgt taatggtcaa
     2161 actttttcat atgtagactc cagcttgcaa ccagaatatt ttgagtctcc tttgcagtca
     2221 agcaaatcca aacaaagttt tactcaagga gaactaaacg atttgaaatg gggaagaaac
     2281 caacctgtta atattaattt agactcttcc attacccagg acggcaaatt tgcatataaa
     2341 attgataaaa taactgatgg tctgggaaat gtcgtggatt ttacatctct ccctgaagaa
     2401 ttaaaaaagc gctatgatct atcttataat ttcaatgttc atgaggttcc acgtgcagcg
     2461 ctagaagaaa gatttgatcc aaagtcaccc acaaaaaggt ctattgccat cgtgtttgaa
     2521 gaaatcaaaa actggatttc agacattcca tatgttatta gcctatccta tactgatgca
     2581 caagacaagt cgaaaaagat aatgaatgtt actacagatt ctttgaccaa agtgttgcaa
     2641 gcggatcttc cggggtcata taacttagaa tatattgaat ctaagttttg tccaggtgaa
     2701 attgtgggta aatcaaacgt tcttgtaact atgccagttg cacctactat ggaagttaaa
     2761 tcatttccaa tattggacca gtgtgtggga caggttggtc taaactttga actatctttt
     2821 actggggctc caccatacta ttataacact aaaatctata agcttgaaaa cggtgaaaga
     2881 aagttatatg atgcaaagag atacacctct gaaggtacga gaaatcgctt cagctatagt
     2941 ccacctaaag aaggtaacta tgaaatcgtg tttgatactg tttctaataa attattcact
     3001 gaaccaatca aattggaacc tgtaaaagaa tatactttca agacatcaat gagggtgaaa
     3061 ccaagcgcat cactaaaatt acaccatgat ttgaaactct gtttgggtga ccacagcagt
     3121 gtcccagtag cactaaaagg ccaaggccct tttacgttaa catatgatat cattgaaact
     3181 ttttctagca agaggaaaac ttttgaaatt aaagaaataa aaaccaacga atatgtcatt
     3241 aaaacaccag tctttactac tggtggtgat tatattctat ctttggtttc tatcaaggat
     3301 tctaccggct gtgtagttgg actcagccaa ccagatgcta aaatacaggt gaggagagat
     3361 attccatctg ctgcattcaa tttttttgaa cctatcaaag aagctaaaat taagcacggt
     3421 tctgtcactg aaattccgct caaattaagt ggagaggggc cattcaccgt taagtttaaa
     3481 cacatggatt acgatggaaa cattgtgaaa gaatttgaaa acaaatttca gaatagttat
     3541 aagcccgctt tgaaagtaag taaagaaggg ctttaccaat tggtcgatat tcgtgactca
     3601 agttgccaag gtaatgtgat ttaccggaac agtctgtata aggtatcttt cttggagaag
     3661 ccaaaatttg caattcaaga caatcatcac attacaaaag taacggaaaa tttattctct
     3721 aaagaagaag tctgtcaagg gatggaaggt acggttgatt tggctctatt tggttctcca
     3781 gctagcaaag gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt
     3841 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     3901 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     3961 gtcactactt tcacttatgg tgttcagtgc ttttcaagat acccggatca tatgaaacgg
     4021 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     4081 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     4141 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tgggcacaaa
     4201 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     4261 atcaaagcta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     4321 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     4381 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     4441 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaaggatcc
     4501 ggctgctaac aaagcccgaa aggacggtct atagaggtgt gttaaaagca gacttttgaa
     4561 catccacaga aattggctca atgacctatc cgaaaagaat acctataaat gcgtggtctg
     4621 aagttcatag ggtggcaagg ccactaatta ttactttcga cgcatataat acactatacg
     4681 ccacaaaact tcccgtcatg gaacaatact gcattgttgg gcgtaaatat ggtatcaagg
     4741 cgaatccttc gactttaaca aacaattttc cacacgtttt taaaaaactg aaggaagact
     4801 atccccaata cggaaaatat tctggtatca agcccgaaca atggtggtcc atcttgatta
     4861 ggaacgtctt cgcaccaaac gaaattcccg atgagatgat aaatgaaata ttaatgcggt
     4921 ttgagggctt cgattcgtat tttgtttatc cagatttgat caaattttta aaggatttga
     4981 agtcaagaca tcctgatgtt atcttaggaa tagtaagcaa tactgatcca attttttaca
     5041 aattattaaa gaatatcgga ttatttgaga cattttccgg tcacatttat ttatcatatg
     5101 agcttaatct cgccaaacct gatagagcaa tatttcaata tgcgcttgac gatattatat
     5161 ctaaacaacc tcatttactc gaaaagtaca ccagggagga aattctacag cattgctttc
     5221 acatcggaga tgaattaaag aacgatctcg aaggggcaga agctgccggt tggactggta
     5281 tactgttaga tagaaatgat aaatacgggt ttctttcaaa ctcgattagt aaaccaatgc
     5341 gggacgaata caagctgtca attgataaaa tagataataa ttcaataaac acttgggaag
     5401 ccaatactaa gcaaactgac acgttacagt tgagtgaaag aaagtacgtt gtttcgaatc
     5461 ttgaggtttt agaggaactc tttccctgag cgcctaacgt tacagtaaca aatatgtatg
     5521 atagatatta ttcatatccg ccagtaatat ttactatatc tttgaatggg tttgtttatt
     5581 aataatctac tatgtgtaaa tatatgatcc aagtattata cagtttagac actgattgtt
     5641 ttccagacgt tcaaaccgtc agtaccggta cttactaaac aacctgggat ttgcttatgc
     5701 catttgacat ctttaacttc cttttgccag tgaacaaaca ataattgtgg tggaatttct
     5761 tgtagctctt ttgtttcggc ggcctgttgt ttaatttcct catcgtcagc ttctacagac
     5821 aaatcccaca aagtcacagt attatcttct gagcctaccg caacgataga ctcatctaat
     5881 gggttgaatg caatggaagt aatggctccc ttatggaagt catattgagc aaccggttgg
     5941 acggcgtcag cattacttgg cgtaaactgt cttaaatccc atactcccca tgtaccgtta
     6001 tcgtcaccgc ttgctagcaa gtaaccaatt ttatcactcc aacttatgac atttacgtca
     6061 gtattagaag ctttaacaga gatagcaggt ttatgttttt ttgatcttgt gtcccaaatc
     6121 cttatatatc catcacatcc tgcggttgca aaaacggtgg attcagtgcg agaccactgg
     6181 atatcttcta tggatttatt gtttgaaaca gtaaatggtt gtttatcagt cacccatctc
     6241 gatgtgtgcc tttgggtaaa atatatttgt cctgagcaat cacctgatag taacgcacca
     6301 gtcttgatca atggtgacca atccaacccg tagccttcaa cattcccatg attttttaca
     6361 gtgtgaatag gacgcttagc agacttcgga atctgataac ctggtgtgga aaaagctttg
     6421 ctttgtggag ctagattgta tatataaaca tcaccatttt cgctcattgt agcggttaac
     6481 acctcttgat tagaaatggc aaaaggagaa acctttaatc tattggttgt atctctcaat
     6541 ggtatatttt cattctcaat gactggatcc actagttcta gagcggccgc caccgcggtg
     6601 gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     6661 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6721 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6781 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6841 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6901 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6961 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7021 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7081 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7141 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7201 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     7261 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatcgacc ggtcgaggag
     7321 aacttctagt atatctacat acctaatatt attgccttat taaaaatgga atcccaacaa
     7381 ttacatcaaa atccacattc tcttcaaaat caattgtcct gtacttcctt gttcatgtgt
     7441 gttcaaaaac gttatattta taggataatt atactctatt tctcaacaag taattggttg
     7501 tttggccgag cggtctaagg cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg
     7561 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc
     7621 ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa
     7681 attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg
     7741 agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca
     7801 tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt
     7861 ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc
     7921 agcaatatat atatatatat ttcaaggata taccattcta atgtctgccc ctaagaagat
     7981 cgtcgttttg ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct
     8041 taaagctatt tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg
     8101 tggtgctgct atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa
     8161 gaaggctgat gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt
     8221 tagacctgaa caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag
     8281 accatgtaac tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc
     8341 taaaggtact gacttcgttg ttgtcagaga attagtggga ggtatttact ttggtaagag
     8401 aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt
     8461 gcaaagaatc acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat
     8521 ttggtccttg gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga
     8581 ggaaaccatc aagaacgaat tccctacatt gaaggttcaa catcaattga ttgattctgc
     8641 cgccatgatc ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat
     8701 gtttggtgat atcatctccg atgaagcctc cgttatccca ggttccttgg gtttgttgcc
     8761 atctgcgtcc ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg
     8821 ccacggttct gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc
     8881 tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga
     8941 tgcagttaaa aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag
     9001 taccaccgaa gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga
     9061 ttctcttttt ttatgatatt tgtacataaa ctttataaat gaaattcata atagaaacga
     9121 cacgaaatta caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca
     9181 tacatttatc aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata
     9241 ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta acattaatat tgacaaggag
     9301 gagggcacca cacaaaaagt taggtgtaac agaaaatcat gaaactatga ttcctaattt
     9361 atatattgga ggattttctc taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga
     9421 cctgaccatt tgatggagtt taagtcaata ccttcttgaa ccatttccca taatggtgaa
     9481 agttccctca agaattttac tctgtcagaa acggccttaa cgacgtagtc gatatggtgc
     9541 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     9601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     9661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     9721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     9781 taggacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg atggaataat
     9841 ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt tagaaagtaa
     9901 ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat
     9961 ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga
    10021 tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc
    10081 tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga gcaagataaa
    10141 aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat
    10201 tttttcttta atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat
    10261 ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg
    10321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    10381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    10441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    10501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    10561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    10621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    10681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    11041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    11101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    11161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    11221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    11281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    11341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    11401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    11461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    11521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    11581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    11641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    11701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    11881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    11941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    12061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    12121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    12181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    12241 aatacgcaaa ccg
//
